Shop All Templated Bead Preparation Kits & Reagents

Ion 550™ Chip Kit (Ion Torrent™)

The Ion 550 Chip Kit contains four (Cat. No. A34537) or eight (Cat. No. A34538) barcoded chips for sample tracking and sequencing with the Ion GeneStudio S5 Plus, the Ion GeneStudio S5 Prime, and Ion S5 XL sequencing systems. The Ion 550 Chip generates 100–130 million sequencing reads by electronically detecting polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.

The Ion 550 Chip Kit offers:
• Support for a variety of research applications, including sequencing of up to four human exomes, cell-free nucleic acid applications, transcriptome profiling, and targeted resequencing of gene panels
• A rapid run-time of as little as 2.5 hours for 200 bp sequencing
• Sample tracking functionality from an integrated barcode
Notes:The Ion 550 Chip is only compatible with the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime, and Ion S5 XL systems.
Use this chip with the Ion 550 Kit—Chef for automated template preparation and chip loading.

Ion PI™ Hi-Q™ OT2 200 Kit (Ion Torrent™)

The Ion PI™ Hi-Q™ OT2 200 Kit contains template preparation reagents for automated and reproducible template preparation using the Ion OneTouch™ 2 System (OT2) for Ion Proton™ System semiconductor sequencing. The kit is designed to deliver the highest quality template for Ion Proton™ next-generation sequencing using the Ion Onetouch™ 2 System. Use the Ion PI™ Hi-Q™ template and sequencing kits for improved variant calling accuracy. The Ion PI™ Hi-Q™ OT2 200 Kit contains template preparation reagents for sequencing 8 samples. The kit components include proprietary Ion PI™ Ion Sphere™ particles, buffers, PCR reagent and enzyme mixes, consumables, and primers.

The Ion PI™ Hi-Q™ OT2 200 Kit delivers:
• The latest advancements to data quality and accuracy using the Ion Proton™ System
• Increased sensitivity with improved target coverage and representation
• Support of Ion PI™ Chip to be sequenced with the Ion PI™ Hi-Q™ Sequencing 200 Kit

Rapid and comprehensive genomic profiling
The Ion Proton™ sequencer along with Ion AmpliSeq™ Exome technology and Ion Reporter™ Software enables comprehensive exome sequencing. Delivering up to 80 million reads in a single run, the Ion Proton™ System, used together with the Ion AmpliSeq™ Trancscriptome Human Gene Expression Kit, enables fast, cost effective, and easy gene level expression analysis of more than 20,000 RefSeq transcripts. The high quality data produced by the Ion PI™ Hi-Q™ kits paired with the Ion AmpliSeq™ Comprehensive Cancer Panel enables accurate detection of somatic variants in key tumor suppressor and oncogenes.

Automated workflow
The Ion OneTouch™ 2 System automates multiple steps to provide template preparation and enrichment. With minimal hands-on time required, it allows for preparation of multiple samples per day and significant time savings.

Notes
This kit is only compatible with the Ion OneTouch™ 2 System.
Use this kit with the Ion PI™ Hi-Q™ Sequencing 200 Kit and Ion PI™ Chip Kit v3.
This kit replaces the Ion PI™ Template OT2 200 Kit v3.

Ion 530™ Chip Kit (Ion Torrent™)

The Ion 530 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 530 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of less than 2.5 hours for 200 bp sequencing.

The Ion 530 Chip offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 15–20 million reads
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.

Ion 550™ Kit—Chef (Ion Torrent™)

The Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime or Ion S5 XL sequencing systems.

The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of eight loaded chips with complete run traceability.

The Ion 550 Kit, together with the Ion 550 Single Chip Supplemental Kit, enables the flexibility to prepare one or two loaded chips per Ion Chef run, without loss of templating reagents.

