Shop All Library Preparation Kits for Sequencing

IonCode Barcode Adapters, 385-768 (Ion Torrent™)

IonCode Barcode Adapters 385-768 are 384 unique barcode adapters designed for optimal performance with Ion Torrent semiconductor sequencers. This set includes IonCode Barcode Adapters 0501-0596, 0601-0696, 0701-0796, and 0801-0896. When used in combination with other IonCode barcode adapters and the AgriSeq HTS Library Kit, users can pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, which helps increase lab efficiency and reduce sequencing costs per sample.

Key product features:
• Robust barcodes ligate efficiently and uniformly to generate balanced representation of libraries
• Unbiased performance enables identical genotypes independent of barcode used
• Robust error correction built in by design for added confidence in sample identification

Increase sample multiplexing without impacting data quality
Multiplexing with barcoded libraries enables more cost effective runs by permitting up to 768 samples per run, and helps significantly decrease the cost and handling requirements of sequencing. Each barcode was individually evaluated to verify no bias of sequence context representation. The barcodes were also screened for equivalent efficiency to verify balanced read depth between libraries. Regardless of the specific barcode used, AgriSeq targeted genotyping-by-sequencing (GBS) generates highly uniform libraries and concordant genotype calls (see figures below).

MuSeek Index Set 1, Illumina compatible (Thermo Scientific™)

Thermo Scientific MuSeek Index Set 1, Illumina™ compatible, is designed for generation of indexed genomic DNA libraries for sequencing on the Illumina MiSeq™ and HiSeq™ instruments. The set provides 4 indexed P5 adapters and 6 MuSeek indexed P7 adapters. This enables pooling of up to 24 dual-indexed libraries and conducting multiplexed sequencing analysis. The MuSeek Index Set 1, in combination with MuSeek Library Preparation kit, Illumina™ compatible, allows fast and simple indexed library construction from 50 ng of sample DNA, eliminating the need for physical shearing, end conversion and adapter ligation steps.

Highlights

• Enables sequencing of up to 24 dual-indexed fragment libraries in a single sequencing run.

Applications

• Indexed DNA fragment library preparation for next generation sequencing.

Includes
• MuSeek Indices M501-4
• MuSeek Indices M701-6

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set C, 49-72) (Invitrogen™)

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Collibri™ PS DNA Library Prep Kit for Illumina Systems, with UD indexes (Set A, 1-24) (Invitrogen™)

The Invitrogen Collibri PS DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems. The kit is suitable for physically sheared (PS) genomes of all sizes and variable quality, including FFPE samples.

The Collibri PS DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library prep success
• Includes all necessary components in a single kit
• Offers consistent, even coverage of variable GC regions from inputs ranging from 1 to 1,000 ng
• Offers improved coverage of challenging genomic regions compared to already available library prep technologies
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri PS DNA Library Prep Kit contains inert dyes that provide visual feedback during library preparation: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with enzymatic reactions and do not compromise sequencing results.

The Collibri PS DNA Library Prep Kit consistently offers higher confidence SNP and INDEL calls resulting from stronger coverage of challenging genomic regions from a wide range of inputs (1 ng to 1 µg) of physically sheared double-stranded DNA (see figure below). Additionally, a PCR-free version is offered for inputs starting at 500 ng DNA. The entire library preparation protocol takes about 3.5 hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri PS DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

IonCode™ Barcode Adapters 1-384 Kit (Ion Torrent™)

The IonCode Barcode Adapters 1-384 Kit provides a set of 384 unique barcode adapters specifically designed for optimal performance with the Ion GeneStudio S5, Ion PGM, and Ion Proton semiconductor sequencers. When used in combination with Ion Barcode Adapters 385-768 and Ion AmpliSeq Library Kit PLUS or Ion AmpliSeq Library Kit 2.0, this kit enables users to pool up to 768 amplicon libraries and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment.

Key product features:

• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with barcode adapters enables higher throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 768 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.

Sequence- and flow-optimized for increased performance and efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design
Built-in error correction prevents the possibility of incorrect sample assignment.

