Shop All General Immunoassay Buffers & Reagents

eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen™)

The IHC Antigen Retrieval Solution- High pH (10X) is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. Dilution of this buffer to 1X using reagent grade water results in a 10 mM Tris, 1 mM EDTA solution, pH 9.0.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

SuperBlock™ (PBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

CellVue Diluent C (For CellVue™ Cell Labeling Kits) (Invitrogen™)

Diluent C is the Buffer required for the dilution of the dyes in the CellVue™ Cell Labeling Kits. Please refer to the specific labeling kit for instructions of use.

Reported Application
Flow Cytometric Analysis, Microscopy

Amplex™ Red/UltraRed Stop Reagent (Invitrogen™)

Amplex® Red/UltraRed Stop Reagent is designed for use with the Amplex® Red and Amplex® UltraRed fluorogenic substrates and assay kits. Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.

See our complete line of Fluorescence Microplate assays.

Amplex® UltraRed and Amplex® Red assay kits are sensitive biomolecular assays based on hydrogen peroxide–generating enzyme systems linked to peroxidase–mediated oxidation of the fluorogenic Amplex® UltraRed or Amplex® Red substrates. Typically, detection reactions are performed in microplate wells and are initiated by adding the fluorogenic Amplex® UltraRed or Amplex® Red substrate, resulting in a continuous fluorescence increase that proceeds for 30 minutes or more. Ultimately, unknown analyte concentrations are determined by comparing fluorescence intensities measured at a certain time point during the reaction to parallel measurements at the same time point on standard samples of known concentration. Clearly, it is critical to ensure that the timing of the standard and unknown sample measurements is the same.

Amplex® Red/UltraRed Stop Reagent is designed for use with both the Amplex® Red and Amplex® UltraRed fluorogenic substrates and assay kits. It is designed to terminate reactions containing up to 0.1 units/mL of horseradish peroxidase (HRP) and 5 μM hyrogen peroxide. The fluorescent signal then remains stable for at least 3 hours.

InstantOne ELISA Cell Lysis Buffer (5X) (Invitrogen™)

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers.
Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits. The Cell Lysis Buffer (5X) contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Buffer (5X) is supplemented with Enhancer Solution to yield a versatile Cell Lysis Mix that can be applied to many cells and tissues. Please note the difference in names. Cell Lysis Mix is referred to heavily in the InstantOne phospho ELISA assay protocol.
A. The Cell Lysis Mix (5X) is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells.
B. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.
NOTE: *Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays.

Reported Application
ELISA

SuperBlock™ (TBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (TBS) Blocking Buffer - Blotting is optimized TBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (TBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

SEA BLOCK Blocking Buffer (Thermo Scientific™)

Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Features of SEA BLOCK Blocking Buffer:

Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible—may be used for many different applications, including as a diluent for antibodies

Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.

Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.

SuperBlock™ T20 (PBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

Normal Goat Serum (Invitrogen™)

Normal Goat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Pierce™ Protein-Free (TBS) Blocking Buffer (Thermo Scientific™)

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

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Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Normal Rat Serum (Invitrogen™)

Normal Rat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Enhancer Solution (Invitrogen™)

The InstantOne ELISA Enhancer Solution is a supplement added to the 5X Cell Lysis Buffer (Cat. No. IOCLB1) to yield the Cell Lysis Mix solution that is applied to cultured cells or tissues prior to running the assays. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with deionized water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.

Reported Application
ELISA

Stop Solution (Invitrogen™)

This stop solution is intended for use as a stop solution in enzyme assays which employ horseradish peroxidase and TMB substrate solution. It is suitable for use in microwell assays such as ELISA.

This product is For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Ethylene Glycol (Thermo Scientific™)

Thermo Scientific Pierce Ethylene Glycol Solution provides exceptional antifreeze protection and storage stability for antibody-enzyme conjugates because it is purified to remove impurities commonly found traditional glycerol stocks.

