Shop All Proteinase K

Proteinase K, FS, for food safety testing (Applied Biosystems™)

Proteinase K, FS from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins. Proteinase K, FS is supplied specifically for use in food and environmental testing workflows. It is typically used in conjunction with Lysis Buffer, FS (available separately) to lyse microbial organisms in order to prepare the microbial DNA for downstream analysis by real-time PCR.

Proteinase K, FS can be used as a part of both the Pathatrix® Auto Instrument and RapidFinder™ Direct Lysis workflows.

When used as a part of the Pathatrix® Auto Instrument workflow, one bottle of Proteinase K, FS is sufficient for 1250 reactions.

When used as a part of the RapidFinder™ Direct Lysis workflow, one bottle of Proteinase K, FS is sufficient for 100 reactions.

Proteinase K, FS is supplied in 50% glycerol at a concentration of 20 mg/ml.

Unit Definition: One unit liberates 1 µmol of Folin-positive amino acids, measured as tyrosine, at 37°C, pH 7.5, using denatured bovine hemoglobin as the substrate.

Proteinase K Solution, ChIP grade (Thermo Scientific™)

Thermo Scientific Pierce ChIP-Grade Proteinase K is proteinase K diluted to 20 mg/mL from stock with an activity of ≥ 30U/mg at 35°C. This product is also sold as part of our complete Agarose ChIP Kit.

Related Products
Pierce™ Agarose ChIP Kit
Pierce™ Chromatin Prep Module
Pierce™ ChIP-Grade Protein A/G Plus Agarose

Proteinase K for MagMAX™-96 DNA Multi-Sample Kit (Invitrogen™)

This is the Proteinase K found in the MagMAX™-96 DNA Multi-Sample Kit (Cat. Nos. 4413020, 4413021, 4413022), made available separately for applications that require more proteinase K enzyme than is provided in the kit.

Proteinase K Solution (20 mg/mL) (Invitrogen™)

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Proteinase K (Fungal) (Invitrogen™)

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.

Proteinase K Powder (≥30 U/mg) (Thermo Scientific™)

Thermo Scientific Pierce Proteinase K is a protease that cleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues and is commonly used to digest and inactivate DNase and RNase in nucleic acid purification.

Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents do not interfere with the activity and may be used alongside proteinase K to inactivate calcium-dependent nucleases in DNA and RNA preparation.

Proteinase K Solution (Invitrogen™)

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Proteinase K Remains Active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g. the presence of SDS or urea)
• In the presence of metal chelating agents (e.g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Proteinase K for MagMAX™ DNA Multi-Sample Ultra Kit (Applied Biosystems™)

This is the same proteinase K found in the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. Nos. A25597, A25598), available separately in case more proteinase K is required for a particular application.

Proteinase K Solution (20 mg/mL), RNA grade (Invitrogen™)

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range—optimal activity between 6.5 and 9.5
• Under denaturing conditions—e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures—optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.

Proteinase K Powder (Invitrogen™)

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Proteinase K Remains Active:
• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g. the presence of SDS or urea)
• In the presence of metal chelating agents (e.g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Proteinase K, recombinant, PCR grade (Thermo Scientific™)

Thermo Scientific Proteinase K is a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations. It degrades proteins even in the presence of detergents. Proteinase K cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Highlights

• Ready-to-use solution
• Active in a wide range of reaction conditions

Applications

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines
• Determination of enzyme localization
• Improving cloning efficiency of PCR products

Note

• The recommended working concentration of Proteinase K is 0.05 to 1 mg/mL. The activity of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to 4 M urea
• Ca2+ protects Proteinase K against autolysis, increases the thermal stability, and has a regulatory function for the substrate binding site of Proteinase K
• Stable over a wide pH range: 4.0 to 12.5, optimum pH 7.5 to 8.0
• Optimum activity at 50 to55°C
• Rapid denaturation of enzyme occurs at temperatures above 65°C

Proteinase K (50 µg/µL) (Invitrogen™)

This Proteinase K is used in QuantiGene assays and is one of two components in the QuantiGene Sample Processing kits. Proteinase K digests proteins such as ribonucleases that could interfere with the quantitation of RNA using QuantiGene assays.