The Ion 550 Chip workflow:
• Provides template preparation and chip loading reagents for up to 200 base–read libraries
• Maximizes flexibility with the ability to load one or two Ion 550 chips per Ion Chef run
• Simplifies your research with ready-to-use, disposable reagent cartridges
• Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion GeneStudio S5 Plus and S5 Prime systems, along with the Ion Chef Instrument, provide reproducible sequencing workflows. Delivering up to 130 million reads in a single run, the Ion 550 Chip empowers rapid exome and transcriptome analysis, as well as high coverage for cell free nucleic acid applications. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion 550 Chip workflow provides a cost-effective and comprehensive solution for your inherited disease or oncology research needs.

Ion 540™ Chip Kit  (Ion Torrent™)

The Ion 540™ Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 540 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times of as little as 2.5 hours for 200 bp sequencing.

The Ion 540 Chip Kit offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of as little as 2.5 hours for 200 bp sequencing, generating 60–80 million reads
• Support for a variety of research applications, including sequencing of up to three human exomes, transcriptome profiling, and targeted resequencing of gene panels
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 540 Kit-Chef and the Ion 540 Kit-OT2.

Ion 550™ Single Chip Supplemental Kit (Ion Torrent™)

The Ion 550 Single Chip Supplemental Kit contains pre-packaged templating supplies and sequencing reagent cartridges required for use with the single-chip Ion Chef workflow. The Ion 550 Single Chip Supplemental Kit, together with the Ion 550 Kit—Chef, enables the flexibility to prepare one loaded chip per Ion Chef run, without loss of templating reagents.

Note: This supplemental templating kit for Ion Chef is only compatible with the Ion 550 Chip and the Ion 550 Kit—Chef.

Ion 520™ Chip Kit (Ion Torrent™)

The Ion 520 Chip Kit contains 8 barcoded chips for sample tracking and sequencing with the Ion S5 and Ion S5 XL Sequencing Systems. The Ion 520 Chip electronically detects polymerase-driven base incorporation without the use of fluorescence. By eliminating the use of an optical detection system, this advance in next-generation sequencing technology allows for rapid sequencing times in as little as 2.5 hours for 200 bp sequencing.

The Ion 520 Chip Kit offers:

• Sample tracking functionality from an integrated barcode
• A rapid run time of only 2.5–4 hours, generating 3–6 million reads, depending on application
• Read lengths up to 600 bp to support a variety of research applications, including targeted gene sequencing and microbial sequencing
• Easy handling and loading of templated products for sequencing
• Compatibility with current library preparation methods

Notes:
This kit is only compatible with the Ion S5 and Ion S5 XL systems.
Use this kit with the Ion 510 & Ion 520 & Ion 530 Kit-Chef, the Ion 520 & Ion 530 ExT Kit-Chef, and the Ion 520 & Ion 530 Kit-OT2.

Ion 520™ & Ion 530™ Kit-OT2 (Ion Torrent™)

The Ion 520™ & Ion 530™ Kit-OT2 enables accurate and reproducible template preparation and sequencing of up to 400 base-read libraries using the Ion OneTouch™ 2 and the Ion S5™ or Ion S5™ XL Sequencing Systems. The Ion 520 & Ion 530 Kit provides all components needed for template preparation, as well as cartridge-based sequencing reagents, delivering a semi-automated workflow for sequencing of 8 loaded chips with sequencing run traceability.

The Ion 520 & Ion 530 Kit for the Ion OneTouch 2 and Ion S5/Ion S5 XL workflow:

• Provides template preparation and chip loading reagents for up to 400 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and easy sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for both the Ion 520™ Chip and Ion 530™ Chip

The Ion S5/S5 XL sequencers, along with the Ion OneTouch 2 System, enable reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 520 & Ion 530 Kit with the Ion 520 Chip Kit (up to 5M reads per chip) or Ion 530 Chip kit (up to 20M reads per chip), allowing for flexibility of total throughput. For de novo research applications, add the Ion S5™ Calibration Standard to prepared libraries for greater base-calling accuracy.

Ion PGM™ Template IA 500 Kit (Ion Torrent™)

The Ion PGM™ Template IA 500 Kit enables accurate and reproducible template preparation of up to 500 bp libraries with the Ion OneTouch™ Enrichment System (ES) and is compatible with the Ion PGM™ system. The kit is designed to produce high-quality Ion Sphere™ particles through a single-tube chemistry that delivers isothermal amplification of long library templates onto a solid surface in as little as 40 minutes. The kit is designed for use in combination with the Ion PGM™ Hi-Q™ View Sequencing Kit.

