Shop All DNA⁄RNA Purification Replacement Buffers

Neutralization Solution (Thermo Scientific™)

Thermo Scientific GeneJET Neutralization Solution is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 240 mL aliquots.

PrepSEQ™ Lysis Buffer (Applied Biosystems™)

PrepSEQ™ Lysis Buffer is a component of the PrepSEQ™ Express Nucleic Acid Extraction Kit (Cat. No. 4466351), offered separately here for those kit users who do large-quantity nucleic acid extractions and would benefit from additional lysis buffer. As with other products in the Pharma Analytics Sample Preparation product line, PrepSEQ Lysis Buffer:

• Is completely supported by our global manufacturing and distribution centers
• Has a Certificate of Analysis available
• Has a shelf-life of 12 months

PureLink™ Viral Lysis Buffer (Invitrogen™)

The PureLink™ 96 Viral RNA/DNA Kit is a 96-well silica plate system that can extract both viral RNA and DNA from 200-µl cell-free fluids which uses a low-depth 96-well centrifuge bucket for ease-of- use, all without any loss in sensitivity. These kits provide flexibility in centrifuge choice. Minimum speeds for 96 well processing of 2250 x g and a rotor bucket depth of 5 cm make this kit one of the most adaptable systems for existing laboratory centrifuges

Rebinding Buffer for MagJET Plant RNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Rebinding Buffer is a component of the MagJET Plant RNA DNA Kit (K2771) and may be purchased separately.

Endotoxin Binding Reagent (Thermo Scientific™)

Thermo Scientific GeneJET Endotoxin Binding Reagent is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 60 mL aliquots.

Wash Solution II, RNA Purification (Invitrogen™)

Applied Biosystems® replacement solution for use with TransPrep Chemistry is for the isolation on the ABI PRISM® 6100 Nucleic Acid PrepSation.

Rebinding Buffer for MagJET Blood RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Rebinding Buffer (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately.

Wash Buffer 1 for MagJET Blood RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately. Provided in 80 mL aliquots.

MagMAX™ Lysis/Binding Solution Concentrate (Invitrogen™)

Ambion® MagMAX™ Lysis⁄Binding Solution Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 100 mL bottle.

This solution is the same formulation as the lysis buffer used in Cat. Nos. AM1830, AM1835, AM1836, AM1837, AM1839, AM1840, 4462359, and A27828.

PureLink™ Genomic Digestion Buffer (Invitrogen™)

The PureLink™ Genomic DNA Mini Kit and PureLink™ 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink™ Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink™ Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink™ 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink™ Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink™ 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink™ gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink™ Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink™ Genomic DNA Mini Kit includes PureLink™ Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink™ 96 Genomic DNA Kit includes PureLink™ Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

Precipitation Solution for MagJET Plant gDNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Precipitation Solution is a component of the MagJET Plant Genomic DNA Kits (K2761/2762) and may be purchased separately. Provided in 16.5 mL aliquots.

Resuspension Solution for MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Resuspension Solution is a component of the MagJET Plasmid DNA Kit (K2791) and may be purchased separately.

SequalPrep™ Wash Buffer (Applied Biosystems™)

For use with SequalPrep™ Normalization Plates, the wash buffer may be purchased separately.

Elution Buffer for GeneJET Plasmid Midiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Elution Buffer is a component of the GeneJET Plasmid Midiprep Kit and may be purchased separately. Provided in 60 mL aliquots.

Wash Buffer 1 for MagJET Plasmid DNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately. Provided in 110 mL aliquots.

PureLink™ Genomic Wash Buffer 2 (Invitrogen™)

The PureLink® Genomic DNA Mini Kit and PureLink® 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink® Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink® Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink® 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink® Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink® 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink® gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink® Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink® Genomic DNA Mini Kit includes PureLink® Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink® 96 Genomic DNA Kit includes PureLink® Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

Wash Solution I, RNA Purification (Invitrogen™)

This Wash Solution is for use with the Total RNA Chemistry System.

