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Exosome-Human CD63 Isolation/Detection Reagent (from cell culture media) (Invitrogen™)

Exosome-Human CD63 Isolation/Detection Reagent enables the purification of CD63+ exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched cell culture samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.

• Obtain highly pure CD63+ exosomes
• "See" your sample during handling
• Easily scalable protocol
• Detect exosomes via flow cytometry, in less than 1 hour

Isolation and detection of exosomes has been a tedious, non-specific, and difficult process. Exosome – Human CD63 Isolation/Detection (from cell culture media) utilizes the well-known Dynabeads® magnetic separation technology, allowing you to easily purify pre-enriched CD63+ exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting.

Detect by Flow Cytometry
Because free exosomes alone are too small to be detected by flow cytometry, one of the major advantages of employing magnetic separation technology is that the purified, bead-bound exosomes can be easily visualized by flow cytometry. The monodispersed and relatively large Dynabeads® (4.5 µm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour.

"See" Your Sample
Not only are the superparamagnetic Dynabeads® known for their sensitivity, reproducibility, and stability, but the magnetic handling also allows you to "see" your sample due to the light brown color of the beads. When the sample tube is placed on the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and purification. In addition, the sample and bead volume can be easily scaled up or down according to your sample size or downstream applications.

Good Mixing is Critical
For successful exosome isolation it is important to use a mixer that tilts and rotates to ensure that the beads do not settle in the tube. Avoid end-over end rotation for small sample volumes (e.g., 100 µl). Please see user manual below for more guidelines.

Exosome-Streptavidin Isolation/Detection Reagent (Invitrogen™)

Exosome-Streptavidin Isolation/Detection Reagent, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes (also called extracellular vesicles and multi-vesicular bodies) from pre-enriched samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.

• Maximum flexibility with your choice of biotinylated antibody
• "See" your sample during handling
• Easily scalable protocol
• Detect exosomes via flow cytometry, in less than 1 hour

Isolation and detection of exosomes has been a tedious, non-specific, and difficult process. Exosome - Streptavidin for Isolation/Detection utilizes the well-known Dynabeads® magnetic separation technology. When combined with the biotinylated antibody of your choice, this technology allows you to easily purify pre-enriched exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting.

Detect by Flow Cytometry
Because free exosomes alone are too small to be detected by flow cytometry, one of the major advantages of employing magnetic separation technology is that the purified, bead-bound exosomes can be easily visualized by flow cytometry. The monodispersed and relatively large Dynabeads® (4.5 µm in diameter) allow for a clear and defined FFC/SSC, typically in less than 1 hour.

"See" Your Sample
Not only are the superparamagnetic Dynabeads® known for their sensitivity, reproducibility, and stability, but the magnetic handling also allows you to "see" your sample due to the light brown color of the beads. When the sample tube is placed on the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and purification. In addition, the sample and bead volume can be easily scaled up or down according to your sample size or downstream applications.

Good Mixing is Critical
For successful exosome isolation it is important to use a mixer that tilts and rotates to ensure that the beads do not settle in the tube. Avoid end-over end rotation for small sample volumes (e.g., 100 µl). Please see user manual below for more guidelines.

Exosome-Human EpCAM Flow Detection Reagent (from cell culture) (Invitrogen™)

Exosome-Human EpCAM Flow Detection Reagent (from cell culture) enables detection of CD9-positive extracellular vesicles (ECVs) such as exosomes in enriched cell culture media using flow cytometry or electron microscopy. Free exosomes alone are too small to be detected by most flow cytometry instruments. By employing Dynabeads® magnetic separation technology, exosomes can be easily visualized by flow cytometry while bound to the bead surface. The 2.7 µm Dynabeads® magnetic beads allow for a clear and defined flow analysis.

• Minimal hands-on time for specific exosome capture and analysis

• Clear and defined flow analysis in less than 1 hour hands-on time

• Study expression of specific exosome subset markers

• Protocol is easily scalable

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively captured and studied using flow cytometry and/or electron microscopy. And with Dynabeads® technology you avoid losing material due to the handling and centrifugation typically experienced with other isolation and analysis methods.

