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Ion PGM™ Hi-Q™ View Chef 400 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef 400 Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef 400 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef 400 Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 400 bp sequencing reactions.

For 200-base applications, order the Ion PGM™ Hi-Q™ View Chef Kit (Cat. No. A29902), which provides all the reagents needed for eight 200 bp sequencing reactions.

Ion 520™ & Ion 530™ ExT Kit-Chef (Ion Torrent™)

The Ion 520™ & Ion 530™ ExT Kit-Chef enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef™ System and the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 520 & Ion 530 ExT Kit-Chef contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 4 loaded chips with complete run traceability.

The Ion 520 & Ion 530 ExT Kit-Chef and Ion S5/Ion S5 XL workflow:
• Provides template preparation and chip loading reagents for 400 to 600 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for templating and sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for both the Ion 520™ and Ion 530™ chips

The Ion S5/Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 520 & Ion 530 ExT Kit with the Ion 520 Chip Kit (up to 4 million reads per chip) or Ion 530 Chip Kit (up to 12 million reads), allowing for flexibility of total throughput. For de novo research applications, add the Ion S5™ ExT Calibration Standard to prepared libraries for greater base-calling accuracy of 400 to 600 bp fragments.

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL Systems.
Use this kit with Torrent Suite™ Software 5.2 or higher.

BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

BigDye™ Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems™)

This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Ion ReproSeq™ PGS Kit with Ion 510™ Chips (16 samples/run) (Ion Torrent™)

The Ion ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) includes all reagents for processing a total of 64 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 4 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 510 Chip Kit (Cat. No. A34288)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using an Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.

Ion PGM™ Hi-Q™ View Chef Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 200 bp libraries on the Ion Chef™ System and the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 200 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high-accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 200 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 200 bp sequencing reactions.

For 400-base applications, order the Ion PGM™ Hi-Q™ View Chef 400 Kit (Cat. No. A30798), which provides all the reagents needed for eight 400 bp sequencing reactions.

Ion ReproSeq™ PGS Kit with Ion 520™ Chips (24 samples/run) (Ion Torrent™)

The Ion ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) includes all reagents for processing a total of 96 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 4 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 520 Chip Kit (Cat. No. A27761)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using an Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.

dRhodamine Dye Terminator Ready Reaction Cycle Sequencing Kit (Applied Biosystems™)

The dRhodamine Terminator Cycle Sequencing Kit provides optimized dRhodamine dideoxy terminator chemistries for decreased background noise in a wide array sequencing applications.
• Increase your signal-to-noise ratio.
• Simplify workflows with easy-to-clean-up chemistries.
• Get optimized chemistry for sequencing templates with homo-polymers (AA's, TT's, etc.).
• Obtain clean signal, even peak patterns, and minimal mobility shifts with improved dye terminators.
• Reduce spectrum overlap with narrow dye emission spectra.

Simplify Your Sequencing Workflow
This kit's dRhodamine-dye terminator-based cycle sequencing enables you to sequence DNA rapidly and conveniently. Dye terminators allow you to set up reactions in a single tube, reduce pipetting and liquid handling, and increase sample throughput.

Higher Accuracy, Longer Read Lengths
The dRhodamine Terminator Cycle Sequencing Kit contains the same components as the standard BigDye® Terminator Kit, except that dRhodamine (dichlororhodamine) replaces rhodamine terminators, so you get more even peak patterns and less background noise. This alternative chemistry ensures that you get greater accuracy, longer read lengths, and more reliable automated sequencing.

Easy to Integrate into Your Workflow
The kit's chemistry requires no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Ion PGM™ Wash 2 Bottle Kit (Ion Torrent™)

The Ion PGM™ Wash 2 Bottle Kit is used in conjunction with the Ion PGM™ Hi-Q™ Sequencing Kit. The bottle kit contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement. The reusability of this wash bottle allows for not only improved run to run reproducibility but reduced waste and cost savings per run.

