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TrueGuide™ tracrRNA (Invitrogen™)

Invitrogen TrueGuide Synthetic tracrRNA is a 72-bp gRNA scaffold that hybridizes with TrueGuide Synthetic crRNAs to form a gRNA complex that directs Cas9 nuclease to a specific editing site within the genome. The performance of this two-piece gRNA system is boosted by the addition of 2’O-methyl analogs and 3’ phosphorothioate internucleotide linkages. These modifications enhance editing efficiency by increasing binding to the target site and inhibiting nuclease degradation, respectively. The modifications are incorporated into the terminal three nucleotides on the 5’ and 3’ ends of both the TrueGuide Synthetic tracrRNA and the TrueGuide Synthetic crRNAs. TrueGuide Synthetic tracrRNA is available in three package sizes: 5 nmol, 20 nmol, and 100 nmol.

TrueGuide™ sgRNA Positive Control, CDK4 (human) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against CDK4 targets a region of Exon 2 and has achieved editing efficiencies of up to 80% in a variety of human cell lines. Use the CDK4 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ sgRNA Negative Control, non-targeting 1 (Invitrogen™)

TrueGuide Negative Controls are non-targeting gRNA sequences that do not recognize any sequence in the human or mouse genome and provide a non-edited control when performing analysis on pooled populations of edited cells.

TrueGuide™ sgRNA Positive Control, HPRT1 (human) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against HPRT1 targets an intronic region and has achieved editing efficiencies of up to 65% in a variety of human cell lines. Use the HPRT1 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ crRNA Positive Control, CDK4 (human) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against CDK4 targets a region of Exon 2 and has achieved editing efficiencies of up to 80% in a variety of human cell lines. Use the CDK4 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ crRNA Positive Control, Rosa26 (mouse) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against ROSA26 targets an intronic region of the gene and has achieved editing efficiencies of up to 80% in a variety of mouse cell lines. Use the ROSA26 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ sgRNA Positive Control, AAVS1 (human) (Invitrogen™)

Invitrogen TrueGuide Synthetic gRNAs are ready-to-use, synthetic gRNAs enabling researchers to truly harness the power of genome editing. TrueGuide Synthetic gRNAs both maximize and simplify the performance of genome editing experiments so researchers can create more relevant cellular models and make new discoveries.

GeneChip™ Poly-A RNA Control Kit (Applied Biosystems™)

The GeneChip™ Poly-A RNA Control Kit is used with the Prokaryotic Target Labeling Assay and the Prokaryotic Target Labeling Assay for 3' expression studies with prokaryotic GeneChip Brand Arrays. The assays utilize reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated GeneChip DNA Labeling Reagent at the 3' termini.

Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.

Prokaryotic Target Labeling Assay components include:
B2 Control Oligo (3nM)
GeneChip DNA Labeling Reagent
GeneChip Poly-A RNA Control Kit

Related Links
GeneChip™ Wash Buffer A
GeneChip™ Wash Buffer B

TrueGuide™ sgRNA Positive Control, Rosa26 (mouse) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against ROSA26 targets an intronic region of the gene and has achieved editing efficiencies of up to 80% in a variety of mouse cell lines. Use the ROSA26 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ crRNA Positive Control, AAVS1 (Human) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize editing performance. This positive control targeting AAVS1 targets an intronic region of the AAVS1 locus and has achieved editing efficiencies of up to 60% in a variety of human cell lines. Use the AAVS1 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence, and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ crRNA Positive Control, HPRT1 (human) (Invitrogen™)

Thermo Fisher Scientific provides a complete collection of validated positive and negative controls for the TrueGuide Synthetic gRNA product line to help maximize your editing performance. This positive control against HPRT1 targets an intronic region and has achieved editing efficiencies of up to 65% in a variety of human cell lines. Use the HPRT1 control to easily determine the optimal conditions for delivering CRISPR/Cas9 tools to your cell type, allowing you to move forward in your editing experiments with confidence and minimize downstream effort to isolate and validate your edited clones.

TrueGuide™ crRNA Negative Control, non-targeting 1 (Invitrogen™)

Invitrogen TrueGuide Synthetic gRNAs are ready-to-use, synthetic gRNAs enabling researchers to truly harness the power of genome editing. TrueGuide Synthetic gRNAs both maximize and simplify the performance of genome editing experiments so researchers can create more relevant cellular models and make new discoveries.