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Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set B, 25-48) Invitrogen™

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set C, 49-72) Invitrogen™

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with UD indexes Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow—total RNA converted to sequencing-ready library within 4.5 hours
• Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA

This Collibri Stranded RNA Library Prep Kit supports fast library preparation within 4.5 hours for mRNA or whole transcriptome libraries with 1–25 ng of mRNA-enriched or ribo depleted RNA sample input. Multiplexing of libraries can be carried out using up to 96 single-indexed or unique dual-indexed primers, enabling single-read or paired-end sequencing. The kit is suitable for various-quality RNA samples, including FFPE samples.

Visual cues of process integrity
For maximum convenience, visual cues of process integrity are included throughout the library preparation workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

How it works
Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed or unique dual-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 either single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer or unique dual-indexed PCR primers that enable multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit, is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Ion PI™ Hi-Q™ Sequencing 200 Kit (2 sequencing runs per initialization) Ion Torrent™

The Ion PI™ Hi-Q™ Sequencing 200 Kit contains the most advanced reagents for sequencing using the Ion Proton™ System. The kit contains a novel sequencing enzyme that enables increased variant calling accuracy. When used together with the Ion PI™ Hi-Q™ OT2 200 Kit, the Ion PI™ Hi-Q™ Sequencing 200 Kit helps to significantly increase the sensitivity and specificity of your sequence analysis. Typical applications for the Ion PI™ Hi-Q™ workflow are exome sequencing, gene panel sequencing, and transcriptome analysis. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion Proton™ system. (For 8 initializations and 8 runs, see Cat. No. A26772.)

Note: an Ion Proton™ Wash 2 Bottle is required for use of this sequencing kit.

The Ion PI™ Hi-Q™ Sequencing 200 Kit provides:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Support for sequencing using the Ion PI™ Chip, enabling up to 80 million reads per run

Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, provides fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes in your research.

Single-day sequencing workflow
The fast sequencing and data processing capabilities of the Ion Proton™ System produce sequencing results in as little as a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.

Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q™ OT2 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Sequencing 200 Kit v3.

IonCode™ Barcode Adapters, 769-1152 Ion Torrent™

IonCode Barcode Adapters 769-1152 are 384 unique barcode adapters designed for optimal performance with Ion Torrent semiconductor sequencers. This set includes IonCode Barcode Adapters 0901-0996, 1001-1096, 1101-1196, and 1201-1296. When used in combination with other IonCode barcode adapters and the AgriSeq HTS Library Kit, users can pool up to 1152 amplicon libraries and then conduct multiplexed sequencing analysis, which helps increase lab efficiency and reduce sequencing costs per sample.

Key product features include:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance enables identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification

Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries enables more cost effective runs by permitting up to 1152 samples per run and helps significantly decrease the cost and handling requirements of sequencing. Each barcode was individually evaluated to verify no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to verify balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls.

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set D, 73-96) Invitrogen™

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Ion 520™ Chip Kit Ion Torrent™

The Ion 520 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 520 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times in as little as 2.5 hours for 200 bp sequencing.

The Ion 520 Chip Kit offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 3–6 million reads, depending on application
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.

SequalPrep™ Normalization Plate Kit, 96-well Applied Biosystems™

Clean up and normalize the recovery of PCR reaction products in one step with SequalPrep™ Normalization Plates. For use with 5 µl of PCR reaction, extract up to 25 ng of products per well within a 2-3 fold concentration range.
- For use in Next Generation Sequencing platforms for normalizing pools of PCR amplicons
- Skip tedius steps required for determination of yields and re-aliquotting
- When combined with SequalPrep™ Long PCR Kit, you will get the most sensitive and reliable method for long PCR product amplification and amplicon normalization.

dGTP BigDye™ Terminator v1.0 Ready Reaction Cycle Sequencing Kit Applied Biosystems™

dGTP BigDye® Terminator Cycle Sequencing Kit with the AmpliTaq® DNA Polymerase, FS. This Ready Reaction Kit uses the same dye set as the BigDye® v1.0 kits.

The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

Easily Integrate into Your Workflow
dGTP BigDye® Terminator Kits require no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Collibri™ 3´ mRNA Library Prep Kit for Illumina™ Systems Invitrogen™

The Invitrogen Collibri 3’ mRNA Library Prep Kit is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) platforms. It supports library preparation from polyadenylated RNA in ~4.5 hours. The protocol generates one fragment per transcript, resulting in accurate gene expression values. Sequences obtained are close to the 3’ ends of the transcripts, so the kit is suitable for varying-quality RNA samples, including formalin-fixed paraffin-embedded (FFPE) samples. The majority of inserts are greater than 75 bp in size, corresponding to final library fragment sizes ≥200 bp. Multiplexing of libraries can be carried out using up to 96 single-indexed primers.