Features of Ethylene Glycol:

• Specially purified to remove impurities such as aldehydes, peroxides, iron and UV absorbing hydrocarbons
• Suitable for enzyme storage without the worry of losing enzymatic activity
• Stable for months

This product is a 50% (w/v) aqueous solution of highly purified ethylene glycol. When mixed in equal volume with purified protein samples, such as primary antibodies, the solution stabilizes and maintains the mixture as a liquid during freezer storage (-20°). Ethylene glycol is a suitable alternative to glycerol for most protein-storage applications. In fact, this preparation of ethylene glycol is devoid of various common glycerol impurities and potiential degradation products that are damaging to certain enzymes.

StartingBlock™ (PBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

Compare and view all available blocking buffers

Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Support Pack Plus (Invitrogen™)

Buffer pack for 10 plates (plates included)

Reactivity/Species
Human, Mouse, Rat

Reported Application
ELISA

Blocker™ BSA (10X) in TBS (Thermo Scientific™)

Blocker BSA (10X) is purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC and nucleic acid detection methods.

Compare and view all available blocking buffers

Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Related products
Blocker BSA (10X) in PBS

Blocker™ Casein in PBS (Thermo Scientific™)

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Compare and view all available blocking buffers

Features:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

Related products
Blocker Casein in TBS

SuperBlock™ (PBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (PBS) Blocking Buffer - Blotting is optimized PBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (PBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

eBioscience™ ELISA Wash Buffer, 1L packets (Invitrogen™)

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells. 10 packets of ELISA Wash Buffer Powder are supplied, yielding a total of 10 liters of 1x Wash Buffer.

Reported Application
ELISA

Pierce™ Immunostain Enhancer (Thermo Scientific™)

Thermo Scientific Pierce Immunostain Enhancer enables significant antibody dilution (5- to 20-fold beyond normal) to increase signal intensity 3- to 12-fold in immunostaining applications with chromogenic or fluorescent substrates.

Features of Immunostain Enhancer:

Save precious antibody—Pierce Immunostain Enhancer allows the customer to use only a fraction of antibody to achieve the same signal as with conventional immunodetection
Convenience—simply replace your current antibody dilution buffer with Pierce Immunostain Enhancer (unlike other signal enhancement methods which require additional steps)
Increased signal intensity and sensitivity—provides 3- to 12- fold increase in signal intensity and sensitivity for improved visualization of the antigen of interest in cells and tissues
Improved specificity—significantly improves signal-to-noise ratio for poor quality and low affinity antibodies
Compatible—can be used with chromogenic and fluorescent detection methods

This ready-to-use enhancer for immunohistochemical staining does not add steps to the workflow because it is used to dilute the primary and secondary antibodies. The Pierce Immunostain Enhancer is compatible with fluorescence and chromogenic detection and routinely increases both signal intensity and detection sensitivity. Signal enhancement is antibody-dependent and typically ranges from 3- to 12-fold. Because of the strong signal enhancement, the Pierce Immunostain Enhancer reduces the amount of antibody required to achieve optimal detection.

The Pierce Immunostain Enhancer enables significant dilution of primary and secondary antibodies. Dilution 5- to 20-fold beyond the antibody vendor's recommendation has been used successfully; however, the dilution factor varies depending on the antibody, tissue, and antigen.

ELISA Wash Buffer (30X) (Thermo Scientific™)

ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.

Features of the ELISA Wash Buffer:

Size: 50 mL (makes 1.5L of 1X buffer)
Storage: Shipped at on ice packs. Store at -20°C immediately upon receipt.
Application: Use as a wash buffer when performing ELISAs. This wash buffer is used in most, but not all, Thermo Scientific Pierce ELISA Kits. Please contact us for kit-specific information
Preparation: Thaw at room temperature (22-25°C). In a clean glass or plastic container, dilute the ELISA Wash Buffer concentrate 1:30 (vol:vol) with distilled or deionized water. Mix thoroughly. Equilibrate 1X wash buffer to room temperature before use in an assay. Prepare new 1X wash buffer for each day of use.
Automated Plate Washers: Storing the wash buffer in an automated system can result in contamination and poor ELISA performance. Automated plate washers must be cleaned regularly.

