Shop All General Immunoassay Buffers & Reagents

QuantiGene™ Plex Wash Buffer Components (Invitrogen™)

QuantiGene Plex Wash Buffer Components are aqueous buffered solutions that are mixed with nuclease-free water to make QuantiGene Plex wash buffer. The wash buffer components may be purchased in 1, 3, or 10-plate quantities to supplement QuantiGene Plex Assay kits when additional wash buffer volume is required for automation.

Poly-HRP Dilution Buffer (Thermo Scientific™)

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

Normal Porcine Serum Control (Invitrogen™)

Normal Porcine Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Human Serum Control (Invitrogen™)

Normal Human Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Blocker™ FL Fluorescent Blocking Buffer (10X)

Blocker FL Fluorescent Blocking Buffer is designed to block excess nonspecific binding sites to help reduce background fluorescence in western blotting applications. Blocker FL buffer helps enhance fluorescent protein detection by increasing the signal-to-noise ratio in blotting applications where background fluorescence may otherwise interfere with signal detection.

Compare and view all available blocking buffers

Working reagent is prepared by adding 1 part Blocker FL buffer to 9 parts ultrapure water. Optimal concentration should be determined empirically for each system. Compatible with both nitrocellulose and PVDF membranes.

Normal Mouse Serum Control (Invitrogen™)

Normal Mouse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Poly HRP Dilution Buffer (Thermo Scientific™)

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

SuperBlock™ (PBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (PBS) Blocking Buffer - Blotting is optimized PBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (PBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

Stop Solution (Invitrogen™)

This stop solution is intended for use as a stop solution in enzyme assays which employ horseradish peroxidase and TMB substrate solution. It is suitable for use in microwell assays such as ELISA.

This product is For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Pierce™ Immunostain Enhancer (Thermo Scientific™)

Thermo Scientific Pierce Immunostain Enhancer enables significant antibody dilution (5- to 20-fold beyond normal) to increase signal intensity 3- to 12-fold in immunostaining applications with chromogenic or fluorescent substrates.

Features of Immunostain Enhancer:

Save precious antibody—Pierce Immunostain Enhancer allows the customer to use only a fraction of antibody to achieve the same signal as with conventional immunodetection
Convenience—simply replace your current antibody dilution buffer with Pierce Immunostain Enhancer (unlike other signal enhancement methods which require additional steps)
Increased signal intensity and sensitivity—provides 3- to 12- fold increase in signal intensity and sensitivity for improved visualization of the antigen of interest in cells and tissues
Improved specificity—significantly improves signal-to-noise ratio for poor quality and low affinity antibodies
Compatible—can be used with chromogenic and fluorescent detection methods

This ready-to-use enhancer for immunohistochemical staining does not add steps to the workflow because it is used to dilute the primary and secondary antibodies. The Pierce Immunostain Enhancer is compatible with fluorescence and chromogenic detection and routinely increases both signal intensity and detection sensitivity. Signal enhancement is antibody-dependent and typically ranges from 3- to 12-fold. Because of the strong signal enhancement, the Pierce Immunostain Enhancer reduces the amount of antibody required to achieve optimal detection.

The Pierce Immunostain Enhancer enables significant dilution of primary and secondary antibodies. Dilution 5- to 20-fold beyond the antibody vendor's recommendation has been used successfully; however, the dilution factor varies depending on the antibody, tissue, and antigen.

InstantOne ELISA Wash Buffer (10X) (Invitrogen™)

The InstantOne ELISA Wash Buffer (10X) is developed for use with the InstantOne ELISA Cell Signaling assays. The InstantOne ELISA Wash Buffer is supplied as a 10X concentrate and is used for washing the InstantOne ELISA cell signaling assay microplate. It is a simple mix of buffers, salts, and mild detergent. If washing wells with a microplate washer, use 3x washes with a 10 second mixing cycle.
The InstantOne ELISA Wash Buffer (10X) is included with every InstantOne ELISA sold. This wash buffer is offered additionally as a stand-alone item in cases where experimental procedures and/or automation require much higher volumes of wash buffer.

Reported Application
ELISA

SuperBlock™ (TBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

Normal Goat Serum Control (Invitrogen™)

Normal Goat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Wash Buffer (25X)

Wash buffer can be used for all ELISA assays.