Shop All General Immunoassay Buffers & Reagents

Pierce™ Immunostain Enhancer (Thermo Scientific™)

Thermo Scientific Pierce Immunostain Enhancer enables significant antibody dilution (5- to 20-fold beyond normal) to increase signal intensity 3- to 12-fold in immunostaining applications with chromogenic or fluorescent substrates.

Features of Immunostain Enhancer:

Save precious antibody—Pierce Immunostain Enhancer allows the customer to use only a fraction of antibody to achieve the same signal as with conventional immunodetection
Convenience—simply replace your current antibody dilution buffer with Pierce Immunostain Enhancer (unlike other signal enhancement methods which require additional steps)
Increased signal intensity and sensitivity—provides 3- to 12- fold increase in signal intensity and sensitivity for improved visualization of the antigen of interest in cells and tissues
Improved specificity—significantly improves signal-to-noise ratio for poor quality and low affinity antibodies
Compatible—can be used with chromogenic and fluorescent detection methods

This ready-to-use enhancer for immunohistochemical staining does not add steps to the workflow because it is used to dilute the primary and secondary antibodies. The Pierce Immunostain Enhancer is compatible with fluorescence and chromogenic detection and routinely increases both signal intensity and detection sensitivity. Signal enhancement is antibody-dependent and typically ranges from 3- to 12-fold. Because of the strong signal enhancement, the Pierce Immunostain Enhancer reduces the amount of antibody required to achieve optimal detection.

The Pierce Immunostain Enhancer enables significant dilution of primary and secondary antibodies. Dilution 5- to 20-fold beyond the antibody vendor's recommendation has been used successfully; however, the dilution factor varies depending on the antibody, tissue, and antigen.

StartingBlock™ (PBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

Compare and view all available blocking buffers

Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock T20 (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Blocker™ BSA (10X) in PBS (Thermo Scientific™)

Blocker BSA (10X) is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.

Compare and view all available blocking buffers

Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. It is most frequently diluted 10-fold (to 1% BSA) in 1X PBS or 1X TBS for initial testing. Blocker BSA is usually more effective than nonfat milk for biotin-avidin systems because it contains a single purified protein that is devoid of endogenous biotin.

Related products
Blocker BSA (10X) in TBS

Normal Rabbit Serum (Invitrogen™)

Normal Rabbit Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Human Serum (Invitrogen™)

Normal Human Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Rat Serum (Invitrogen™)

Normal Rat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Coating Buffer A

The Invitrogen Coating Buffer A is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Pierce™ Protein-Free T20 (PBS) Blocking Buffer (Thermo Scientific™)

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

Compare and view all available blocking buffers

Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer
Pierce Protein-Free T20 (TBS) Blocking Buffer

Protein Stabilizing Cocktail (Thermo Scientific™)

The Thermo Scientific Protein Stabilizing Cocktail is a versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.

Features of the Protein Stabilizing Cocktail:

• Stabilizes most enzymes and other proteins significantly better than ordinary buffers
• Protect proteins from environmental stresses
• Does not destabilize biomolecules in downstream assays
• Components are low molecular weight and fully dialyzable
• Low-viscosity reagent is easier to pipette than 50% glycerol
• Protein classes tested include kinases, phosphatases, peroxidases, restriction enzymes, luciferases, cytokines and antibodies

This proprietary formulation of low-molecular weight, naturally occurring molecules helps protect proteins from environmental stresses that can otherwise lead to enzyme inactivation, aggregation and freeze-thaw damage. The Protein Stabilizing Cocktail is provided as an easily pipettable, buffered 4X concentrate. Solutions of enzymes and other proteins to which the cocktail has been added may be refrigerated or frozen for storage without losing their activity or function. Although the degree of stabilization is protein-specific, the cocktail significantly stabilizes most proteins compared with conventional buffer alone. The Protein Stabilizing Cocktail is nontoxic and does not destabilize biomolecules; however, all cocktail components can be removed by dialysis or desalting before use in downstream assays, if desired.

ELISA Wash Buffer (30X) (Thermo Scientific™)

ELISA Wash Buffer is a Tris-based wash buffer for use in cytokine and other sandwich ELISA procedures. The buffer is the same formulation that is supplied with most Thermo Scientific Pierce ELISA Kits for cytokines and chemokines. The 30X concentrated stock solution is easily diluted 1:30 (vol:vol) in ultrapure water to yield sufficient wash buffer for a typical 96-well microplate ELISA protocol. Supplement an existing ELISA Kit or use as a convenient general purpose wash buffer for customized ELISA methods.

