Shop All Library Preparation Kits for Sequencing

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detectio
• >98% strand specificity
• Efficient detection of non-coding RNA

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~150 bp for mRNA or whole transcriptome libraries with sample inputs of 1–25 ng of mRNA-enriched or ribo-depleted RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based library purification steps, enable completion of the entire workflow in approximately 4.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The Collibri Stranded RNA Library Prep Kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit is recommended for qPCR-based quantification of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Gene fusion detection
• Detection of transcript isoforms

AgriSeq™ HTS Library Kit (Applied Biosystems™)

The Applied Biosystems AgriSeq HTS Library Kit is specifically designed for high-throughput preparation of amplicon libraries for targeted genotyping-by-sequencing (GBS) applications in agrigenomics.

Key product features include:
• Exceptional uniformity of multiplexed target amplification generates robust, reproducible results
• Simplified workflow enables high-throughput sample processing
• Convenient configuration contains library normalization reagents and volumes sufficient for automated platforms

Excellent data quality
AgriSeq library preparation has been optimized for targeted GBS of panels ranging from 100–5000 amplicons. With a highly efficient and specific multiplex technology, AgriSeq amplification can generate libraries with high uniformity (87.2–99.3%), percent on-target reads (85.7–99.7%), and overall genotype call rates (87.5–99.7%) based on a set of nine recently designed panels ranging in size from 62–3000 amplicons across seven different species. The figure below shows an average of 98.2% uniformity for a 3000-amplicon panel across 192 samples (i.e., 98% of bases are covered by at least 0.2X of the average read depth). Even coverage is significant in that it reduces the amount of sequencing required to achieve consistent genotype calls, thus reducing the sequencing cost per sample. In comparison, weaker multiplexing performance is reported with the competitor technology TruSeq, which provides only 88% average uniformity and a reported 1536 maximum multiplexing capability (see reference).

High throughput sample processing
The AgriSeq Library workflow (see figure below) can be performed in either 96- or 384-well plates for high-throughput sample processing using as little as 10 ng of DNA input per reaction. The workflow has been optimized to minimize sample handling and ancillary reagent usage through the processing of pooled samples. This allows the workflow to be completed with minimal pipetting steps, helping to reduce the potential for error, as well as simplifying automation setup on common third party platforms such as Tecan or Hamilton systems. Standard data analysis processing includes automated quality assessment, alignment, and genotype calling using the included Torrent Suite software and Variant Caller plug-in without the need for a bioinformatician, expensive data storage, or third party programs.

Conveniently packaged containing library normalization reagents & volumes for automation
The AgriSeq HTS Kit is offered in large pack sizes (960 or 9600 reactions) to accommodate high throughput agrigenomics projects while minimizing the cost and waste of unnecessary packaging. The AgriSeq HTS Kit includes not only the reagents for library preparation, but all of the components required for library normalization to help achieve consistent results between samples, and sufficient overage has been provided to accommodate automated workflows. With a low price per sample exclusively available for agricultural customers, AgriSeq targeted GBS is an economical way to generate high-throughput genotyping data to advance your breeding programs.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with UD indexes (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow—total RNA converted to sequencing-ready library within 4.5 hours
• Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA

This Collibri Stranded RNA Library Prep Kit supports fast library preparation within 4.5 hours for mRNA or whole transcriptome libraries with 1–25 ng of mRNA-enriched or ribo depleted RNA sample input. Multiplexing of libraries can be carried out using up to 96 single-indexed or unique dual-indexed primers, enabling single-read or paired-end sequencing. The kit is suitable for various-quality RNA samples, including FFPE samples.

Visual cues of process integrity
For maximum convenience, visual cues of process integrity are included throughout the library preparation workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

How it works
Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed or unique dual-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 either single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer or unique dual-indexed PCR primers that enable multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit, is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems with Human/Mouse/Rat rRNA Depletion Kit (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. Total RNA can be converted to sequencing-ready library within 6.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~ 150 bp for whole transcriptome libraries starting with sample inputs of 100–500 ng of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related prioduct, the Collibri Library Quantification Kit is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Genexus™ Barcodes 1-96 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 1-96 AS kit provides one each of Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 65-96 AS. When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, it supports flexible, fully automated preparation and normalization of up to 96 Ion AmpliSeq libraries. Each plate is filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

SGS™ All Species ID Food DNA Analyser Kits (Thermo Scientific™)

Identify meat, plant or fish species present in all food sample types with ready-to-use DNA test kits, software and Ion Torrent NGS technology.

