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5500 W Template Amplification Kit v2 (Applied Biosystems™)

The 5500 W Template Amplification Kit v2 contains reagents needed for amplifying library template onto the 5500 W FlowChip for sequencing on the 5500 W Series genetic analyzers. The kit is comprised of a strip tube that holds the enzymes, dNTPs, and buffers, allowing automated template amplification on the 5500 W Series genetic analyzers. The kit is used for fragment, mate-paired, and paired-end sequencing runs. This kit contains sufficient reagents to run 6 lanes.

5500 W FWD SR75 Kit (Applied Biosystems™)

5500 W FWD Sequencing Reagent (SR) kits contain the phosphatase and probes needed for ligation sequencing with 2-base encoded (2BE) probes on the 5500 W series genetic analyzers. The 5500 W FWD SR75 Kit consists of an 8-well striptube that contains sufficient phosphatase and probes to run up to 510 cycles and is best configured for sequencing read-lengths up to 75 bases with a 10-base barcode for 6 lanes.

Ion PI™ Hi-Q™ Chef Kit (Ion Torrent™)

The Ion PI™ Hi-Q™ Chef Kit enables robust and reproducible template preparation, chip loading, and sequencing of 200 base-read libraries on the Ion Chef™ System as part of the Ion Proton™ System sequencing workflow. The plug & play, pre-packaged, single-use reagent cartridges for template and chip preparation enables an automated workflow for processing single and dual samples. The reagent and sample barcodes interact with the Ion Chef™ System to validate user-defined run plans and offer complete run traceability.

The Ion PI™ Hi-Q™ Chef kit offers:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Increased sensitivity with improved target coverage and representation
• A simplified workflow with ready-to-use Ion PI™ chips v3
• Over 60% reduction of library input amount when compared to PI™ IC 200 workflow
• Less than 15 minutes hands-on time
• Protocols for the preparation of one or two Ion PI™ chips per run
• Planned run sharing between different Ion Chef™ systems

The Ion PI™ Hi-Q™ Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow, designed to simplify and streamline the overall workflow with minimal hands-on-time for users with any experience level. This easy-to-use kit delivers high-quality and reproducible templated products for semiconductor sequencing.

The Ion PI™ Hi-Q™ Chef Kit contains sufficient reagents and consumables for 8 template preparation and sequencing runs, and should be used in conjunction with Ion PI™ chips v3 for sample tracking purposes.

Notes:
• This kit supports 200 base-read libraries for a variety of applications.
• This kit is only compatible with the Ion Chef™ and Ion Proton™ systems.
• Components of this kit have been optimized for use on the Ion Chef™ and Ion Proton™ systems specifically and should NOT be replaced or interchanged with other product kit components.

5500 W FWD SR Kit (Applied Biosystems™)

The 5500 W FWD Sequencing Reagent (SR) Kit contains phosphatase and probes needed for ligation sequencing with 2-base encoded (2BE) probes on the 5500 W Series genetic analyzers. The 5500 W FWD SR Kit consists of an 8-well strip tube that contains sufficient phosphatase and probes to run up to 360 cycles, or 60 cycles per lane for 6 lanes.

5500 W FWD Ligase Kit (Applied Biosystems™)

The 5500 W FWD Ligase Kit contains ligase needed for ligation sequencing on the 5500 W Series genetic analyzers. The 5500 W FWD Ligase Kit consists of an 8-well strip tube that contains sufficient ligase to run up to 360 cycles, or 60 cycles per lane for 6 lanes.

5500 W FlowChip Prep Enzyme Kit (Applied Biosystems™)

The 5500 W FlowChip Prep Enzyme Kit contains enzyme, primer, and buffer needed to deposit sample library template into the 5500 W FlowChip and prepare for template amplification on the 5500 W Series genetic analyzers. This kit is an individually orderable component of the 5500 W FlowChip Prep Pack.

The 5500 W FlowChip Prep Enzyme Kit includes:
5500 W FlowChip Prep Enzyme
5500 W FlowChip Prep Primer
5500 W FlowChip Prep Reagent 1
5500 W FlowChip Prep Reagent 2

The kit contains sufficient reagents for 24 lanes.

Ion 520™ & Ion 530™ ExT Kit-Chef (Ion Torrent™)

The Ion 520™ & Ion 530™ ExT Kit-Chef enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef™ System and the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 520 & Ion 530 ExT Kit-Chef contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 4 loaded chips with complete run traceability.

The Ion 520 & Ion 530 ExT Kit-Chef and Ion S5/Ion S5 XL workflow:
• Provides template preparation and chip loading reagents for 400 to 600 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for templating and sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for both the Ion 520™ and Ion 530™ chips

The Ion S5/Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 520 & Ion 530 ExT Kit with the Ion 520 Chip Kit (up to 4 million reads per chip) or Ion 530 Chip Kit (up to 12 million reads), allowing for flexibility of total throughput. For de novo research applications, add the Ion S5™ ExT Calibration Standard to prepared libraries for greater base-calling accuracy of 400 to 600 bp fragments.