The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

Exosome-Human CD9 Isolation Reagent (from cell culture) (Invitrogen™)

Exosome-Human CD9 Isolation Reagent (from cell culture) enables isolation of CD9-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation

• Obtain a highly pure exosome subset

• Protocol is easily scalable

• Compatible with most downstream analysis

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.

Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to "see" your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

Exosome-Human CD81 Flow Detection Reagent (from cell culture) (Invitrogen™)

Exosome-Human CD81 Flow Detection Reagent (from cell culture) enables detection of CD81-positive extracellular vesicles (ECVs) such as exosomes in enriched cell culture media using flow cytometry or electron microscopy. Free exosomes alone are too small to be detected by most flow cytometry instruments. By employing Dynabeads® magnetic separation technology, exosomes can be easily visualized by flow cytometry while bound to the bead surface. The 2.7 µm Dynabeads® magnetic beads allow for a clear and defined flow analysis.

• Minimal hands-on time for specific exosome capture and analysis

• Clear and defined flow analysis in less than 1 hour hands-on time

• Study expression of specific exosome subset markers

• Protocol is easily scalable

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively captured and studied using flow cytometry and/or electron microscopy. And with Dynabeads® technology you avoid losing material due to the handling and centrifugation typically experienced with other isolation and analysis methods.

The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

Exosome-Human CD81 Isolation Reagent (from cell culture) (Invitrogen™)

Exosome-Human CD81 Isolation Reagent (from cell culture) enables isolation of CD81-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation

• Obtain a highly pure exosome subset

• Protocol is easily scalable

• Compatible with most downstream analysis

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.

Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to "see" your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

Exosome-Human EpCAM Isolation Reagent (from cell culture) (Invitrogen™)

Exosome-Human EpCAM Isolation Reagent (from cell culture) enables isolation of EpCAM-positive extracellular vesicles (ECVs) such as exosomes from enriched cell culture media. Following isolation, the specific sub-population of exosomes/ECVs can be further studied using methods like Western blotting, qPCR, or next generation sequencing.

• Minimal hands-on time for specific exosome isolation

• Obtain a highly pure exosome subset

• Protocol is easily scalable

• Compatible with most downstream analysis

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively isolated and studied. The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation. Following enrichment, specific exosomes are isolated by binding to the surface of the magnetic beads.

Dynabeads® magnetic beads are known for their sensitivity, reproducibility, and stability. Dynabeads® magnetic separation technology also allows you to "see" your sample during handling due to the color of the magnetic beads. When the sample tube is placed in the magnet, the bead-bound exosomes are pulled to the side of the tube, allowing for easy separation and buffer changes. In addition, the sample and bead volume can be easily scaled according to your sample size or downstream applications.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.

Exosome-Human CD9 Flow Detection Reagent (from cell culture) (Invitrogen™)

Exosome-Human CD9 Flow Detection Reagent (from cell culture) enables detection of CD9-positive extracellular vesicles (ECVs) such as exosomes in enriched cell culture media using flow cytometry or electron microscopy. Free exosomes alone are too small to be detected by most flow cytometry instruments. By employing Dynabeads® magnetic separation technology, exosomes can be easily visualized by flow cytometry while bound to the bead surface. The 2.7 µm Dynabeads® magnetic beads allow for a clear and defined flow analysis.

• Minimal hands-on time for specific exosome capture and analysis

• Clear and defined flow analysis in less than 1 hour hands-on time

• Study expression of specific exosome subset markers

• Protocol is easily scalable

Recent studies suggest that the exosome/ECV population consists of several subsets, possibly with different functions. Using the immuno-affinity based Dynabeads® magnetic separation technology, these subsets can be selectively captured and studied using flow cytometry and/or electron microscopy. And with Dynabeads® technology you avoid losing material due to the handling and centrifugation typically experienced with other isolation and analysis methods.

The cell culture media must first be enriched for exosomes. This can be done quickly and efficiently using Total Exosome Isolation Reagent (from cell culture media) or traditional ultracentrifugation.

Note: It is important to provide sufficient mixing to ensure that the magnetic beads do not settle in the sample tube. We recommend using a mixer which provides both tilting and rotation for optimal mixing.