Shop All Real-Time PCR Software

High Resolution Melt Software v3.0.1 Applied Biosystems™

High Resolution Melt Software v3.0.1 is an all-new and improved tool for High Resolution Melting (HRM) analysis. HRM analysis is a post-PCR analysis method used to identify variation in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by high-brightness, dsDNA-binding dyes used in conjunction with real-time PCR instrumentation that has precise temperature ramp control, advanced data capture capabilities, and access to software designed specifically for HRM analysis. High Resolution Melt Software v3.0.1 Real-Time PCR Systems, and the 7500 Fast Real-Time PCR System, and contains features that make fast and accurate HRM results more accessible than ever.

Key product features:

• Minimal user input to achieve results—software automatically applies default/saved analysis settings upon opening an experiment file
• Easy recall—new Analysis Settings Library saves settings for future experiments
• Greater accuracy—clustering algorithm accurately distinguishes control and variant genotypes
• Increased sensitivity—software allows input of expected number of clusters for difficult-to-detect SNPs (class 4)
• Ability to achieve more in one run—separate analysis of multiple assays run on a single plate
• Shortcuts in experiment setup—direct copy and paste functions with Excel; well information and plate layout clearly displayed in Plate View

Minimize User Input to Achieve Results and Easily Recall Analysis Settings
High Resolution Melt Software v3.0.1 automatically applies default or saved analysis settings upon opening an experiment file, which allows the user to achieve results with minimal input. It also includes a new Analysis Settings Library, allowing the user to save optimized settings and easily recall them for use in future experiments.

Realize Greater Accuracy and Increase Your Sensitivity
The v3.0.1 software utilizes an improved clustering algorithm that helps to accurately distinguish control and variant genotypes, and allows the user to input the expected number of clusters for increased sensitivity for difficult SNP genotyping.

Achieve More in One Run
With the v3.0.1 software, you now have the ability to conduct separate analyses of multiple assays run on a single plate.

Get Your Experiments Up and Running Fast with Shortcuts in Setup
Users of High Resolution Melt Software v3.0.1 can copy and paste directly from Excel spreadsheets to set up experiments quickly. Plate View also clearly displays well information and the plate layout to ease plate setup.

Download a FREE 30-day trial of High Resolution Melt Software v3.0.1.

See our Release Notes detailing the enhancements offered in v3.0.1.

For users of the 7500 Fast Real-Time PCR System who have already purchased High Resolution Melt Software v3.0, download the HRM v3.0.1 patch.

For HRM on the 7900HT Fast Real-Time PCR System, see High Resolution Melt Software v2.0.

Sequence Detection System v2.4 Standard Edition Applied Biosystems™

Sequence Detection System (SDS) Software v2.4.1 is the latest update in high-throughput gene expression and genotyping analysis software for use with the Applied Biosystems® 7900HT Fast Real Time PCR System.

Features of SDS Software:

• Assisted Tm Calling
• Ability to create and save analysis templates on a per detector basis for use with other plates
• Auto-analysis of plates containing analysis settings
• Improvements in amplification plot displays, including the ability to view plots by sample and target, as well as a toggle for linear/log display
• Ability to access the plate utility application from the main menu
• Ability to export the results or data of up to 50 plates at once, as well as a choice of results columns to export, using the batch export utility
• Ability to set up standard curve dilutions for AQ experiments with a wizard
• Ability to switch run documents from AQ to RQ format, and vice-versa

Standard and Enterprise Editions
The standard version of SDS Software for the 7900HT Fast Real-Time PCR System uses two automation software tools&mdashPlate Utility and Automation Controller—to fully automate high-throughput applications for gene expression and SNP genotyping, requiring minimal user intervention.

