Shop All Oligo Synthesis Reagents

Ac-rC Phosphoramidite, TheraPure™ grade, 20-400 finish bottle (Thermo Scientific™)

Ac-rC CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 30 mL Amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-[(tert-butyl)dimethylsilyl]-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C47H64N5O9PSi
• Base—rC
• Base—rC
• Base Protection—Ac
• Formula weight—902.1 g/mol
• CAS number—121058-88-6
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

Beta Cyanoethyl Phosphoramidite, T (Applied Biosystems™)

The T Beta Cyanoethyl Phosphoramidite is used as a component of a deprotecting chemistry set including dA(Bz), dC(Bz) and dG(iBu). Standard series of phosphoramidite deprotects in 6-8 hours at 55°C.

β-cyanoethyl phosphoramidites are for use on ABI synthesizers to guarantee you high quality nucleic acid synthesis.

• Proprietary manufacturing processes yield high-purity β-cyanoethyl phosphoramidites, which in turn guarantee production of high-quality DNA at all scales.
• β-cyanoethyl phosphoramidites are use-tested and optimized for use on the ABI synthesizers to guarantee you high-quality nucleic acid synthesis.

High Quality
Our phosphoramidites benefit from proprietary manufacturing processes that yield high-purity products. In synthesis they perform at a correspondingly high level, producing pure, high quality DNA at all scales. We guarantee that 5'-dimethoxytrityl nucleoside β-cyanoethyl phosphoramidites, monomer intermediates for licensed β-cyanoethyl phosphoramidite chemistry, will produce DNA that is at least 99.8% chemically authentic when used with recommended synthesis cycles.

Dry phosphoroamidites (powder form) are guaranteed for one year from the date of shipment. Lifetime of diluted phosphoramidites in residence on the instrument is two weeks when used with CPG synthesis supports, and three weeks when used with polystyrene supports.

For Research Use Only. Not for use in diagnostics procedures.

Ac-rC Phosphoramidite, TheraPure™ grade, 28-400 finish bottle (Thermo Scientific™)

Ac-rC CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 30 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-[(tert-butyl)dimethylsilyl]-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C47H64N5O9PSi
• Base—rC
• Base Protection—Ac
• Formula weight—902.1 g/mol
• CAS number—121058-88-6
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

Beta Cyanoethyl Fastphoramidite™, dG(dmf) (Applied Biosystems™)

The dG(dmf) Beta Cyanoethyl Fastphoramidite® is used as a component of a fast deprotecting chemistry set including dA(Bz), dC(Bz) and T. FastPhoramidite® series deprotects in 1 hour at 65°C.

β-cyanoethyl phosphoramidites are for use on ABI synthesizers to guarantee you high quality nucleic acid synthesis.
• Proprietary manufacturing processes yield high-purity β-cyanoethyl phosphoramidites, which in turn guarantee production of high-quality DNA at all scales.
• FastPhoramidite® reagents deprotect in one hour at 65°C to shorten overall oligonucleotide production times and costs.
• β-cyanoethyl phosphoramidites are use-tested and optimized for use on the ABI synthesizers to guarantee you high-quality nucleic acid synthesis.

High Quality
Our phosphoramidites benefit from proprietary manufacturing processes that yield high-purity products. In synthesis they perform at a correspondingly high level, producing pure, high quality DNA at all scales. We guarantee that 5'-dimethoxytrityl nucleoside β-cyanoethyl phosphoramidites, monomer intermediates for licensed β-cyanoethyl phosphoramidite chemistry, will produce DNA that is at least 99.8% chemically authentic when used with recommended synthesis cycles.

Fast Results
FastPhoramidite® reagents offer fast deprotection for primer-length oligonucleotides. Simply substituting Fast G, dG(dmf), for our standard dG(iBu) shortens deprotection at 55°C from eight hours to two. Elevating the temperature to 65°C further cuts deprotection to 1 hour.

Dry phosphoroamidites (powder form) are guaranteed for one year from the date of shipment. Lifetime of diluted phosphoramidites in residence on the instrument is two weeks when used with CPG synthesis supports, and three weeks when used with polystyrene supports.

For Research Use Only. Not for use in diagnostics procedures.

