Shop All Library Preparation Kits for Sequencing

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set C, 49-72) (Invitrogen™)

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

IonCode Barcode Adapters, 0501-0596 (Ion Torrent™)

IonCode Barcode Adapters 0501-0596 are 96 unique barcode adapters designed for optimal performance with Ion Torrent semiconductor sequencers. When used in combination with other IonCode barcode adapters and the AgriSeq HTS Library Kit, users can pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, which helps increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance enables identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification
Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries enables more cost effective runs by permitting up to 768 samples per run, and helps significantly decrease the cost and handling requirements of sequencing. Each barcode was individually evaluated to verify no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to verify balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figures below).

Collibri™ ES DNA Library Prep Kit for Illumina Systems, with CD indexes (Invitrogen™)

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set D, 73-96) (Invitrogen™)

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

IonCode Barcode Adapters, 0601-0696 (Ion Torrent™)

IonCode Barcode Adapters 0601-0696 are 96 unique barcode adapters designed for optimal performance with Ion Torrent semiconductor sequencers. When used in combination with other IonCode barcode adapters and the AgriSeq HTS Library Kit, users can pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, which helps increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance enables identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification
Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries enables more cost effective runs by permitting up to 768 samples per run, and helps significantly decrease the cost and handling requirements of sequencing. Each barcode was individually evaluated to verify no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to verify balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figures below).

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set B, 25-48) (Invitrogen™)

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Oncomine™ BRCA Research Assay, Manual Library Preparation (Ion Torrent™)

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms. The assay is designed to provide sensitive and comprehensive sample amplification of all coding regions of the human BRCA1 and BRCA2 genes. This version of the assay is for manual library preparation. For automated library preparation using the Ion Chef™ System, please see Cat. No. A32841.

Features include:

• Validated for use on Ion 318 and Ion 530 chips
• Detects SNVs, InDels, and large exon/gene deletions/duplications
• Compatible with as little as 10 ng input DNA from formalin-fixed, paraffin-embedded (FFPE) tissue or blood samples
• Highly uniform coverage across all coding exons and splice sites for efficient sequencing and accurate analysis
• Optimized primer design and amplification chemistry enable highly specific target enrichment
• Validated detection of somatic variants to 5% and lower
• Simple and fast workflow produces targeted libraries in 3.5 hours typically

The Oncomine BRCA Research Assay is a complete kit facilitating the amplification of the entire exonic region of both BRCA genes from FFPE tissue or blood samples with as little as 10 ng of input DNA. Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on either the Ion PGM™ or Ion S5™ sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatic workflows within Torrent Suite™ and Ion Reporter™ analysis software that utilize optimized variant calling parameters for SNV, InDel, and large exon/gene deletion/duplication detection. Samples can be processed quickly and easily, and variants detected and identified confidently in either germline or somatic DNA analysis pipelines.

BRCA1 and BRCA2 genes are tumor suppressor genes that code for proteins that are vital components of the homologous recombination pathway of DNA damage repair. BRCA mutations resulting in the deficiency of either gene have been shown to result in inefficient activation of this function and are linked to hereditary predisposition to cancer. Errors in the coding sequence of BRCA genes have been detected in germline and somatic mutations and also occur in tumor samples.

Genexus™ Primer Pool Tubes (Ion Torrent™)

The Ion Torrent Genexus Primer Pool Tubes kit contains 50 empty assemblies of the custom tubes required to deliver primer oligos into the automated library preparation reaction. Each assembly is serialized for traceability and can be used with Ion AmpliSeq and Ion AmpliSeq HD-based assays. Pipette an aliquot of panel primers from a custom or AmpliSeq-on-Demand panel in order to run the assay on the Genexus Integrated Sequencer.

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion AmpliSeq™ Library Kit 2.0 (Ion Torrent™)

The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels.

Scalable Multiplex PCR Reactions
The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer Tool, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation uses very small quantities of starting material per PCR. The resulting DNA libraries are ready for downstream template preparation using the automated Ion Chef™ System (which also provides the option of automated library preparation using the Ion AmpliSeq™ Kit for Chef DL8) or the Ion OneTouch™ 2 System followed by sequencing. The intuitive Torrent Suite™ Software and optional Ion Reporter™ Software enables automation of data analysis.