• Delivers template preparation for up to 500 bp libraries
• Designed to provide the most rapid templating chemistry available for the Ion PGM System
• Enables increased lab throughput with multiplexing support

Increase your productivity with the fastest NGS sequencing workflow
The Ion PGM Template IA 500 Kit contains rapid and highly advanced templating chemistry, enabling template preparation in ~2 hours. Use the kit to enable the industry's fastest benchtop template preparation and sequencing workflow for applications including bacterial or viral identification, HLA typing, and aneuploidy detection of preimplantation embryos.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Template IA 500 Kit is configured to support your sequencing needs for applications requiring up to 500 bp read lengths. When combined with the Ion PGM Hi-Q View Sequencing Kit, the Ion PGM Template IA 500 Kit offers an ultra-rapid sequencing workflow. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, and the Ion 318™ Chip v2 BC. The kit components include Ion Sphere particles, solutions, reagents, and supplies for 4 templating reactions.

Ion 540™ Kit-Chef (2 sequencing runs per initialization) (Ion Torrent™)

The Ion 540™ Kit-Chef enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef™ and the Ion S5™ or Ion S5™ XL Sequencing Systems. The Ion 540 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. The kit contains sufficient reagents and supplies for 4 initializations and 8 sequencing runs on the Ion S5 or Ion S5 XL systems. For 8 initializations and 8 runs, see Cat. No. A27759.

The Ion 540 Kit for the Ion Chef and Ion S5/Ion S5 XL workflow:
• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion S5/Ion S5 XL sequencers, along with the Ion OneTouch™ 2 System, provide reproducible sequencing workflows. Delivering up to 80 million reads in a single run, the Ion 540 Chip empowers rapid exome and transcriptome analysis. Combined with Ion AmpliSeq™ technology and Ion Reporter™ Software for data interpretation, the Ion S5/Ion S5 XL systems provide a cost-effective and comprehensive solution for your translational or inherited disease research needs.

Ion PGM™ Hi-Q™ View OT2 Kit (Ion Torrent™)

The Ion PGM™ Hi-Q™ View OT2 Kit enables accurate and reproducible template preparation for libraries up to 400 base pairs using the Ion OneTouch™ 2 System and is compatible with the Ion PGM™ System. As an integral component of the semiconductor sequencing workflow, the Ion PGM Hi-Q View OT2 Kit is designed to produce high-quality Ion Sphere™ particles and is designed for use in combination with the Ion PGM™ Hi-Q™ View Sequencing Kit.

• Delivers template preparation for 200 and 400 bp libraries
• Minimizes user variability with new visible Ion Sphere particles
• Offers templating controls to help ensure high quality sequencing results
• Enables increased lab throughput with multiplexing support

Maximize your results with high accuracy templating chemistry
The Ion PGM Hi-Q View OT2 Kit is an integral component of the Ion PGM™ System sequencing workflow. Designed for users of the Ion OneTouch 2 system, the Ion PGM Hi-Q View OT2 Kit is optimized for users looking to maximize their sequencing data quality. The Ion PGM Hi-Q View OT2 Kit contains the most advanced templating chemistry available to Ion PGM customers. Use the Ion PGM Hi-Q View OT2 Kit to maximize your variant calling performance and improve de novo sequencing research applications.

A single kit to meet your Ion PGM sequencing needs
The Ion PGM Hi-Q View OT2 Kit is configured to support your sequencing needs for applications requiring 200 or 400 bp read lengths. When combined with the Ion PGM Hi-Q View Sequencing Kit, the Ion PGM Hi-Q View OT2 Kit offers optimal performance. This kit supports the Ion 314™ Chip v2 BC, Ion 316™ Chip v2 BC, Ion 318™ Chip v2 BC. The kit includes sufficient reagents for eight templating reactions. The kit components include Ion Sphere particles, buffers, reagents, and supplies.

