Shop All DNA⁄RNA Purification Replacement Buffers

Lysis Solution (Thermo Scientific™)

Thermo Scientific GeneJET Lysis Solution is a component of several GeneJET nucleic acid purification kits and may be purchased separately. Please consult kit protocols to confirm catalog numbers for re-ordering the appropriate kit-specific components.

Lysis Buffer for MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately.

PureLink™ Genomic Lysis/Binding Buffer (Invitrogen™)

The PureLink™ Genomic DNA Mini Kit and PureLink™ 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink™ Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink™ Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink™ 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink™ Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink™ 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink™ gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink™ Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink™ Genomic DNA Mini Kit includes PureLink™ Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink™ 96 Genomic DNA Kit includes PureLink™ Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

Neutralization Solution for MagJET Plasmid DNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Neutralization Solution is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately. Provided in 25 mL aliquots.

Precipitation Solution for MagJET Plant gDNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Precipitation Solution is a component of the MagJET Plant Genomic DNA Kits (K2761/2762) and may be purchased separately. Provided in 16.5 mL aliquots.

Lysis Buffer for MagJET Blood gDNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET Whole Blood gDNA Kits (K2741/2742) and may be purchased separately.

PureLink™ 96 RNA Wash Buffer I (Invitrogen™)

The PureLink®RNA 96 Wash Buffer I is offered separately in bulk 5 x 1L quantities or enough to process 50 x 96mL plates . Order Filter Plates, Lysis Buffer, Wash II, and Optional Receiver plates separately.

Wash Solution I, RNA Purification (Invitrogen™)

This Wash Solution is for use with the Total RNA Chemistry System.

Total RNA Chemistry makes possible the rapid isolation of total RNA from various samples, including tissue-cultured cells, whole blood, and peripheral blood mononuclear cells (PBMCs). The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

For Research Use Only. Not for use in diagnostics procedures.

Resuspension Solution for GeneJET Plasmid Maxiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Resuspension Buffer is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately.

Lysis Buffer for MagJET RNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET RNA Kit (K2731/2732) and may be purchased separately.

Wash Solution II (concentrated) (Thermo Scientific™)

Thermo Scientific GeneJET Wash Solution II (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 180 mL aliquots.

PureLink™ Genomic Digestion Buffer (Invitrogen™)

The PureLink™ Genomic DNA Mini Kit and PureLink™ 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from a wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format. Using the PureLink™ Genomic DNA Kits, you will:
Obtain higher gDNA yields and purity - optimized spin-column membrane and buffer formulations
Save time and money - use with a large range of sample sizes (e.g., 100 µl to 1 ml blood); 96-well version does not require special equipment
Increase flexibility - one kit works with a variety of sample types

How it works
The PureLink™ Genomic DNA Mini Kit protocol improves upon the easy, familiar spin-column methods currently in use (Figures 1 and 2). Columns are newly configured to bind and release DNA more efficiently. Samples are lysed in an optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination. The chaotropic salt binding buffer allows the highest DNA binding of any column method. Powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.

For higher throughput, use the PureLink™ 96 Genomic DNA Kit (Figure 3). In this 96-well version, sample processing is performed with either a vacuum manifold or centrifugation at only 2,250 g, allowing you more flexibility in centrifuge choice. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
With the PureLink™ Genomic DNA Kits, youll get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all of your sample types - from bacteria to tissues to blood to cells (Figures 4-6). The PureLink™ 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood (Figure 7). A260/A280 and A260/A230 ratios of PureLink™ gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink™ Lysis and Wash Buffers (Figure 7).

Contents and Storage:
The PureLink™ Genomic DNA Mini Kit includes PureLink™ Genomic Spin Columns, Collection Tubes, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. The PureLink™ 96 Genomic DNA Kit includes PureLink™ Genomic Binding Plates, Wash Plates, Deep Well Plates, Foil Tape, Digestion Buffer, Lysis/Binding Buffer, Wash Buffers, Elution Buffer, Proteinase K, RNase A, and the manual. Store at room temperature. Guaranteed stable for one year when properly stored.

BloodPrep™ DNA Purification Solution (Invitrogen™)

The BloodPrep® DNA Purification Solution is a cell lysis and DNA purification solution for whole blood from any animal species, cultured cells, and buccal swabs for use with BloodPrep® DNA chemistry.

For Research Use Only. Not for use in diagnostics procedures.