Ion Torrent™ Dual Barcode Kit 1-96 (Ion Torrent™)

The Ion Torrent Dual Barcode Kit 1-96 provides 96 unique dual-matched barcode adapters specifically designed to enable optimal performance of amplicon libraries and Ion semiconductor sequencers. When used in combination with Ion AmpliSeq and Oncomine assays, the kit enables users to pool up to 96 amplicon libraries to conduct multiplexed sequencing analysis, thereby increasing lab efficiency and reducing sequencing costs per sample. The barcode adapters are provided in 96-well plate format.

Features of the Ion Torrent Dual Barcode Kit 1-96 include:
• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes to identify very rare clones with high accuracy
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Higher throughput and variant calling of rare clones
Multiplexing with dual-barcoded libraries enables cost-effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias and provides superior sensitivity in detecting low-frequency clones with high accuracy.

Sequence- and flow-optimized for increased performance and efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require a minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design
Built-in error correction increases the accuracy of sample assignment.

Ion Ampliseq™ Cancer Hotspot Panel v2 Chef-Ready Kit (Ion Torrent™)

The Ion Ampliseq™ Cancer Hotspot Panel v2 Chef-Ready Kit combines a single pool of primers with the reagents necessary to prepare libraries of frequently mutated regions in human cancer genes on the Ion Chef™ System. This automated workflow utilizes the same chemistry as the manual library construction workflow. It provides equivalent or better performance with exceptional reproducibility, on-target reads, and sample balance. Automated production of libraries using the Ion Chef System reduces hands-on time to less than 15 minutes.

The Ion Ampliseq Cancer Hostpot Panel v2 Chef Ready Kit consists of the following components:
• Ion Ampliseq™ Cancer Hotspot Research Panel Primers
• Ion Ampliseq™ Kit for Chef DL8

The simple sample-to-data workflow can be executed in less than two days and offers the following features and benefits:
• Production of high-quality DNA libraries using the Ion Chef System
• Proven performance of Ion Ampliseq™ technology, FFPE compatibility, uniform coverage for efficient sequencing, accurate variant calling, and improved variant detection via Torrent Suite™ Software
• Ability to scale and multiplex on Ion™ chips based on desired throughput needs, driving cost savings and efficiency
• Simplified workflow and reduced hands-on time when used with the Ion Ampliseq™ Direct FFPE DNA Kit
• Low input amount of only 1 ng per sample

Additional mutational content for advancement of cancer research studies
The Ion AmpliSeq Cancer Hotspot Panel v2 is designed to amplify 207 amplicons covering approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes. The panel should be used with the Ion AmpliSeq™ Library Kit 2.0.

Minimal hands-on time
Leveraging the automation capabilities of the Ion Chef System, the included Ion AmpliSeq Kit for Chef Kit DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps. Compatible with 1- and 2-pool Ion AmpliSeq panel designs, the kit automates the preparation of libraries from 8 samples in a single run, delivering a single tube of pooled libraries ready for downstream templating and sequencing. The kit provides sufficient material for the preparation of 32 libraries.

Simplicity and ease-of-use of Ion solutions
Utilizing plug and play, pre-packaged, single-use cartridges and PCR plates for a fully automated workflow, users of all experience levels will find library preparation straightforward with minimal effort. Supplied with barcodes conveniently dried-down in 96-well plates, multiplexing samples is easy and requires no additional pipetting steps.

Oncomine™ Childhood Cancer Research Assay (Ion Torrent™)

The Ion Torrent Oncomine Childhood Cancer Research Assay consists of four pools of Ion AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on the Ion GeneStudio S5 platforms. The assay is designed to provide researchers with sensitive and comprehensive sample amplification of relevant DNA mutations and fusion transcripts associated with childhood and young adult cancers in a single NGS run. The panel is comprised of 202 unique genes: 130 key DNA genes, 28 copy number variant targets, an expansive fusion panel of 90 driver genes with multiple partners, and 9 expression genes and controls. These cover the most relevant targets in the vast majority of all childhood and young adult oncology research samples.

This version of the assay is for manual library preparation. For automated library preparation using the Ion Chef System, see the Oncomine Childhood Cancer Research Assay, Chef-Ready.

Features include:
• Simple, robust workflow that encompasses mutations and fusions in two DNA and two RNA pools in a single NGS run
• Excellent coverage of all relevant targets
• Multiplex up to eight samples on a single Ion 540 Chip
• Compatible with as little as 10 ng/pool input DNA or RNA per library from blood, bone marrow, or FFPE samples
• Validated detection of somatic variants down to 5% allele frequency
• Simple and fast workflow produces targeted libraries in 3.5 hours typically
• For use on the Ion GeneStudio S5 Systems with manual or automated library preparation

The Oncomine Childhood Cancer Research Assay is a comprehensive, targeted NGS assay designed to assist researchers in the understanding of childhood and young adult cancer. The assay is a complete kit facilitating the amplification of multiple gene targets from FFPE, blood, or bone marrow samples with as little as 10 ng of input DNA or RNA per pool.

Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on the Ion GeneStudio S5 sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatics workflows within Torrent Suite and Ion Reporter analysis software that utilize optimized variant calling parameters for SNV, InDel, copy number variant, and fusion transcript detection. Samples can be processed quickly and easily, and variants detected and identified confidently.

Ion PI™ Chip Kit v3 (Ion Torrent™)

The Ion PI™ Chip Kit v3 contains 8 barcoded chips for sample tracking and sequencing with the Ion Proton™ System. The Ion PI™ Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this revolutionary advance in next-generation sequencing technology allows for rapid sequencing times of less than 4 hours. The Ion PI™ Chip v3 enables a simplified sequencing workflow with the removal of chip preparation steps, reducing the hands-on time by approximately 45 minutes compared to previous versions.

The Ion PI™ Chip Kit v3 offers:
• A simplified workflow, reducing hands-on time by approximately 45 minutes
• Barcoded sequencing chip with sample tracking functionality
• 10 Gb of sequence data with a rapid run time of typically only 2-4 hours
• Support for a variety of applications, including sequencing of up to three human exomes, transcriptome profiling with 60-80 million reads, and targeted resequencing of gene panels

Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q OT2 200 Kit and Ion PI™ Hi-Q Sequencing 200 Kit.
This kit replaces the Ion PI™ Chip Kit v2 & Ion PI™ Chip Kit v2 BC.

Genexus™ Barcodes 1-32 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 1-32 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 33-64 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-32 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with human/mouse/rat rRNA depletion kit and UD indexes (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow— total RNA can be converted to sequencing-ready library within 6.5 hours
• Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

This Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation starting with sample inputs of 100 ng—1 µg of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various-quality RNA samples, including FFPE samples.

Visual cues of process integrity
For maximum convenience, visual cues of process integrity are included throughout the library preparation workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

How it works
The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed or unique dual-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 either single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer or unique dual-indexed PCR primers that enable multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit, is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Oncomine™ Childhood Cancer Research Assay, Chef-Ready (Ion Torrent™)

The Ion Torrent Oncomine Childhood Cancer Research Assay consists of four pools of Ion AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on the Ion GeneStudio S5 platforms. The assay is designed to provide researchers with sensitive and comprehensive sample amplification of relevant DNA mutations and fusion transcripts associated with childhood and young adult cancers in a single NGS run. The panel is comprised of targets from 202 unique genes: 130 key DNA genes, 28 copy number variant targets, an expansive fusion panel of 90 driver genes with multiple partners, and 9 expression genes and controls. These cover the most relevant targets in the vast majority of all childhood and young adult oncology research samples.

This version of the assay is for automated library preparation using the Ion Chef System. For manual library preparation, see Cat. No. A36485.

Features include:
• Simple, robust workflow that encompasses mutations and fusions in two DNA and two RNA pools in a single NGS run
• Excellent coverage of all relevant targets
• Multiplex up to eight samples on a single Ion 540 Chip
• Compatible with as little as 10 ng/pool input DNA or RNA per library from blood, bone marrow or FFPE samples
• Validated detection of somatic variants down to 5% allele frequency
• Simple and fast workflow produces targeted libraries in 3.5 hours typically
• For use on the Ion GeneStudio S5 Systems with manual or automated library preparation

The Oncomine Childhood Cancer Research Assay is a comprehensive, targeted NGS assay designed to assist researchers in the understanding of childhood and young adult cancer. The assay is a complete kit facilitating the amplification of multiple gene targets from FFPE, blood, or bone marrow samples with as little as 10 ng of input DNA or RNA per pool.

Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on the Ion GeneStudio S5 sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatics workflows within Torrent Suite and Ion Reporter analysis software that utilize optimized variant calling parameters for SNV, InDel, copy number variant, and fusion transcript detection. Samples can be processed quickly and easily, and variants detected and identified confidently.

Precision ID Identity and Library Kit Bundle (Applied Biosystems™)

The Applied Biosystems™ Precision ID Identity Panel and Library Kit Bundle is packaged for easy ordering and preparation of amplicon libraries using the Precision ID Identity Panel (Cat. No. A25643) and the Precision ID Library Kit (Cat. No. A26435) for sequencing on Ion Torrent sequencing systems. The Precision ID Identity Panel of multiplexed SNPs provides unique identification of degraded or trace samples with discrimination similar to the STR genotype match probabilities used by forensic analysts (between 1x10–31 and 6x10–35).