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL Systems.
Use this kit with Torrent Suite™ Software 5.2 or higher.

SOLiD™ FWD SR S75 Kit, Single (Applied Biosystems™)

SOLiD® Sequencing Reagents (SR) Kits contain enzymes and probes needed for ligation sequencing with 2-base encoded (2BE) probes on the 5500/5500xl Genetic Analyzer. The SOLiD® FWD SR Kits are used for fragment, mate-paired, and paired-end sequencing runs, for ligation sequencing in the forward direction.

Single-sized kits have sufficient reagents for covering one FlowChip.

The SOLiD® FWD SR S75 Kit, Single contains sufficient ligase, phosphatase, and forward 2BE probes to run 375 to 516 cycles, or up to 86 cycles per lane for 6 lanes. The kit is best configured for sequencing read lengths up to 75 bp for barcoded runs. In addition to the SOLiD® Run Cycle Buffers and SOLiD® Buffer D (Reset), this kit requires the use of the SOLiD® FWD Buffer. Store the SOLiD® FWD SR S75 Kits at –25°C to –15°C until ready for use.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

BigDye™ Direct Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.

The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours – up to a 40% time savings (Figure 1).

The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction—using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction—using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).

A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.

An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer for faster capillary electrophoresis times (Figure 2). The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use

dRhodamine Dye Terminator Ready Reaction Cycle Sequencing Kit (Applied Biosystems™)

The dRhodamine Terminator Cycle Sequencing Kit provides optimized dRhodamine dideoxy terminator chemistries for decreased background noise in a wide array sequencing applications.
• Increase your signal-to-noise ratio.
• Simplify workflows with easy-to-clean-up chemistries.
• Get optimized chemistry for sequencing templates with homo-polymers (AA's, TT's, etc.).
• Obtain clean signal, even peak patterns, and minimal mobility shifts with improved dye terminators.
• Reduce spectrum overlap with narrow dye emission spectra.

Simplify Your Sequencing Workflow
This kit's dRhodamine-dye terminator-based cycle sequencing enables you to sequence DNA rapidly and conveniently. Dye terminators allow you to set up reactions in a single tube, reduce pipetting and liquid handling, and increase sample throughput.

Higher Accuracy, Longer Read Lengths
The dRhodamine Terminator Cycle Sequencing Kit contains the same components as the standard BigDye® Terminator Kit, except that dRhodamine (dichlororhodamine) replaces rhodamine terminators, so you get more even peak patterns and less background noise. This alternative chemistry ensures that you get greater accuracy, longer read lengths, and more reliable automated sequencing.

Easy to Integrate into Your Workflow
The kit's chemistry requires no new software or instrument recalibration, so you can easily integrate it into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Ion PGM™ Hi-Q™ View Chef 400 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef 400 Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef 400 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef 400 Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 400 bp sequencing reactions.

For 200-base applications, order the Ion PGM™ Hi-Q™ View Chef Kit (Cat. No. A29902), which provides all the reagents needed for eight 200 bp sequencing reactions.

Oncomine Dx Target Test (Ion Torrent™)

The Oncomine Dx Target Test is a qualitative in vitro diagnostic test that uses targeted high-throughput, parallel-sequencing technology to detect sequence variations in 23 genes in DNA and RNA isolated from formalin-fixed, paraffin-embedded tumor (FFPE) tissue samples from patients with non-small cell lung cancer (NSCLC) using the Ion PGM Dx System.

Sequence variations in NSCLC DNA for the following 23 genes have been reported: AKT1, ALK, BRAF, CDK4, DDR2, EGFR, ERBB2, ERBB3, FGFR2, FGFR3, HRAS, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, PDGFRA, PIK3CA, RAF1, RET, and ROS1. Sequence variation in NSCLC RNA for the ROS1 gene has also been reported.

The Oncomine Dx Target Test is also indicated as a companion diagnostic to identify:
• ROS1 fusion–positive NSCLC patients for treatment with XALKORI® (crizotinib)
• BRAF V600E–positive NSCLC patients for treatment with Tafinlar+Mekinist® (dabrafenib in combination with trametinib)
• EGFR L858R– and Exon 19 deletion–positive NSCLC patients for treatment with IRESSA® (gefitinib)

The Oncomine Dx Target Test is intended for use on the Ion PGM Dx System and is intended for in vitro diagnostic use by trained personnel in a professional laboratory environment.

Key features of the test are:
• Integrated bio-informatics—provides a results report at the end of the test
• Based on AmpliSeq technology, requires 10 ng per pool (20 ng total) of nucleic acid
• Provided as a complete kit for the complete workflow

From sample to answer
The Oncomine Dx Target Test is designed for use with the Ion PGM Dx System, including the instrument, reagents, and software. This system facilitates 21CFR Part 11 compliance, allowing for role-based workflows, sample and reagent tracking, and an audit trail.

