Shop All Library Preparation Kits for Sequencing

Oncomine™ Tumor Mutation Load Assay, manual library preparation (Ion Torrent™)

The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutation load and mutation signatures in a simple workflow. The assay provides reagents for library construction from two pools of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) tumor samples without the need for a matched normal sample, using 10 ng of DNA per primer pool. This assay is designed to facilitate successful selection and identification of samples most likely to derive responses in cancer immunotherapy research.

Features:
• Enables accurate quantitation of somatic mutations to assess tumor mutation load
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems

The Oncomine Tumor Mutation Load Assay, Manual Library Preparation, includes panel primers and reagents for library construction sufficient for 24 samples. The libraries are compatible with automated template preparation and chip loading on the Ion Chef System. For automated library preparation using the Ion Chef System, please see Cat. No. A37910.

From sample to answer
The Oncomine Tumor Mutation Load Assay comes with optimized informatics and visualization software that provides a sample-to-answer solution. Run metrics can be assessed using a Torrent Suite plugin. The Ion Reporter analysis workflow utilizes a custom variant calling and germline variant filtering algorithm to accurately estimate somatic variants in cancer research samples. A detailed report is provided that includes the normalized mutation load (mutations/MB) and mutation signatures of the somatic variants, including percentage of mutations consistent with UV damage, tobacco smoke damage, de-amination, and specific substitutions.

Learn more about Oncomine Oncology ›

Ion AmpliSeq™ Kit for Chef DL8 (Ion Torrent™)

The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of Ion AmpliSeq library construction. The kit offers:

• Simplest workflow for targeted sequencing library preparation using 1 or 2 pool Ion AmpliSeq panel designs*
• Low input
• Easy-to-use cartridge format with reagent barcoding for full reagent tracking
• Proven performance of Ion AmpliSeq technology, compatible with DNA extracted from formalin-fixed, paraffin-embedded tissue and other challenging sources

*See www.AmpliSeq.com for custom 1-2 pool panels. Note that some panels (e.g., Transcriptome, Cancer Hotspot, Oncomine BRCA, Pharmacogenomics) have been prepared in an Ion Chef-ready kit by incorporating the respective ready-made primer pool with the DL8 kit.

Leveraging the automation capabilities of the Ion Chef System, the Ion AmpliSeq Kit for Chef DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps. Compatible with 1- and 2-pool Ion AmpliSeq panel designs, the kit automates the preparation of libraries from 8 samples in a single run, delivering a single tube of pooled libraries ready for downstream templating and sequencing. The kit provides sufficient material for the preparation of 32 libraries (4 Ion Chef runs).

Utilizing plug and play, pre-packaged, single-use cartridges and PCR plates for a fully automated workflow, users of all experience levels will find library preparation straightforward with minimal effort. Supplied with barcodes conveniently dried-down in 96-well plates, multiplexing samples is easy and requires no additional pipetting steps.

More information on Ion AmpliSeq technology >

Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems (Applied Biosystems™)

Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems is a reagent component of the AB Library Builder™ System. This kit, along with separately purchased Agencourt® AMPure® Beads, allows for the creation of up to 13 RNA whole transcriptome libraries on the Library Builder™.
• Automation – reduce hands on time by 50% or more depending on the number of libraries
• Scalable – prepare up to 13 libraries per run, up to 26 libraries per day
• Integrated – compatible with the SOLiD® 4 System and 5500 Series Genetic Analysis Systems

Labor Saving Automation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems when used with the AB Library Builder™ System automates the most labor-intensive parts of RNA whole transcriptome library preparation, helping to reduce hands-on time by at least 50%. The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems contains enough reagents and materials to process up to 13 samples at a time.

Integrated RNA Library Preparation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems, along with the AB Library Builder™ System, is an integrated solution comprising a predefined software protocol, plug-and-play cartridge format reagents, and instrument. These kits are all optimized and validated for RNA purification and RNA whole transcriptome library preparation with the 5500 and SOLiD® 4.

To further maximize automation consider using RNA purification protocols with the iPrep™ PureLink® Total RNA Kit, compatible with the Library Builder™ System.