2'-OMe-Ac-C Phosphoramidite, standard grade, 20-400 finish bottle (Thermo Scientific™)

2'-OMe-Ac-C CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with 2'-O-methyl protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methyl (OMe) oligo modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The "standard grade" marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 30-mL amber bottle with a 20-400 finish neck style, which is compatible with most Expedite synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-methyl-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C42H52N5O9P
• Base—C
• Base Protection—Ac
• Formula weight—801.86 g/mol
• CAS number—199593-09-4
• HPLC purity—≥98.0%; Sum of PIII impurities (140-152ppm) ≤0.5%
31P NMR purity—≥98.0%

T Phosphoramidite, TheraPure™ grade, serum vial bottle (Thermo Scientific™)

T CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxythymidine (dT) for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups (except T and U) designed specifically to protect the DNA during the chemical synthesis process, and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. The TheraPure trademark denotes a highest level of phosphoramidite purity, developed with the highest stringency of of high definiteion analytics and tightly controlled impurity profiles. Ideal for any therapeutic application. This product is supplied in a 60 mL Amber bottle with a Serum Vial neck style, which is compatible with most ABI Systems|AKTA Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C40H49N4O8P
• Base—T
• Formula weight—744.82 g/mol
• CAS number—98796-51-1
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; Single critical impurity ≤0.3%; Presence of dC, dG, or T impurity ≤0.03%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%; No peak(s) above 170 ppm; HMT impurity (~146ppm): ≤0.10%

2'-MOE-5mC Phosphoramidite, TheraPure™ grade, 28-400 finish bottle (Thermo Scientific™)

2'-MOE-5mC CEP is a phosphoramidite containing 5' DMT and the nucleoside 5-methyl-cytidine (5mC) with 2'-O-methoxy-ethyl (MOE) protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methoxy-ethyl (MOE) modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 30-mL amber bottle with a 28-400 finish neck style, which is compatible with most MerMade synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C50H60N5O10P
• Base—5mC
• Base Protection—Bz
• Formula weight—922.0 g/mol
• CAS number—163759-94-2
• HPLC purity—≥99%; M-11 Impurity ≤0.01%
31P NMR purity—≥99%

CE Phosphoramidite, dA(Bz) (Applied Biosystems™)

The dA(Bz) CE Phosphoramidite is used for nucleic acid synthesis on ABI DNA Synthesizers. Standard series of phosphoramidite deprotects in 6-8 hours at 55°C.

• Proprietary manufacturing processes yield high-purity β-cyanoethyl phosphoramidites, which in turn guarantee production of high-quality DNA at all scales.
• β-cyanoethyl phosphoramidites are use-tested and optimized for use on the ABI synthesizers to guarantee you high-quality nucleic acid synthesis.

High Quality
Our phosphoramidites benefit from proprietary manufacturing processes that yield high-purity products. In synthesis they perform at a correspondingly high level, producing pure, high quality DNA at all scales. We guarantee that 5'-dimethoxytrityl nucleoside β-cyanoethyl phosphoramidites, monomer intermediates for licensed β-cyanoethyl phosphoramidite chemistry, will produce DNA that is at least 99.8% chemically authentic when used with recommended synthesis cycles.

Dry phosphoroamidites (powder form) are guaranteed for one year from the date of shipment. Lifetime of diluted phosphoramidites in residence on the instrument is two weeks when used with CPG synthesis supports, and three weeks when used with polystyrene supports.

For Research Use Only. Not for use in diagnostics procedures.

Ac-rC Phosphoramidite, standard grade, serum vial bottle (Thermo Scientific™)

Ac-rC CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The "Standard Grade" marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 60 mL Amber bottle with a Serum Vial neck style, which is compatible with most ABI Systems|AKTA Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-TBDMSi-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C47H64N5O9PSi
• Base—rC
• Base Protection—Ac
• Formula weight—902.1 g/mol
• CAS number—121058-88-6
• HPLC Purity—≥98.0%
• 31P NMR Purity—≥98.0%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%

Ac-rC Phosphoramidite, TheraPure™ grade, wide mouth bottle (Thermo Scientific™)

Ac-rC CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. RNA phosphoramidites contain a 2'-OH protecting group, tBDMS (tert-butyl dimethylsilyl). Each base has unique protecting groups designed specifically to protect the RNA during the chemical synthesis process, and produce the highest amount of full length product. Alternate base protection options are also available to allow for faster deprotection and longer oligonucleotide synthesis. The TheraPure trademark denotes our highest level of phosphoramidite purity, developed with the greatest stringency of high definition analytics and tightly controlled impurity profiles and, therefore, ideal for any therapeutic application. This product is supplied in a 250 mL Opaque bottle with a Wide Mouth neck style, which is compatible with most None (Bulk) of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-[(tert-butyl)dimethylsilyl]-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C47H64N5O9PSi
• Base—rC
• Base Protection—Ac
• Formula weight—902.1 g/mol
• CAS number—121058-88-6
• HPLC Purity—≥99%; M-11 Impurity: ≤0.01%
• 31P NMR Purity—≥99%; Sum of non-primary peaks in 140-152 ppm range: ≤0.5%; No single PIII impurity >0.3%