Simple protocol
• 96 well plate-based workflow enables easy sample management
• No library amplification with qPCR quantification

Optimized digestion reagent
• Cleaner digest allows for more efficient downstream sequencing

Scalable multiplex PCR reactions
The Precision ID Identity Panel is built on Ion AmpliSeq™ chemistry which enables scalable multiplex PCR reactions for all 124 markers in the panel using just 1 ng of starting DNA, with superior coverage uniformity and specificity (acceptable performance has also been observed with sub-nanogram input quantities as low as 180 pg). In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Learn more about other Precision ID panels >

The included Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using the Ion Xpress™ Barcode Adapters 1-96 Kit, and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and flexible protocol
The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP Genotyper plug-in enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.

* Using the Applied Biosystems™ Precision ID NGS System for Human Identification

ClaSeek Library Preparation Kit, Illumina compatible (Thermo Scientific™)

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input.

The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.

Highlights

PCR-free—significantly minimized bias and improved coverage with amplification-free protocol
Flexible—universal protocol for variable sample input (5 ng—1 µg) and DNA fragment lengths
Fast and Convenient—fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure.

Applications

DNA fragment library preparation for downstream use in next generation sequencing workflow.

Related Products
ClaSeek Library Preparation Kit, Illumina compatible

Oncomine™ BRCA Research Assay, Chef-Ready Library Preparation (Ion Torrent™)

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms. The assay is designed to provide sensitive and comprehensive sample amplification of all coding regions of the human BRCA1 and BRCA2 genes. This version of the assay is for automated library preparation using the Ion Chef™ System. For manual library preparation, please see Cat. No. A32840.

Features include:

• Validated for use on Ion 318 and Ion 530 chips
• Detects SNVs, InDels, and large exon/gene deletions/duplications
• Compatible with as little as 10 ng input DNA from formalin-fixed, paraffin-embedded (FFPE) tissue or blood samples
• Highly uniform coverage across all coding exons & splice sites for efficient sequencing and accurate analysis
• Optimized primer design and amplification chemistry enable highly specific target enrichment
• Validated detection of somatic variants to 5% and lower
• Simple and fast workflow produces targeted libraries in 3.5 hours typically

The Oncomine BRCA Research Assay is a complete kit facilitating the amplification of the entire exonic region of both BRCA genes from FFPE tissue or blood samples with as little as 10 ng of input DNA. Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on either the Ion PGM™ or Ion S5™ sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatic workflows within Torrent Suite™ and Ion Reporter™ analysis software that utilize optimized variant calling parameters for SNV, InDel, and large exon/gene deletion/duplication detection. Samples can be processed quickly and easily, and variants detected and identified confidently in either germline or somatic DNA analysis pipelines.

BRCA1 and BRCA2 genes are tumor suppressor genes that code for proteins that are vital components of the homologous recombination pathway of DNA damage repair. BRCA mutations resulting in the deficiency of either gene have been shown to result in inefficient activation of this function and are linked to hereditary predisposition to cancer. Errors in the coding sequence of BRCA genes have been detected in germline and somatic mutations and also occur in tumor samples.

Genexus™ Barcodes 33-64 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 33-64 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 1-32 AS, 65-96 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 33-64 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion AmpliSeq™ Library Kit 2.0 (Ion Torrent™)

The Ion AmpliSeq™ Library Kit 2.0 is designed for rapid preparation of amplicon libraries using Ion AmpliSeq™ panels.

Scalable Multiplex PCR Reactions
The Ion AmpliSeq 2.0 technology enables scalable multiplex PCR reactions from 12- to 24,000-plex in a single well using just 10 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets. Ion AmpliSeq™ Custom Primer Pools are designed via the Ion AmpliSeq™ Designer Tool, available at www.ampliseq.com.

Barcoded Library Preparation
The Ion AmpliSeq Library Kit 2.0 includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1-96 kits or IonCode™ Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and Flexible Protocol
The Ion AmpliSeq Library Kit 2.0 uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The library preparation uses very small quantities of starting material per PCR. The resulting DNA libraries are ready for downstream template preparation using the automated Ion Chef™ System (which also provides the option of automated library preparation using the Ion AmpliSeq™ Kit for Chef DL8) or the Ion OneTouch™ 2 System followed by sequencing. The intuitive Torrent Suite™ Software and optional Ion Reporter™ Software enables automation of data analysis.

Oncomine™ Lung cfDNA Assay (Ion Torrent™)

The Oncomine™ Lung cfDNA Assay is part of a complete research solution to detect lung tumor-derived DNA (ctDNA) in cfDNA. It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cell-free DNA (cfDNA) obtained from the plasma fraction of a single tube of whole blood. The assay enables researchers to analyze single nucleotide variants and short indels that are frequently mutated in non-small cell lung cancer (NSCLC). Eleven genes and >150 hotspots are covered (Genes: ALK, BRAF, EGFR, ERBB2, KRAS, MAP2K1, MET, NRAS, PIK3CA, ROS1, and TP53). Through the use of proprietary sequencing technology, a limit of detection (LOD) down to 0.1% can be achieved.

