Shop All General Immunoassay Buffers & Reagents

Pierce™ Diethanolamine Substrate Buffer (5X) (Thermo Scientific™)

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection.

Features of Diethanolamine Substrate Buffer (5X):

• Ready-to-use
• Minimize assay-to-assay variability

Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP. Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.

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Pierce™ Stable Peroxide Substrate Buffer (10X)

StartingBlock™ (PBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

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Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Pierce™ Clear Milk Blocking Buffer (10X) (Thermo Scientific™)

Pierce Clear Milk Blocking Buffer (10X) is a long-shelf life, high-performance replacement for homemade non-fat milk blocking buffers in western blotting applications.

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Our Clear Milk Blocking Buffer (10X) is a pre-formulated milk solution, clarified and stabilized for blocking excess nonspecific binding sites, reducing background in western blotting applications and diluting antibodies when used with nitrocellulose and PVDF membranes. It provides lower background, enhanced sensitivity, extended shelf life, and reproducible results compared to homemade buffers based on dissolved non-fat dry milk. Not recommended for avidin-based techniques because it contains some endogenous biotin.

Stop Solution (Invitrogen™)

This stop solution is intended for use as a stop solution in enzyme assays which employ horseradish peroxidase and TMB substrate solution. It is suitable for use in microwell assays such as ELISA.

This product is For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Ethylene Glycol (Thermo Scientific™)

Thermo Scientific Pierce Ethylene Glycol Solution provides exceptional antifreeze protection and storage stability for antibody-enzyme conjugates because it is purified to remove impurities commonly found traditional glycerol stocks.

Features of Ethylene Glycol:

• Specially purified to remove impurities such as aldehydes, peroxides, iron and UV absorbing hydrocarbons
• Suitable for enzyme storage without the worry of losing enzymatic activity
• Stable for months

This product is a 50% (w/v) aqueous solution of highly purified ethylene glycol. When mixed in equal volume with purified protein samples, such as primary antibodies, the solution stabilizes and maintains the mixture as a liquid during freezer storage (-20°). Ethylene glycol is a suitable alternative to glycerol for most protein-storage applications. In fact, this preparation of ethylene glycol is devoid of various common glycerol impurities and potiential degradation products that are damaging to certain enzymes.

Enhancer Solution (Invitrogen™)

The InstantOne ELISA Enhancer Solution is a supplement added to the 5X Cell Lysis Buffer (Cat. No. IOCLB1) to yield the Cell Lysis Mix solution that is applied to cultured cells or tissues prior to running the assays. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with deionized water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.

Reported Application
ELISA

SuperBlock™ T20 (PBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

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Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

Assay Buffer (5X) (Invitrogen™)

Assay Buffer (5x) for Antibody Pairs is intended to be used as a:(1) blocking reagent for ELISA plates(2) diluent for ELISA standards and samples(3) diluent for the detection antibody(4) diluent for the HRP conjugate

Application: Antibody pairs (ELISA)

StartingBlock™ (TBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

Compare and view all available blocking buffers

Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock (PBS) Blocking Buffer
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Pierce™ Immunostain Enhancer (Thermo Scientific™)

Thermo Scientific Pierce Immunostain Enhancer enables significant antibody dilution (5- to 20-fold beyond normal) to increase signal intensity 3- to 12-fold in immunostaining applications with chromogenic or fluorescent substrates.

Features of Immunostain Enhancer:

Save precious antibody—Pierce Immunostain Enhancer allows the customer to use only a fraction of antibody to achieve the same signal as with conventional immunodetection
Convenience—simply replace your current antibody dilution buffer with Pierce Immunostain Enhancer (unlike other signal enhancement methods which require additional steps)
Increased signal intensity and sensitivity—provides 3- to 12- fold increase in signal intensity and sensitivity for improved visualization of the antigen of interest in cells and tissues
Improved specificity—significantly improves signal-to-noise ratio for poor quality and low affinity antibodies
Compatible—can be used with chromogenic and fluorescent detection methods

This ready-to-use enhancer for immunohistochemical staining does not add steps to the workflow because it is used to dilute the primary and secondary antibodies. The Pierce Immunostain Enhancer is compatible with fluorescence and chromogenic detection and routinely increases both signal intensity and detection sensitivity. Signal enhancement is antibody-dependent and typically ranges from 3- to 12-fold. Because of the strong signal enhancement, the Pierce Immunostain Enhancer reduces the amount of antibody required to achieve optimal detection.

