Shop All Sequencing Kits

Ion PGM™ Hi-Q™ View Chef 400 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef 400 Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 400 bp libraries on the Ion Chef™ System and is compatible with the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 400 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef 400 Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef 400 Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 400 bp sequencing reactions.

For 200-base applications, order the Ion PGM™ Hi-Q™ View Chef Kit (Cat. No. A29902), which provides all the reagents needed for eight 200 bp sequencing reactions.

Oncomine Dx Target Test (Ion Torrent™)

The Oncomine Dx Target Test is a qualitative in vitro diagnostic test that uses targeted high-throughput, parallel-sequencing technology to detect sequence variations in 23 genes in DNA and RNA isolated from formalin-fixed, paraffin-embedded tumor (FFPE) tissue samples from patients with non-small cell lung cancer (NSCLC) using the Ion PGM Dx System.

Sequence variations in NSCLC DNA for the following 23 genes have been reported: AKT1, ALK, BRAF, CDK4, DDR2, EGFR, ERBB2, ERBB3, FGFR2, FGFR3, HRAS, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, PDGFRA, PIK3CA, RAF1, RET, and ROS1. Sequence variation in NSCLC RNA for the ROS1 gene has also been reported.

The Oncomine Dx Target Test is also indicated as a companion diagnostic to identify:
• ROS1 fusion–positive NSCLC patients for treatment with XALKORI® (crizotinib)
• BRAF V600E–positive NSCLC patients for treatment with Tafinlar+Mekinist® (dabrafenib in combination with trametinib)
• EGFR L858R– and Exon 19 deletion–positive NSCLC patients for treatment with IRESSA® (gefitinib)

The Oncomine Dx Target Test is intended for use on the Ion PGM Dx System and is intended for in vitro diagnostic use by trained personnel in a professional laboratory environment.

Key features of the test are:
• Integrated bio-informatics—provides a results report at the end of the test
• Based on AmpliSeq technology, requires 10 ng per pool (20 ng total) of nucleic acid
• Provided as a complete kit for the complete workflow

From sample to answer
The Oncomine Dx Target Test is designed for use with the Ion PGM Dx System, including the instrument, reagents, and software. This system facilitates 21CFR Part 11 compliance, allowing for role-based workflows, sample and reagent tracking, and an audit trail.

Configuration
The Oncomine Dx Target Test comes with all the reagents necessary to take an FFPE sample from extraction to result. The test includes the following components to provide you with a complete NGS workflow solution:• Ion Torrent FFPE sample extraction kit
• Oncomine Dx Target Test panel and controls
• Ion PGM Dx Library Kit
• Ion OneTouch Dx Template Kit
• Ion PGM Dx Sequencing Kit
• Ion PGM Dx 318 Chip Kit
• Oncomine Dx Target Test user guides and assay definition file

dGTP BigDye™ Terminator v3.0 Ready Reaction Cycle Sequencing Kit (Applied Biosystems™)

The dGTP BigDye® Terminator Cycle Sequencing Kit's fluorescent dideoxy terminator chemistry is optimized for sequencing through GT- and G-rich templates.

• Ensure reliable results with difficult regions and finishing applications.
• Sequence templates with challenging sequence contexts.
• Sequence problematic templates that standard chemistries cannot.

Sequence Challenging Templates
The dGTP BigDye® Terminator Kit is ideal for working with GT- and G-rich and other difficult-to-sequence templates. The dGTP BigDye® Terminator Kit replaces dITP with dGTP and it enables you to extend through those difficult-to-sequence regions with confidence, avoiding early signal loss in these samples.

Optimized for GT-Rich Templates
Obtain higher success rate even with your difficult GT-rich templates. By using the same chemistries as the standard BigDye® Terminator Kits (only replacing dITP with dGTP) the dGTP BigDye® Terminator Kit provides proven performance, optimized for GT-rich and other difficult-to-sequence templates.

For Research Use Only. Not for use in diagnostics procedures.

BigDye™ Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems™)

This BigDye® Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

• Improve the quality of your results for a wide range of sequencing applications.
• Sequence challenging templates and sequences more successfully.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Tackle Tough Sequences
With the BigDye Terminator v1.1 Cycle Sequencing Kit you get a robust, highly flexible chemistry for a wide range of applications, including de novo sequencing and resequencing.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye Terminator v1.1 kit's superior chemistry, you generate data with uniform peak heights and optimized signal balance-which results in longer, higher-quality reads. You also get more accurate base assignments for heterozygote and mutation detection.