5500 SOLiD™ Fragment Library Barcode Adaptors 1-16 (Applied Biosystems™)

The 5500 SOLiD™ Fragment Library Barcode Adaptor Kit provides a complete set of 96 unique barcode adaptors and primers for cost-effective next generation sequencing of fragment libraries on the 5500 Series Genetic Analysis Systems. When used in combination with the 5500 SOLiD™ Fragment Library Core Kit, the 5500 SOLiD™ Fragment Library Barcoding Kit enables users to pool up to 96 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the next generation sequencing workflow for a wide range of applications, including targeted enrichment

Key Product Features:
• Efficient multiplexing—enables multiplexing of up to 96 fragment or paired-end library samples on a single flow chip with the use of robust molecular barcodes.
• Cost effective runs—allows more samples per run, significantly decreasing the cost and handling requirements of ePCR, enrichment, and deposition.
• Consolidated 5500 SOLiD sampling—enables pooling of samples prior to emulsion PCR (ePCR) and sequencing on the 5500 Series Genetic Analysis Systems.
• Versatile sequencing—substantially benefits applications such as whole genome sequencing of small genomes or targeted resequencing of enriched samples.

For Research Use Only. Not for use in diagnostics procedures.

Collibri™ ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set A, 1-24) (Invitrogen™)

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Oncomine™ Comprehensive Assay v3C (Ion Torrent™)

The Oncomine Comprehensive Assay v3 is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. It provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 Systems with the Ion 540 Chip to enable up to 8 samples (7 samples and one no-template control) per run.

When combined with Oncomine Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

Key features of the assay are:
• Enables analysis of variants across 161 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

From sample to answer
The Oncomine Comprehensive Assay is part of an NGS workflow that helps researchers to filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

The workflow has been adopted by large-scale national clinical trial research programs including NCI-MATCH (United States) and LC-SCRUM (Japan), and includes:
• Proven Ion AmpliSeq chemistry
• Automated library and template prep on the Ion Chef System
• Scalable sequencing on the Ion GeneStudio S5 Systems
• Optimized Oncomine informatics

Multiple configurations
The Oncomine Comprehensive Assay v3 comes in the following configurations to match your laboratory needs:

Manual library preparation configuration:
Oncomine Comprehensive Library Assay v3M
This configuration contains all the reagents needed to prepare libraries for 24 or 96 samples:
    • Oncomine Comprehensive Panel v3M DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3M RNA—contains the RNA primer pools
    • Ampliseq Library Plus reagents—allow you to manually create your library
Ion Chef automated library preparation configuration:
Oncomine Comprehensive Library Assay v3C
This configuration contains all the reagents needed to prepare libraries for 32 samples:
    • Oncomine Comprehensive Panel v3C DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3C RNA—contains the RNA primer pools
    • Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System


AgriSeq™ HTS Library Kit (Applied Biosystems™)

The Applied Biosystems AgriSeq HTS Library Kit is specifically designed for high-throughput preparation of amplicon libraries for targeted genotyping-by-sequencing (GBS) applications in agrigenomics.

Key product features include:
• Exceptional uniformity of multiplexed target amplification generates robust, reproducible results
• Simplified workflow enables high-throughput sample processing
• Convenient configuration contains library normalization reagents and volumes sufficient for automated platforms

Excellent data quality
AgriSeq library preparation has been optimized for targeted GBS of panels ranging from 100–5000 amplicons. With a highly efficient and specific multiplex technology, AgriSeq amplification can generate libraries with high uniformity (87.2–99.3%), percent on-target reads (85.7–99.7%), and overall genotype call rates (87.5–99.7%) based on a set of nine recently designed panels ranging in size from 62–3000 amplicons across seven different species. The figure below shows an average of 98.2% uniformity for a 3000-amplicon panel across 192 samples (i.e., 98% of bases are covered by at least 0.2X of the average read depth). Even coverage is significant in that it reduces the amount of sequencing required to achieve consistent genotype calls, thus reducing the sequencing cost per sample. In comparison, weaker multiplexing performance is reported with the competitor technology TruSeq, which provides only 88% average uniformity and a reported 1536 maximum multiplexing capability (see reference).

High throughput sample processing
The AgriSeq Library workflow (see figure below) can be performed in either 96- or 384-well plates for high-throughput sample processing using as little as 10 ng of DNA input per reaction. The workflow has been optimized to minimize sample handling and ancillary reagent usage through the processing of pooled samples. This allows the workflow to be completed with minimal pipetting steps, helping to reduce the potential for error, as well as simplifying automation setup on common third party platforms such as Tecan or Hamilton systems. Standard data analysis processing includes automated quality assessment, alignment, and genotype calling using the included Torrent Suite software and Variant Caller plug-in without the need for a bioinformatician, expensive data storage, or third party programs.

Conveniently packaged containing library normalization reagents & volumes for automation
The AgriSeq HTS Kit is offered in large pack sizes (960 or 9600 reactions) to accommodate high throughput agrigenomics projects while minimizing the cost and waste of unnecessary packaging. The AgriSeq HTS Kit includes not only the reagents for library preparation, but all of the components required for library normalization to help achieve consistent results between samples, and sufficient overage has been provided to accommodate automated workflows. With a low price per sample exclusively available for agricultural customers, AgriSeq targeted GBS is an economical way to generate high-throughput genotyping data to advance your breeding programs.

