Shop All Nuclease Control Reagents

RNase AWAY™ Surface Decontaminant (Thermo Scientific™)

Eliminate RNase and DNA from laboratory surfaces. RNase AWAY reduces dependency on carcinogenic DEPC treatments and saves time spent baking glassware.

RNaseZap™ RNase Decontamination Wipes Refill (Invitrogen™)

Ambion® RNaseZap Wipes are RNaseZap® in a convenient towel format. You simply wipe RNases off of surfaces and rinse with RNase-free water. Supplied as 3 rolls of 100 sheets plus solution to refill existing pop-up dispenser.
• Convenient towel format
• Completely removes RNase contamination from glass and plastic surfaces Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
• Works immediately on contact; no baking or autoclaving necessary The popular Ambion® RNaseZap® is now provided in a convenient wipe format. RNaseZap® Wipes are designed to eliminate RNase contamination on work surfaces, pipettors and equipment that must be RNase-free. RNaseZap® is a formulation of three ingredients known to be active against RNase. It effectively removes even high levels of RNase contamination that similar products cannot. With thorough rinsing, RNaseZap® leaves no residues that are inhibitory to enzymatic reactions. Note: The components are packaged separately. The wipes and RNaseZap® Solution are loaded into the pop-up dispenser prior to first use.

RNaseAlert™ Lab Test Kit (Invitrogen™)

Ambion® RNaseAlert® Lab Test kit is a Patent-pending technology detects RNase activity in a convenient and sensitive assay that delivers results in real time. Suitable for testing small sample numbers and can be used to ensure that solutions, tubes, tips, etc. are RNase-free; the kit contains sufficient reagents for 25 reactions.

• Detects as little as 3.5 x 10-7 units (~0.5 pg) of RNase A, with a fluorometer or by eye
• Simple, straightforward 30 min assay
• Monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected by eye upon illumination on a UV box, or with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution at 37°C for 30–60 min. The results are then read on a transilluminator or fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product:
The RNaseAlert® QC System is also available for high-throughput assays in a 96-well format (SKU# AM1966). RNaseAlert® is also fully compatible with the Ambion® DNaseAlert™ QC System (SKU# AM1970). Since the DNaseAlert™ Substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert®, the two kits can be used simultaneously with a fluorometer for quantitative real-time analyses of both RNases and DNases within a single sample.

DNA AWAY™ Surface Decontaminant (Thermo Scientific™)

Eliminate unwanted DNA and DNase from glassware and plasticware without affecting subsequent DNA samples. This surface decontaminant degrades DNA more quickly and effectively than autoclaving.

RNaseAlert™ QC System (Invitrogen™)

Ambion® RNaseAlert® QC System is a patent-pending technology detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Contain sufficient reagents for 5 × 96 high throughput assays.

• Detects as little as 3.5 x 10-7 units (~0.5 pg) of RNase A with a fluorometer
• Simple, straightforward 30 min assay
• Monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product:
RNaseAlert® is also fully compatible with the Ambion® DNaseAlert™ QC System (SKU# AM1970). Since the DNaseAlert™ Substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert®, the two kits can be used simultaneously with a fluorometer for quantitative real-time analyses of both RNases and DNases within a single sample.

RNaseAlert™ QC System v2 (Invitrogen™)

The RNaseAlert® QC System v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This version contains a substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 5 x 96 (480) high-throughput assays.

Features of RnaseAlert® QC System v2:

• Detects as little as ~0.3 pg of RNase A with a fluorometer
• Simple, straight-forward, 30-minute assay
• Designed to monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product
The RNaseAlert® QC System v2 is fully compatible with the DNaseAlert™ QC System. Since the DNaseAlert™ substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert® substrate, the two kits can be used simultaneously for quantitative real-time analyses of both RNases and DNases within a single sample.

ElectroZap™ Electrode Decontamination Solution (Invitrogen™)

Ambion® ElectroZap™ is specially formulated to instantaneously eliminate RNase contamination from electrodes. Supplied in one bottle containing 250 mL.

