Shop All General Immunoassay Buffers & Reagents

Pierce™ Peroxidase Conjugate Stabilizer Thermo Scientific™

Thermo Scientific Pierce Peroxidase Conjugate Stabilizer is an antifreeze solution for storing freezer aliquots and stocks of antibodies and other HRP-conjugates in liquid form so they can be pipetted for use without thawing.

Features of Peroxidase Conjugate Stabilizer:

• Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins
• Contains high-purity ethylene glycol, which maintains a liquid, low viscosity solution at -20°C
• Contains preservatives to prevent anti-microbial growth during long-term storage
• No aliquoting of conjugate is necessary and sampling is convenient

Pierce Peroxidase Conjugate Stabilizer is a storage solution for peroxidase-conjugated antibodies, streptavidin and other proteins (HRP conjugates). Mixing the Stabilizer in 2:1 or 1:1 ratio with HRP conjugate solutions (usually at least 1 mg/mL) produces a stabilized conjugate stock solution that remains a liquid at -20°C. This allows the conjugate to be pipetted directly from the sub-zero temperature condition, eliminating damaging freeze-thaw cycles. The highly purified solution is free of oxidizing agents and other contaminants that can harm enzyme activity, ensuring that stock solutions can be stored for months without significant loss of activity.

Blocker™ FL Fluorescent Blocking Buffer (10X)

Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications. Blocker FL buffer helps enhance fluorescent protein detection by increasing the signal-to-noise ratio in blotting applications where background fluorescence may otherwise interfere with signal detection.

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Working reagent is prepared by adding 1 part Blocker FL buffer to 9 parts ultrapure water. Optimal concentration should be determined empirically for each system. Compatible with both nitrocellulose and PVDF membranes.

Normal Rabbit Serum Invitrogen™

Normal Rabbit Serum Control for BLOCK, Ctrl

CellVue Diluent C (For CellVue™ Cell Labeling Kits) Invitrogen™

Diluent C is the Buffer required for the dilution of the dyes in the CellVue™ Cell Labeling Kits. Please refer to the specific labeling kit for instructions of use.

Reported Application
Flow Cytometric Analysis, Microscopy

Blocker™ Casein in PBS Thermo Scientific™

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

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Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

Related products
Blocker Casein in PBS

ELISA/ELISPOT Coating Buffer Powder Invitrogen™

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.

The eBioscience ELISA/ELISPOT Coating Buffer Powder is a dried powder formulation of phosphate buffered saline. Each packet is reconstituted in 1 Liter of distilled, deionized water, then filtered using a 0.22 um filter. The resulting 1x PBS (pH 7.4) is suitable for use as the ELISA/ELISPOT coating buffer.

Reported Application

Pierce™ Protein-Free (TBS) Blocking Buffer Thermo Scientific™

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

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Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

Wash Buffer (25X)

Wash buffer can be used for all ELISA assays.

InstantOne ELISA Cell Lysis Buffer (5X) Invitrogen™

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers.
Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits. The Cell Lysis Buffer (5X) contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Buffer (5X) is supplemented with Enhancer Solution to yield a versatile Cell Lysis Mix that can be applied to many cells and tissues. Please note the difference in names. Cell Lysis Mix is referred to heavily in the InstantOne phospho ELISA assay protocol.
A. The Cell Lysis Mix (5X) is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells.
B. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.
NOTE: *Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays.

Reported Application

SEA BLOCK Blocking Buffer Thermo Scientific™

Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Features of SEA BLOCK Blocking Buffer:

Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible—may be used for many different applications, including as a diluent for antibodies

Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.

Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.

Pierce™ Diethanolamine Substrate Buffer (5X) Thermo Scientific™

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection.

Features of Diethanolamine Substrate Buffer (5X):

• Ready-to-use
• Minimize assay-to-assay variability

Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP. Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.

Related Products
Pierce™ Stable Peroxide Substrate Buffer (10X)

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

SuperBlock™ (PBS) Blocking Buffer Thermo Scientific™

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

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Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

cAMP-Screen™ Wash Buffer Invitrogen™

This Wash Buffer is suitable for use with the cAMP-Screen® or cAMP-Screen Direct® Immunoassay system.

For Research Use Only. Not for use in diagnostics procedures.

ELISA Wash Buffer (30X) Thermo Scientific™

ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.

Features of the ELISA Wash Buffer:

Size: 50 mL (makes 1.5L of 1X buffer)
Storage: Shipped at on ice packs. Store at -20°C immediately upon receipt.
Application: Use as a wash buffer when performing ELISAs. This wash buffer is used in most, but not all, Thermo Scientific Pierce ELISA Kits. Please contact us for kit-specific information
Preparation: Thaw at room temperature (22-25°C). In a clean glass or plastic container, dilute the ELISA Wash Buffer concentrate 1:30 (vol:vol) with distilled or deionized water. Mix thoroughly. Equilibrate 1X wash buffer to room temperature before use in an assay. Prepare new 1X wash buffer for each day of use.
Automated Plate Washers: Storing the wash buffer in an automated system can result in contamination and poor ELISA performance. Automated plate washers must be cleaned regularly.
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