Shop All Luciferase Assays

Pierce™ Gaussia Luciferase Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.

Features of the Gaussia Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of Gaussia luciferase activity
Stable—increased signal stability
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other Gaussia luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.

The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc Vectors is derived from the wild type Gaussia gene and provides equally bright but more stable light output. The Gaussia Glow Assay Kit reagent yields a Gaussia-Dura luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.

Pierce™ Renilla Luciferase Flash Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

Features of the Renilla Luciferase Flash Assay Kit:

Sensitive—greater sensitivity allows utilization of smaller numbers of cells
Cost effective—highest sensitivity assays result in decreased reagent consumption
Time-saving—assays require minimal sample handling
Automation-friendly—amenable to high throughput screens
Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Pierce Renilla Flash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. The kit also is compatible for flash assays with other Renilla luciferases and derivatives that use coelenterazine as a substrate.

More Product Data
Luciferase assays in hard-to-transfect Jurkat cells
Versatile luciferases: microplate luminometers and flash luciferase assays

Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Pierce™ Cypridina Luciferase Flash Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Features of the Cypridina Luciferase Flash Assay Kit:

Sensitive—high sensitivity allows utilization of smaller numbers of cells
Cost effective—sensitive assays result in decreased reagent consumption
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Ideal for co-transfection—uses a different substrate than Renilla, Gaussia, or firefly luciferases
Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
Time-saving—assays using secreted luciferases require minimal sample handling
Automation-friendly—amenable to high throughput screens
Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. The Cypridina gene in Thermo Scientific Cypridina Luc Vectors is modified for expression in mammalian cells.

The assay kit is optimized for use with our Cypridina Luc Vectors but is fully compatible with any luciferases that use Vargulin as a substrate. Because Cypridina uses a different substrate than Renilla, Gaussia, or firefly luciferases, co-transfection and assays with up to three reporters are possible. The signal produced by Cypridina shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals
Versatile luciferases: microplate luminometers and flash luciferase assays

Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System (Invitrogen™)

The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the Luc-Screen® assay from 50 fg to 100 ng of pure enzyme in culture medium.

• Homogeneous assay format allows detection of firefly luciferase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Wide dynamic range of firefly luciferase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
Luciferase is an ideal reporter because of the high sensitivity of detection and the absence of endogenous luciferase activity in mammalian cells. The Luc-Screen® assay conveniently couples in-well cell lysis in the presence of culture medium with a high sensitivity assay that exhibits extended-glow light emission kinetics. Light signal can be measured between 10 minutes and several hours after adding assay reagents, providing great flexibility in the time between reagent addition and measurement.

High Throughput
The Luc-Screen® system is designed for maximum assay flexibility in a high throughput format and can be used in luminometers without automatic injectors. The Luc-Screen® system is formulated to provide a convenient, easy-to-use luciferase assay that is optimized for use in high-throughput screening. The high sensitivity of the Luc-Screen® assay is accompanied by a wide dynamic range.

For Research Use Only. Not for use in diagnostics procedures.

Pierce™ Renilla-Firefly Luciferase Dual Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the Renilla-Firefly Luciferase Dual Assay Kit:

Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive—measure Green Renilla and red firefly luciferase activities in the same sample
Fast—no quenching step required, unlike in traditional sequential dual assays
Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity. Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation video below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Includes:
Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Requires:
Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Applications:
• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Green Renilla produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax= 535nm) produced by Green Renilla luciferase protein results from the oxidation of coelenterazine. The second assay uses red firefly luciferase, which is a mutant form of the Japanese firefly luciferase Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP.

More Product Data
Simultaneous dual-emission detection of luciferase reporter assays

Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Pierce™ Firefly Luciferase Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.

Features of the Firefly Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Pierce Firefly Luciferase Signal Enhancer
Stable—increased signal stability compared to flash assays
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other firefly beetle luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate; purchase Luciferase Signal Enhancer separately.

Requires:
• Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
• For best results (maximum signal), use with Pierce Firefly Luciferase Signal Enhancer

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output generated by the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. The native firefly luciferase produces a strong flash signal that decays rapidly. The signal produced using the Pierce Firefly Glow Assay Kit is more stable and decays very slowly, thereby eliminating the need for a luminometer with injectors.

Pierce™ Firefly Luc One-Step Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

Features of the Firefly One-Step Luc Glow Assay Kit:

Sensitive—highly sensitive detection of firefly luciferase activity
Odorless—reagents have no foul or toxic odor, eliminating the need to work in a fume hood
Convenient—one-step, mix-and-read protocol; no additional reagents required
Stable—increased signal half-life and stability compared to flash assays
Simple—does not require a luminometer with injectors
Automation-friendly—amenable to high throughput assays

This glow-type luciferase assay kit is designed for use with cultured mammalian cells that are transfected with vectors expressing the appropriate firefly luciferase reporter enzyme. Several features make the kit especially suitable for luminometers without injectors and for high-throughput screening (HTS) applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate wells containing the transfected, treated cells. The stable signal output (glow) of this system provides flexibility with regard to incubation time. When used with Thermo Scientific Firefly Luc Plasmids, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity.

Includes:
Assay buffer and substrate solution (100X)

Requires:
Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- and trans-acting regulatory elements
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis

Firefly luciferase is a 62kDa protein produced in nature by several species of the Lampyridae family of beetles, which includes the genera Photinus and Luciola. Thermo Scientific Red Firefly Luciferase has an emission range of 560-700nm (λmax = 613nm). Bioluminescent signal from firefly luciferase originates from the oxidation of D-Luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly luciferase expression. Firefly luciferase bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Firefly luciferase reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.