Total RNA Chemistry makes possible the rapid isolation of total RNA from various samples, including tissue-cultured cells, whole blood, and peripheral blood mononuclear cells (PBMCs). The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

For Research Use Only. Not for use in diagnostics procedures.

Wash Buffer 1 for MagJET Plant RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Plant RNA DNA Kits (K2771/2772) and may be purchased separately. Provided in 110 mL aliquots.

MagMAX™ Wash Solution 1 Concentrate (Invitrogen™)

Ambion® MagMAX™ Wash Solution 1 Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 205 mL bottle.

Neutralization Solution for MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Neutralization Solution is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately. Provided in 25 mL aliquots.

PureLink™ Genomic Lysis/Binding Buffer (Invitrogen™)

The PureLink™ Genomic DNA Mini Kit and PureLink™ 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink™ Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink™ Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink™ 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink™ Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink™ 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink™ gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink™ Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink™ Genomic DNA Mini Kit includes PureLink™ Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink™ 96 Genomic DNA Kit includes PureLink™ Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

Lysis Buffer for MagJET Plant RNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Plant RNA DNA Kits (K2771/2772) and may be purchased separately.

Lysis Buffer for MagJET Viral Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Viral DNA and RNA Kits (K2781/2782) and may be purchased separately.

PureLink™ 384 Pre-Lysis Buffer (Invitrogen™)

The PureLink™ 384 Plasmid Purification Plate is designed for ultra high-throughput DNA isolation. By utilizing the same technology as the PureLink™ 96 Plasmid Purification System, the PureLink™ 384 Plasmid Purification Plate reduces time and effort while reliably isolating high quality plasmid DNA for DNA sequencing, PCR, and restriction digests.

• PureLink™ 96: Sample size 1.3 ml culture; Typically yields up to 3-5 µg
• PureLink™ 384: Sample size 200 µl culture; Typically yields up to 2-3 µg
• Purify 96 or 384 plasmid DNA samples simultaneously
• Unique centrifugation method eliminates cross-contamination

The PureLink™ 96 Plasmid Purification System is designed for high-throughput isolation of high copy number plasmids. Following the PureLink™ 96 Plasmid Purification Systems protocol, up to 96 plasmid DNA samples can be simultaneously purified. The novel technology eliminates time-consuming cell harvesting and alkaline lysis steps (Figure 1), saving time and effort. The unique centrifugation-based format of the PureLink™ 96 Plasmid Purification System eliminates problematic cross-contamination between wells, a critical problem in PCR and microarray analysis. The isolated plasmid DNA is ideal for use in automated sequencing reactions, offering superior read lengths and data quality. Samples may be processed 96 at a time or in smaller increments. The PureLink™ 96 Plasmid Purification System is compatible with a variety of centrifuges, robots, pipetting devices, automated sequencers, and sequencing chemistries.

Wash Solution I (concentrated) (Thermo Scientific™)

Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 360 mL aliquots.

Lysis Buffer for MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately.

Wash Buffer for GeneJET Gel Extraction Kit (Thermo Scientific™)

Thermo Scientific GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit (K0691/K0692) and may be purchased separately. Provided in 45 mL aliquots.

Chargeswitch™ Pro ET Removal Wash Buffer (Invitrogen™)

Chargeswitch® Pro ET Removal Wash Buffer is a component in the Chargeswitch® Pro Filter Plasmid kits. With the endotoxin removal buffer, the endotoxin levels of <10 EU⁄ug can be achievable and it can be used in all Chargeswitch® Pro plasmids.

Elution Solution, Nucleic Acid Purification (Invitrogen™)

The Elution Solution is used in the purification of total RNA from cells, primary cell isolates, animal and plant tissues and tumors.

Use With:
Total RNA Lysis Solution, Nucleic Acid Purification.

For Research Use Only. Not for use in diagnostics procedures.