For Research Use Only. Not for use in diagnostics procedures.

Precision ID mtDNA Whole Genome Panel (Applied Biosystems™)

The Applied Biosystems Precision ID mtDNA Whole Genome Panel is an innovative next-generation sequencing (NGS) approach to mitochondrial DNA (mtDNA) analysis specifically developed for forensic applications. It is a 2-pool multiplex assay that targets the entire human mitochondrial genome (16,569 bp) (see figure below). Each pool contains 81 primer pairs, with minimal primer overlap between pools. This panel was constructed using a innovative tiling approach to obtain optimal coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones that uses massively parallel sequencing built on Ion AmpliSeq technology. This mtDNA tiling approach was also used to construct the Precision ID mtDNA Control Region Panel (Cat. No. A31443) which targets only the control region of the genome.

Simplicity
• Small amplicons enable better recovery of mtDNA genome with degraded samples
• As little as 2 pg of input
• Automated library and template preparation using the Ion Chef System
• Optimized analysis using Converge 2.1 software

Scalability
• Multiple chip formats to meet a range of sample throughput requirements
• Ability to multiplex up to 32 samples*

Speed
• Less than 45 minutes of hands-on time for a DNA-to-data targeted sequencing workflow*
• Total sample-to-data time of less than 2 days*

Mitochondrial DNA analysis is a key DNA detection method used by forensic scientists when samples such as teeth, bones, and hairs without a follicle (root) are collected from crime scenes, missing person remains, or disaster areas that have been exposed to high environmental stress where nuclear DNA is insufficient in quality and/or quantity. Balanced coverage is achieved using a small amplicon design (163 bp average amplicon length), optimized with the inclusion of degenerate primers that align with primer-binding SNPs in the Precision ID mtDNA Whole Genome Panel, which increases success with degraded and low template DNA samples. This panel can be used with all types of forensic samples, including ancient DNA remains as well as clinical research specimens.

Additionally, full automation of library and template preparation of the Precision ID mtDNA Whole Genome Panel using the Ion Chef System and sequencing on the Ion GeneStudio series systems can reduce variation observed with manual workflows (see figure below) and streamline user-instrument interaction with less than 45 minutes of hands-on time. The Converge NGS Analysis module automates mtDNA analysis, leveraging optimized base calling, phylogenetically-guided alignment, and quality filtering algorithms specific for the Precision ID mtDNA panels. Variant calling including, including heteroplasmy detection can be easily optimized within the software features. These solutions give forensic DNA laboratories the flexibility to detect variation within noncoding control sequences using the Precision ID mtDNA Control Region Panel or take advantage of the genetic diversity of full mtGenome sequence data using the Precision ID mtDNA Whole Genome Panel.

NOTE: The Precision ID mtDNA Whole Genome Panel provides sufficient material to perform 96 reactions using the manual Precision ID Library kit (Cat. No. A26435) or 48 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212).

*Using the Precision ID NGS System for Human Identification

Oncomine™ Tumor Mutation Load Assay, Chef-ready library preparation (Ion Torrent™)

The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutation load and mutation signatures in a simple workflow. The assay provides reagents for library construction from two pools of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) tumor samples without the need for a matched normal sample, using 10 ng of DNA per primer pool. This assay is designed to facilitate successful selection and identification of samples most likely to derive responses in cancer immunotherapy research.

Features:
• Enables accurate quantitation of somatic mutations to assess tumor mutation load
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems

The Oncomine Tumor Mutation Load Assay, Chef-Ready Library Preparation, includes panel primers and reagents for automated library construction and normalization sufficient for 32 samples. This version of the assay is for automated library preparation using the Ion Chef System. For manual library preparation, please see Cat. No. A37909.

From sample to answer
The Oncomine Tumor Mutation Load Assay comes with optimized informatics and visualization software that provides a sample-to-answer solution. Run metrics can be assessed using a Torrent Suite plugin. The Ion Reporter analysis workflow utilizes a custom variant calling and germline variant filtering algorithm to accurately estimate somatic variants in cancer research samples. A detailed report is provided that includes the normalized mutation load (mutations/MB) and mutation signatures of the somatic variants, including percentage of mutations consistent with UV damage, tobacco smoke damage, de-amination, and specific substitutions.