Ion S5™ Calibration Standard (Ion Torrent™)

The Ion S5™ Calibration Standard is a small panel consisting of known sequence content with comprehensive and uniform representation of long homopolymers up to 10-mers. The Ion S5 Calibration Standard can be used in conjunction with the Ion 520™ and Ion 530™ workflows for de novo research sequencing applications to allow for greater accuracy of base-calling.

Note:
This kit is only compatible with the Ion S5™ and Ion S5™ XL Sequencing Systems.

Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (1 sequencing run per initialization) (Ion Torrent™)

The Ion 510 & Ion 520 & Ion 530 Kit-Chef (1 sequencing run per initialization) enables robust and automated template preparation and sequencing of up to 400 base-read libraries using the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system. The Ion 510 & Ion 520 & Ion 530 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. This kit accommodates 1 sequencing run per initialization. For 2 sequencing runs per initialization (200 base-read applications only), see Cat. No. A34461.

The Ion 510 & Ion 520 & Ion 530 Kit-Chef workflow:
• Provides template preparation and chip loading reagents for up to 400 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for the Ion 510, Ion 520, and Ion 530 chips

The Ion S5 and Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 510 & Ion 520 & Ion 530 Kit with the Ion 510 Chip Kit (up to 3 million reads per chip), the Ion 520 Chip Kit (up to 5 million reads per chip), or Ion 530 Chip Kit (up to 20 million reads), allowing for flexibility of total throughput. For even greater flexibility, combine different chip types in a single Ion Chef run when using Torrent Suite Software 5.6 and above. For de novo research applications, add the Ion S5 Calibration Standard to prepared libraries for improved base-calling accuracy.

Ion 510™ & Ion 520™ & Ion 530™ Kit – Chef (2 sequencing runs per initialization) (Ion Torrent™)

The Ion 510 & Ion 520 & Ion 530 Kit-Chef (2 sequencing runs per initialization) enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system. The Ion 510 & Ion 520 & Ion 530 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability. This kit accommodates 2 sequencing runs per initialization. For 1 sequencing run per initialization (required for 400 base-read applications), see Cat. No. A34019.

The Ion 510 & Ion 520 & Ion 530 Kit-Chef workflow:
• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy
• Maximizes flexibility with compatibility for the Ion 510, Ion 520, and Ion 530 chips

The Ion S5 and Ion S5 XL sequencers, along with the Ion Chef System, offer reproducible sequencing workflows, with minimal hands-on time. Choose to pair the Ion 510 & Ion 520 & Ion 530 Kit with the Ion 510 Chip Kit (up to 3 million reads per chip), the Ion 520 Chip Kit (up to 5 million reads per chip), or Ion 530 Chip Kit (up to 20 million reads), allowing for flexibility of total throughput. For even greater flexibility, combine different chip types in a single Ion Chef run when using Torrent Suite Software 5.6 and above. For de novo research applications, add the Ion S5 Calibration Standard to prepared libraries for improved base-calling accuracy.

Ion 540™ Kit-OT2 (Ion Torrent™)

The Ion 540™ Kit-OT2 enables accurate and reproducible template preparation and sequencing of up to 200 base-read libraries using the Ion OneTouch™ 2 and the Ion S5™ or S5™ XL Sequencing Systems. The Ion 540 Kit provides all components needed for template preparation, as well as cartridge-based sequencing reagents, delivering a semi-automated workflow for sequencing of 8 loaded chips with sequencing run traceability.

The Ion 540 Kit for the Ion OneTouch™ 2 and Ion S5/Ion S5 XL workflow:

• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and easy sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion S5/Ion S5 XL sequencers, along with the Ion OneTouch™ 2 system, offer reproducible sequencing workflows. Delivering up to 80 million reads in a single run, the Ion 540™ Chip empowers rapid exome and transcriptome analysis. Combined with Ion AmpliSeq™ technology and Ion Reporter™ Software for data interpretation, the Ion S5/S5 XL systems enable a cost effective and comprehensive solution for your translational or inherited disease research needs.