Easily Integrate into Your Workflow
BigDye® Terminator v.1.1 kit's chemistry requires no new software or instrument recalibration.You can easily integrate either kit into your current workflow with minimal changes to your protocols.

For Research Use Only. Not for use in diagnostics procedures.

Ion 520™ & Ion 530™ ExT Kit-Chef (Ion Torrent™)

The Ion 520™ & Ion 530™ ExT Kit-Chef enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef™ System and the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 520 & Ion 530 ExT Kit-Chef contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 4 loaded chips with complete run traceability.

The Ion 520 & Ion 530 ExT Kit-Chef and Ion S5/Ion S5 XL workflow:
• Provides template preparation and chip loading reagents for 400 to 600 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for templating and sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for both the Ion 520™ and Ion 530™ chips

The Ion S5/Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 520 & Ion 530 ExT Kit with the Ion 520 Chip Kit (up to 4 million reads per chip) or Ion 530 Chip Kit (up to 12 million reads), allowing for flexibility of total throughput. For de novo research applications, add the Ion S5™ ExT Calibration Standard to prepared libraries for greater base-calling accuracy of 400 to 600 bp fragments.

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL Systems.
Use this kit with Torrent Suite™ Software 5.2 or higher.

Ion ReproSeq™ PGS 316 View Kit, with chips (Ion Torrent™)

The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The Ion ReproSeq™ PGS 316 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 120 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 316™ Chip Kit v2 BC (4488149)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

BigDye™ Direct Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.

The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings (Figure 1).

The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction – using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction – using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).

A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.

An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer (Figure 2) or SeqStudio cartridge for faster capillary electrophoresis times. The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.

Ion PGM™ Hi-Q™ View Sequencing Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Sequencing Kit contains reagents and consumables for robust and highly accurate sequencing of 200 and 400 base pair libraries using the Ion OneTouch™ 2 System combined with the Ion PGM™ System. This kit offers the most advanced sequencing chemistry available to users of the Ion PGM System, and its economical price brings next-generation sequencing capability to every lab. Use the Ion PGM Hi-Q View Sequencing Kit to maximize your variant calling performance.

Note: an Ion PGM™ Wash 2 Bottle Kit is required for use of this sequencing kit. One Wash 2 Bottle kit may be used for up to 40 sequencing runs before replacement.

• Delivers the latest advancements to data quality and accuracy using the Ion PGM System
• Provides a single unified solution for both 200 and 400 bp sequencing
• Offers low cost per sample through the use of multiplexing

Maximize your results with high accuracy sequencing chemistry
The Ion PGM Hi-Q View Sequencing Kit is configured to support all of your sequencing needs for applications requiring 200 bp or 400 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and Ion 318™ Chip v2 BC and is compatible with the Ion PGM™ Hi-Q™ View OT2 Kit. The kit components include sequencing solutions, sequencing reagents, and supplies.

Ion AmpliSeq ERCC Companion Panel (Ion Torrent™)

The Ion AmpliSeq RNA ERCC Companion Panel works with the ERCC RNA Spike-In Mix to provide performance assessment of Ion AmpliSeq Transcriptome libraries in gene expression experiments. Add the ERCC RNA Spike-In Mix to each RNA sample, then amplify targets with the Ion AmpliSeq Transcriptome panel plus the Ion AmpliSeq RNA ERCC Companion Panel during library preparation. When analyzing the sequencing results, the data from sequenced ERCC targets is compared to the known concentration of the ERCC targets in the Spike-In Mix.

Working with the ERCC RNA Spike-In Mix, the Ion AmpliSeq RNA ERCC Companion Panel offers:
• Standard baseline of Ion AmpliSeq Transcriptome gene expression experiments across multiple experiments and various platforms
• Measurement of the lower limit of detection and dynamic range of an expression experiment
• Identification of degraded or low-yield samples by assessing the performance of ERCC targets

Variation in RNA expression data can be attributed to a variety of factors including the quality of the starting material, the level of cellularity and RNA yield, the platform employed, and the person performing the experiment. To control for these sources of variability, a common set of external RNA controls has been developed by the External RNA Controls Consortium (ERCC), an ad-hoc group of academic, private, and public organizations hosted by the National Institute of Standards and Technology (NIST). The controls consist of a set of unlabeled, polyadenylated transcripts designed to be added to an RNA analysis experiment after sample isolation, in order to measure against defined performance criteria.