Learn more about Oncomine Oncology ›

Oncomine™ Comprehensive Library Assay (Ion Torrent™)

The Oncomine™ Comprehensive Library Assay is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 143 unique genes to help inform drug discovery research, and clinical trial research programs. It provides the reagents for library construction and three pools—two DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per pool, the assay covers more than 2,500 amplicons and 170 biomarkers.

The Oncomine Comprehensive Library Assay includes oligos for library construction. For a kit that also includes reagents and consumables for next-generation sequencing, please see the Oncomine Comprehensive Assay.

• Enables analysis of variants across 143 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool, isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and >300 FFPE samples
• NGS results concordant with verified single gene tests [1]
• Additional relevant variants detected
• Content driven by the Oncomine™ Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published clinical trials

From sample to answer
The Oncomine Comprehensive Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds, to just a few key cancer driver variants. Enabled by Oncomine™ informatics, this filtering, and as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Comprehensive Assays >

1. Hovelson et al., 2015. Development and validation of a scalable next-generation sequencing system for assessing relevant somatic variants in solid tumors. Neoplasia 17:385-399.

Precision ID GlobalFiler™ NGS STR Panel (Applied Biosystems™)

The Applied Biosystems™ Precision ID GlobalFiler™ NGS STR Panel enables an innovative next-generation sequencing (NGS) approach to analysis of DNA mixtures for casework applications using massively parallel sequencing built on Ion AmpliSeq™ technology for superior efficiency. It includes the same 21 autosomal STRs, along with a y-indel and two Amelogenin sex markers, found in the GlobalFiler™ PCR Amplification Kit (Cat. Nos. 4476135 and 4482815) (see figure below). Instead of including the SE-33 marker found in the kit, this panel includes 9 additional multi-allelic STR markers (for a total of 33 targets, of which three are male-specific) to aid in mixture interpretation for complex casework samples. Using minimal input of 1 ng of DNA, this targeted forensic marker panel enables a sample extraction to genotypes workflow using the Ion PGM™ System and HID STR Genotyper Analysis plug-in.

Simplicity
● As little as 1 ng of input DNA
● Manual library preparation (automated Ion Chef™ library preparation coming soon)
● Template preparation with the One Touch™ 2 System and Ion Chef™ System
● Interpret profiles with easy-to-use HID STR Genotyper plug-in

Scalability
● Multiple Ion PGM chip formats to meet sample throughput requirements (Ion S5™ chip options coming soon)
● Ability to multiplex up to 64 samples for efficient sample processing

The Precision ID Globalfiler NGS STR Panel provides both allele number and base sequence for each repeat structure, offering enhanced mixture resolution, and an increase in Probability of Identity (PI) estimates. This unique NGS STR panel combines maximum compatibility with global expanded CODIS core loci databasing standards and superior discrimination power, enabling forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The Precision ID Globalfiler NGS STR Panel is optimized for casework samples.

Oncomine™ Focus Assay, Select Library (Ion Torrent™)

The Oncomine Focus Assay, Select Library, is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables the detection of variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. The assay provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single sequencing run.

The Oncomine Focus Assay, Select Library, includes panel primers and reagents for library construction and normalization. The Select Library reagents, manufactured to rigorous standards in our ISO 13485-compliant facility, are sufficient for 48 samples, including 16 barcodes, facilitating the combination of up to 8 samples of DNA and RNA in a single run. The libraries are compatible with automated template preparation and chip loading on the Ion Chef System. For a kit that also includes reagents and consumables for next-generation sequencing on the Ion PGM System using the Ion OneTouch 2 System, please see the Oncomine Focus Assay, 318 Solution.

From sample to answer
The Oncomine Focus Assay, Select Library, is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Focus Assays and Oncomine Oncology.