Sanger Sequencing Kit (Applied Biosystems™)

The Applied Biosystems Sanger Sequencing Kit includes ExoSAP-IT Express reagent, a BigDye Terminator v3.1 Cycle Sequencing Kit, and a BigDye XTerminator Purification Kit, providing all the reagents needed to clean up PCR products, perform cycle sequencing, and clean up sequencing products. This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

ExoSAP-IT Express reagent: fastest PCR cleanup method
In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. Eliminate spin columns, magnetic beads, sedimentation, filtration, and gel purification. The novel ExoSAP-IT technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow.

• 5-min protocol
• One-tube, one-step PCR cleanup
• Novel enzyme technology
• 100% recovery of PCR products

BigDye Terminator v3.1 Cycle Sequencing Kit
The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions. The kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

BigDye XTerminator Purification Kit
The BigDye XTerminator Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer—use a single plate
• Obtain complete dye blob removal
• Stabilize samples before analysis
• Improve your sequencing workflow
• Perform manual or automated protocols
• Get rapid, reliable, and reproducible results

Ion ReproSeq™ PGS 314 View Kit, with chips (Ion Torrent™)

The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The Ion ReproSeq™ PGS 314 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 24 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 314™ Chip Kit v2 BC (4488144)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

Ion ReproSeq™ PGS 316 View Kit, with chips (Ion Torrent™)

The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The Ion ReproSeq™ PGS 316 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 120 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 316™ Chip Kit v2 BC (4488149)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

BigDye™ Direct Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.

The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings (Figure 1).

The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction – using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction – using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).

A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.

An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer (Figure 2) or SeqStudio cartridge for faster capillary electrophoresis times. The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.

Template Generation System II Kit (Thermo Scientific™)

Thermo Scientific transposon technology is the first technology that allows scientist to insert constructed linear DNA fragment into circular or linear DNA target randomly and very efficiently. The Thermo Scientific Template Generation System II Kit (TGS II Kit) is based on the transposition reaction of the bacteriophage Mu. This system has been simplified to work in vitro, and an artificial Mu transposon, designated as the Entranceposon, has been constructed. The reaction is catalyzed by a single enzyme, MuA Transposase.

TGS II Kit provides the tools for inserting the Entranceposon into foreign DNA at random locations. Insertion of an Entranceposon into unknown target DNA provides primer binding sites for different applications. A simple and fast in vitro transposition reaction takes place in a single reaction tube.

Features:

• Provides templates for DNA sequencing more simply and with less hands-on time than any other method
• Thousands of ready-to-sequence templates from a single transposition reaction: Enough to sequence even large DNA clones (e.g. BAC clones) without fragmentation or subcloning
• Simple mapping of the Entranceposon insertions by colony-PCR or restriction enzyme digestion enables directed sequencing. Fewer sequencing reactions are required to complete a sequence than with the "shotgun" approach
• Universal primers: The sequencing and mapping primers are included in the kit, no need for custom primers.
• Bidirectional sequencing: A single template clone can be used for sequencing the flanking DNA on both sides of the Entranceposon insertion
• Three different antibiotic resistance genes are available in each TGS II Kit
• All components needed as well as detailed instruction manual to perform the reactions are included in the kit. TGS II Kit is an updated version of the TGS I Kit.

Applications
The Template Generation System II Kit is designed for inserting an artificial Mu transposon into unknown target DNA to provide primer binding sites for:

• Rapid sequencing of templates without primer walking or custom primers
• Shotgun sequencing of large DNA clones directly without time-consuming fragmentation and subcloning into a library
• Insertion of PCR priming sites into target DNA with an unknown sequence
• Random insertional mutagenesis of cloned DNA; direct amplification, mapping or sequencing using primers at the insertion site
• Sequencing with transposons vs. traditional sequencing
• Special Applications:
• BAC sequencing
• Transposition reaction into linear target DNA

Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) (Ion Torrent™)

The Ion ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) includes all reagents for processing a total of 384 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 5 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 530 Chip Kit (Cat. No. A27763)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using the Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.