Features of the ELISA Wash Buffer:

Size: 50 mL (makes 1.5L of 1X buffer)
Storage: Shipped at on ice packs. Store at -20°C immediately upon receipt.
Application: Use as a wash buffer when performing ELISAs. This wash buffer is used in most, but not all, Thermo Scientific Pierce ELISA Kits. Please contact us for kit-specific information
Preparation: Thaw at room temperature (22-25°C). In a clean glass or plastic container, dilute the ELISA Wash Buffer concentrate 1:30 (vol:vol) with distilled or deionized water. Mix thoroughly. Equilibrate 1X wash buffer to room temperature before use in an assay. Prepare new 1X wash buffer for each day of use.
Automated Plate Washers: Storing the wash buffer in an automated system can result in contamination and poor ELISA performance. Automated plate washers must be cleaned regularly.

Blocker™ Casein in TBS (Thermo Scientific™)

Blocker Casein is a ready-to-use solution of purified casein for blocking steps in western blot, ELISA, IHC, and nucleic acid detection methods. Blocker Casein is 1% (w/v) casein, which corresponds to the optimal concentration for most applications.

Compare and view all available blocking buffers

Features:
Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use—1% casein solution is ready to use; can be diluted further as needed
Safe—stable, thimerosal-free formulation

Related products
Blocker Casein in PBS

Normal Mouse Serum (Invitrogen™)

Normal Mouse Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Normal Goat Serum (Invitrogen™)

Normal Goat Serum Control for IF, ICC, IHC, BLOCK, Ctrl

Poly HRP Dilution Buffer (Thermo Scientific™)

Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.

Features of Poly-HRP Dilution Buffer:

Formulation: 1% biotin-free casein in a PBS buffer solution.
Application: Dilution of Poly-HRP Streptavidin (Product # N200): Gently invert bottle and swirl to mix before use. Do not vortex. Use at full strength. Refer to Poly-HRP Streptavidin Certificate of Analysis for recommended dilution ranges. Dilute Poly-HRP Streptavidin immediately before use.

Related Products
Poly-HRP Streptavidin

Pierce™ Peroxidase Conjugate Stabilizer (Thermo Scientific™)

Thermo Scientific Pierce Peroxidase Conjugate Stabilizer is an antifreeze solution for storing freezer aliquots and stocks of antibodies and other HRP-conjugates in liquid form so they can be pipetted for use without thawing.

Features of Peroxidase Conjugate Stabilizer:

• Provides a buffered anti-freeze environment for enzyme conjugated antibodies and proteins
• Contains high-purity ethylene glycol, which maintains a liquid, low viscosity solution at -20°C
• Contains preservatives to prevent anti-microbial growth during long-term storage
• No aliquoting of conjugate is necessary and sampling is convenient

Pierce Peroxidase Conjugate Stabilizer is a storage solution for peroxidase-conjugated antibodies, streptavidin and other proteins (HRP conjugates). Mixing the Stabilizer in 2:1 or 1:1 ratio with HRP conjugate solutions (usually at least 1 mg/mL) produces a stabilized conjugate stock solution that remains a liquid at -20°C. This allows the conjugate to be pipetted directly from the sub-zero temperature condition, eliminating damaging freeze-thaw cycles. The highly purified solution is free of oxidizing agents and other contaminants that can harm enzyme activity, ensuring that stock solutions can be stored for months without significant loss of activity.

SuperBlock™ T20 (PBS) Blocking Buffer (Thermo Scientific™)

SuperBlock Blocking Buffer is optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer Dry Blend

eBioscience™ 20X TBS Wash Buffer for IHC/ICC (Invitrogen™)

The 20X TBS Wash Buffer for IHC/ICC is for use in immunohistochemistry and immunocytochemistry protocols which require washing to remove non-specific binding. This wash buffer is recommended for eFluor™ Nanocrystal conjugated-antibodies following antibody incubation. The TBS Wash Buffer is also compatible with organic dye-conjugated antibodies as well as unconjugated antibodies that may be used in two and three step staining protocols. The buffer is supplied as a 20X stock solution that should be diluted to 1X with distilled water.