Oncomine™ TCR Beta-LR Assay (Ion Torrent™)

The Oncomine TCR Beta-LR Assay is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements. This assay utilizes a newly developed long-read sequencing technology on the Ion S5 and Ion S5 XL platforms. The assay kit provides a single pool of multiplex PCR primers, library reagents, and sample barcodes. Ion AmpliSeq libraries can be generated from research samples such as RNA extracted from whole blood, fresh-frozen tissue, or FACS-sorted cells.

Learn more about the assay ›

The assay is designed to efficiently capture all three complementarity determining regions of the TCR beta chain (CDR1, CDR2, CDR3) with high accuracy. It enables key applications such as predictive or prognostic biomarker discovery, T cell characterization, and identification of variable gene polymorphisms. The identification of rare and abundant clones can be achieved with a flexible RNA input amount of 10 ng (minimum) and up to 1 µg. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—improving the identification of rare clones.

Note: Information about data analysis using Ion Reporter Software v5.6 can be found in the User Guide below.

Benefits of the Oncomine TCR Beta-LR Assay include:
• Long-read RNA sequencing (up to 400 bp amplicon length) comprehensively covering TCR beta chain
• Detection of clones over a wide dynamic range
• Unbiased output generated from Ion AmpliSeq technology
• Efficient workflow with 48 hr sample-to-results time
• Flexible input requirements ranging from 10 ng to 1 µg
• Compatibility with a variety of research sample types including fresh-frozen tissue, whole blood, and sorted cells
• Streamlined and user-friendly informatics solution with multi-sample analysis functionality

Why are immunologists and immuno-oncologists interested in T cell repertoire sequencing research?
• To characterize diversity and monitor features of T lymphocytes in blood and infiltrated tumors
• To identify variable gene polymorphisms implicated in autoimmune disease or immune-mediated adverse events
• To optimize the manufacture and function of therapeutic or engineered T cells

Unlike other technologies, the Oncomine TCR Beta-LR Assay offers:
• Long-read sequencing, enabling comprehensive coverage of TCR beta chain
• High accuracy and sensitivity through a superior multiplex PCR design
• Use of very low input RNA as a starting material (10 ng)
• High multiplexing and low cost per sample, enabling large-scale studies
• Accelerated discovery cycles through fast turnaround time

The entire workflow (figure below) from isolation of RNA to analysis of samples can be accomplished in two days using the Ion Chef templating system and the Ion S5 or Ion S5 XL sequencing system.

Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (Ion Torrent™)

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit enables the simultaneous measurement of the expression levels of over 20,000 human RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA derived from challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue or other samples with degraded RNA, the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit is ideal for those interested in obtaining comprehensive, highly sensitive, and easily obtained gene expression measurements from a wide range of RNA sources.

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit offers:
• Flexible, straightforward library preparation – start with as little as 10 ng of total RNA from FFPE samples, cells, or other sources
• Representation of over 20,000 human RefSeq genes
• Wider dynamic range and improved detection sensitivity compared to conventional microarray analysis
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting as well as quality control metrics
• Compatibility with Ion Xpress™ Barcode adapters allowing for multiplexed sequencing

Wider dynamic range for enhanced performance
With a wider dynamic range and improved gene detection compared to conventional microarray analysis, the Ion AmpliSeq Transcriptome™ Human Gene Expression Kit provides a clear solution to researchers needing enhanced performance for their gene expression needs. A simple workflow allows for sample-to-gene quantitation in less than 2 days, helping to reduce workload and increase throughput.

Straightforward library preparation and multiplex sequencing
Starting with as little as 10 ng of total RNA, libraries can be prepared in approximately 7 hours with only one hour of hands-on time. Total RNA is first processed with the included SuperScript® VILO™ cDNA Synthesis kit, and the resulting cDNA is amplified using Ion AmpliSeq™ technology to accurately maintain expression levels of all genes.

The resulting libraries are ready for downstream template preparation using the Ion OneTouch™ 2 System or Ion Chef™ System and sequencing on the Ion Proton™ sequencing system. Libraries can be barcoded for multiplexing, allowing for flexibility, helping to reduce cost, and increase throughput, with the recommended 8 libraries per Ion PI™ chip. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Automatic data analysis with Torrent Suite™ plug-in
Using the complementary analysis plug-in available with Torrent Suite™ software, gene quantitation information is automatically compiled, minimizing the requirement for costly computational resources and bioinformatics expertise to process raw sequencing data into meaningful information. An easy to interpret, highly portable table is produced containing all genes interrogated and their respective expression levels, output that may be used with a variety of existing software applications for downstream analysis of microarray or next-generation sequencing data.

