Shop All General Immunoassay Buffers & Reagents

eBioscience™ ELISA Wash Buffer, 1L packets (Invitrogen™)

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells. 10 packets of ELISA Wash Buffer Powder are supplied, yielding a total of 10 liters of 1x Wash Buffer.

Reported Application
ELISA

Protein Stabilizing Cocktail (Thermo Scientific™)

The Thermo Scientific Protein Stabilizing Cocktail is a versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

Features of the Protein Stabilizing Cocktail:

• Stabilizes most enzymes and other proteins significantly better than ordinary buffers
• Protect proteins from environmental stresses
• Does not destabilize biomolecules in downstream assays
• Components are low molecular weight and fully dialyzable
• Low-viscosity reagent is easier to pipette than 50% glycerol
• Protein classes tested include kinases, phosphatases, peroxidases, restriction enzymes, luciferases, cytokines and antibodies

This proprietary formulation of low-molecular weight, naturally occurring molecules helps protect proteins from environmental stresses that can otherwise lead to enzyme inactivation, aggregation and freeze-thaw damage. The Protein Stabilizing Cocktail is provided as an easily pipettable, buffered 4X concentrate. Solutions of enzymes and other proteins to which the cocktail has been added may be refrigerated or frozen for storage without losing their activity or function. Although the degree of stabilization is protein-specific, the cocktail significantly stabilizes most proteins compared with conventional buffer alone. The Protein Stabilizing Cocktail is nontoxic and does not destabilize biomolecules; however, all cocktail components can be removed by dialysis or desalting before use in downstream assays, if desired.

StartingBlock™ T20 (PBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

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Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Coating Buffer A

The Invitrogen Coating Buffer A is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

cAMP-Screen™ Wash Buffer (Invitrogen™)

This Wash Buffer is suitable for use with the cAMP-Screen® or cAMP-Screen Direct® Immunoassay system.

For Research Use Only. Not for use in diagnostics procedures.

Normal Mouse Serum (Invitrogen™)

Normal Mouse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

cAMP-Screen™ Assay/Lysis Buffer (Invitrogen™)

This Assay⁄Lysis Buffer is suitable for use with the cAMP-Screen® or cAmp-Screen Direct® Immunoassay System.

For Research Use Only. Not for use in diagnostics procedures.

Blocker™ BSA (10X) in TBS (Thermo Scientific™)

Blocker BSA (10X) is purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC and nucleic acid detection methods.

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Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Related products
Blocker BSA (10X) in PBS

Pierce™ Peroxidase Conjugate Stabilizer (Thermo Scientific™)

Thermo Scientific Pierce Peroxidase Conjugate Stabilizer is an antifreeze solution for storing freezer aliquots and stocks of antibodies and other HRP-conjugates in liquid form so they can be pipetted for use without thawing.

Features of Peroxidase Conjugate Stabilizer:

• Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins
• Contains high-purity ethylene glycol, which maintains a liquid, low viscosity solution at -20°C
• Contains preservatives to prevent anti-microbial growth during long-term storage
• No aliquoting of conjugate is necessary and sampling is convenient

Pierce Peroxidase Conjugate Stabilizer is a storage solution for peroxidase-conjugated antibodies, streptavidin and other proteins (HRP conjugates). Mixing the Stabilizer in 2:1 or 1:1 ratio with HRP conjugate solutions (usually at least 1 mg/mL) produces a stabilized conjugate stock solution that remains a liquid at -20°C. This allows the conjugate to be pipetted directly from the sub-zero temperature condition, eliminating damaging freeze-thaw cycles. The highly purified solution is free of oxidizing agents and other contaminants that can harm enzyme activity, ensuring that stock solutions can be stored for months without significant loss of activity.

eBioscience™ ELISA/ELISPOT Diluent (5X) (Invitrogen™)

The 5X ELISA/ELISPOT Diluent is a Phosphate Buffered Solution supplemented with fetal bovine serum as a source of animal serum proteins. This buffer is used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples, and making the working dilutions of the secondary detection reagent (i.e., detection antibody-biotin and AV-HRP Cat. No. 18-4100). This diluent solution also contains a preservative.

Reported Application
ELISA

SuperBlock™ (TBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (TBS) Blocking Buffer - Blotting is optimized TBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (TBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

Blocker™ BLOTTO in TBS (Thermo Scientific™)

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

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Features of Blocker BLOTTO Blocking Buffer:
Format—ready-to-use 1X TBS solution; can be diluted as needed
Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

SuperBlock™ T20 (TBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

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Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

Normal Human Serum (Invitrogen™)

Normal Human Serum Control for IF, ICC, IHC, BLOCK, Ctrl