Ion Xpress™ Barcode Adapters 17-32 Kit (Ion Torrent™)

The Ion Xpress™ Barcode Adapters 17-32 Kit provides a set of 16 unique barcode adaptors specifically designed and validated for optimal performance with the Ion Torrent PGM™ and Proton™ semiconductor sequencers. When used in combination with the Ion Xpress™ Plus Fragment Library Kit or the Ion Plus Fragment Library Kit, this kit enables users to pool up to 16 fragment libraries prior to emulsion PCR and then conduct multiplexed sequencing analysis, simplifying the Ion semiconductor sequencing workflow for a wide range of applications, including targeted enrichment. Use of this kit with other Barcode Adapters kits allows pooling of up to 96 fragment libraries.

Key Product Features:

• Enables multiplexing of numerous fragment library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence- and flow-optimized for equal representation all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Multiplexing with Barcode Adaptors Enables Higher Throughput
Multiplexing with barcoded libraries leads to more cost effective runs by permitting up to 96 samples per run (16 with this kit), significantly decreasing the cost and handling requirements of emPCR, enrichment, and sequencing.

Sequence- and Flow-Optimized for Increased Performance and Efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool, require a minimum number of flows to interrogate, and require only 17 bases of adaptor sequencing required which results in more economical multiplexed sequencing runs.

Robust Error Correction Built In by Design
Built-in error correction virtually eliminates the possibility of incorrect sample assignment.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Oncomine™ BRCA Research Assay, Manual Library Preparation (Ion Torrent™)

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms. The assay is designed to provide sensitive and comprehensive sample amplification of all coding regions of the human BRCA1 and BRCA2 genes. This version of the assay is for manual library preparation. For automated library preparation using the Ion Chef™ System, please see Cat. No. A32841.

Features include:

• Validated for use on Ion 318 and Ion 530 chips
• Detects SNVs, InDels, and large exon/gene deletions/duplications
• Compatible with as little as 10 ng input DNA from formalin-fixed, paraffin-embedded (FFPE) tissue or blood samples
• Highly uniform coverage across all coding exons and splice sites for efficient sequencing and accurate analysis
• Optimized primer design and amplification chemistry enable highly specific target enrichment
• Validated detection of somatic variants to 5% and lower
• Simple and fast workflow produces targeted libraries in 3.5 hours typically

The Oncomine BRCA Research Assay is a complete kit facilitating the amplification of the entire exonic region of both BRCA genes from FFPE tissue or blood samples with as little as 10 ng of input DNA. Leveraging the power of Ion AmpliSeq technology, this highly multiplexed NGS assay enables the generation of results from multiple samples in a single run. Designed for use on either the Ion PGM™ or Ion S5™ sequencing systems, results are delivered in days rather than weeks. The assay is aligned with bioinformatic workflows within Torrent Suite™ and Ion Reporter™ analysis software that utilize optimized variant calling parameters for SNV, InDel, and large exon/gene deletion/duplication detection. Samples can be processed quickly and easily, and variants detected and identified confidently in either germline or somatic DNA analysis pipelines.

BRCA1 and BRCA2 genes are tumor suppressor genes that code for proteins that are vital components of the homologous recombination pathway of DNA damage repair. BRCA mutations resulting in the deficiency of either gene have been shown to result in inefficient activation of this function and are linked to hereditary predisposition to cancer. Errors in the coding sequence of BRCA genes have been detected in germline and somatic mutations and also occur in tumor samples.

Ion 520™ Food Protection Chip Kit

Simplify sample tracking and sequencing using this Chip Kit as part of a complete Thermo Scientific™ NGS Workflow for Food Authenticity. 

Oncomine™ Comprehensive Assay v3M (Ion Torrent™)

The Oncomine Comprehensive Assay v3 is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. It provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion S5 System with the Ion 540 Chip to enable up to 8 samples (7 samples and one no-template control) per run.

When combined with Oncomine Knowledge Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

Key features of the assay are:
• Enables analysis of variants across 161 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

From sample to answer
The Oncomine Comprehensive Assay is part of an NGS workflow that helps researchers to filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

The workflow has been adopted by large-scale national clinical trial research programs including NCI-MATCH (United States) and LC-SCRUM (Japan), and includes:
• Proven Ion AmpliSeq chemistry
• Automated library and template prep on the Ion Chef System
• Scalable sequencing on the Ion S5 Sytem
• Optimized Oncomine informatics

Multiple configurations
The Oncomine Comprehensive Assay v3 comes in the following configurations to match your laboratory needs:

Manual library preparation configuration:
Oncomine Comprehensive Library Assay v3M
This configuration contains all the reagents needed to prepare libraries for 24 or 96 samples:
    • Oncomine Comprehensive Panel v3M DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3M RNA—contains the RNA primer pools
    • Ampliseq Library Plus reagents—allow you to manually create your library
Ion Chef automated library preparation configuration:
Oncomine Comprehensive Library Assay v3C
This configuration contains all the reagents needed to prepare libraries for 32 samples:
    • Oncomine Comprehensive Panel v3C DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3C RNA—contains the RNA primer pools
    • Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System


Collibri™ ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set B, 25-48) (Invitrogen™)

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Collibri™ ES DNA Library Prep Kit for Illumina Systems, with UD indexes (Set A, 1-24) (Invitrogen™)

The Invitrogen Collibri ES DNA Library Prep Kit prepares libraries for whole genome sequencing (WGS) on high-throughput Illumina next-generation sequencing (NGS) systems.

The Collibri ES DNA Library Prep Kit:
• Includes in-process visual feedback at every step of the process to confirm proper mixing of reagents for the highest chance of library preparation success
• Includes all necessary components in a single kit
• Provides up to 96 combinatorial dual or unique dual indexes
• Is applicable for automation of library preparation

The Collibri ES DNA Library Prep Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The inert dyes do not interfere with the enzymatic reactions and do not compromise the sequencing results.

The Collibri ES DNA Library Prep Kit is designed for fast and convenient library preparation from a wide range of inputs (1–500 ng) starting from intact DNA. Additionally, a PCR-free version is offered for inputs starting at 100 ng DNA. The entire library preparation protocol takes about three hours with a PCR workflow. The kit contains adapters with unique dual indexes (UDIs) or combinatorial dual indexes (CDs) that allow pooling of up to 24 or 96 different samples before the sequencing run.

Applications
The Collibri ES DNA Library Prep Kit is recommended for:
• Whole genome sequencing
• Resequencing
• de novo assembly

Oncomine™ Comprehensive Assay v3C (Ion Torrent™)

The Oncomine Comprehensive Assay v3 is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 161 unique genes to help inform drug discovery research and clinical trial research programs. It provides the reagents for library construction and four pools—two DNA and two RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Designed to help you go from hundreds down to a few relevant cancer drivers, this assay is part of a comprehensive workflow that enables NGS data analysis from as little as 10 ng of nucleic acid per pool. The assay is optimized for sequencing on the Ion GeneStudio S5 Systems with the Ion 540 Chip to enable up to 8 samples (7 samples and one no-template control) per run.

When combined with Oncomine Reporter Software, you can create reports that align labels, guidelines, and clinical trials to your results so you can focus on key drivers of cancer in your research.

Key features of the assay are:
• Enables analysis of variants across 161 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool (40 ng total) isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and controls
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence

From sample to answer
The Oncomine Comprehensive Assay is part of an NGS workflow that helps researchers to filter variant results from hundreds to just a few key cancer driver variants. Enabled by Oncomine Informatics, this filtering, as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

The workflow has been adopted by large-scale national clinical trial research programs including NCI-MATCH (United States) and LC-SCRUM (Japan), and includes:
• Proven Ion AmpliSeq chemistry
• Automated library and template prep on the Ion Chef System
• Scalable sequencing on the Ion GeneStudio S5 Systems
• Optimized Oncomine informatics

Multiple configurations
The Oncomine Comprehensive Assay v3 comes in the following configurations to match your laboratory needs:

Manual library preparation configuration:
Oncomine Comprehensive Library Assay v3M
This configuration contains all the reagents needed to prepare libraries for 24 or 96 samples:
    • Oncomine Comprehensive Panel v3M DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3M RNA—contains the RNA primer pools
    • Ampliseq Library Plus reagents—allow you to manually create your library
Ion Chef automated library preparation configuration:
Oncomine Comprehensive Library Assay v3C
This configuration contains all the reagents needed to prepare libraries for 32 samples:
    • Oncomine Comprehensive Panel v3C DNA—contains the DNA primer pools
    • Oncomine Comprehensive Panel v3C RNA—contains the RNA primer pools
    • Ion AmpliSeq Kit for Chef DL8 reagents—allows you to automate library prep on the Ion Chef System


AgriSeq™ HTS Library Kit (Applied Biosystems™)

The Applied Biosystems AgriSeq HTS Library Kit is specifically designed for high-throughput preparation of amplicon libraries for targeted genotyping-by-sequencing (GBS) applications in agrigenomics.

Key product features include:
• Exceptional uniformity of multiplexed target amplification generates robust, reproducible results
• Simplified workflow enables high-throughput sample processing
• Convenient configuration contains library normalization reagents and volumes sufficient for automated platforms

Excellent data quality
AgriSeq library preparation has been optimized for targeted GBS of panels ranging from 100–5000 amplicons. With a highly efficient and specific multiplex technology, AgriSeq amplification can generate libraries with high uniformity (87.2–99.3%), percent on-target reads (85.7–99.7%), and overall genotype call rates (87.5–99.7%) based on a set of nine recently designed panels ranging in size from 62–3000 amplicons across seven different species. The figure below shows an average of 98.2% uniformity for a 3000-amplicon panel across 192 samples (i.e., 98% of bases are covered by at least 0.2X of the average read depth). Even coverage is significant in that it reduces the amount of sequencing required to achieve consistent genotype calls, thus reducing the sequencing cost per sample. In comparison, weaker multiplexing performance is reported with the competitor technology TruSeq, which provides only 88% average uniformity and a reported 1536 maximum multiplexing capability (see reference).

High throughput sample processing
The AgriSeq Library workflow (see figure below) can be performed in either 96- or 384-well plates for high-throughput sample processing using as little as 10 ng of DNA input per reaction. The workflow has been optimized to minimize sample handling and ancillary reagent usage through the processing of pooled samples. This allows the workflow to be completed with minimal pipetting steps, helping to reduce the potential for error, as well as simplifying automation setup on common third party platforms such as Tecan or Hamilton systems. Standard data analysis processing includes automated quality assessment, alignment, and genotype calling using the included Torrent Suite software and Variant Caller plug-in without the need for a bioinformatician, expensive data storage, or third party programs.

Conveniently packaged containing library normalization reagents & volumes for automation
The AgriSeq HTS Kit is offered in large pack sizes (960 or 9600 reactions) to accommodate high throughput agrigenomics projects while minimizing the cost and waste of unnecessary packaging. The AgriSeq HTS Kit includes not only the reagents for library preparation, but all of the components required for library normalization to help achieve consistent results between samples, and sufficient overage has been provided to accommodate automated workflows. With a low price per sample exclusively available for agricultural customers, AgriSeq targeted GBS is an economical way to generate high-throughput genotyping data to advance your breeding programs.

Oncomine™ Comprehensive Library Assay (Ion Torrent™)

The Oncomine™ Comprehensive Library Assay is a targeted, next-generation sequencing (NGS) assay that enables the detection of relevant SNVs, CNVs, gene fusions, and indels from 143 unique genes to help inform drug discovery research, and clinical trial research programs. It provides the reagents for library construction and three pools—two DNA and one RNA—of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin embedded (FFPE) tumor samples. Using as little as 10 ng of nucleic acid per pool, the assay covers more than 2,500 amplicons and 170 biomarkers.

The Oncomine Comprehensive Library Assay includes oligos for library construction. For a kit that also includes reagents and consumables for next-generation sequencing, please see the Oncomine Comprehensive Assay.

• Enables analysis of variants across 143 genes
• Detection of SNVs, CNVs, gene fusions, and indels
• Robust performance from as little as 10 ng per pool, isolated from FFPE samples including fine needle biopsies
• Characterized with molecular standards and >300 FFPE samples
• NGS results concordant with verified single gene tests [1]
• Additional relevant variants detected
• Content driven by the Oncomine™ Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published clinical trials

From sample to answer
The Oncomine Comprehensive Library Assay is part of an NGS workflow that helps researchers filter variant results from hundreds, to just a few key cancer driver variants. Enabled by Oncomine™ informatics, this filtering, and as well as the annotations from on-market labels, established guidelines (US-NCCN, US-FDA, EMSO, EMA), and global clinical trials, helps you contextualize and interpret findings to move toward the answers you need.

Learn more about Oncomine Comprehensive Assays >

1. Hovelson et al., 2015. Development and validation of a scalable next-generation sequencing system for assessing relevant somatic variants in solid tumors. Neoplasia 17:385-399.

Ion AmpliSeq™ Transcriptome Human Gene Expression Panel, Chef-Ready Kit (Ion Torrent™)

The Ion AmpliSeq™ Transcriptome Human Gene Expression Panel, Chef-Ready Kit, enables automated production of Ion Ampliseq transcriptome libraries on the Ion Chef™ System. For manual library production, see the Ion AmpliSeq Transcriptome Human Gene Expression Kit.

The Ion AmpliSeq Transcriptome Human Gene Expression Panel allows simultaneous gene expression measurement of over 20,000 human RefSeq genes in a single assay. The Chef-ready kit utilizes the same chemistry as that designed for a manual workflow, providing equivalent or better performance with exceptional reproducibility, on-target reads, and sample balance. Libraries can be produced from as little as 10 ng of total RNA from FFPE or fresh/frozen samples with only 15 minutes of hands-on time to setup the Ion Chef System.

In addition, the Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit, offers:
• Wider dynamic range and improved detection sensitivity compared to conventional microarray analysis
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting, as well as quality control metrics
• Compatibility with Ion Xpress™ barcode adapters allowing for multiplex sequencing

Wider dynamic range for enhanced performance
With a wider dynamic range and improved gene detection compared to conventional microarray analysis, the Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-ready Kit, offers a clear solution to researchers needing enhanced performance for their gene expression needs. A simple workflow allows for sample-to-gene quantitation in less than 2 days, helping to reduce workload and increase throughput.

Straightforward library preparation and multiplex sequencing
The Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit, includes the reagents and materials required for automated preparation of 32 barcoded Ion AmpliSeq transcriptome libraries. The Ion Chef System can automate up to 8 barcoded libraries per run, so the kit provides reagents and materials sufficient for 4 runs. Starting with as little as 10 ng of total RNA from FFPE or fresh/frozen samples, the total RNA is first processed using the SuperScript™ VILO™ cDNA Synthesis Kit (not included), and the resulting cDNA is amplified using Ion AmpliSeq technology to accurately maintain expression levels of all genes. Approximately 7 hours of instrument run time and only 15 minutes of hands-on time are needed to set up the Ion Chef System for library construction from cDNA.

The resulting libraries are ready for downstream template preparation using the Ion Chef System and sequencing on the Ion GeneStudio™ S5™ or Ion Proton™ Sequencing System. With the recommended 16 libraries per Ion 550 Chip and 8 libraries per Ion 540 or Ion PI chip, libraries are multiplexed for flexibility, throughput increase, and cost reduction. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Automatic data analysis with Torrent Suite plug-in
Using the complementary analysis plug-in available with Torrent Suite™ software, gene quantitation information is automatically compiled, minimizing the requirement for costly computational resources and bioinformatics expertise to process raw sequencing data into meaningful information. An easy-to-interpret, highly portable table is produced containing all genes interrogated and their respective expression levels—output that may be used with a variety of existing software applications for downstream analysis of microarray or next-generation sequencing data.

Learn more about the Ion AmpliSeq Transcriptome Human Gene Expression Kit ›