The Pierce Immunostain Enhancer enables significant dilution of primary and secondary antibodies. Dilution 5- to 20-fold beyond the antibody vendor's recommendation has been used successfully; however, the dilution factor varies depending on the antibody, tissue, and antigen.

Blocker™ Casein in TBS (Thermo Scientific™)

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

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Features:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

Related products
Blocker Casein in PBS

ELISA/ELISPOT Coating Buffer Powder (Invitrogen™)

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.

The eBioscience ELISA/ELISPOT Coating Buffer Powder is a dried powder formulation of phosphate buffered saline. Each packet is reconstituted in 1 Liter of distilled, deionized water, then filtered using a 0.22 um filter. The resulting 1x PBS (pH 7.4) is suitable for use as the ELISA/ELISPOT coating buffer.

Reported Application
ELISA, ELISPOT

ELISA Blocker Blocking Buffer (Thermo Scientific™)

ELISA Blocker Blocking Buffer is a protein buffer that effectively blocks plate binding sites that remain in polystyrene microplate wells following initial coating steps in ELISA procedures. The ready-to-use buffer is optimized and routinely used for sandwich ELISA procedures involving cytokines, such as many of the Thermo Scientific Pierce ELISA Kits.

Features OF THE ELISA Blocking Buffer:

Size: 1L (sufficient for thirty 96-well plates)
Application: Use as a blocking buffer in ELISA. The buffer is ready to use. No dilution is required.
Instructions for use in an ELISA: Following the incubation with a coating antibody, aspirate the coating antibody from the plate and add 300 µL of ELISA Blocker Blocking Buffer to each well. Cover the plate with a plate sealer and incubate for a minimum of 1 hour at room temperature (22-25°C). Aspirate blocking buffer and proceed with the ELISA procedure or dry the plate for future use.
Plate Drying: Plates should be dried uncovered, at room temperature, for a minimum of 1 hour. For optimal drying, plates should not be stacked and humidity should be at 30-40%. If plates are stacked, or humidity is high, the drying time should be increased. Once drying is complete, seal each plate in a foil pouch with a desiccant. When sealed with a desiccant, plates may be stored at 2-8°C for up to 6 months.

eBioscience™ IHC/ICC Blocking Buffer - High Protein (Invitrogen™)

The IHC/ICC Blocking Buffer- High Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with high background staining, nuclear antigens, and FFPE tissue. This blocking buffer can be used both in the blocking step and as a diluent for primary and secondary antibodies during antibody incubation steps. The IHC/ICC Blocking Buffer-High Protein is compatible with organic dye-conjugated antibodies as well as unconjugated antibodies that may be used in two and three step staining protocols. This buffer is supplied as a 1X stock solution.

Reported Application
Immunohistochemical Staining, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

eBioscience™ 20X TBS Wash Buffer for IHC/ICC (Invitrogen™)

The 20X TBS Wash Buffer for IHC/ICC is for use in immunohistochemistry and immunocytochemistry protocols which require washing to remove non-specific binding. This wash buffer is recommended for eFluor™ Nanocrystal conjugated-antibodies following antibody incubation. The TBS Wash Buffer is also compatible with organic dye-conjugated antibodies as well as unconjugated antibodies that may be used in two and three step staining protocols. The buffer is supplied as a 20X stock solution that should be diluted to 1X with distilled water.

Reported Application
Immunohistochemical Staining, Immunocytochemistry