Shop All Library Preparation Kits for Sequencing

Ion AmpliSeq™ Kit for Chef DL8 (Ion Torrent™)

The Ion AmpliSeq™ Kit for Chef DL8 is designed for use with the Ion Chef™ System for automation of Ion AmpliSeq library construction. The kit offers:

• Simplest workflow for targeted sequencing library preparation using 1 or 2 pool Ion AmpliSeq panel designs*
• Low input
• Easy-to-use cartridge format with reagent barcoding for full reagent tracking
• Proven performance of Ion AmpliSeq technology, compatible with DNA extracted from formalin-fixed, paraffin-embedded tissue and other challenging sources

*See www.AmpliSeq.com for custom 1-2 pool panels. Note that some panels (e.g., Transcriptome, Cancer Hotspot, Oncomine BRCA, Pharmacogenomics) have been prepared in an Ion Chef-ready kit by incorporating the respective ready-made primer pool with the DL8 kit.

Leveraging the automation capabilities of the Ion Chef System, the Ion AmpliSeq Kit for Chef DL8 enables the reproducible preparation of Ion AmpliSeq amplicon libraries, with less than 15 minutes of hands-on time and only 3 liquid transfer steps. Compatible with 1- and 2-pool Ion AmpliSeq panel designs, the kit automates the preparation of libraries from 8 samples in a single run, delivering a single tube of pooled libraries ready for downstream templating and sequencing. The kit provides sufficient material for the preparation of 32 libraries (4 Ion Chef runs).

Utilizing plug and play, pre-packaged, single-use cartridges and PCR plates for a fully automated workflow, users of all experience levels will find library preparation straightforward with minimal effort. Supplied with barcodes conveniently dried-down in 96-well plates, multiplexing samples is easy and requires no additional pipetting steps.

More information on Ion AmpliSeq technology >

Genexus™ Barcodes 65-96 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 64-96 AS kit provides one plate filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted.When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, this kit supports flexible, fully automated preparation and normalization of up to 32 Ion AmpliSeq libraries. Choose from other available barcode kits: Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 1-96 AS. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 65-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Precision ID GlobalFiler™ NGS STR Panel v2 (Applied Biosystems™)

The Applied Biosystems Precision ID GlobalFiler NGS STR Panel v2 enables an innovative next-generation sequencing (NGS) approach to analysis of DNA mixtures for casework applications using massively parallel sequencing built on Ion AmpliSeq technology for superior efficiency. The panel includes 35 markers comprised of 21 CODIS STR markers, 9 additional multi-allelic STR markers, and 4 sex determination markers to aid in mixture interpretation of complex casework samples.

Using a minimal DNA input of 125 pg, this targeted forensic marker panel is optimized for use with the Precision ID DL8 Kit (Cat. No. A33212) and the Ion S5 Precision ID Chef & Sequencing kit (Cat. No. A33208). The automated Precision ID workflow enables streamlined sample extraction-to-genotypes using the Ion Chef System, Ion GeneStudio series sequencer, and Converge Software NGS Analysis Module.

Simplicity
• As little as 125 pg of input DNA
• Manual and automated Ion Chef library preparation
• Template preparation with the Ion Chef System
• Interpret profiles with the Converge NGS Analysis Module

Scalability
• Ion 520 or Ion 530 chip-enabled
• Ability to multiplex up to 32 samples for efficient sample processing

The Converge Software NGS module offers complete NGS data analysis of targets amplified with the Precision ID GlobalFiler NGS STR Panel v2. STR markers are displayed as both allele number and base sequence for each repeat structure, offering enhanced mixture resolution. The software also analyzes single nucleotide polymorphisms (SNPs) in flanking regions of the STR markers. This unique NGS STR panel combines maximum compatibility with CODIS core loci databasing standards and superior discrimination power, enabling forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The Precision ID GlobalFiler NGS STR Panel v2 is optimized for casework samples.

Compatible with the Ion S5/S5XL and Ion GeneStudio series systems only. Not for use with the Ion OneTouch2 System or Ion PGM System.

NOTE: The GlobalFiler NGS STR Panel v2 provides sufficient material to perform 96 reactions using the manual Precision ID Library kit (Cat. No. A26435) or 32 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212).

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems, with human/mouse/rat rRNA depletion kit and UD indexes (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow— total RNA can be converted to sequencing-ready library within 6.5 hours
• Inert dyes in the reagents—see the progress of library generation without any negative effect on quality
• Superior rRNA removal
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detection
• >98% strand specificity
• Efficient detection of non-coding RNA
• Preservation of 3‘ end sequence information

This Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation starting with sample inputs of 100 ng—1 µg of total human, mouse, or rat RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based rRNA depletion and library purification steps, enable completion of the entire workflow in approximately 6.5 hours. The kit is suitable for various-quality RNA samples, including FFPE samples.

