Shop All General Immunoassay Buffers & Reagents

cAMP-Screen™ Wash Buffer Invitrogen™

This Wash Buffer is suitable for use with the cAMP-Screen® or cAMP-Screen Direct® Immunoassay system.

For Research Use Only. Not for use in diagnostics procedures.

cAMP-Screen™ Assay/Lysis Buffer Invitrogen™

This Assay⁄Lysis Buffer is suitable for use with the cAMP-Screen® or cAmp-Screen Direct® Immunoassay System.

For Research Use Only. Not for use in diagnostics procedures.

Normal Mouse Serum Invitrogen™

Normal Mouse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Pierce™ Protein-Free (TBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

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Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

HRP-Conjugate Diluent/Stabilizer Invitrogen™

This product may be used as a conjugate diluent in enzyme immunoassays.

eBioscience™ ELISA Wash Buffer, 1L packets Invitrogen™

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells. 10 packets of ELISA Wash Buffer Powder are supplied, yielding a total of 10 liters of 1x Wash Buffer.

Reported Application

Pierce™ Peroxidase Conjugate Stabilizer Thermo Scientific™

Thermo Scientific Pierce Peroxidase Conjugate Stabilizer is an antifreeze solution for storing freezer aliquots and stocks of antibodies and other HRP-conjugates in liquid form so they can be pipetted for use without thawing.

Features of Peroxidase Conjugate Stabilizer:

• Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins
• Contains high-purity ethylene glycol, which maintains a liquid, low viscosity solution at -20°C
• Contains preservatives to prevent anti-microbial growth during long-term storage
• No aliquoting of conjugate is necessary and sampling is convenient

Pierce Peroxidase Conjugate Stabilizer is a storage solution for peroxidase-conjugated antibodies, streptavidin and other proteins (HRP conjugates). Mixing the Stabilizer in 2:1 or 1:1 ratio with HRP conjugate solutions (usually at least 1 mg/mL) produces a stabilized conjugate stock solution that remains a liquid at -20°C. This allows the conjugate to be pipetted directly from the sub-zero temperature condition, eliminating damaging freeze-thaw cycles. The highly purified solution is free of oxidizing agents and other contaminants that can harm enzyme activity, ensuring that stock solutions can be stored for months without significant loss of activity.

Normal Mouse Serum Invitrogen™

Normal Mouse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Pierce™ Diethanolamine Substrate Buffer (5X) Thermo Scientific™

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection.

Features of Diethanolamine Substrate Buffer (5X):

• Ready-to-use
• Minimize assay-to-assay variability

Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP. Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.

Related Products
Pierce™ Stable Peroxide Substrate Buffer (10X)

Stop Solution for TMB Substrates Thermo Scientific™

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

Features of Stop Solution:

Size: 55 mL (sufficient for about five 96-well microplates)
Storage: Shipped at ambient temperature. Store at room temperature or 2-8°C.
Application: ELISA: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50–100 µL per well). Use at full strength.

Blocker™ BSA (10X) in PBS Thermo Scientific™

Blocker BSA (10X) is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.

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Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Related products
Blocker BSA (10X) in TBS

Stop Solution Invitrogen™

This stop solution is intended for use as a stop solution in enzyme assays which employ horseradish peroxidase and TMB substrate solution. It is suitable for use in microwell assays such as ELISA.

This product is For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Poly HRP Dilution Buffer Thermo Scientific™

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

InstantOne ELISA Cell Lysis Buffer (5X) Invitrogen™

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers.
Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits. The Cell Lysis Buffer (5X) contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Buffer (5X) is supplemented with Enhancer Solution to yield a versatile Cell Lysis Mix that can be applied to many cells and tissues. Please note the difference in names. Cell Lysis Mix is referred to heavily in the InstantOne phospho ELISA assay protocol.
A. The Cell Lysis Mix (5X) is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells.
B. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.
NOTE: *Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays.

Reported Application

Normal Rabbit Serum Invitrogen™

Normal Rabbit Serum Control for IF, ICC, IHC, BLOCK, Ctrl
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