The Enterprise Edition of SDS Software is a high-throughput application for storage, organization, and analysis of large amounts of gene expression and genotyping data on the 7900HT Fast Real-Time PCR System
• Upgrade to Oracle Database 11g
• Process-based quality flags for increased quality control, problem notification, filtering, and troubleshooting
• Improved review and data group tools
• Wizard-based plate document creation
• Support for plus/minus assays
• Re-engineered SDS Automation Controller
• Ability to save plates created using the template batch utility directly to the database

The Enterprise database allows users to process up to 5,000 x 384-well plates (384 markers total), which equates to 1.92 million genotypes. Using Relative Quantitation (RQ) Manager, customers can also process 200 x 384-well plates (96 detectors with quadruplicate data points per multiplexed endogenous control) which equates to 153,600 data points.

Supporting 21 CFR Part 11 Requirements
SDS Software Enterprise Edition is the only edition of SDS Software that assists in supporting 21 CFR part 11 requirements, providing security, auditing functions, electronic signatures, and electronic record-keeping. These features eliminate traditional paper records and reduce vulnerability to signature fraud and report misfiling, which increases the speed of information exchange and data searching. The results are reduced storage costs, better data integration, and superior product quality.

For Research Use Only. Not intended for any animal, human therapeutic or diagnostic use.

QuantStudio™ 12K Flex Real-Time PCR Software Applied Biosystems™

QuantStudio™ 12K Flex Software supports the QuantStudio™ 12K Flex Real-Time PCR System. The software allows the user to open and analyze experiments generated from the QuantStudio™ 12K Flex Real-Time PCR System. The software also enables the user to set up experiments, send experiments to the instrument, control the thermal cycling process in the instrument, collect data and analyze the collected data, all in an integrated and streamlined fashion. This configuration includes a license kit with 10 keys for 10 users.

>> Download the software installer (a license key is required for use)

Features:

• QuantStudio™ System home screen enables launch of analysis applications from within application

• New Sample Tracker software for OpenArray® plates

• Easy to set up experiments for both novice and advanced users for 384-well and OpenArray® blocks

• Customizable optical filter selection for more complex applications, such as multiplexing

• QuickStart setup so you can begin a run immediately and enter plate information later

• Ability to run up to four OpenArray® plate experiments at once

• Real-time monitoring of amplification curves enables you to view a run progress (can be viewed from a remote PC as well)

• Auto-baseline and auto-threshold for simplified data analysis

• Alternative method for data analysis using a non-threshold CT calling method

• Multiple Plots view for simultaneously assessing data from four perspectives

• Automated SNP genotype calling with intuitive graphical output and quality-value assignment

• Troubleshooting flags to help you diagnose and solve problematic experiments

• Tool tips for easy identification of sample wells when viewing amplification curves or SNP genotyping plots

• Email notifications to alert you when a run has started or ended

• Easy cut-and-paste functionality

• Export easily to Microsoft® PowerPoint® software, Excel® software, or directly as a .JPEG file

AccuSEQ™ Real-Time PCR Detection Software, version 2.1.2 Applied Biosystems™

AccuSEQ Real-Time PCR Detection Software, version 2.1.2, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems 7500 Fast Real-Time PCR Instrument with 0.1-mL, 96-well plates and Windows 10 operating system.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

7500 Fast System SDS 21 CFR Part 11 Module Applied Biosystems™

The 21 CFR Part 11 Software Module for the 7500 Systems offers the flexibility of user-customizable security configuration settings to assist with 21 CFRp11 compliance.

AccuSEQ™ Real-Time PCR Detection Software, version 3.0, seat license Applied Biosystems™

This is a seat license for AccuSEQ Real-Time PCR Detection Software v3.0 (Cat. No. A40303). Seat licenses are purchased for each individual user of the software.