Note: Target and hotspot BED files and User Guide document for cfDNA Variant Analysis with Torrent Suite Software 5.2 can be found below under "Documents".

• Optimized amplicon design for short cfDNA (~170 bp)
• Detection of both single nucleotide variants (SNVs) and short indels down to 0.1% LOD
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs
• Very high sensitivity and specificity, enabling extremely low levels of detection with minimal false positives
• Efficient workflow, from a single tube of blood to results in just 2 days

Why are pathologists and oncologists interested in liquid biopsy clinical cancer research? Liquid biopsies offer several advantages over conventional solid tumor samples:
• Less invasive to obtain, enabling tumor content to be sampled multiple times
• Lower cost compared to traditional tissue samples
• Faster turnaround time from sample to results
• Help capture more of the heterogeneity of the tumor
• May lead to improvements in standard of care in the future

The entire workflow (figure below) from isolation of cfDNA using the MagMAX™ Cell-Free DNA Isolation Kit to analysis of samples can be accomplished in just 2 days using the Ion Chef™ System and the Ion S5™ XL sequencing system.

Unlike other technologies with low LODs (1-5%), the Oncomine Lung cfDNA Assay has a flexible detection limit down to 0.1% or 1 mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used, but the resulting LOD will be higher depending on the input amount.

Simplicity, speed, and scalability
The Oncomine Lung cfDNA Assay enables cancer genetic studies from as little as 1 ng of input cfDNA for targeted library construction. The cfDNA assay uses standard PCR equipment and two simple PCR reactions, one for sequencing and the second to amplify the library for high multiplex PCR-based target selection, which minimizes the need for extensive capital investment. Additionally, the Oncomine Lung cfDNA Assay is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is typically just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Set 1-24 for multiplexing barcoded samples on Ion S5 chips.

Learn more about Oncomine cfDNA Assays >


Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit (Ion Torrent™)

The Ion AmpliSeq Transcriptome Mouse Gene Expression Assay allows simultaneous gene expression measurement of over 20,000 mouse RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA, the Ion AmpliSeq Transcriptome Mouse Gene Expression Assay is ideal for those interested in obtaining comprehensive and highly sensitive gene expression measurements from a wide range of RNA sources. This particular kit enables manual library preparation with throughput flexibility. For automated production, see the Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit.

The Ion AmpliSeq Transcriptome Mouse Gene Expression Assay offers:
• Flexible, straightforward library preparation—start with as little as 10 ng of total RNA from tissues, cells, or other sources
• Fast gene expression profiling with simple workflow—go from RNA to data in <2 days with ~45 minutes of total hands-on time with the automated workflow of the Ion GeneStudio S5 and Ion Chef systems
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting as well as quality control metrics
• Compatibility with Ion Xpress Barcode adapters, allowing for multiplexed sequencing

Leveraging the speed and simplicity of Ion AmpliSeq technology, this ultrahigh-multiplex PCR enrichment approach enables rapid gene expression profiling with minimal hands-on time. This research method provides a faster, simpler, and lower-cost sequencing method that is highly correlated to whole-transcriptome RNA sequencing.

Straightforward library preparation and multiplex sequencing with manual kit
Starting with as little as 10 ng of total RNA, libraries can be prepared in approximately seven hours with only one hour of hands-on time. Total RNA is first processed with the SuperScript VILO cDNA Synthesis Kit (not included), and the resulting cDNA is amplified using Ion AmpliSeq technology to accurately maintain expression levels of all genes.

The resulting libraries are ready for downstream template preparation using the Ion OneTouch 2 or Ion Chef System and sequencing on the Ion GeneStudio S5 or Ion Proton System. Libraries can be barcoded for multiplexing, providing flexibility, reduced cost, and increased throughput, with the recommended 16 libraries per Ion 550 Chip and 8 libraries per Ion 540 or Ion PI chip. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Oncomine™ Myeloid Research Assay—Chef Ready (Ion Torrent™)

The Ion Torrent Oncomine Myeloid Research Assay consists of three pools of AmpliSeq oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent platforms. The assay is designed to provide sensitive and comprehensive sample amplification of relevant DNA mutations and fusion transcripts associated with myeloid disorders in a single NGS run. The panel is comprised of 40 key DNA genes and a broad fusion panel of 29 driver genes to cover the most relevant targets in major myeloid disorders: acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML).

This version of the assay is for automated Ion Torrent Chef library preparation. For manual library preparation, please see Cat. No. A32840.