For convenience, the Collibri 3’ mRNA Library Prep Kit provides color-coded components for visual tracking of library preparation progress. Inert dyes in the reagents provide visual confirmation of additions and mixing, but do not interfere with enzymatic reactions and do not compromise library prep and sequencing results.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems with Human/Mouse/Rat rRNA Depletion Kit Invitrogen™

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. Total RNA can be converted to sequencing-ready library within 6.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~ 150 bp for whole transcriptome libraries starting with sample inputs of 100–500 ng of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related prioduct, the Collibri Library Quantification Kit is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Genexus™ Sequencing Kit Ion Torrent™

The Ion Torrent Genexus Sequencing Kit provides the materials required to perform two initializations and automated sequencing of two multilane chips using the Ion Torrent Genexus Integrated Sequencer. These innovative sequencing consumables are stable on the instrument for up to two weeks after initialization, allowing flexibility to use the chip lanes according to sample throughput needs. Genexus Bottles 1, 2, and 3 are shelf-stable and the Genexus Cartridge has a minimized footprint, limiting the required storage space at -20°C.

As part of the Genexus Integrated Sequencer workflow, the Genexus Sequencing Kit:
• Automates sequencing for 200–400 base-read Ion AmpliSeq or Ion AmpliSeq HD libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability to sequence one to four chip lanes per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion AmpliSeq™ Library Kit Plus Ion Torrent™

The Ion AmpliSeq Library Kit Plus is designed for rapid preparation of amplicon libraries using Ion AmpliSeq panels. Ion AmpliSeq technology enables scalable, multiplex polymerase chain reactions (PCR) from 12- to 24,000-plex in a single well. Next-generation sequencing (NGS) libraries can be prepared using as little as 1 ng of starting material.

Key product features:
• Scalable and efficient library preparation
• Easy and robust protocol
• Barcoded sample multiplexing

Scalable and efficient library preparation
Using as little as 1 ng of starting material, highly multiplexed PCR amplification of thousands of genomic target regions yields superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. The primers contain proprietary modifications that enable removal of primer sequences during library preparation, thereby resulting in efficient target assessment during sequencing.

Easy and robust protocol
The Ion AmpliSeq Library Kit Plus uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The protocol is simple to use and enables high yield and uniformity, reliably and reproducibly. The resulting libraries are ready for downstream template preparation using the automated Ion Chef System (which also provides the option of automated library preparation using the Ion AmpliSeq Kit for Chef DL8) or the Ion OneTouch 2 System followed by sequencing. The intuitive Torrent Suite Software and optional Ion Reporter Software enables automation of data analysis.

Barcoded sample multiplexing
The Ion AmpliSeq Library Kit Plus includes reagents for generating amplicons using Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress Barcode Adapters 1-96 kits or IonCode Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost, and to allow for accurate sample-to-sample comparisons.

Ion PI™ Hi-Q™ OT2 200 Kit Ion Torrent™

The Ion PI™ Hi-Q™ OT2 200 Kit contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System (OT2) for Ion Proton™ System semiconductor sequencing. The kit is designed to deliver the highest quality template for Ion Proton™ next-generation sequencing using the Ion Onetouch™ 2 System. Use the Ion PI™ Hi-Q™ template and sequencing kits for improved variant calling accuracy. The Ion PI™ Hi-Q™ OT2 200 Kit contains template preparation reagents for sequencing 8 samples. The kit components include proprietary Ion PI™ Ion Sphere™ particles, buffers, PCR reagent and enzyme mixes, consumables, and primers.

The Ion PI™ Hi-Q™ OT2 200 Kit delivers:
• The latest advancements to data quality and accuracy using the Ion Proton™ System
• Increased sensitivity with improved target coverage and representation
• Support of Ion PI™ Chip to be sequenced with the Ion PI™ Hi-Q™ Sequencing 200 Kit

Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Trancscriptome Human Gene Expression Kit, enables fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes.

Automated workflow
The Ion OneTouch™ 2 System automates multiple steps to provide template preparation and enrichment. With minimal hands-on time required, it allows for preparation of multiple samples per day and significant time savings.

Notes
This kit is only compatible with the Ion OneTouch™ 2 System.
Use this kit with the Ion PI™ Hi-Q™ Sequencing 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Template OT2 200 Kit v3.

MagJET NGS Cleanup and Size Selection Kit Thermo Scientific™

Thermo Scientific MagJET NGS Cleanup and Size Selection kit, sufficient for 10 preps, is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.

The kit is suitable for cleanup or size selection of 5 ng to 5 µg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.

Highlights

Robust—reliable cleanup and size selection from different reaction mixtures and buffers
Flexible—user-defined size-selection of exact DNA fragment range
NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

Applications

DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.
Includes
• MagJET Magnetic Beads
• Binding Solution
• Wash Buffer (concentrated)
• Elution Buffer
• DNA Fragment Mix

Related Products
MagJET NGS Cleanup and Size Selection Kit
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