• Easily rinsed away leaving no residue
• Stable, safe, and non-corrosive
• No need for strong acid or alkali
• Also removes DNases and other proteins

Researchers performing RNA analysis often require the use of a pH meter to prepare their RNase-free solutions. The use of the same pH meter by several laboratory personnel, however, can often lead to problems with RNase contamination. This is especially a problem when preparing solutions that cannot be DEPC-treated or autoclaved. Special precautions must be taken when removing RNase contamination from a pH meter so that the accuracy will be unaffected and there is no residue left by the cleaning solution. Ambion® ElectroZap™ is a non-corrosive, stable cleaning solution that has been specially formulated to clean electrode surfaces in a simple, quick, and efficient manner. It is effective even against high levels of RNase or DNase contamination. ElectroZap® is simply sprayed directly onto the electrode and then rinsed off with RNase-free water. It does not leave behind any residue that may interfere with subsequent enzymatic reactions.

RNAsecure™ Resuspension Solution (1 ml Tube) (Invitrogen™)

Ambion® RNAsecure Resuspension Solution (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. It is supplied at working concentration for direct resuspension of RNA pellets. Ten tubes containing 1 mL each are provided.

• No post-treatment autoclaving
• Safe, easy-to-use reagent inactivates RNases in solution
• Inactivation process can be repeated to protect against newly introduced contaminants
• Compatible with downstream procedures, such as RT-PCR and in vitro transcription

RNAsecure™ Resuspension Solution is specifically meant for resuspending RNA pellets that may contain trace amounts of ribonuclease (e.g., after total RNA isolation). Note: it cannot reverse RNA degradation that has already occurred, but can prevent further RNA degradation. To use, the RNA pellet is resuspended in the RNAsecure Resuspension Solution and heated to 60°C for 10 min. It can be reheated to eliminate any RNase contamination introduced at a later time.

RNase AWAY™ Decontamination Reagent (Invitrogen™)

RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive.

Performance and quality testing
RNase AWAY® Reagent is functionally tested for the elimination of RNase activity. No detectable RNase activity is observed.

DNaseAlert™ QC System (Invitrogen™)

Ambion® DNaseAlert™ QC System is a patent pending technology provides the sensitive detection of DNases in a simple, easy-to-use fluorimetric assay that delivers results in real time. Contains sufficient reagents for 5 × 96 high throughput assays.

• Detect a range of different DNases in minutes
• Certify plastics, enzymes, solutions, and other biomaterials as "DNase-free" prior to DNase-sensitive applications such as PCR
• Combine with RNaseAlert® to quantitatively resolve both RNase and DNase activity simultaneously

The DNaseAlert™ QC System contains a unique DNA substrate tagged with both a fluorescent reporter and a dark quencher. The uncleaved substrate exhibits minimal fluorescence due to the proximity of the fluor and the quencher. When DNases are present, the linkage between the fluor and quencher is severed, which leads to the emission of a bright orange signal upon excitation at the proper wavelength of light. The extent of cleavage of the substrate is proportional to the level of DNase contamination and can be monitored as an increase in fluorescence on a fluorometer.

Fast and Simple Protocol
The DNaseAlert™ QC System protocol is fast and simple. The substrate is reconstituted with TE buffer and then aliquoted into a 96-well plate containing the reaction buffer. Test samples are then added and fluorescence is monitored to determine the results of the assay. Enough substrate is provided for 500 reactions. The DNaseAlert™ QC System also includes both negative and positive controls for accurately identifying contaminated samples. DNaseAlert™ is a kinetically reliable assay. Data can be collected in real time, and the initial velocities are dose-dependent on the amount of input DNase I.

Accessory Product:
DNaseAlert™ was designed to interface seamlessly with the RNaseAlert® QC System (SKU# AM1966) and permit two-color detection of both DNases and RNases in the same sample. The fluorescent reporter linked to the DNaseAlert™ substrate is spectrally distinct from the fluorescent tag on the RNaseAlert® substrate.