Features include:
• Simple, robust workflow that encompasses mutations and fusions in two DNA and one RNA pool in a single NGS run
• Excellent coverage of challenging targets such as CEBPA and FLT3-ITDs
• Multiplex up to four samples on a single Ion 318 chip or twelve on an Ion 530 chip
• Compatible with as little as 10 ng/pool input DNA or RNA per library from blood or bone marrow samples
• Validated detection of somatic variants down to 5% allele frequency
• Simple and fast workflow produces targeted libraries in 3.5 hours typically
• For use on the Ion PGM and Ion S5 systems with manual or automated library preparation
• Compatible with the Oncomine Knowledgebase Reporter for variant annotation

The Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist hematologists in the understanding of myeloid cancer. The assay is a complete kit facilitating the amplification of multiple gene targets from blood or bone marrow samples with as little as 10 ng of input DNA or RNA per pool. Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on either the Ion PGM or Ion S5 sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatics workflows within Torrent Suite and Ion Reporter analysis software that utilize optimized variant-calling parameters for SNV, InDel, and FLT3 large internal tandem duplication (ITD) detection. Variants can be annotated in the Oncomine Knowledgebase Reporter to generate a sample-specific report. Samples can be processed quickly and easily, and variants detected and identified confidently.

ONC ION AS LIB KIT PLUS 24 RXN (Ion Torrent™)

This is a replicate of the existing AmpliSeq SKU 4488990 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

Ion Plus Fragment Library Adapters (Ion Torrent™)

The Ion Plus Fragment Library Adapters consist of standard fragment library adapter oligos for library construction of 10-20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. While library-creation kits like the Ion Plus Fragment Library Kit and Ion Xpress Plus Fragment Library Kit include standard library adapters, the need for additional adapters can sometimes arise. For example, these Ion Plus Fragment Library Adapters are suitable for use with the AB Library Builder™ System to construct automated fragment libraries. Additionally, the Long Mate-Pair Library protocol for the Ion PGM™ Sequencer requires users to purchase standard adapter oligos – a task made easy with the introduction of our Ion Plus Fragment Library Adapters.

The Ion Plus Fragment Library Adapters offer you:

• Amplification primers for PCR amplification of standard Ion Plus fragment libraries
• The convenience of premixed and appropriately diluted adapter oligos for use in Ion fragment library construction protocols including automated library construction on the AB Library Builder™ System

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Ion AmpliSeq™ Exome RDY Kit 1x8 (Ion Torrent™)

The Ion AmpliSeq Exome RDY Kit enables the fastest and simplest method of exome enrichment, now even easier with oligo pools for ultra-high multiplex PCR exome enrichment dried down in a 96-well plate. With half the number of target amplification pipetting steps compared to the original protocol and the elimination of small pipetting volumes, exome libraries can now be produced with unparalleled ease in under six hours with less than 50 minutes of hands-on time. Also, the Ion AmpliSeq Exome RDY Kit is compatible with the Ion Library Equalizer Kit for ease in library normalization. Leveraging the ultra-high multiplex PCR approach of Ion AmpliSeq technology with template preparation on the Ion Chef System and sequencing on an Ion Proton or Ion S5 System, the Ion AmpliSeq Exome RDY Kit allows for rapid sequencing of key exonic regions of the genome, going from DNA to variants in just two days.

The Ion AmpliSeq Exome RDY Kit 1x8 configuration includes the following components for eight exomes (two exomes per Ion PI v3 chip or Ion 540 Chip, and four exomes per Ion 550 Chip):

• Ion AmpliSeq Exome RDY panel: dried down oligo pools/primers in one 96-well plate with all eight rows filled (1x8), for ultra-high multiplex PCR enrichment of the exonic regions of the genome
• Ion AmpliSeq Library Kit Plus (8 exomes) for robust library preparation of Ion AmpliSeq Exome libraries using Ion AmpliSeq technology

The Ion AmpliSeq Exome RDY Kit enables you to:
• Simplify exome enrichment through the combination of dried-down primers and the Ion AmpliSeq workflow, with less than one hour of hands-on time and less than six hours total time
• Accelerate your disease research with rapid exome sequencing—DNA to variants in just two days
• Access affordable exome sequencing—no need to wait and batch dozens of samples or otherwise spend the high price of a lower-throughput run
• Maximize sequencing efficiency due to high performance specifications—designs covering >97% of the Consensus Coding Sequences (CCDS), >90% base on-target, and >90% coverage uniformity
• Identify annotated, filtered variants without bioinformatics expertise through fully integrated analysis with Torrent Suite and Ion Reporter software

Quickest exome enrichment with unprecedented simplicity
The Ion AmpliSeq Exome RDY Kit 1x8 configuration provides unmatched simplicity for exome enrichment. Primers have been dried down in a 96-well plate, using the 12 wells of a single row for each of the 12 primer pools, leading to the use of one row per exome. The Ion AmpliSeq Exome RDY S5 Kit 1x8 configuration contains a single 96-well plate, containing eight rows pre-filled with dried down primers, sufficient to prepare eight exome libraries. Additionally, the dried-down primers contain a light blue dye for easy identification of used or occupied rows. With a workflow requiring less than 50 minutes of hands-on time and no centrifugation steps, the kit enables exome library preparation in less than six hours. Additionally, the Ion AmpliSeq Exome RDY Kit maintains a low input requirement of 50 ng DNA. With the included Ion AmpliSeq Library Kit Plus, exome library preparation is more robust, resulting in higher uniformity and consistently higher library yield when performing library amplification. Also, the Ion AmpliSeq Exome RDY Kit is now compatible with the Ion Library Equalizer Kit for ultimate ease in library normalization.