Note: Libraries made from this protocol yield fragments suitable for 200-base read sequencing.

Precision ID Library Kit (Ion Torrent™)

The Precision ID Library Kit is designed for rapid preparation of amplicon libraries using HID-Ion AmpliSeq ready-to-use panels, for sequencing on Ion Torrent sequencing system. This kit uses only certain components found in the Ion AmpliSeq™ Library Kit 2.0, that are needed for Precision ID applications, making the Precision ID Library Kit more customized and specific for forensic research use only.

Key features:

Simple protocol
• 96 well plate-based workflow allows easy sample management
• No library amplification with qPCR quantification

Ion AmpliSeq™ HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs

Digestion reagent
• Cleaner digest allows for more efficient downstream sequencing

Benefits for HID applications:

• 1 ng of DNA input is recommended for the Precision ID panels
• Less components to help save time and money by preventing unnecessary waste

Scalable multiplex PCR reactions
The Precision ID Library Kit is built on Ion AmpliSeq chemistry, which enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 1 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets.

Barcoded library preparation
The Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1–96 kits and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and flexible protocol
The Precision ID Library Kit uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories.

The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP or STR Genotyper plug-ins enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.

*Using the Applied Biosystems™ Precision ID NGS System for Human Identification

Ion 520™ Food Protection Chip Kit

Simplify sample tracking and sequencing using this Chip Kit as part of a complete Thermo Scientific™ NGS Workflow for Food Authenticity. 

MuSeek Library Preparation Kit, Ion Torrent compatible (Thermo Scientific™)

Thermo Scientific MuSeek Library Preparation Kit for Ion Torrent™ is designed for generation of high‐quality genomic DNA libraries for sequencing with the Ion Personal Genome Machine™ (PGM™) and Ion Proton™ systems. The fast protocol utilizes MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adaptors are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 100 ng of the sample DNA the same protocol can be used to generate 100-400 bp insert libraries.

Highlights

Fast—generates NGS fragment library in less than 80 minutes
Convenient —no physical shearing, end-repair and ligation steps
Easy to use—same protocol for generating different length DNA fragment libraries

Applications

• DNA fragment library preparation for downstream use in next generation sequencing workflow.

Includes
• MuSeek Enzyme Mix
• MuSeek Fragmentation Reaction Buffer
• MuSeek Stop Solution
• Control DNA
• MuSeek Adaptor Addition Primer Mix
• MuSeek Adaptor Addition Reaction Buffer
• Phusion Hot Start II High-Fidelity DNA Polymerase
• MuSeek Sequencing Primer

Note: The kit is shipped on dry ice. Upon arrival, open the kit and place MuSeek Enzyme Mix for storage below -70°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature, and the remaining kit components at -20°C.

SGS™ All Species ID Food DNA Analyser Kits (Thermo Scientific™)

Identify meat, plant or fish species present in all food sample types with ready-to-use DNA test kits, software and Ion Torrent NGS technology.

Oncomine™ Focus Assay, AmpliSeq™ Library (Ion Torrent™)

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables the detection of variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. The assay provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single sequencing run.

The Oncomine Focus Assay, AmpliSeq Library, is for use as part of the Ion Chef and Ion S5 Systems workflow. Scalability and flexibility are achieved using Ion Xpress or IonCode barcode adapters for multiplexing barcoded samples on Ion S5 chips. This version of the assay includes panel primers and AmpliSeq reagents for library construction. For a kit that includes barcodes and Select Library reagents, please see the Oncomine Focus Assay, Select Library. For a kit that also includes reagents and consumables for next-generation sequencing on the Ion PGM System, please see the Oncomine Focus Assay, 318 Solution.

From sample to answer
The Oncomine Focus Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Focus Assay and Oncomine oncology.