Visual cues of process integrity
For maximum convenience, visual cues of process integrity are included throughout the library preparation workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

How it works
The Collibri Stranded RNA Library Prep Kit with Human/Mouse/Rat rRNA Depletion Kit includes ribosomal RNA (rRNA) depletion reagents to enable a comprehensive view of the transcriptome through superior removal of human, mouse, and rat rRNA. Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed or unique dual-indexed libraries compatible with single-read or paired-end sequencing. The kit contains 24 or 96 either single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer or unique dual-indexed PCR primers that enable multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit, is recommended for qPCR-based quantifications of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Identification of alternative polyadenylation sites
• Gene fusion detection
• Detection of transcript isoforms

Oncomine Focus Assay, Chef-Ready Library (Ion Torrent™)

The Oncomine Focus Assay is a targeted next-generation sequencing (NGS), multi-biomarker assay that enables you to detect variants across 52 cancer-relevant genes from DNA and RNA in a single workflow. It provides reagents for library construction and two pools—one DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per primer pool, the assay enables analysis of hotpots, SNVs, indels, CNVs, and gene fusions in a single workflow. For a kit that includes reagents for manual library preparation, please see the Oncomine Focus Assay, AmpliSeq Library.

The Oncomine Focus Assay, Chef-Ready Library, is for the Ion Chef System and Ion GeneStudio S5 System workflow. It contains all the reagents needed to prepare libraries for 32 samples:

Oncomine Focus Assay, DNA Chef-Ready panel—contains the DNA primer pool
Oncomine Focus Assay, RNA Chef-Ready panel—contains the RNA primer pool
Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System

From sample to answer
The Oncomine Focus Assay is part of an NGS workflow that helps researchers filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.Learn more about Oncomine Focus Assays and Oncomine Oncology.

IonCode Barcode Adapters, 385-768 (Ion Torrent™)

IonCode Barcode Adapters 385-768 are 384 unique barcode adapters designed for optimal performance with Ion Torrent semiconductor sequencers. This set includes IonCode Barcode Adapters 0501-0596, 0601-0696, 0701-0796, and 0801-0896. When used in combination with other IonCode barcode adapters and the AgriSeq HTS Library Kit, users can pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, which helps increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance enables identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification

Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries enables more cost effective runs by permitting up to 768 samples per run, and helps significantly decrease the cost and handling requirements of sequencing. Each barcode was individually evaluated to verify no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to verify balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figures below).

Ion 510™ Food Protection Chip Kit (Thermo Scientific™)

Simplify sample tracking and sequencing using this Chip Kit as part of a complete Thermo Scientific™ NGS Workflow for Food Authenticity.

Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems (Invitrogen™)

The Invitrogen Collibri Stranded RNA Library Prep Kit for Illumina Systems is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) systems. The kit brings together the superior features of SuperScript IV Reverse Transcriptase, Dynabeads magnetic particles, and Platinum SuperFi DNA Polymerase, enabling users to obtain high-quality sequencing-ready libraries.

The Collibri Stranded RNA Library Prep Kit offers:
• Short, automation-friendly workflow. mRNA can be converted to sequencing-ready library within 4.5 hours.
• Inert dyes in the reagents that make it possible to see the progress of library generation without any negative effect on library quality
• Uniform transcript coverage and high transcript detection sensitivity
• High sensitivity of differential gene expression detectio
• >98% strand specificity
• Efficient detection of non-coding RNA

The Collibri Stranded RNA Library Prep Kit is designed for fast and convenient library preparation targeting insert sizes of ~150 bp for mRNA or whole transcriptome libraries with sample inputs of 1–25 ng of mRNA-enriched or ribo-depleted RNA. Single-tube adapter ligation and reverse transcription reactions, as well as magnetic bead-based library purification steps, enable completion of the entire workflow in approximately 4.5 hours. The kit is suitable for various quality RNA samples, including FFPE samples.

For maximum convenience, visual cues of process integrity are included throughout the library prep workflow. Monitor progress of library generation using visual feedback from dyes in critical reagents. The reaction mix changes color in response to the addition of critical components at each step to ensure that library preparation has the highest chance of success. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

Full-length Illumina-compatible adaptors are introduced by PCR amplification using Platinum SuperFi DNA Polymerase to construct single-indexed libraries compatible with single-read or paired-end sequencing. The Collibri Stranded RNA Library Prep Kit contains 24 or 96 single-indexed barcoded PCR primers (i7) pre-mixed with a universal i5 primer that enables multiplexing of up to 96 libraries. Optimized cleanup steps efficiently remove residual primers and adapter/primer dimers while preserving high library yields.

A related product, the Collibri Library Quantification Kit is recommended for qPCR-based quantification of libraries before proceeding to sequencing.

Applications
The Collibri Stranded RNA Library Prep Kit is recommended for:
• Gene expression studies
• Alternative splicing analysis
• Non-coding RNA detection and discovery
• Gene fusion detection
• Detection of transcript isoforms

ONC ION AMPLISEQ FOR CHEF, 4x DL8 (Ion Torrent™)

This is a replicate of the existing AmpliSeq SKU A89024 for revenue under the CS4 PL to be bundled with Oncomine tumor specfic panels.

