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RiboMinus™ Eukaryote Kit v2 (Invitrogen™)

The RiboMinus™ Eukaryote Kit v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the kit include:

• Seamless integration with RiboMinus™Concentration Module (available separately) to purify RNA after ribosomal RNA depletion
• 1.5-hour workflow
• Input range of 1-5 µg

The RiboMinus™ Eukaryote Kit v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote Kit v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote Kit v2 is designed for the removal of human, mouse, and rat rRNA. The RiboMinus™ Concentration Module (available separately) is used for RNA purification after rRNA depletion. For magnetic bead-based purification, please see the RiboMinus™ Eukaryote System v2. The RiboMinus Eukaryote Kit v2 has an RNA input range of 1-5 µg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote Kit v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

Low Input RiboMinus™ Eukaryote System v2 (Invitrogen™)

The Low Input RiboMinus™ Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the 5S, 5.8S, 18S, and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the system include:

• Magnetic bead-based purification for rapid, seamless purification
• 1-hour workflow
• Low input range of 100 ng–1 µg

The Low Input RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the Low Input RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The Low Input RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA. For column-based purification, please see the RiboMinus™ Eukaryote Kit v2. The Low Input RiboMinus Eukaryote System v2 has an RNA input range of 100 ng–1 µg. For high inputs (up to 5 ug), the RiboMinus™ Eukaryote System v2 is recommended.

The Low Input RiboMinus™ Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

SequalPrep™ Normalization Plate Kit, 96-well (Applied Biosystems™)

Clean up and normalize the recovery of PCR reaction products in one step with SequalPrep™ Normalization Plates. For use with 5 µl of PCR reaction, extract up to 25 ng of products per well within a 2-3 fold concentration range.
- For use in Next Generation Sequencing platforms for normalizing pools of PCR amplicons
- Skip tedius steps required for determination of yields and re-aliquotting
- When combined with SequalPrep™ Long PCR Kit, you will get the most sensitive and reliable method for long PCR product amplification and amplicon normalization.

MagJET NGS Cleanup and Size Selection Kit (Thermo Scientific™)

Thermo Scientific MagJET NGS Cleanup and Size Selection kit, sufficient for 1 x 96 preps, is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes. The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range. The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications. No prior DNA fragment library purification is required before the cleanup and size-selection procedures.

The kit is suitable for cleanup or size selection of 5 ng to 5 µg of DNA fragment library. MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo.

Highlights

Robust—reliable cleanup and size selection from different reaction mixtures and buffers
Flexible—user-defined size-selection of exact DNA fragment range
NGS-grade purity—complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow
High throughput compatible—easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling

Applications

DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows.
Includes
• MagJET Magnetic Beads
• Binding Solution
• Wash Buffer (concentrated)
• Elution Buffer
• DNA Fragment Mix

Related Products
MagJET NGS Cleanup and Size Selection Kit

RiboMinus™ Plant Kit for RNA-Seq (Invitrogen™)

RiboMinus™ Plant Kit for RNA-Seq is the complete solution for transcriptome isolation and enrichment of the true whole transcriptome through selective depletion of ribosomal RNA in plant species. This kit offers additional plastid depletion probes designed to target chloroplast rRNA not offered in the RiboMinus™ Eukaryote Kit for RNA-Seq critical for enhanced depletion of ribosomal RNA in plants. The RiboMinus™ Plant Kit for RNA-Seq contains all the components necessary to deplete ribosomal RNA from plant species. This improvement allows for up to 99.8% depletion of the major rRNA peaks in many plant species. The RiboMinus™ Plant Kit for RNA-Seq is recommended for wheat, maize, rice, barley, soybean, sugar cane, sorghum, potato, cassava, Arabidopsis, tomato, tobacco, poplar, legume, and moss. Additional plant species can be bioinformatically assessed by contacting Invitrogen.

Working with something other than human, mouse, or plant? Contact us with your species and sequence. Simply include your FASTA sequence in *.txt file format for the ribosomal sequences you would like to verify, with the subject line titled "RiboMinus for (your organism here)", and we'll work with you to find the right probe for your RiboMinus® rRNA depletion.