RNaseAlert™ Lab Test Kit v2 (Invitrogen™)

RNaseAlert® Lab Test Kit v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This kit is suitable for testing small numbers of sample and ensuring that solutions, tubes, tips, etc. in your lab are RNase-free. This new version contains a more sensitive substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 25 reactions.

• Detects as little as ~0.3 pg of RNase A with a fluorometer
• Simple, straight-forward 30-minute assay

The RNaseAlert® Lab Test Kit v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

RNaseZap™ RNase Decontamination Solution (Invitrogen™)

RNaseZap® RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap® Solution onto the surface to be decontaminated and rinse it off with RNase-free water. Features of RNaseZap® RNase Decontamination Solution:

• Completely removes RNase contamination from glass and plastic surfaces
• Excels at removing high levels of RNase contamination whereas similar products fail
• Proven effective at removing high concentrations of dried-on RNase A
• Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free

Using RNaseZap® Solution
Working with RNA requires that special measures be taken to ensure an RNase-free environment. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results in RPAs and Northerns. RNaseZap® contains three ingredients active against RNase and has proven to be extremely effective at removing RNase contamination from glassware, plastic surfaces, countertops, and pipettors. It has also been shown to be effective at eliminating RNase contamination from microfuge tubes without inhibiting subsequent enzymatic reactions. Supplied in one 250 mL bottle.

RNase contamination in microfuge tubes
While concern about RNase contamination of frequently handled labware is common and prudent, researchers often do not worry about RNase contamination in commercially prepared products such as microfuge tubes. However, in a survey of six standard 1.5 mL microfuge tubes in which three were pre-rinsed with RNaseZap® and water and three were left untreated, all of the untreated tubes had at least marginal levels of RNase contamination, whereas none of the tubes pre-rinsed with RNaseZap® exhibited contamination. Additionally, in testing commercially available "RNase-free" microfuge tubes, approximately 10% had some RNase contamination (data not shown). This may well account for the erratic results that researchers periodically experience when performing RNA-based assays.

RNAsecure™ RNase Inactivation Reagent (Invitrogen™)

Ambion® RNAsecure (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 10 mL supplied.
• No post-treatment autoclaving
• Safe, easy-to-use reagent inactivates RNases in solutions
• Inactivation process can be repeated to protect against newly introduced contaminants
• Compatible with downstream procedures, such as RT-PCR and in vitro transcription
• Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and, at worst, can cause experimental failure. Traditionally, DEPC has been used to treat solutions that come into contact with RNA. DEPC treatment is time-consuming, possibly hazardous, and only eliminates RNases present at the time of DEPC treatment. In addition, some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. Finally, DEPC has to be inactivated by autoclaving post-treatment to prevent it from interfering with downstream enzymatic reactions. RNAsecure Reagent eliminates these problems. Unlike DEPC, RNAsecure can be used on virtually any solution and does not require post-treatment autoclaving. A unique feature of RNAsecure is that reheating after the initial treatment will reactivate the RNase-destroying agent to eliminate any new contaminants. RNAsecure is supplied as a 25X concentrated stock. It is added to solutions as part of reactions (in vitro transcription, RT-PCR, etc.), prior to the addition of enzymes, and heated to 60°C for 10 min to inactivate RNase.

DNAZap™ PCR DNA Degradation Solutions (Invitrogen™)

DNAZap™ Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. Features of DNAZap™ Solutions:

• Completely degrades contaminating DNA and RNA at the level of PCR sensitivity
• Works on contact
• Degrades nucleic acid, unlike other products on the market that only act as detergents
• Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tubes

Using DNAZap™ Solutions
PCR and related amplification techniques produce large amounts of a target nucleic acid sequence. This exquisite sensitivity creates its own drawbacks, primarily the potential amplification of undesirable, contaminating nucleic acids along with the desired sequence—and sometimes at the expense of the desired sequence. DNAZap Solutions provide a convenient method to decontaminate a variety of surfaces in a matter of minutes. It even effectively eliminates contaminating DNA in PCR tubes without inhibiting subsequent enzymatic reactions. All contaminating nucleic acid is degraded to nucleotides, preventing any chance of false positive amplification.