Pierce™ Renilla Luciferase Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Renilla Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of Green Renilla luciferase reporter.

Features of the Renilla Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of Green Renilla luciferase activity
Stable—increased signal stability compared to flash assays
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other Renilla luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of Renilla luciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Pierce Renilla Glow Assay Kit reagent effectively stabilizes this bright bioluminescence to yield a signal half-life greater than 3 hours, thus eliminating the need for a luminometer with injectors. The assay kit was optimized for best results with Thermo Scientific Green Renilla Luc Plasmids; however, this glow kit may be used to detect the activity of other Renilla luciferase derivatives that use coelenterazine as a substrate.

Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the Cypridina-Firefly Luciferase Dual Assay Kit:

Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive—measure bright blue Cypridina and red firefly luciferase activities in the same sample
Fast—no quenching step required, unlike in traditional sequential dual assays
Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animated videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Includes:
Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin

Requires:
Cypridina and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Applications:
• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Application Videos:
Cypridina luciferase produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax=463nm) produced by Cypridina luciferase results from the oxidation of Vargulin. This reaction does not require adenosine triphosphate (ATP) or other cofactors. Red firefly luciferase is a mutant form of the Japanese firefly luciferase from Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP. Red firefly and Cypridina luciferases are used in the dual assay because the signals are spectrally resolvable.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Simultaneous dual-emission detection of luciferase reporter assays
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals

Pierce™ Cypridina Luciferase Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

Features of the Cypridina Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of Cypridina luciferase activity
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Stable—reagents provide increased signal stability compared to flash assays
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. The Cypridina gene in Thermo Scientific Cypridina Luc Vectors is modified for expression in mammalian cells and provides optimal performance with the assay kit. The Cypridina glow reagent yields a Cypridina luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.

Pierce™ Firefly Luciferase Flash Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Features of the Firefly Luciferase Flash Assay Kit:

• Highly sensitive detection of firefly luciferase activity
• Use with Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
• Working solution stable for up to 2 months at -20°C
• Assay reagents compatible with other firefly luciferases
• Amenable to automation
• Contains a universal cell lysis buffer and optimized flash assay reagent
• Safe non-radioactive assay

Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate; purchase Luciferase Signal Enhancer separately.

Requires:
• Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
• For best results (maximum signal), use with Pierce Firefly Luciferase Signal Enhancer

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.

More Product Data
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals
Versatile luciferases: microplate luminometers and flash luciferase assays

TurboLuc™ Luciferase One-Step Glow Assay Kit (Thermo Scientific™)

The Thermo Scientific™ TurboLuc™ Luciferase One-Step Glow Assay Kit measures TurboLuc™16 (Tluc16) luciferase activity in mammalian cells with a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.

• Sensitive—extremely bright, Tluc16 luciferase allows detection at a single copy per cell
• Increased dynamic range—optimized reagents result in higher signal-to-noise ratio
• Stable signal—less than 40% signal loss over 60 min duration
• Convenient—one-step, mix-and-read, scalable protocol
• Automation-friendly—amenable to HTS protocols
• Simple—does not require a luminometer with injectors

This glow-type luciferase assay kit is designed for use with cultured mammalian cells expressing the Tluc16 luciferase reporter enzyme. Tluc16 is a 16 kDa, novel intracellular luciferase derived from the marine copedod Metridia luciferase family. The wild type luciferase has been modified to reduce its size, increase its brightness, enable its intracellular expression, and optimize it for HTS. A series of vectors with and without promoters are available for expression of Tluc16 luciferase reporter in mammalian cells.

Several features of the TurboLuc Luciferase One-Step Glow Assay Kit make it suitable for luminometers without injectors and for HTS applications. The convenient, one-step, homogenous protocol minimizes handling steps to support the use of automation. Simply combine the supplied substrate solution and assay buffer to make a single working solution, and then add it to microplate containing the transfected, treated cells. The stable signal output (glow enzyme kinetics) of this system provides flexibility with regard to lead time before read. When used with Thermo Scientific TurboLuc™16 luciferase vectors, the kit provides a highly sensitive bioluminescent reporter assay system for the detection of promoter or pathway activity.

Requires:
Tluc16 luciferase assay system and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan™ Ascent and Varioskan™ Flash microplate readers.

Applications:
• Gene reporter assays
• Promoter studies for analyzing cis- and trans-acting regulatory elements
• Drug screening
• Multiplexed assays to study off-target effects
• Cell signaling or signal transduction pathway analysis

Pierce™ Gaussia Luciferase Flash Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Features of the Gaussia Luciferase Flash Assay Kit:

Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
Cost effective—highest sensitivity assays result in decreased reagent consumption
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Time-saving—assays using secreted luciferases require minimal sample handling
Automation-friendly—amenable to high throughput screens
Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our Gaussia-Dura Luc Vectors.)

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals
Versatile luciferases: microplate luminometers and flash luciferase assays

Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit (Thermo Scientific™)

The Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the Gaussia-Firefly Luciferase Dual Assay Kit:

Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive—measure bright blue Gaussia and red firefly luciferase activities in the same sample
Fast—no quenching step required, unlike in traditional sequential dual assays
Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Gaussia and Red Firefly luciferase activity. Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation videos below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Includes:
Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Requires:
Gaussia and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Gaussia Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Applications:
• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Application Videos:
Gaussia luciferase produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax= 470nm) produced by Gaussia luciferase results from the oxidation of coelenterazine. This reaction does not require adenosine triphosphate (ATP) or other cofactors. Red firefly luciferase is a mutant form of the Japanese firefly luciferase from Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP. Red firefly and Gaussia luciferases are used in the dual assay because the light output is spectrally resolvable.

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