Shop All General Immunoassay Buffers & Reagents

Poly-HRP Dilution Buffer (Thermo Scientific™)

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

Buffer Kit for Antibody Pairs (Invitrogen™)

This Buffer Kit for Antibody Pairs is for use with Invitrogen Antibody Pairs. This Kit provides sufficient materials for 10 ELISA plates.

StartingBlock™ T20 (TBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock is available in PBS and TBS with and without Tween20.

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Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock (PBS) Blocking Buffer
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer

SuperBlock™ (TBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

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Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

Support Pack Plus (Invitrogen™)

Buffer pack for 10 plates (plates included)

Human, Mouse, Rat

Reported Application

Protein Stabilizing Cocktail (Thermo Scientific™)

The Thermo Scientific Protein Stabilizing Cocktail is a versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

Features of the Protein Stabilizing Cocktail:

• Stabilizes most enzymes and other proteins significantly better than ordinary buffers
• Protect proteins from environmental stresses
• Does not destabilize biomolecules in downstream assays
• Components are low molecular weight and fully dialyzable
• Low-viscosity reagent is easier to pipette than 50% glycerol
• Protein classes tested include kinases, phosphatases, peroxidases, restriction enzymes, luciferases, cytokines and antibodies

This proprietary formulation of low-molecular weight, naturally occurring molecules helps protect proteins from environmental stresses that can otherwise lead to enzyme inactivation, aggregation and freeze-thaw damage. The Protein Stabilizing Cocktail is provided as an easily pipettable, buffered 4X concentrate. Solutions of enzymes and other proteins to which the cocktail has been added may be refrigerated or frozen for storage without losing their activity or function. Although the degree of stabilization is protein-specific, the cocktail significantly stabilizes most proteins compared with conventional buffer alone. The Protein Stabilizing Cocktail is nontoxic and does not destabilize biomolecules; however, all cocktail components can be removed by dialysis or desalting before use in downstream assays, if desired.

eBioscience™ IHC /ICC Blocking Buffer - Low Protein (Invitrogen™)

The IHC/ICC Blocking Buffer- Low Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with average levels of background staining. This blocking buffer can be used both in the blocking step and as a diluent for unconjugated antibodies and organic dye-conjugated antibodies during the antibody incubation step. This buffer is supplied as a 1X stock solution containing sodium azide.

Reported Application
Immunohistochemical Staining, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

SEA BLOCK Blocking Buffer (Thermo Scientific™)

Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Features of SEA BLOCK Blocking Buffer:

Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible—may be used for many different applications, including as a diluent for antibodies

Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.

Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.

Enhancer Solution (Invitrogen™)

The InstantOne ELISA Enhancer Solution is a supplement added to the 5X Cell Lysis Buffer (Cat. No. IOCLB1) to yield the Cell Lysis Mix solution that is applied to cultured cells or tissues prior to running the assays. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with deionized water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.

Reported Application

eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Invitrogen™)

The IHC Antigen Retrieval Solution- Low pH (10X) is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. Dilution of this buffer to 1X using reagent grade water results in a 10 mM Sodium Citrate solution, pH 6.0.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

Pierce™ Peroxidase Conjugate Stabilizer (Thermo Scientific™)

Thermo Scientific Pierce Peroxidase Conjugate Stabilizer is an antifreeze solution for storing freezer aliquots and stocks of antibodies and other HRP-conjugates in liquid form so they can be pipetted for use without thawing.

Features of Peroxidase Conjugate Stabilizer:

• Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins
• Contains high-purity ethylene glycol, which maintains a liquid, low viscosity solution at -20°C
• Contains preservatives to prevent anti-microbial growth during long-term storage
• No aliquoting of conjugate is necessary and sampling is convenient

Pierce Peroxidase Conjugate Stabilizer is a storage solution for peroxidase-conjugated antibodies, streptavidin and other proteins (HRP conjugates). Mixing the Stabilizer in 2:1 or 1:1 ratio with HRP conjugate solutions (usually at least 1 mg/mL) produces a stabilized conjugate stock solution that remains a liquid at -20°C. This allows the conjugate to be pipetted directly from the sub-zero temperature condition, eliminating damaging freeze-thaw cycles. The highly purified solution is free of oxidizing agents and other contaminants that can harm enzyme activity, ensuring that stock solutions can be stored for months without significant loss of activity.

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Normal Rabbit Serum (Invitrogen™)

Normal Rabbit Serum Control for BLOCK, Ctrl

Normal Porcine Serum (Invitrogen™)

Normal Porcine Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Assay Buffer (10X) (Invitrogen™)

This Assay Buffer is used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix® substrate conjugates.

For Research Use Only. Not for use in diagnostics procedures.