Lowest cost per exome using a benchtop sequencer
The Ion AmpliSeq Exome RDY Kit allows disease researchers to access affordable exome sequencing on their benchtops without long turn-around times or the sample batching normally required to achieve a low cost per sample. Using the Ion AmpliSeq Exome RDY Kit with the Ion Proton or Ion S5 System enables you to go from DNA to variants in as little as two days. Additionally, with the most uniform exome enrichment covering >97% of the CCDS, you can achieve the most efficient sequencing with two exomes per Ion PI or Ion 540 Chip and four exomes per Ion 550 Chip.

Fully integrated analysis without bioinformatics expertise
Use of the kit with Torrent Suite and Ion Reporter software provides a simple and streamlined analysis workflow that automates the identification of your variants of interest without needing the expertise or infrastructure of a full bioinformatics team. Additionally, the Ion Proton or Ion S5 Sequencer along with Ion Reporter Software deliver both SNP and indel information along with copy number changes through a single analysis workflow, advancing your human disease research.

Oncomine™ Comprehensive Library Assay (Ion Torrent™)

The Oncomine™ Comprehensive Library Assay is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 143 unique genes to help inform drug discovery research, and clinical trial research programs. It provides the reagents for library construction and three pools—two DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per pool, the assay covers more than 2,500 amplicons and 170 biomarkers.

The Oncomine Comprehensive Library Assay includes oligos for library construction. For a kit that also includes reagents and consumables for next-generation sequencing, please see the Oncomine Comprehensive Assay.

• Enables analysis of variants across 143 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool, isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and >300 FFPE samples
• NGS results concordant with verified single gene tests [1]
• Additional relevant variants detected
• Content driven by the Oncomine™ Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published clinical trials

From sample to answer
The Oncomine Comprehensive Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds, to just a few key cancer driver variants. Enabled by Oncomine™ informatics, this filtering, and as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Comprehensive Assays >

1. Hovelson et al., 2015. Development and validation of a scalable next-generation sequencing system for assessing relevant somatic variants in solid tumors. Neoplasia 17:385-399.

Precision ID Library Kit (Ion Torrent™)

The Precision ID Library Kit is designed for rapid preparation of amplicon libraries using HID-Ion AmpliSeq ready-to-use panels, for sequencing on Ion Torrent sequencing system. This kit uses only certain components found in the Ion AmpliSeq™ Library Kit 2.0, that are needed for Precision ID applications, making the Precision ID Library Kit more customized and specific for forensic research use only.

Key features:

Simple protocol
• 96 well plate-based workflow allows easy sample management
• No library amplification with qPCR quantification

Ion AmpliSeq™ HiFi Master Mix
• Enables cleaner amplification for increased coverage uniformity
• Up to 3,072-plex PCR allows larger panel designs

Digestion reagent
• Cleaner digest allows for more efficient downstream sequencing

Benefits for HID applications:

• 1 ng of DNA input is recommended for the Precision ID panels
• Less components to help save time and money by preventing unnecessary waste

Scalable multiplex PCR reactions
The Precision ID Library Kit is built on Ion AmpliSeq chemistry, which enables scalable multiplex PCR reactions from 12- to 3,072-plex in a single well using just 1 ng of starting DNA. Ion AmpliSeq panels and primer pools allow highly multiplexed PCR amplification of thousands of genomic target regions, with superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets.

Barcoded library preparation
The Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress™ Barcode Adapters 1–96 kits and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost and allow for accurate sample-to-sample comparisons.

Easy and flexible protocol
The Precision ID Library Kit uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories.

The resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP or STR Genotyper plug-ins enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.

*Using the Applied Biosystems™ Precision ID NGS System for Human Identification

Genexus™ Barcodes 65-96 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 64-96 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 65-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems (Applied Biosystems™)

Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems is a reagent component of the AB Library Builder™ System. This kit, along with separately purchased Agencourt® AMPure® Beads, allows for the creation of up to 13 RNA whole transcriptome libraries on the Library Builder™.
• Automation – reduce hands on time by 50% or more depending on the number of libraries
• Scalable – prepare up to 13 libraries per run, up to 26 libraries per day
• Integrated – compatible with the SOLiD® 4 System and 5500 Series Genetic Analysis Systems

Labor Saving Automation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems when used with the AB Library Builder™ System automates the most labor-intensive parts of RNA whole transcriptome library preparation, helping to reduce hands-on time by at least 50%. The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems contains enough reagents and materials to process up to 13 samples at a time.

