Shop All Sequencing Workflow Controls

Ion S5™ Controls Kit Plus (Ion Torrent™)

The Ion S5™ Controls Kit Plus provides library preparation and templating controls for use with the Ion S5™ and Ion S5™ XL Sequencing Systems. The kit offers greater experimental control and empowers you to assess sample quality, help save time and reagents, and maximize productivity. Each kit contains a CEPH genomic DNA control for up to 3 library preparations, a 200 bp human CEPH control library, and a 400 bp E.Coli control library, for up to 10 template preparations each. The Ion S5 Controls Kit Plus replaces the previous Ion S5™ Controls Kit (Cat. No. A27760).

Note:
This kit is only compatible with the Ion S5 and Ion S5 XL Sequencing Systems.

Ion PGM™ Calibration Standard (Ion Torrent™)

The Ion PGM Calibration Standard is a small panel consisting of known sequence content with comprehensive and uniform representation of long homopolymers up to 10-mers. The Ion PGM Calibration Standard can be used with the Ion 314, Ion 316, and Ion 318 workflows for de novo research sequencing applications to allow for greater accuracy of base-calling.

Ion PGM™ Controls Kit v3 (Ion Torrent™)

The Ion PGM™ Controls Kit v3 contains control genomic DNA and control libraries for 200 and 400 bp run configurations using the Ion PGM semiconductor-based sequencing platform. The kit provides for greater experimental control through the use of quality control reagents for the semiconductor-based sequencing workflow and allows you to assess sample quality, save time and reagents, and maximize productivity.

The Ion PGM Controls Kit v3 provides you with:

• Quality control tools for greater experimental control
• Significant time and cost savings through identification of suboptimal samples
• Support for 200 and 400 bp run configurations

Ion Library TaqMan™ Quantitation Kit (Ion Torrent™)

The Ion Library TaqMan™ Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library TaqMan Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ System sequencing workflow.

With The Ion Library TaqMan Quantitation Kit You Get:

• Precision— The TaqMan® Assay provides greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods.
• Sensitivity— The protocol supports quantitation of femtomolar amounts of fragment libraries.
• Simplicity— The validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation.
• Versatility— The highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range.
• Efficiency— qPCR-based methods use less material for quantification.

Quantitate Library Input for Use on the PGM™ System
The Ion Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in an Ion fragment library for use during template preparation in the Ion Torrent sequencing workflow. Complete with TaqMan® qPCR mix custom-designed for Ion library adapters and an Ion library control, the kit is a complete solution for determining the optimal amount of starting material needed for high quality sequencing results.

This Kit Includes:

• A custom TaqMan® Assay designed for Ion library adaptors
• An optimized Ion Library Amplification mix
• An adapted E. Coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190bp in size, providing essential components for quality control while maintaining flexibility to accommodate your laboratory needs.

Compatible With a Wide Range of Applications and Instruments
With the Ion Library TaqMan Quantitation Kit, most real-time instruments using either fast or standard cycling programs can be utilized to accurately quantitate genomic and amplicon libraries. These instruments include the Applied Biosystems 7900HT, 7300, 7500, ViiA 7, StepOne™, and StepOnePlus™ Systems. The kit has been validated for quantification of Ion Fragment Libraries. The Ion-specific E. Coli DH10B Library control included in the kit is recommended for use only as a qPCR control and not a sequencing control.

E.Coli DH10B Control 600 Library (Ion Torrent™)

The E.coli DH10b Control 600 Library provides a templating control for use with the Ion 520™ & Ion 530™ ExT Kit- Chef and the Ion S5™ and Ion S5™ XL Sequencing Systems. The kit offers greater experimental control and empowers you to assess sample quality, help save time and reagents, and maximize productivity. Each kit contains a 600 bp E.coli DH10b control library for up to 10 template preparations.

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL Sequencing Systems.
Use this kit with the Ion 520™ & Ion 530™ ExT Kit-Chef and Torrent Suite™ Software 5.2 or higher.