RiboMinus™ Eukaryote System v2 (Invitrogen™)

The RiboMinus™ Eukaryote System v2 provides truly complete transcriptome isolation for next generation sequencing analysis by offering selective depletion of up to 99.9% of the cytoplasmic (5S, 5.8S, 18S, and 28S) and mitochondrial (12S and 16S) ribosomal RNA from total RNA. This selection system contrasts with poly-A and cap binding methods, which introduce bias and select for only part of the transcriptome.

Features of the RiboMinus™ Eukaryote System v2 include:

• Magnetic bead-based purification for rapid, seamless purification
• A 1-hour workflow
• An input range of 1–5 µg

The RiboMinus™ Eukaryote System v2 improves upon the previous system (RiboMinus™ Eukaryote Kit for RNA-Seq) by removing significantly more rRNA (both cytoplasmic and mitochondrial) from the total RNA. When used with our library construction kits for sequencing, the RiboMinus™ Eukaryote System v2 provides maximum mapping to reference database with minimum bias, maintaining relative gene expression levels of even smaller RNA species. The resulting total RNA after depletion contains minimum contaminating rRNA with reduced variability.

The RiboMinus Eukaryote System v2 is designed for the removal of human, mouse, and rat rRNA. A magnetic bead-based purification module is included for purification of the whole transcriptome without altering the expression levels of small or non-coding RNA. For column-based purification, please see the RiboMinus™ Eukaryote Kit v2. The RiboMinus Eukaryote System v2 has an RNA input range of 1–5 µg. For lower inputs (down to 100 ng), the Low Input RiboMinus™ Eukaryote System v2 is recommended.

The RiboMinus™ Eukaryote System v2 contains sufficient reagents to perform 12 ribosomal RNA depletions.

Ion AmpliSeq™ Direct FFPE DNA Kit (Ion Torrent™)

The Ion AmpliSeq™ Direct FFPE DNA Kit enables the preparation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for library construction using the Ion AmpliSeq protocol, without the need for deparaffinization or DNA purification. This kit is suitable for FFPE tissue sections up to 10 µm thick.

Features of the Ion AmpliSeq Direct FFPE DNA Kit include:
• Simple one-tube 2-step protocol that minimizes sample loss
• Efficient 30-minute workflow with only 10 minutes of hands-on time
• Ideal for FFPE DNA samples with limited DNA amount
• No deparaffinization of FFPE sections required
• No column or bead-based DNA purification required
• Ion AmpliSeq library preparation with as little as 1 ng input DNA
• Uracil-D-glycosylase treatment to remove deaminated cytosines is an optional step

Extraction of DNA from samples with limited DNA amount
Archived tissue samples can provide clinically relevant information, but it can be difficult to isolate the small amount of nucleic acid they contain. Deparaffinization of FFPE tissue sections and purification of the DNA in these samples can be a complicated, multi-step procedure requiring considerable hands-on time, and the associated sample-loss makes it an unsuitable approach for processing FFPE DNA samples with limited DNA amounts.

A simpler and faster method
The Ion AmpliSeq Direct FFPE DNA Kit provides a user-friendly protocol that eliminates the need for deparaffinization of FFPE tissue sections and purification of the DNA. From an FFPE section mounted on a glass slide, the area of interest is removed using Transfer Solution and pipetted into a PCR tube or 96-well plate. After the addition of Direct Reagent, the sample is incubated at 65°C for 15 minutes. The FFPE DNA is now ready for the Ion AmpliSeq™ Library Preparation protocol. The prepared FFPE DNA can be quantified using the Qubit™ HS DNA Quantitation Kit, if necessary. The simplicity of this protocol eliminates the need for complicated deparaffinization and DNA extraction procedures, and libraries can be prepared either manually or with the automated Ion Chef™ System.

RiboMinus™ Eukaryote Kit for RNA-Seq (Invitrogen™)

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:

• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.