I-Block™ Protein-Based Blocking Reagent (Invitrogen™)

I-Block reagent is a highly purified casein-based blocking reagent. It provides superior blocking compared to both dried milk and BSA. Unlike other casein-based blocking reagents, I-Block reagent is essentially biotin-free. I-Block reagent is tested in assays using Tropix substrates and alkaline phosphatase conjugates, and is useful as a blocking reagent in membrane-based and immunoassay applications.

The suggested working concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon). For protein detection on positively-charged nylon membrane (such as TropilonPlus membrane), a concentration of 3% is recommended. I-Block blocking solution is prepared in either Tris- or phosphate-buffered saline buffer with heating (40-50°C).

This casein-based blocking reagent can be used with Western-Light, Western-Light Plus, Western-Star, ELISA-Light, Southern-Light, and Southern-Star detection systems.

Coating Buffer A

The Invitrogen Coating Buffer A is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Normal Porcine Serum (Invitrogen™)

Normal Porcine Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Miser™ Antibody Extender Solution NC (Thermo Scientific™)

Thermo Scientific Pierce Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Features of Antibody Extender Solution NC:

Achieve equivalent signal—use at least three times less primary antibody than normal and retain comparable or better signal detectability
Any detection mode or enzyme—the method works with both colorimetric and chemiluminescent detection modes and with horseradish peroxidase (HRP) and alkaline phosphatase (AP) systems
Easy-to-perform, 10-minute protocol—invest a few minutes and save money on your primary antibody; our scientists have reduced their use of expensive primary antibodies by up to 100-fold with this product

By treating nitrocellulose membranes for a few minutes in this reagent immediately after transfer and before probing, you can use much less (more dilute) primary antibody solutions while obtaining the same level of detection. This conserves use of expensive and rare antibodies, enabling more experiments to be performed with the same amount of antibody. The reagent is so effective that our scientists have observed antibody cost reductions of 10-, 25- and even 100-times (depending on the antigen and primary antibody directed against that antigen).

Poly HRP Dilution Buffer (Thermo Scientific™)

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

Peroxidase Suppressor (Thermo Scientific™)

Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.

Features of Peroxidase Suppressor:

• Stable
• One component
• Ready-to-use
• Effectively inhibits peroxidase activity without interfering with antigen-antibody
interactions

This peroxidase suppressor is optimized for immunohistochemical applications. The Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity. This proprietary formulation is the most effective peroxidase inhibitor available. The immunoperoxidase method is a commonly used detection method for immunostaining of cells and tissues. However, the analysis often is complicated by the presence of endogenous peroxidase and "pseudoperoxidase" activity in the cells and tissues. This presence results in nonspecific staining when a substrate such as diaminobenzidine (DAB) is present. Nonspecific staining is difficult to distinguish from the immunochemical marker, making it difficult to distinguish the final location of the antigen of interest. The typical method of inhibiting endogenous peroxidase activity involves hydrogen peroxide or hydrogen peroxide in methanol. The peroxidase suppressor inhibits peroxidase activity even more effectively than the method using hydrogen peroxide in methanol. Plus, the Peroxidase Suppressor does not affect antibody activity (see figure). Peroxidase Suppressor also can be used on blots of whole cell supernatant prior to the addition of the horseradish peroxidase (HRP) marker.

Pierce™ Fast Blocking Buffer (Thermo Scientific™)

Pierce Fast Blocking Buffer effectively and reliably blocks western blots in just five minutes to provide low-background results for traditional western blotting protocols.

Compare and view all available blocking buffers

Pierce Fast Blocking Buffer streamlines western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only five minutes, producing results comparable to traditional blockers. Blocking and probing steps with Fast Blocking Buffer prevent nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). This buffer is compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes.

Related products
Pierce Fast Wash Buffer, 10X