Reported Application
Immunohistochemical Staining, Immunocytochemistry

SuperBlock™ (TBS) Blocking Buffer - Blotting (Thermo Scientific™)

Thermo Scientific SuperBlock (TBS) Blocking Buffer - Blotting is optimized TBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (TBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend

Enhancer Solution (Invitrogen™)

The InstantOne ELISA Enhancer Solution is a supplement added to the 5X Cell Lysis Buffer (Cat. No. IOCLB1) to yield the Cell Lysis Mix solution that is applied to cultured cells or tissues prior to running the assays. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with deionized water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.

Reported Application
ELISA

Coating Buffer B

The Invitrogen Coating Buffer B is intended for use with Invitrogen Antibody Pairs as part of the recommended assay procedure.

Guardian™ Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific™)

Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

Features of Guardian Peroxidase Conjugate Stabilizer/Diluent:

Preserves HRP activity—no significant loss of HRP activity over a six-month period at room temperature (1:1000 dilution) or 12 months at 4°C
Convenient—store ready-to-use dilutions (1:1000 to 1:100,000) that maintain enzyme activity in the refrigerator—no aliquoting or freezing
Assay compatible—simply add your favorite blocking buffer to create the ideal diluent for your HRP-based ELISA system or store the HRP conjugate as a 1:1000 stock solution for Western blots and dilute in the final assay buffer
Saves money—less expensive than competitor formulations or ordering new HRP conjugates

With Guardian Peroxidase Conjugate Stabilizer/Diluent, typical 1 mg/mL antibody or streptavidin peroxidase conjugates can be diluted as much as 100,000-fold for storage at 4°C. Most ELISA and blotting applications for HRP conjugates require dilution of typical 1 mg/mL stocks by at least 1000-fold (to 1 µg/mL). Sensitive assay systems, such as with chemiluminescent substrates, often require dilution up to 100,000-fold (10 ng/mL). The Guardian Solution enables these 1X working concentrations of HRP conjugate to be prepared in advanced and stored at 4°C for 12 months or at room temperature for six months.For blotting applications, it is usually best to prepare a concentration for storage that can be diluted 10-fold in assay buffer (which may contain blocking components) for final use.

Typical colorimetric ELISA procedures requires that 10 mL of HRP-conjugated secondary antibody be diluted 1000- to 5000-fold from a 1 mg/mL stock solution. That necessitates pipetting 2 to 10 µL of stock into a tube with 10 mL of diluent. More sensitive assay systems, such as Western blots with Thermo Scientific SuperSignal West Pico, Dura or Femto Chemiluminescent Substrates for HRP, require a second round of dilution to achieve the needed working concentration of HRP conjugate. With Guardian Stabilizer-Diluent, HRP conjugates can be pre-diluted and stored at concentrations that are either ready to use or easily diluted 10- to 100-fold for final use. Preparing larger volumes of pre-diluted stocks ensures consistency and greater accuracy for assays performed repeatedly day after day.

Pierce™ Diethanolamine Substrate Buffer (5X) (Thermo Scientific™)

Thermo Scientific Pierce Diethanolamine Substrate Buffer (5X) include optimized, reliable and convenient stock solutions of diethanolamine for alkaline phosphatase (alk-phos) detection.

Features of Diethanolamine Substrate Buffer (5X):

• Ready-to-use
• Minimize assay-to-assay variability

Diethanolamine Substrate Buffer is used with soluble alkaline phosphatase substrates such as PNPP. Our formulation is convenient, ready-to-use and reduces the possibility of assay-to-assay variability. Diethanolamine Substrate Buffer is sold as 5X concentrate with an optimal, consistent pH. It is stable even at a 1X concentration.

Related Products
Pierce™ Stable Peroxide Substrate Buffer (10X)

SEA BLOCK Blocking Buffer (Thermo Scientific™)

Thermo Scientific SEA BLOCK Blocking Buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.

Features of SEA BLOCK Blocking Buffer:

Non-mammalian—fish proteins are less likely to have specific binding interactions with antibodies and other mammalian proteins present in typical methods
Convenient—filtered and stabilized in PBS for compatibility with most assay systems
Easy to use—can be used as supplied or diluted up to 10-fold as needed
Flexible—may be used for many different applications, including as a diluent for antibodies

Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions while at the same time minimizing the possibility of cross-reaction with mammalian sample components. When normal sera cause background in immunohistochemistry (IHC), try this product. The product is also especially effective in various cellular imaging protocols based on visible and near-infrared fluorescence detection.