Ion Xpress™ Barcode Adapters 1-96 Kit (Ion Torrent™)

The Ion Xpress™ Barcode Adapter 1-96 Kit provides an extensive set of 96 unique barcode adapters specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, you may pool from 2 to 96 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the next generation sequencing workflow for a wide range of applications, including targeted resequencing.

Key Product Features:

• Enables multiplexing of 96 fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adapters Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adapter sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not for use in diagnostic procedures.

Oncomine™ BRCA Research Assay, Manual Library Preparation (Ion Torrent™)

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms. The assay is designed to provide sensitive and comprehensive sample amplification of all coding regions of the human BRCA1 and BRCA2 genes. This version of the assay is for manual library preparation. For automated library preparation using the Ion Chef™ System, please see Cat. No. A32841.

Features include:

• Validated for use on Ion 318 and Ion 530 chips
• Detects SNVs, InDels, and large exon/gene deletions/duplications
• Compatible with as little as 10 ng input DNA from formalin-fixed, paraffin-embedded (FFPE) tissue or blood samples
• Highly uniform coverage across all coding exons and splice sites for efficient sequencing and accurate analysis
• Optimized primer design and amplification chemistry enable highly specific target enrichment
• Validated detection of somatic variants to 5% and lower
• Simple and fast workflow produces targeted libraries in 3.5 hours typically

The Oncomine BRCA Research Assay is a complete kit facilitating the amplification of the entire exonic region of both BRCA genes from FFPE tissue or blood samples with as little as 10 ng of input DNA. Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on either the Ion PGM™ or Ion S5™ sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatic workflows within Torrent Suite™ and Ion Reporter™ analysis software that utilize optimized variant calling parameters for SNV, InDel, and large exon/gene deletion/duplication detection. Samples can be processed quickly and easily, and variants detected and identified confidently in either germline or somatic DNA analysis pipelines.

BRCA1 and BRCA2 genes are tumor suppressor genes that code for proteins that are vital components of the homologous recombination pathway of DNA damage repair. BRCA mutations resulting in the deficiency of either gene have been shown to result in inefficient activation of this function and are linked to hereditary predisposition to cancer. Errors in the coding sequence of BRCA genes have been detected in germline and somatic mutations and also occur in tumor samples.

Ion Xpress™ Barcode Adapters 17-32 Kit (Ion Torrent™)

The Ion Xpress™ Barcode Adapters 17-32 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Key Product Features:

• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Collibri™ 3´ mRNA Library Prep Kit for Illumina™ Systems (Invitrogen™)

The Invitrogen Collibri 3’ mRNA Library Prep Kit is designed for robust construction of cDNA libraries for strand-specific RNA sequencing on Illumina next-generation sequencing (NGS) platforms. It supports library preparation from polyadenylated RNA in ~4.5 hours. The protocol generates one fragment per transcript, resulting in accurate gene expression values. Sequences obtained are close to the 3’ ends of the transcripts, so the kit is suitable for varying-quality RNA samples, including formalin-fixed paraffin-embedded (FFPE) samples. The majority of inserts are greater than 75 bp in size, corresponding to final library fragment sizes ≥200 bp. Multiplexing of libraries can be carried out using up to 96 single-indexed primers.

For convenience, the Collibri 3’ mRNA Library Prep Kit provides color-coded components for visual tracking of library preparation progress. Inert dyes in the reagents provide visual confirmation of additions and mixing, but do not interfere with enzymatic reactions and do not compromise library prep and sequencing results.

Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set D, 73-96) (Invitrogen™)

The Invitrogen Collibri PCR-Free ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri PCR-Free ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Supports PCR-free protocols starting with as little as 100 ng of DNA
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PCR-Free ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step (see figure below for visual tracking). The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.The Collibri PCR-Free ES DNA Library Prep Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact DNA. The entire library preparation protocol takes about two hours for the PCR-free workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PCR-Free ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Genexus™ Barcodes 1-96 AS (Ion Torrent™)

The Ion Torrent Genexus Barcodes 1-96 AS kit provides one each of Ion Torrent Genexus Barcodes 1-32 AS, 33-64 AS, and 65-96 AS. When used in conjunction with Ion Torrent Genexus Library Strips 1 and 2-AS, it supports flexible, fully automated preparation and normalization of up to 96 Ion AmpliSeq libraries. Each plate is filled with 32 dried-down, single-use barcodes and covered with a pierceable foil seal, eliminating the risk of cross contamination between wells. The barcodes can be flexibly utilized in increments of 1 to 32, until all barcodes are exhausted. For Ion AmpliSeq HD-based assays, choose Ion Torrent Barcodes 1-32 HD.

As part of the Genexus Integrated Sequencer workflow, the Genexus Barcodes 1-96 AS kit:
• Automates library preparation for 200–400 base-read Ion AmpliSeq libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability prepare 1 to 32 single-pool libraries per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.