Ac-dC Phosphoramidite, TheraPure™ grade, 28-400 finish bottle (Thermo Scientific™)

Ac-dC CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxycytidine (dC) with Acetyl (Ac) base protection, for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups designed specifically to protect the DNA during the chemical synthesis process and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. By using Ac-dC, Bz-dA, and DMF-dG monomers, fast cleavage and deprotection can be effected by a 1:1 mixture of aqueous ammonium hydroxide and aqueous methylamine (or AMA) in 10 min. This product is supplied in a 250 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-Acetyl-2'-deoxycytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
• Molecular formula—C41H50N5O8P
• Base—dC
• Base Protection—Ac
• Formula weight—771.8 g/mol
• CAS number—154110-40-4
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; No single impurity >0.5%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%

T Phosphoramidite, standard grade, 20-400 finish bottle (Thermo Scientific™)

T CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxythymidine (dT) for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups (except T and U) designed specifically to protect the DNA during the chemical synthesis process, and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. The "Standard Grade" marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 30 mL Amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C40H49N4O8P
• Base—T
• Formula weight—744.82 g/mol
• CAS number—98796-51-1
• HPLC Purity—≥98.0%
• 31P NMR Purity—≥98.0%

Tetrazole Acetonitrile Solution (Applied Biosystems™)

Each contains 450 mL Tetrazole Acetonitrile Solution.

For Research Use Only. Not for use in diagnostics procedures.

2'-OMe-Ac-C Phosphoramidite, standard grade, 28-400 finish bottle (Thermo Scientific™)

2'-OMe-Ac-C CEP is a phosphoramidite containing 5' DMT and the nucleoside cytidine (C) with 2'-O-methyl protection, for use in oligonucleotide synthesis. Located in the ideal oligo position, 2'-O-methyl (OMe) oligo modifications display nuclease resistance, fewer off-target effects, and slightly increased hybridization affinities. These phosphoramidites are commonly used for oligos designed for antisense, siRNA, aptamer, or gRNA-based research. The "standard grade" marking denotes a baseline level of purity verified with analytical methods, and proven to be suitable for most oligo synthesis requirements. This product is supplied in a 30-mL amber bottle with a 28-400 finish neck style, which is compatible with most MerMade synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-2'-O-methyl-cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C42H52N5O9P
• Base—C
• Base Protection—Ac
• Formula weight—801.86 g/mol
• CAS number—199593-09-4
• HPLC purity—≥98.0%; Sum of PIII impurities (140-152ppm) ≤0.5%
31P NMR purity—≥98.0%

T Phosphoramidite, TheraPure™ grade, 28-400 finish bottle (Thermo Scientific™)

T CEP is a phosphoramidite containing 5' DMT and the nucleoside deoxythymidine (dT) for use in oligonucleotide synthesis. DNA phosphoramidites are the building blocks for synthesis of DNA or DNA containing oligonucleotides. Each base has unique protecting groups (except T and U) designed specifically to protect the DNA during the chemical synthesis process, and produce the highest amount of full length product. Alternative protecting groups on dC (acetyl) and dG (DMF) allow for a faster deprotection of the oligonucleotide. The TheraPure trademark denotes a highest level of phosphoramidite purity, developed with the highest stringency of of high definiteion analytics and tightly controlled impurity profiles. Ideal for any therapeutic application. This product is supplied in a 250 mL Amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers.

Features:
• Full chemical name—5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C40H49N4O8P
• Base—T
• Formula weight—744.82 g/mol
• CAS number—98796-51-1
• HPLC Purity—≥99%; M-11 Impurity ≤0.01%; Single critical impurity ≤0.3%; Presence of dC, dG, or T impurity ≤0.03%
• 31P NMR Purity—≥99%; Sum of non-primary peaks at 140-152 ppm: ≤0.3%; No peak(s) above 170 ppm; HMT impurity (~146ppm): ≤0.10%