Shop All DNA⁄RNA Purification Replacement Buffers

MagMAX™ Wash Solution 1 Concentrate (Invitrogen™)

Ambion® MagMAX™ Wash Solution 1 Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 205 mL bottle.

Lysis Buffer for MagJET RNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET RNA Kit (K2731/2732) and may be purchased separately.

Wash Buffer 1 for MagJET Blood RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately. Provided in 80 mL aliquots.

Wash Buffer 1 for MagJET RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of several MagJET nucleic acid purification kits and may be purchased separately. Provided in 80 mL aliquots. Please consult kit protocols to confirm catalog numbers for re-ordering the appropriate kit-specific components.

PureLink™ PCR Purification Wash Buffer W1 (Invitrogen™)

PureLink™ PCR Purification Wash Buffer B1 is the wash buffer used with the PureLink™ PCR Purification Kit. The buffer is activated by the addition of 160 mls of 100% ethanol.

Lysis Buffer for MagJET Blood gDNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Whole Blood gDNA Kits (K2741/2742) and may be purchased separately.

Wash Buffer 2 for MagJET Blood RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 2 (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately. Provided in 30 mL aliquots.

Wash Buffer for GeneJET Gel Extraction Kit (Thermo Scientific™)

Thermo Scientific GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit (K0691/K0692) and may be purchased separately. Provided in 45 mL aliquots.

Binding Buffer for MagJET Blood gDNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Binding Buffer (conc.) is a component of the MagJET Whole Blood gDNA Kits (K2741, K2742) and may be purchased separately.

Wash Buffer 1 for MagJET Plant RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Plant RNA DNA Kits (K2771/2772) and may be purchased separately. Provided in 110 mL aliquots.

Wash Solution II (concentrated) (Thermo Scientific™)

Thermo Scientific GeneJET Wash Solution II (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 180 mL aliquots.

PureLink™ Genomic Lysis/Binding Buffer (Invitrogen™)

The PureLink™ Genomic DNA Mini Kit and PureLink™ 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink™ Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink™ Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink™ 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink™ Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink™ 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink™ gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink™ Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink™ Genomic DNA Mini Kit includes PureLink™ Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink™ 96 Genomic DNA Kit includes PureLink™ Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

Chargeswitch™ Pro ET Removal Wash Buffer (Invitrogen™)

Chargeswitch® Pro ET Removal Wash Buffer is a component in the Chargeswitch® Pro Filter Plasmid kits. With the endotoxin removal buffer, the endotoxin levels of <10 EU⁄ug can be achievable and it can be used in all Chargeswitch® Pro plasmids.

Resuspension Solution for GeneJET Plasmid Miniprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Resuspension Solution is a component of the GeneJET Plasmid Miniprep Kit (K0502/K0503) and may be purchased separately.

Highlights

• The Resuspension Solution should be stored at 4°C and is stable for 6 months.
• After the addition of RNase A, the Resuspension Solution is stable for 6 months when stored at 4°C.

Related Products
Neutralization Solution for GeneJET Plasmid Miniprep Kit
Wash Solution for GeneJET Plasmid Miniprep Kit
Binding Buffer for GeneJET Plasmid Miniprep Kit
GeneJET NGS Cleanup Kit
Binding Buffer for GeneJET NGS Cleanup Kit
Prewash Buffer for GeneJET NGS Cleanup Kit
Wash Buffer for GeneJET NGS Cleanup Kit
GeneJET Plasmid Miniprep Kit
RNase A
Elution Buffer for GeneJET Plasmid Miniprep Kit
Lysis Solution for GeneJET Plasmid Miniprep Kit