Shop All General Immunoassay Buffers & Reagents

SEA BLOCK Blocking Buffer (Thermo Scientific™)

SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC, fluorescent western blotting, and other detection methods involving mammalian samples.

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Features of SEA BLOCK Blocking Buffer:
Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed

Fish serum has several advantages over typical blocking buffers. Salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, and its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. It is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal serum is causing too much background in immunohistochemistry (IHC), try this product.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. It has been used to decrease background and increase signal-to-noise ratios in fluorescent western blotting and in various cellular imaging protocols.

Miser™ Antibody Extender Solution NC (Thermo Scientific™)

Thermo Scientific Pierce Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Features of Antibody Extender Solution NC:

Achieve equivalent signal—use at least three times less primary antibody than normal and retain comparable or better signal detectability
Any detection mode or enzyme—the method works with both colorimetric and chemiluminescent detection modes and with horseradish peroxidase (HRP) and alkaline phosphatase (AP) systems
Easy-to-perform, 10-minute protocol—invest a few minutes and save money on your primary antibody; our scientists have reduced their use of expensive primary antibodies by up to 100-fold with this product

By treating nitrocellulose membranes for a few minutes in this reagent immediately after transfer and before probing, you can use much less (more dilute) primary antibody solutions while obtaining the same level of detection. This conserves use of expensive and rare antibodies, enabling more experiments to be performed with the same amount of antibody. The reagent is so effective that our scientists have observed antibody cost reductions of 10-, 25- and even 100-times (depending on the antigen and primary antibody directed against that antigen).

Support Pack Plus (Invitrogen™)

Buffer pack for 10 plates (plates included)

Human, Mouse, Rat

Reported Application

QuantiGene™ Plex Wash Buffer Components (Invitrogen™)

QuantiGene Plex Wash Buffer Components are aqueous buffered solutions that are mixed with nuclease-free water to make QuantiGene Plex wash buffer. The wash buffer components may be purchased in 1, 3, or 10-plate quantities to supplement QuantiGene Plex Assay kits when additional wash buffer volume is required for automation.

InstantOne ELISA Wash Buffer (10X) (Invitrogen™)

The InstantOne ELISA Wash Buffer (10X) is developed for use with the InstantOne ELISA Cell Signaling assays. The InstantOne ELISA Wash Buffer is supplied as a 10X concentrate and is used for washing the InstantOne ELISA cell signaling assay microplate. It is a simple mix of buffers, salts, and mild detergent. If washing wells with a microplate washer, use 3x washes with a 10 second mixing cycle.
The InstantOne ELISA Wash Buffer (10X) is included with every InstantOne ELISA sold. This wash buffer is offered additionally as a stand-alone item in cases where experimental procedures and/or automation require much higher volumes of wash buffer.

Reported Application

eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen™)

The IHC Antigen Retrieval Solution- High pH (10X) is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. Dilution of this buffer to 1X using reagent grade water results in a 10 mM Tris, 1 mM EDTA solution, pH 9.0.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

Stop Solution for TMB Substrates (Thermo Scientific™)

Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3’,5,5’ - tetramethylbenzidine (TMB). In the presence of horseradish peroxidase (HRP) enzyme conjugates, TMB and peroxide react to produce a blue byproduct having maximum absorbance at 605nm. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA procedure. Addition of sulfuric acid stop solution changes the color to yellow (absorbance maximum at 450nm), stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

Features of Stop Solution:

Size: 55 mL (sufficient for about five 96-well microplates)
Storage: Shipped at ambient temperature. Store at room temperature or 2-8°C.
Application: ELISA: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50–100 µL per well). Use at full strength.

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

HRP-Conjugate Diluent/Stabilizer (Invitrogen™)

This product may be used as a conjugate diluent in enzyme immunoassays.

SuperBlock™ (PBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (PBS) Blocking Buffer - Blotting is optimized PBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (PBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

Normal Horse Serum (Invitrogen™)

Normal Horse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

StartingBlock™ T20 (TBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock is available in PBS and TBS with and without Tween20.

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Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock (PBS) Blocking Buffer
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer

Pierce™ Protein-Free T20 (TBS) Blocking Buffer (Thermo Scientific™)

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

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Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer

Normal Rabbit Serum (Invitrogen™)

Normal Rabbit Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Rat Serum (Invitrogen™)

Normal Rat Serum Control for IF, ICC, IHC, BLOCK, Ctrl