Ion S5™ ExT Calibration Standard (Ion Torrent™)

The Ion S5™ ExT Calibration Standard is a small panel consisting of known sequence content with comprehensive and uniform representation of long homopolymers up to 10-mers. The Ion S5 ExT Calibration Standard can be used in conjunction with the Ion 520™ & Ion 530™ ExT workflow for de novo research sequencing applications to allow for greater accuracy of base-calling for templates between 400 and 600 bp.

Notes:
This kit is only compatible with the Ion S5™ and Ion S5™ XL Sequencing Systems.
Use this kit with the Ion 520 & Ion 530 ExT Kit-Chef (A30670) and Torrent Suite™ Software 5.2 or higher.

Ion PI™ Controls 200 Kit (Ion Torrent™)

The Ion PI™ Controls 200 Kit provides sample preparation and sequencing controls for use with the Ion Proton™ System. The kit offers greater experimental control and empowers you to assess sample quality, save time and reagents, and maximize productivity. Specifically, the sample preparation and sequencing controls, designed exclusively for the Ion Proton™ System, help predict run performance and assure high data quality with each sequencing run. Each kit contains a CEPH genomic DNA control for up to 3 library preparations, a human CEPH control library for up to 10 template preparations, and Ion PI™ Control Ion Sphere™ particles for up to 3 test fragment sequencing runs.

The Ion PI™ Controls 200 Kit provides you with:

• Quality control tools for greater experimental control

• Significant time and cost savings through identification of suboptimal samples

• Support for up to 200 base read libraries

Notes:

This kit is compatible with the latest of Ion Proton™ consumables: the Ion PI™ Sequencing 200 Kit v3 and the Ion PI™ Template OT2 200 Kit v3.

Ion Sphere™ Quality Control Kit (Ion Torrent™)

The Ion Sphere Quality Control Kit furnishes a complete set of reagents for performing templated bead quality control using the Qubit 3.0 Fluorometer. This comprehensive kit contains calibration controls, buffers, and a probe mix for quality assessment of pre-enriched and post-enriched template-positive Ion Spheres. The powerful combination of the Ion Sphere Quality Control Kit and the Qubit 3.0 Fluorometer gives you greater experimental control and significant time and cost savings. By providing the confidence to generate high quality sequencing data with every run, this quality control process is ideal for those who are new to next-generation sequencing.

The Ion Sphere Quality Control Kit contains reagents for 20 quality control measurements. This kit is compatible with Qubit 2.0 and 3.0 fluorometers.

The Ion Sphere Quality Control Kit Gives You:
• Higher quality sequencing results through robust templated bead quality assessment
• A significantly quicker and more economical method for evaluating templated bead quality
• An increased level of experimental control and confidence

Rapidly Increase Your Proficiency in Semiconductor Sequencing
Quality control is essential for experimental success with new technologies. The Sphere Quality Control kit combined with the Qubit 3.0 Fluorometer offers a low cost, rapid, easy-to-use quality assessment solution, accelerating your proficiency in semiconductor sequencing. Don’t suffer by spending hours troubleshooting with “black-box" sequencers. Get high quality sequencing data the first time.

Enjoy Higher Confidence in Your Results
The Ion Sphere Quality Control Kit with the Qubit 3.0 Fluorometer brings greater experimental control to your science. For FFPE, LCM, or single cell applications where input material may be compromised, this robust assay determines sample quality prior to enrichment and sequencing, saving you time and money.

Benefit from a Quality Control Kit Designed for the Highly Accurate Qubit 3.0 Fluorometer
With state-of-the-art optical components, the capability for sophisticated data analysis, and an intuitive user interface, the Qubit 3.0 Fluorometer platform provides highly accurate and specific measurements not only of templated Ion Sphere Particles, but also of DNA, RNA, or protein. Through the use of dye-based quantitation, the Qubit 3.0 Fluorometer enhances your productivity by letting you avoid costly repetition of experiments.