Integrated RNA Library Preparation
The Library Builder™ Whole Transcriptome Core Kit for 5500 Genetic Analysis Systems, along with the AB Library Builder™ System, is an integrated solution comprising a predefined software protocol, plug-and-play cartridge format reagents, and instrument. These kits are all optimized and validated for RNA purification and RNA whole transcriptome library preparation with the 5500 and SOLiD® 4.

To further maximize automation consider using RNA purification protocols with the iPrep™ PureLink® Total RNA Kit, compatible with the Library Builder™ System.

For Research Use Only. Not for use in diagnostics procedures.

Ion AmpliSeq™ Cancer Hotspot Panel v2 (Ion Torrent™)

The Ion AmpliSeq™Cancer Hotspot Panel v2 is a single pool of primers used to perform multiplex PCR for preparation of amplicon libraries from genomic "hot spot" regions that are frequently mutated in human cancer genes (download the list of targeted genes and mutations for the Ion AmpliSeq™Cancer Hotspot Panel v2). Building on the mutations included in our original Ion AmpliSeq™Cancer Panel, the latest Ion AmpliSeq™ready-to-use panel provides:
• Maintained compatibility with FFPE samples while expanding mutational content for broader coverage of additional genes and "hot spot" mutations
• Extremely uniform coverage for more efficient sequencing and cost savings
• Enhanced primer design with optimization of new primer sets and even lower strand bias for added confidence in accurate variant calling
• Improved variant detection with Torrent Suite Software v3.0 and Variant Caller Plugin in low allele variant detection along with improved indel sensitivity

Additional Mutational Content for Advancing Cancer Research Studies
The Ion AmpliSeq™Cancer Hotspot Panel v2 is designed to amplify 207 amplicons covering approximately 2,800 COSMIC mutations from 50 oncogenes and tumor suppressor genes. This includes the 739 COSMIC mutations from 46 genes in the first Ion AmpliSeq™Cancer Panel along with added hotspot mutations from significant cancer genes. While maintaining our compatibility with FFPE samples and covering the same targets as the first Ion AmpliSeq™Cancer Panel, we have included additional mutations by adding several amplicons for hotspots in EZH2, GNA11, GNAQ, and IDH2, and by adding slightly longer amplicons for other genes to help advance your cancer research. The Ion AmpliSeq™Cancer Hotspot Panel v2 should be used with the Ion AmpliSeq™Library Kit 2.0.

More Efficient and More Cost-effective Sequencing with Extremely Uniform Coverage
The improved primer design in the Ion AmpliSeq™Cancer Hotspot Panel v2 improves upon the coverage uniformity of the Ion AmpliSeq™technology. With coverage that is more uniform, a minimum depth across all amplicons can be achieved using much less sequencing throughput. Consequently, additional samples can be multiplexed using any of the Ion chips. This results in cost savings with more efficient sequencing while maintaining high confidence in variant calling. Additionally, the low strand bias in the primer sets of the Ion AmpliSeq™Cancer Hotspot Panel v2 provides further confidence in accurate variant calling.

Simplicity, Speed, and Scalability of Ion AmpliSeq™Technology
Sustaining the simplicity of the Ion AmpliSeq™technology, the Ion AmpliSeq™Cancer Hotspot Panel v2 enables cancer genetic studies from FFPE tissues with as little as 10 ng of input DNA for targeted library construction. The Ion AmpliSeq™Cancer Hotspot Panel v2 also maintains the revolutionary workflow using standard PCR equipment and a simple PCR reaction for ultra-high multiplex PCR-based target selection, with no need for extensive capital investment. Additionally, the Ion AmpliSeq™Cancer Hotspot Panel v2 continues to enable you to achieve the fastest time to targeted libraries in approximately 3.5 hours. Scalability and flexibility is also preserved with the Ion AmpliSeq™Cancer Hotspot Panel v2, allowing the ability to sequence one sample or multiplex barcoded samples using any of the Ion chips.

Improved Variant Detection with Torrent Suite Software v3.0
The intuitive Torrent Suite Software and Variant Caller Plugin enable you to go from extracted DNA to variant calls in as little as 10 hours. With improvements to algorithms, you will see more sensitive indel detection along with more sensitive SNP detection with lower frequency alleles. Ion Reporter™Software can then be used for annotation of variants in routine DNA assays for the advancement of clinical research.

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes(Set D, 73-96) (Invitrogen™)

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly