Shop All Sequencing Kits

Ion PGM™ Hi-Q™ View Chef Kit Ion Torrent™

The Ion PGM™ Hi-Q™ View Chef Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 200 bp libraries on the Ion Chef™ System and the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 200 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high-accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 200 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 200 bp sequencing reactions.

For 400-base applications, order the Ion PGM™ Hi-Q™ View Chef 400 Kit (Cat. No. A30798), which provides all the reagents needed for eight 400 bp sequencing reactions.

Sanger Sequencing Kit Applied Biosystems™

The Applied Biosystems Sanger Sequencing Kit includes ExoSAP-IT Express reagent, a BigDye Terminator v3.1 Cycle Sequencing Kit, and a BigDye XTerminator Purification Kit, providing all the reagents needed to clean up PCR products, perform cycle sequencing, and clean up sequencing products. This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

ExoSAP-IT Express reagent: fastest PCR cleanup method
In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. Eliminate spin columns, magnetic beads, sedimentation, filtration, and gel purification. The novel ExoSAP-IT technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow.

• 5-min protocol
• One-tube, one-step PCR cleanup
• Novel enzyme technology
• 100% recovery of PCR products

BigDye Terminator v3.1 Cycle Sequencing Kit
The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions. The kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

BigDye XTerminator Purification Kit
The BigDye XTerminator Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer—use a single plate
• Obtain complete dye blob removal
• Stabilize samples before analysis
• Improve your sequencing workflow
• Perform manual or automated protocols
• Get rapid, reliable, and reproducible results

Ion 550™ Kit for Agrigenomics—Chef Ion Torrent™

The Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime or Ion S5 XL sequencing systems.

The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of eight loaded chips with complete run traceability.

The Ion 550 Kit, together with the Ion 550 Single Chip Supplemental Kit, enables the flexibility to prepare one or two loaded chips per Ion Chef run, without loss of templating reagents.

The Ion 550 Chip workflow:
• Provides template preparation and chip loading reagents for up to 200 base–read libraries
• Maximizes flexibility with the ability to load one or two Ion 550 chips per Ion Chef run
• Simplifies your research with ready-to-use, disposable reagent cartridges
• Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion GeneStudio S5 Plus and S5 Prime systems, along with the Ion Chef Instrument, provide reproducible sequencing workflows. Delivering up to 130 million reads in a single run, the Ion 550 Chip empowers rapid exome and transcriptome analysis, as well as high coverage for cell free nucleic acid applications. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion 550 Chip workflow provides a cost-effective and comprehensive solution for your inherited disease or oncology research needs.

Ion PI™ Hi-Q™ Sequencing 200 Kit (2 sequencing runs per initialization) Ion Torrent™

The Ion PI™ Hi-Q™ Sequencing 200 Kit contains the most advanced reagents for sequencing using the Ion Proton™ System. The kit contains a novel sequencing enzyme that enables increased variant calling accuracy. When used together with the Ion PI™ Hi-Q™ OT2 200 Kit, the Ion PI™ Hi-Q™ Sequencing 200 Kit helps to significantly increase the sensitivity and specificity of your sequence analysis. Typical applications for the Ion PI™ Hi-Q™ workflow are exome sequencing, gene panel sequencing, and transcriptome analysis. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion Proton™ system. (For 8 initializations and 8 runs, see Cat. No. A26772.)

Note: an Ion Proton™ Wash 2 Bottle is required for use of this sequencing kit.

The Ion PI™ Hi-Q™ Sequencing 200 Kit provides:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Support for sequencing using the Ion PI™ Chip, enabling up to 80 million reads per run

Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, provides fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes in your research.

Single-day sequencing workflow
The fast sequencing and data processing capabilities of the Ion Proton™ System produce sequencing results in as little as a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.

Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q™ OT2 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Sequencing 200 Kit v3.

Ion PGM™ Hi-Q™ View Chef 400 Kit Ion Torrent™

The Ion PGM™ Hi-Q™ View Chef 400 Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef 400 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef 400 Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 400 bp sequencing reactions.

For 200-base applications, order the Ion PGM™ Hi-Q™ View Chef Kit (Cat. No. A29902), which provides all the reagents needed for eight 200 bp sequencing reactions.

CarrierSeq™ ECS Kit with Ion 530™ Chips (4 samples/chip) Ion Torrent™

The Ion Torrent CarrierSeq ECS kits provide all the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.The Ion AmpliSeq CarrierSeq ECS Panel included in the kits enables the detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay.

Benefits of the CarrierSeq ECS kits include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))
• For use with Carrier Reporter, intuitive data analysis software that quickly translates data into results with customizable analysis and reporting options
• Flexible throughput options—kit formats available for 3, 4, 15, or 16 samples per chip or 6, 8, 30, or 32 samples per run

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.

Genetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

Streamlined implementation and workflow
The CarrierSeq ECS kits are an end-to-end solution that includes reagents for target amplification, library preparation, and sequencing with software for data analysis and reporting. The reagents are optimized to work together out of the box and ready to use with the Ion Chef and Ion GeneStudio S5 systems for template preparation and sequencing, respectively, and Carrier Reporter Software for reporting functionality with customizable options.

Straight-forward and powerful data analysis and reporting
Carrier Reporter Software is easy to use and designed specifically for carrier screening analysis and reporting. It incorporates the output of special analysis algorithms that address CNV and SNV/indel calling for technically challenging genes such as CYP21A2, GBA, HBA1/2, and SMN1.

ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) Ion Torrent™

The ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) includes all reagents for processing a total of 384 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 5 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 530 Chip Kit (Cat. No. A27763)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using the Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.

GX5™ Chip and Genexus™ Coupler Ion Torrent™

The Ion Torrent GX5 Chip and Genexus Coupler includes two GX5 chips and two Genexus couplers, sufficient for two initializations of the Ion Torrent Genexus Integrated Sequencer. The GX5 Chip generates 12–15 million sequencing reads per lane, or 48–60 million reads per chip. The four-lane chip design provides maximum flexibility for sample processing, enabling sequencing of one to four lanes per run, with the in-use stability to utilize any remaining lanes within two weeks of the initial run.

As part of the Genexus Integrated Sequencer workflow, the GX5 Chip:
• Achieves 12–15 million reads per lane for 200–400 base-read libraries
• Maximizes flexibility with the ability to utilize one to four chip lanes per run
• Enables parallel processing of up to four compatible assays in a single run
• Supports intake variability of clinical research samples

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef for Agrigenomics (2 sequencing runs per initialization) Ion Torrent™

The Ion 510 & Ion 520 & Ion 530 Kit-Chef (2 sequencing runs per initialization) enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system. The Ion 510 & Ion 520 & Ion 530 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. This kit accommodates 2 sequencing runs per initialization. For 1 sequencing run per initialization (required for 400 base-read applications), see Cat. No. A45849.

The Ion 510 & Ion 520 & Ion 530 Kit-Chef workflow:
• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for the Ion 510, Ion 520, and Ion 530 chips

The Ion S5 and Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 510 & Ion 520 & Ion 530 Kit with the Ion 510 Chip Kit (up to 3 million reads per chip), the Ion 520 Chip Kit (up to 5 million reads per chip), or Ion 530 Chip Kit (up to 20 million reads), allowing for flexibility of total throughput. For even greater flexibility, combine different chip types in a single Ion Chef run when using Torrent Suite Software 5.6 and above. For de novo research applications, add the Ion S5 Calibration Standard to prepared libraries for improved base-calling accuracy.

Template Generation System II Kit Thermo Scientific™

Thermo Scientific transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction is catalyzed by a single enzyme, MuA Transposase.

TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.

Features:

• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the 'shotgun' approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II Kit
• All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.

Applications
The Template Generation System II Kit is designed for inserting an artificial Mu transposon into unknown target DNA to provide primer binding sites for:

• Rapid sequencing of templates without primer walking or custom primers
• Shotgun sequencing of large DNA clones directly without time-consuming fragmentation and subcloning into a library
• Insertion of PCR priming sites into target DNA with an unknown sequence
• Random insertional mutagenesis of cloned DNA; direct amplification, mapping or sequencing using primers at the insertion site
• Sequencing with transposons vs. traditional sequencing
• Special Applications:
• BAC sequencing
• Transposition reaction into linear target DNA

BigDye™ Terminator v1.1 Cycle Sequencing Kit Applied Biosystems™

This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit Applied Biosystems™

The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

For Research Use Only. Not for use in diagnostics procedures.

CarrierSeq ™ ECS Kit with Ion 540™ Chips (16 samples/chip) Ion Torrent™

The Ion Torrent CarrierSeq ECS kits provide all the reagents and materials necessary for a comprehensive, seamless, and flexible next-generation sequencing (NGS) workflow for expanded carrier screening (ECS). When used with an Ion GeneStudio S5 System, the kits enable a simple, end-to-end workflow for the detection of carrier-positive samples by research labs interested in maximizing the identification of carrier status using genomic DNA isolated from blood or saliva samples.The Ion AmpliSeq CarrierSeq ECS Panel included in the kits enables the detection of single nucleotide variants (SNVs), insertion/deletions (indels), and copy number variants (CNVs) associated with 418 inherited disorders in a single assay.

Benefits of the CarrierSeq ECS kits include:
• Targeting of all coding regions and intron/exon boundaries of 420 genes implicated in 418 inherited disorders
• Incorporation into a single NGS assay difficult-to-characterize genes due to homology as a result of paralogues (spinal muscular atrophy (SMN1 and SMN2)), pseudogenes (Gaucher disease (GBA and GBAP1)), 21-hydroxylase deficient congenital adrenal hyperplasia (CYP21A2 and CYP21A1P), and loci (alpha-thalassemia (HBA1 and HBA2))
• For use with Carrier Reporter, intuitive data analysis software that quickly translates data into results with customizable analysis and reporting options
• Flexible throughput options—kit formats available for 3, 4, 15, or 16 samples per chip or 6, 8, 30, or 32 samples per run

Comprehensive content and consolidated assays
The Ion AmpliSeq CarrierSeq ECS Panel provides comprehensive coverage of common and rare variants to help achieve higher per-disorder detection rate. The panel targets >14,000 amplicons that cover all coding regions of 420 target genes, including intron/exon boundaries, to genotype more than 28,000 SNVs and indels from the ClinVar archive of human variation. The panel also provides robust targeting for CNV analysis to maximize carrier status detection.

Genetic variants for a number of most prevalent yet serious disorders can be difficult to resolve by NGS assays, and so separate additional stand-alone tests are often required. The Ion AmpliSeq CarrierSeq ECS Panel consolidates such stand-alone assays into a single assay, including difficult-to-sequence genes, such as SMN1 for spinal muscular atrophy, GBA for Gaucher disease, CYP21A2 for 21-hydroxylase deficient congenital adrenal hyperplasia, and HBA1 and HBA2 for alpha thalassemia.

Streamlined implementation and workflow
The CarrierSeq ECS kits are an end-to-end solution that includes reagents for target amplification, library preparation, and sequencing with software for data analysis and reporting. The reagents are optimized to work together out of the box and ready to use with the Ion Chef and Ion GeneStudio S5 systems for template preparation and sequencing, respectively, and Carrier Reporter Software for reporting functionality with customizable options.

Straight-forward and powerful data analysis and reporting
Carrier Reporter Software is easy to use and designed specifically for carrier screening analysis and reporting. It incorporates the output of special analysis algorithms that address CNV and SNV/indel calling for technically challenging genes such as CYP21A2, GBA, HBA1/2, and SMN1.

BigDye™ Terminator v3.1 Cycle Sequencing Kit Applied Biosystems™

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

Ion PI™ Hi-Q™ Chef Kit Ion Torrent™

The Ion PI™ Hi-Q™ Chef Kit enables robust and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries on the Ion Chef™ System as part of the Ion Proton™ System sequencing workflow. The plug & play, pre-packaged, single-use reagent cartridges for template and chip preparation enables an automated workflow for processing single and dual samples. The reagent and sample barcodes interact with the Ion Chef™ System to validate user-defined run plans and offer complete run traceability.

The Ion PI™ Hi-Q™ Chef kit offers:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Increased sensitivity with improved target coverage and representation
• A simplified workflow with ready-to-use Ion PI™ chips v3
• Over 60% reduction of library input amount when compared to PI™ IC 200 workflow
• Less than 15 minutes hands-on time
• Protocols for the preparation of one or two Ion PI™ chips per run
• Planned run sharing between different Ion Chef™ systems

The Ion PI™ Hi-Q™ Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow, designed to simplify and streamline the overall workflow with minimal hands-on-time for users with any experience level. This easy-to-use kit delivers high-quality and reproducible templated products for semiconductor sequencing.

The Ion PI™ Hi-Q™ Chef Kit contains sufficient reagents and consumables for 8 template preparation and sequencing runs, and should be used in conjunction with Ion PI™ chips v3 for sample tracking purposes.

Notes:
• This kit supports 200 base-read libraries for a variety of applications.
• This kit is only compatible with the Ion Chef™ and Ion Proton™ systems.
• Components of this kit have been optimized for use on the Ion Chef™ and Ion Proton™ systems specifically and should NOT be replaced or interchanged with other product kit components.
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