The Ion AmpliSeq RNA ERCC Companion Panel consists of 10 primer pairs targeting 10 selected transcripts from the ERCC RNA Spike-In Mix representing a 13log2 dynamic range. Chosen for general GC balance and amenability for linear amplification (TaqMan qPCR), the selected ERCC targets produce R2 >0.9 under normal experimental conditions.

Ion ReproSeq™ PGS 318 View Kit, with chips (Ion Torrent™)

The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The Ion ReproSeq™ PGS 318 View Kit, with chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement
• Ion 314/316/318™ Chip Kit v2 BC (4488150)—contains 8 barcoded chips per kit for sample tracking and sequencing runs using the Ion PGM System

Notes
• An Ion OneTouch™ ES (enrichment system) and Ion PGM™ Enrichment Beads (4478525) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

Ion PGM™ Hi-Q™ View Chef Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View Chef Kit enables accurate and reproducible template preparation, chip loading, and sequencing of 200 bp libraries on the Ion Chef™ System and the Ion PGM™ System. The plug-and-play, pre-packaged, single-use reagent cartridges enable a fully automated workflow, processing two samples per run for increased productivity. Reagent and sample barcoding features help to verify the user-defined run plan and offer complete run traceability.

• Delivers automated template preparation and chip loading for 200 bp libraries
• Provides the most accurate sequencing chemistry available for the Ion PGM System
• Designed to minimize the hands-on time required to prepare your samples
• Helps increase lab throughput with dual sample processing

Maximize your results with high-accuracy sequencing chemistry
The Ion PGM Hi-Q View Chef Kit is an integral component of the Ion Torrent™ next-generation sequencing workflow. Designed to simplify the overall workflow with minimal hands-on time, the Ion Chef System is ideal for users of all experience levels. The Ion PGM Hi-Q View Chef Kit contains the most advanced templating and sequencing chemistries available to Ion PGM customers. Use the Ion PGM Hi-Q View Sequencing Kit to help maximize your variant calling performance and improve de novo sequencing applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View Chef Kit is configured to support all of your sequencing needs for applications requiring 200 bp read lengths. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight 200 bp sequencing reactions.

For 400-base applications, order the Ion PGM™ Hi-Q™ View Chef 400 Kit (Cat. No. A30798), which provides all the reagents needed for eight 400 bp sequencing reactions.

Ion ReproSeq™ PGS 318 View Kit, without chips (Ion Torrent™)

The Ion ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow for rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for labs interested in rapidly and reliably assessing pre-implantation embryos for research. Using as little as 6 pg of DNA from a single cell, Ion ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.* The Ion PGM sequencing system provides throughput flexibility, enabling the cost-effective screening of 2 to 24 samples and minimizing the need to batch samples.

*Turnaround time for the Ion ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility
The Ion ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose the one that fits your throughput needs from a selection of 3 sequencing chips for the processing of 2 to 24 samples, thereby minimizing the need for batching to achieve cost efficiency.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the Ion ReproSeq PGS View kits offer a fast benchtop NGS workflow for aneuploidy analysis. Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours when screening up to 2 samples or as little as 10 hours with up to 24 samples. This rapid turnaround time enables fast identification of chromosomally normal embryos for research.

The Ion ReproSeq™ PGS 318 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples. The kit contains:

• Ion SingleSeq™ Kit (24 reactions/kit)—allows for the extraction, amplification, and barcoding of genomic DNA, with completion in less than 4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit)—enables the fastest template preparation (2 hours, including hands-on time) whereby DNA is clonally amplified onto a bead surface through a non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp)—offers our most advanced sequencing chemistry and is configured to support all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591)—contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement

Notes
• An Ion OneTouch™ ES (enrichment system), Ion PGM™ Enrichment Beads (4478525), and Ion 318™ Chip Kit v2 BC (4488150) are required for use with this sequencing kit.
• This kit is only compatible with the Ion PGM System.

BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.

Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) (Ion Torrent™)

The Ion ReproSeq PGS Kit for the Ion S5 System provides all the reagents and materials necessary for a rapid, cost-effective, and comprehensive next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with an Ion S5 sequencing system and Ion Chef System for automated template preparation, the kit enables a simple, end-to-end workflow for the detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes) for research labs interested in reliably assessing pre-implantation embryo biopsy samples.