Configuration
The Oncomine Dx Target Test comes with all the reagents necessary to take an FFPE sample from extraction to result. The test includes the following components to provide you with a complete NGS workflow solution:• Ion Torrent FFPE sample extraction kit
• Oncomine Dx Target Test panel and controls
• Ion PGM Dx Library Kit
• Ion OneTouch Dx Template Kit
• Ion PGM Dx Sequencing Kit
• Ion PGM Dx 318 Chip Kit
• Oncomine Dx Target Test user guides and assay definition file

5500 W FlowChip Prep Pack (Applied Biosystems™)

The 5500 W FlowChip Prep Pack contains reagents needed to deposit sample library template into the 5500 W FlowChip and prepare for template amplification on the 5500 W Series genetic analyzers.

The 5500 W FlowChip Prep Pack includes:
5500 W FlowChip Prep Enzyme Kit (1)
5500 W FlowChip Preparation Kits (4)

The kits contain sufficient reagents for 24 lanes.

Ion PI™ Hi-Q™ Sequencing 200 Kit (2 sequencing runs per initialization) (Ion Torrent™)

The Ion PI™ Hi-Q™ Sequencing 200 Kit contains the most advanced reagents for sequencing using the Ion Proton™ System. The kit contains a novel sequencing enzyme that enables increased variant calling accuracy. When used together with the Ion PI™ Hi-Q™ OT2 200 Kit, the Ion PI™ Hi-Q™ Sequencing 200 Kit helps to significantly increase the sensitivity and specificity of your sequence analysis. Typical applications for the Ion PI™ Hi-Q™ workflow are exome sequencing, gene panel sequencing, and transcriptome analysis. Kit components include sequencing supplies, enzymes, nucleotides, spike-in sequencing controls, and sequencing reagents. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion Proton™ system. (For 8 initializations and 8 runs, see Cat. No. A26772.)

Note: an Ion Proton™ Wash 2 Bottle is required for use of this sequencing kit.

The Ion PI™ Hi-Q™ Sequencing 200 Kit provides:
• The latest advancements in data quality and accuracy using the Ion Proton™ System
• Improved specificity for SNP and insertion/deletion variant calls
• Support for sequencing using the Ion PI™ Chip, enabling up to 80 million reads per run

Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, provides fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes in your research.

Single-day sequencing workflow
The fast sequencing and data processing capabilities of the Ion Proton™ System produce sequencing results in as little as a single day. With such rapid turnaround times, the Ion Proton™ System aims to be approximately three times faster than the projected performance of other next-generation sequencing platforms.

Notes
This kit is only compatible with the Ion Proton™ System.
Use this kit with the Ion PI™ Hi-Q™ OT2 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Sequencing 200 Kit v3.

Thermo Sequenase Cycle Sequencing Kit (Applied Biosystems™)

The Thermo Sequenase Cycle Sequencing Kit allows for equally efficient incorporation of both ddNTPs and dNTPs in cycle sequencing reactions resulting in very uniform band intensities which can be read accurately. The kit offers the choice of a three-dNTP extended primer method with internal α-labeled dNTPs or a 5' end-labeled primer method.

Obtain High Quality Sequence
Like Sequenase DNA Polymerase, Thermo Sequenase generates sequences with uniform band intensities and does not have the variability in band intensities of Taq DNA polymerase.

Flexible Labeling
The kit can be used for either internal labeling with α-labeled dNTPs or with 5' end-labeled primers.

Use Less Template
As little as 5 fmol of single-stranded or 20 fmol of double-stranded template is needed.

Read Through Secondary Structure
Regions with palindromes, hairpins or base repeats are more easily resolved with Thermo Sequenase and cycle sequencing methods.

Resolve Gel Compressions with 7-deaza-dGTP Nucleotide Mixes
When DNA is synthesized with 7-deaza-dGTP instead of dGTP, weaker secondary structures are formed, thus reducing compression artifacts in sequencing gels.

Eliminate Weak Bands with Pyrophosphatase
Occasional weak bands occur with prolonged reaction or cycle times. This is caused by sequence-specific pyrophosphorolysis which is catalyzed by the polymerase. The addition of pyrophosphatase minimizes pyrophosphorolysis and can prevent incorrect base-calling due to weak band intensities.

Important Notes:

The formulation of Thermo Sequenase DNA Polymerase in this kit requires the use of a glycerol tolerant DNA sequencing gel to eliminate glycerol-induced distortion of bands at approximately 350 to 600 bases beyond the primer. Information on use is included in the kit protocol. If this region is beyond your reading range, Tris-Borate-EDTA Buffer (TBE) may be used.

References:
TABOR, S. AND RICHARDSON, C. C. (1995) Proc. Natl. Acad. Sci. USA 92, p. 6339-6343.
SAMOLS, S. B., MCARDLE, B. F., RUAN, C. C., VANDERHORN, P. B. AND FULLER, C. W. (1995) Comments 22, No. 2, United States Biochemical Corp., Cleveland, OH.
RUAN, C. C., SAMOLS, S. B. AND FULLER, C. W. (1990) Comments 17, No.1, United States Biochemical Corp., Cleveland, OH.
TABOR, S. AND RICHARDSON, C. C. (1990) J. Biol. Chem. 265, 6447-6458.