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Megaplex™ PreAmp Primers, Human Pool A v2.1 (Applied Biosystems™)

For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Human Pool A v2.1 is designed to be used with Megaplex™ RT Primers, Human Pool A v2.1. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® Array MicroRNA Cards.

Megaplex™ RT Primers, Human Pool A v2.1 (Applied Biosystems™)

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A v2.1 is intended for use with the TaqMan® Array, Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ RT Primers, Rodent Pool Set v3.0 (Applied Biosystems™)

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® Array MicroRNA Card. The Rodent Pool Set v3.0 contains two Megaplex™ RT Primer Pools, Pools A and B. Collectively the pools cover 641 and 373 unique microRNAs for mouse and rat respectively. Each pool also contains 4 species specific controls. The microRNA targets represented in Rodent Pool A tend be better characterized, more broadly expressed and⁄or expressed at a higher level when compared to the Rodent Pool B while the microRNA targets represented in Rodent Pool B tend to be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to the Rodent Pool A. Rodent Pools A and B are intended for use with the TaqMan® Rodent MicroRNA Array Set v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ Primer Pools, Rodent Pools A (Applied Biosystems™)

Applied Biosystems® Megaplex™ Primer Pools, Rodent Pool A is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools A. Megaplex™ RT Primers, Rodent Pools A contains RT primers for 335 and 226 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Rodent Pool B. Rodent Pool A is intended for use with the TaqMan® Rodent MicroRNA A Array v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ Primer Pools, Human Pools B v3.0 (Applied Biosystems™)

Megaplex™ Primer Pools, Human Pools B v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Human Pools B v3.0. Megaplex™ RT Primers, Human Pool B v3.0 contains RT primers for 377 unique microRNAs and 4 controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Human Pool A. Megaplex™ Primer Pools, Human Pools B v3.0 are intended for use with the TaqMan® Array Human MicroRNA B Card v3.0, however, they can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ PreAmp Primers, Rodent Pool B v3.0 (Applied Biosystems™)

For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool B v3.0 is designed to be used with Megaplex™ RT Primers, Rodent Pool B v3.0. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.

Megaplex™ PreAmp Primers, Rodent Pool A (Applied Biosystems™)

For use when assay sensitivity is of the utmost importance, Applied Biosystems® Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pool A is designed to be used with Megaplex™ RT Primers, Rodent Pool A. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.

Megaplex™ Primer Pools, Rodent Pools Set v3.0 (Applied Biosystems™)

Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools Sets. Megaplex™ RT Primers, Rodent Pools Set collectively contains RT primers for 641 and 373 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The Megaplex™ Primer Pools, Rodent Pools Set is intended for use with the TaqMan® Rodent MicroRNA Array Set v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ RT Primers, Human Pool Set v3.0 (Applied Biosystems™)

Designed to streamline experimental workflows, Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. The Human Pool Set v3.0 contains two Megaplex™ RT Primer Pools, Pools A v2.1 and B v3.0. Collectively the pools cover 754 unique microRNAs. Additionally, each pool contains the same 4 controls. The microRNA targets represented in Human Pool A tend be better characterized, more broadly expressed and⁄or expressed at a higher level when compared to the Human Pool B while the microRNA targets represented in Human Pool B tend to be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to the Human Pool A. The Human Pool Set v3.0 is intended for use with the TaqMan® Array Human MicroRNA Card Set v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ Primer Pools, Human Pools A v2.1 (Applied Biosystems™)

Megaplex™ Primer Pools, Human Pools A v2.1 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex PreAmp Primers, Human Pools A v2.1. Megaplex™ RT Primers, Human Pool A v2.1 contains RT primers for 377 unique microRNAs and 4 controls. For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool B. Human Pool A is intended for use with the TaqMan® Array Human MicroRNA A Card v2.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ PreAmp Primers, Human Pool Set v3.0 (Applied Biosystems™)

For use when assay sensitivity of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Human Pool Set v3.0 is used with Megaplex™ RT Primers, Human Pool Set v3.0. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® Array MicroRNA Cards.

Megaplex™ RT Primers, Rodent Pool A (Applied Biosystems™)

Designed to streamline experimental workflows, Applied Biosystems® Megaplex™ RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan® MicroRNA Array. Rodent Pool A contains RT primers for 335 and 238 unique microRNAs for mouse and rat respectively plus 4 species specific controls. The microRNA targets represented in this pool tend be better characterized, more broadly expressed and⁄or expressed at a higher level compared to Human Pool A. The pool can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ PreAmp Primers, Human Pool B v3.0 (Applied Biosystems™)

For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Human Pool B v3.0 is designed to be used with Megaplex™ RT Primers, Human Pool B v3.0. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® Array MicroRNA Cards.

Megaplex™ Primer Pools, Rodent Pools B v3.0 (Applied Biosystems™)

Megaplex™ Primer Pools, Rodent Pool B v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools B v3.0. Megaplex™ RT Primers, Rodent Pool B contains RT primers for 306 and 135 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Rodent Pool A. Rodent Pool B is intended for use with the TaqMan Rodent MicroRNA B Array v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 (Applied Biosystems™)

For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. With content matched to the Megaplex™ RT Primers, Megaplex™ PreAmp Primers, Rodent Pools Set v3.0 is used with Megaplex™ RT Primers, Rodent Pools Set v3.0. Following reverse transcription using Megaplex™ RT Primers, Megaplex™ PreAmp Primers along with the TaqMan® PreAmp Master Mix uniformly amplify all microRNAs prior to quantitation by real-time PCR using TaqMan® MicroRNA Arrays.