Using as little as 6 pg of DNA from a single cell, or input from multiple cells, the Ion ReproSeq PGS Kit for the Ion S5 System enables accurate detection of whole chromosome and chromosome arm copy number events (down to 17 Mbp copy number events*) in as little as 10 hours**. Three kit configurations provide comprehensive sample throughput flexibility enabling cost-effective screening of 16, 24, or 96 samples per run. When combined with analysis using Ion Reporter v5.4 or later, data interpretation is enhanced with new functionality such as mosaicism detection, gender masking, and improved data plotting.

Cost-effective NGS workflow with sample throughput flexibility
The Ion ReproSeq PGS Kit for the Ion S5 System delivers scalability and flexibility through three sequencing chips—the Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip—to support various levels of sample throughput. Choose the kit that fits your throughput needs to process 16, 24, and up to 96 samples to achieve throughput flexibility that scales up to improve cost efficiencies and allows your laboratory to respond to sample fluctuation and increases in sample volume.

Rapid turnaround for time-sensitive samples
Leveraging the speed of semiconductor sequencing and automated template preparation by isothermal amplification on the Ion Chef System, the Ion ReproSeq PGS Kit for the Ion S5 System offers a fast benchtop NGS workflow for aneuploidy analysis studies. Go from a single cell or multiple cells isolated from a pre-implantation embryo to analyzed data typically in less than 10 hours when processing up to 16 samples, 10 hours for 24 samples, or 13 hours for up to 96 samples. This rapid turnaround time enables reliable identification of chromosomally normal embryos for time-sensitive genetic research.

The Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) includes all reagents for processing a total of 384 samples through a workflow that includes whole genome amplification, library construction, template preparation and chip loading on the Ion Chef instrument, and sequencing using an Ion S5 System.

The kit contains:
• Ion SingleSeq 96 Kit (Cat. No. A34763)—includes reagents to extract, amplify, and barcode genomic DNA for preparing libraries from pre-implantation embryo biopsy samples, a process that can be completed in less than 5 hours (includes hands-on time)
• Ion S5 ExT Chef Supplies, Ion S5 Chef Solutions, and Ion S5 ExT Chef PGS Reagents—contains pre-packaged single-use cartridges for automated template preparation and chip loading (5 hours, includes hands-on time) whereby library DNA is clonally amplified onto a bead surface through an emulsion-based isothermal amplification reaction
• Ion S5 ExT Sequencing Solutions, Ion S5 ExT Sequencing Reagents—simplifies sequencing with ready-to-use, disposable reagent cartridges for fast and simple instrument set-up, with less than 15 minutes hands-on time, for a sequencing chemistry supporting advanced data quality and accuracy
• Ion 530 Chip Kit (Cat. No. A27763)—contains 4 barcoded chips per kit for sample tracking and sequencing runs using the Ion S5 System

Notes
• This kit is only compatible with the Ion Chef System and Ion S5 systems. The Ion S5 XL System is recommended for rapid turnaround time between sequencing runs. Use of the kits with the Ion S5 System requires significantly more analysis time between runs.
• The Ion ReproSeq PGS Kit is compatible with Torrent Suite Software 5.4 and later. Update your Torrent Server to the latest available version of Torrent Suite Software before using these kits. For data analysis, Ion Reporter Software 5.4 or later is recommended.

*Detecting copy number events <48 Mbp using the Ion ReproSeq™ PGS Kit with Ion 530™ Chips (96 samples/run) requires running ≤24 samples on an Ion 530 Chip
**Sample to analyzed results turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is 9.6 hours.

Sanger Sequencing Kit (Applied Biosystems™)

The Applied Biosystems Sanger Sequencing Kit includes ExoSAP-IT Express reagent, a BigDye Terminator v3.1 Cycle Sequencing Kit, and a BigDye XTerminator Purification Kit, providing all the reagents needed to clean up PCR products, perform cycle sequencing, and clean up sequencing products. This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

ExoSAP-IT Express reagent: fastest PCR cleanup method
In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. Eliminate spin columns, magnetic beads, sedimentation, filtration, and gel purification. The novel ExoSAP-IT technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow.

• 5-min protocol
• One-tube, one-step PCR cleanup
• Novel enzyme technology
• 100% recovery of PCR products

BigDye Terminator v3.1 Cycle Sequencing Kit
The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions. The kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

BigDye XTerminator Purification Kit
The BigDye XTerminator Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer—use a single plate
• Obtain complete dye blob removal
• Stabilize samples before analysis
• Improve your sequencing workflow
• Perform manual or automated protocols
• Get rapid, reliable, and reproducible results