Oncomine™ BCR IGH LR Assay, RNA (Ion Torrent™)

The Oncomine BCR IGH LR Assay is a robust, targeted next-generation sequencing (NGS) assay designed for use in immuno-oncology, infectious disease, and basic immunology research. Unlike other technologies, the Oncomine BCR IGH-LR Assay offers more than 400 base amplicons of library sequencing through long-read sequencing chemistry, enabling comprehensive coverage of the B cell receptor (BCR) immunoglobin heavy (IGH) chain. The assay kit includes a single pool of multiplex PCR primers, cDNA synthesis kit, and AmpliSeq library reagents.

The Oncomine BCR IGH LR Assay is designed to accurately measure clonal expansion, quantify somatic hypermutation, and isotype B lymphocytes using total RNA extracted from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), or sorted cells in fresh frozen (FF) research specimens. The assay utilizes Ion AmpliSeq multiplex PCR technology. It amplifies framework 1 (FR1) and constant gene region of BCR IGH gene to interrogate highly diverse complementarity-determining regions CDR1, CDR2, and CDR3, as well as the CH1 domain of the constant gene to enable distinction of all nine isotypes. IGH CDR-region amino acid motifs may reveal signatures of B cell responses to defined antigens for use as future markers of autoimmune or infectious disease. Automated clonal lineage analysis and multi-sample analysis capability facilitates tracking of B cell clonal evolution arising from somatic hypermutation and class switch recombination in research samples.

Oncomine BCR IGH LR Assay benefits include:
• Extended amplicon allows for quantification of variable gene somatic hypermutation and isotype identification to enable immuno-oncology, and infectious disease translational and clinical research
• Assay primers are designed to amplify all variable and joining gene alleles in the gold-standard IMGT database
• RNA input improves detection of changes in plasmablast and plasma cell populations in research samples following immune challenge or administration of immunomodulatory agents
• Superior multiplex PCR design through AmpliSeq technology assures high accuracy and sensitivity through a superior multiplex PCR design
• Ultra-low substitution error rate minimizes artifacts arising from sequencing errors to enable highly accurate assessment of somatic hypermutation and clonal heterogeneity in malignant B cell research samples of interest
• The entire workflow from isolation of RNA to analysis of samples can be accomplished within two days using the Ion Chef templating system and the Ion Genestudio S5 sequencing systems
• Flexible input requirements ensures successful library construction with as low as 25 ng and up to 2 µg total RNA input
• Streamlined and user-friendly informatics solution offers automated clonotyping, somatic hypermutation quantification, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to track oB cell clones across research samples

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide below.

Ion Library Equalizer™ Kit (Ion Torrent™)

The Ion Library Equalizer™ Kit is a simple and seamless bead-based solution replacing the need for library quantification and library dilutions for library normalization as required for any next generation sequencing workflow. The Ion Library Equalizer™ Kit helps reduce labor, reagent costs, and further simplifies the Ion semiconductor sequencing workflow for high sample-throughput or barcoded projects, as well as single or low sample number projects.

The kit workflow consists of three simple steps:

1) Library amplification with Equalizer™ Primers
2) Library capture onto Equalizer™ Beads
3) Heat elution of the normalized library using a specially formulated Equalizer™ Elution Buffer

Simple Workflow
• Seamlessly integrates into your existing library preparation workflow
• Reduced pipetting compared to the Agilent 2100 Bioanalyzer®-based workflow
• Faster workflow in high-throughput applications-no need for DNA quantification, calculations, and dilution of DNA libraries
• Minimizes library-to-library variability

Cost Effective
• No additional capital equipment required
• Less expensive than Agilent 2100 Bioanalyzer®-based workflow (up to 10 samples)
• Reduced AMPure® XP reagent usage compared to Agilent 2100 Bioanalyzer®-based workflow
• Long shelf life: up to 18 months
• Convenient 96-reaction kit helps reduce ordering frequency

Flexible
• Compatible with all Ion DNA library kits including Ion AmpliSeq™ DNA panels, Ion fragment library kits, and Ion Xpress™ Barcode adaptors
• Use with a single sample or in a 96-well plate format
• Compatible with 3rd party automated liquid handling systems for integrated library preparation and normalization

Includes:
The Ion Library Equalizer Kit includes the necessary reagents to complete 96 normalization reactions.

Required for Workflow:
Ion AmpliSeq™ Library Kit 2.0
Ion AmpliSeq™ Ready-to-Use Panel or Custom Panel designed via the Ion AmpliSeq™ Designer (www.ampliseq.com)
--OR--
Ion Plus Fragment Library Kit
--OR--
Ion Xpress™ Plus Fragment Library Kit

Optional:
Ion Xpress™ Barcode Adapters Kits