Fish serum has several advantages over typical blocking buffers. Because salmon is phylogenetically distant from mammals, which are the source of antibodies and samples used in most experiments, its serum proteins are less likely to have specific binding interactions with proteins used in typical cell biology experiments. Because SEA BLOCK Blocking Buffer is fish serum, it is effective in stabilizing solutions and samples for antibody-binding interactions. When normal sera cause background in IHC experiments, SEA BLOCK Blocking Buffer may correct the problem.

SEA BLOCK Blocking Buffer is particularly effective in applications involving fluorescence imaging. The blocker has been used to decrease background and increase signal-to-noise ratios with near-IR fluorescent probes and is validated for use with the LI-COR Odyssey™ Infrared Imaging System.

cAMP-Screen™ Assay/Lysis Buffer (Invitrogen™)

This Assay⁄Lysis Buffer is suitable for use with the cAMP-Screen® or cAmp-Screen Direct® Immunoassay System.

For Research Use Only. Not for use in diagnostics procedures.

Blocker™ BLOTTO in TBS (Thermo Scientific™)

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

Compare and view all available blocking buffers

Features of Blocker BLOTTO Blocking Buffer:
Format—ready-to-use 1X TBS solution; can be diluted as needed
Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen™)

The IHC Antigen Retrieval Solution- High pH (10X) is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. Dilution of this buffer to 1X using reagent grade water results in a 10 mM Tris, 1 mM EDTA solution, pH 9.0.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

eBioscience™ IHC /ICC Blocking Buffer - Low Protein (Invitrogen™)

The IHC/ICC Blocking Buffer- Low Protein is designed for use in immunohistochemistry and immunocytochemistry protocols which require blocking of non-specific binding sites. This blocking buffer is recommended for tissues and antibodies with average levels of background staining. This blocking buffer can be used both in the blocking step and as a diluent for unconjugated antibodies and organic dye-conjugated antibodies during the antibody incubation step. This buffer is supplied as a 1X stock solution containing sodium azide.

Reported Application
Immunohistochemical Staining, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections

InstantOne ELISA Cell Lysis Buffer (5X) (Invitrogen™)

Cell Lysis Buffer (5X) is included in the InstantOne ELISA kits. The solution is offered individually as some lab protocols require addition buffers.
Cell Lysis Buffer(5X) is used along with Enhancer Solution (Cat. No. IOES1) to make the Cell Lysis mixed used in the InstantOne phospho ELISA kits. The Cell Lysis Buffer (5X) contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Buffer (5X) is supplemented with Enhancer Solution to yield a versatile Cell Lysis Mix that can be applied to many cells and tissues. Please note the difference in names. Cell Lysis Mix is referred to heavily in the InstantOne phospho ELISA assay protocol.
A. The Cell Lysis Mix (5X) is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells.
B. The 1X Cell Lysis Mix is prepared by simply diluting Cell Lysis Mix (5X) (a mixture of the Cell Lysis Buffer (5X) and the Enhancer Solution) 5-fold with water. This buffer is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation. Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required.
NOTE: *Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays.

Reported Application
ELISA

StartingBlock™ T20 (PBS) Blocking Buffer (Thermo Scientific™)

StartingBlock Blocking Buffer is a single purified protein for fast blocking of western blots and ELISA assays. It provides broad compatibility with a wide range of antibodies, antibody combinations, and other protein probing and assay systems. StartingBlock buffer is available in PBS and TBS with and without Tween20.

Compare and view all available blocking buffers

Features of StartingBlock Blocking Buffers:
Strip and reprobe without reblocking—blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Cat. No. 21059)
Compatible with many detection systems—western blot, ELISA, and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times—less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Biotin- and serum protein-free formulation

Related products
StartingBlock (PBS) Blocking Buffer
StartingBlock (TBS) Blocking Buffer
StartingBlock T20 (TBS) Blocking Buffer

Pierce™ Protein-Free T20 (TBS) Blocking Buffer (Thermo Scientific™)

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a ready-to-use format.

Compare and view all available blocking buffers

Features of Protein-Free Blocking Buffer:
Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
Applications—western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

Related products
Pierce Protein-Free (PBS) Blocking Buffer
Pierce Protein-Free T20 (PBS) Blocking Buffer
Pierce Protein-Free (TBS) Blocking Buffer