Shop All FISH (Fluorescent in situ Hybridization) Kits

FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination (Invitrogen™)

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ ISH Cell 740 Module (Invitrogen™)

The ViewRNA™ ISH Cell 740 Module is designed to work in conjunction with both the ViewRNA™ ISH Cell Assay Kit (cat. no. QVC0001) and ViewRNA™ miRNA ISH Cell Assay Kit (QVCM0001) and contains the signal amplification and detection components for visualization of target mRNA in the 740 channel using adherent or suspension cells. The QuantiGene ViewRNA ISH Cell 740 Module is supplied with enough reagents to process 24 assays when using the 24-well chamber plate assay format and 144 assays when using 96-well optical-bottom plates. The kit is configured for processing a minimum of 6-wells per experiment when using the 24-well plate assay format. If fewer assays are processed per experiment, reagent shortages may occur.

Reported Applications:
Immunohistochemical Staining

FISH Tag™ RNA Green Kit, with Alexa Fluor™ 488 dye (Invitrogen™)

FISH Tag™ RNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ RNA Kits in two Alexa Fluor® colors or get the FISH Tag™ RNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

FISH Tag™ RNA Red Kit, with Alexa Fluor™ 594 dye (Invitrogen™)

FISH Tag™ RNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ RNA Kits in two Alexa Fluor® colors or get the FISH Tag™ RNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 594 (590/615 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

FISH Tag™ DNA Green Kit, with Alexa Fluor™ 488 dye (Invitrogen™)

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ miRNA ISH Cell Assay Kit (Invitrogen™)

The ViewRNA™ miRNA ISH Cell Assay Kit is designed for detection of one miRNA target and one mRNA target in cultured adherent or suspension cells. An additional mRNA target can be visualized when combined with the ViewRNA™ ISH Cell 740 Module.

ViewRNA is a direct fluorescence RNA in situ hybridization method that enables the simultaneous detection RNA at single copy sensitivity and single cell resolution using fluorescence microscopes or high-content imagers. Unlike traditional FISH techniques which are generally limited by high background and low sensitivity, the assay uses proprietary chemistry for the target specific probe sets (RNA FISH probes) and branch DNA signal amplification (bDNA) for detection of specific signal. The fluorescence RNA in situ hybridization assay has four main steps: sample preparation, target hybridization, signal amplification, and detection. This assay kit is designed for a 96- well optical bottom tissue culture plate or 24 well tissue culture plate (with coverslips).

The protocol for a 24 well plate assay can be requested from the Technical Support team.

Reactivity:
Armenian Hamster, Baboon, Bovine, Canine, Chicken, Chimpanzee, Cow, Donkey, Goat, Golden Syrian Hamster, Guinea Pig, Hamster, Horse, Human, Macaque, Monkey, Mouse, Olive Baboon, Pig, Pigtailed Macaque, Rabbit, Rat, Rhesus, Sheep

FISH Tag™ DNA Red Kit, with Alexa Fluor™ 594 dye (Invitrogen™)

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 594 (590/615 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ ISH Cell Assay Kit (Invitrogen™)

The ViewRNA™ ISH Cell Assay is a direct fluorescence RNA in situ hybridization method that enables the simultaneous detection of one to four RNA targets (either mRNA or non-coding RNA) at single copy sensitivity and single cell resolution using fluorescence microscopes or high-content imagers. Unlike traditional FISH techniques which are generally limited by high background and low sensitivity, the assay uses proprietary chemistry for the target specific probe sets (RNA FISH probes) and branch DNA signal amplification (bDNA) for detection of specific signal. The fluorescence RNA in situ hybridization assay has four main steps: sample preparation, target hybridization, signal amplification, and detection. This ViewRNA ISH assay kit can detect up to three RNA but can be combined with the ViewRNA™ 740 Module to add the fourth probe.

Required additional components:
10X PBS (cat. no. QVC0508)
ViewRNA™ - Detergent Solution QC (cat. No. QVC0509)
ViewRNA™ - Wash Buffer Set (cat. no. QG0507)
ViewRNA™ ISH Cell Accessory Kit (cat. no. QVC0700) intended to provide many of the required components, not supplied in the reagent kit, in order to perform the assay.
ViewRNA™ Probe Sets are not included in the assay and are designed for use with the ViewRNA ISH Cell Assays. Visit our website to view a complete listing of over 6,500 synthesized Probe Sets. By request, new Probe Sets can be designed and synthesized in less than two weeks with no additional costs.

To add the 4th RNA probe:
ViewRNA™ ISH Cell 740 Module (cat. no. QVC0200) designed to be used in conjunction with ViewRNA™ ISH Cell Assay and allows analysis of an additional RNA target in the 740 channel (AlexaFluor 750) See the package insert for a complete list of materials provided in the kit.

Reported Applications
Microscopy

FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination (Invitrogen™)

The FISH Tag™ RNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ RNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for the simultaneous localization of multiple sequence-specific RNA species in cells, tissues, and whole embryo mounts. We also offer FISH Tag™ Kits in single colors.

FISH Tag™ Kit Specifications:

• Dye (Ex/Em): Alexa Fluor® 488 (492/520 nm), Alexa Fluor® 555 (555/565 nm), Alexa Fluor® 594 (590/615 nm), Alexa Fluor® 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook. For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

ViewRNA™ ISH Cell Accessory Kit (Invitrogen™)

The ViewRNA™ ISH Cell Accessory Kit is intended to provide many of the required components, not supplied in reagent kits, in order to perform the ViewRNA™ ISH Cell Assay.

Reported Applications:
Microscopy

FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye (Invitrogen™)

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor® colors or get the FISH Tag™ DNA Multicolor Kit, which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 555 (555/565 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. PureLink® nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade® Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.