AccuSEQ Real-Time PCR Detection Software, version 3.0, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 0.1-mL, 96-well plates for either Fast or standard cycling.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

21 CFR Part 11 Software Module for QuantStudio™ 12K Flex System Applied Biosystems™

This software module for the QuantStudio™ 12K Flex Real-Time PCR System supports compliance with 21 CFR Part 11 FDA guidelines on electronic records security and prevents unauthorized access to the instrument. When loaded on the system, this software will:

• Authenticate each user’s log-in information
• Lock out access and require re-authentication if user is inactive for a set period of time
• Log any unauthorized attempts to access the system software in the Event Log
• Verify each user’s permission to perform predefined Controlled Activities
• Permits E-signature function for data verification

AccuSEQ™ 2.0 Real-Time PCR Software Applied Biosystems™

The AccuSEQ™ Real-Time PCR Detection Software is part of a system that performs analytical testing for impurities and contaminants during the biopharmaceutical manufacturing process. Designed to work with the 7500 Fast Real-Time PCR instrument, the software supports the SEQ family of analytical detection and quantitation assays. Security, audit, and signature capabilities help enable 21 CFR compliance. For rapid and accurate Mycoplasma detection, the AccuSEQ™ Software offers a plug-in analysis module for automated presence⁄ absence calls when using the MycoSEQ™ Mycoplasma Detection System. For host cell residual DNA quantitation, a custom module built into the main application can be used with the resDNASEQ™ CHO Residual DNA Quantitation System.

• Automated presence⁄absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Single software platform for all current and future SEQ Real-Time PCR assays
• Helps enable 21 CFR Part 11 compliance

Automated presence⁄absence calls for Mycoplasma detection
Advanced algorithms built into the AccuSEQ™ Software help enable automated presence⁄ absence calls for Mycoplasma contamination. Developed using the data interpretation guidelines for the MycoSEQ™ Mycoplasma Detection Assay, calls are made based on the Tm and derivative value of the test sample and the Ct value of the test sample and inhibition control. For in-depth review of the data, the AccuSEQ™ Software offers easy-to-use manual review tools, including a complete table of all Tm and Ct values, as well as amplification, multicomponent, and raw data plots. The AccuSEQ™ Mycoplasma SEQ experiment workflow provides a suggested plate layout, generates a reaction setup worksheet and plate layout diagram, includes pre-loaded thermal-cycling settings, and generates a summary of results and detailed reports that can be printed and saved as *.pdf, *.html, *.ppt, and *.xls files (Figure 1).

Automated residual DNA quantitation
Using the custom experiment mode, the AccuSEQ™ Software runs automated residual DNA quantitation experiments. The custom mode guides the user through the experiment and plate setup and provides easy-to-use tools for defining and setting up standards, controls, and unknowns. The user enters the number of concentrations for the standard curve, replicates, starting quantity, and serial dilution factor, and the software automatically sets up the standard curve. A customized template is available for the resDNASEQ™ CHO Residual DNA Quantitation Assay. After the run is complete, the quantities for the unknown samples, mean quantity of replicates, and standard deviation for the replicates are provided in a table format that can be exported as .pdf, .xls, or .txt formats (Figure 2).

Single software platform for all PCR assays
The AccuSEQ™ Software has been designed for use with all current and future SEQ assays through the use of plug-in modules. In combination with the 7500 Fast Real-Time PCR instrument, one platform can be used to run multiple analytical tests that are required to detect impurities and contaminants during the biopharmaceutical manufacturing process.

Helps enable 21 CFR Part 11 compliance
The AccuSEQ™ Software Security, Audit, and Electronic Signature (SAE) Manager (Figure 3) makes it easy to set up and manage the SAE requirements needed for compliance with the 21 CFR Part 11 guidance. These include:
• Security – User name and password restrictions and security policies, such as password expiration and user account suspension.
• Audit – Selection of an audit mode (Prompt⁄Silent), management of audit reasons, and sorting and printing of audit records at the experiment and system level
• Electronic Signature – Selection of an electronic signature mode (Enable⁄Disable), on-demand electronic signature, electronic signature settings, number of electronic signatures required, and electronic signature actions (Review⁄Approve⁄Sign Off) (Figure 3 and Figure 4).

For Research Use Only. Not for use in diagnostics procedures.

7300 System SDS v1.4 Software Upgrade Kit Applied Biosystems™

This software upgrades earlier versions of the 7300 SDS Software to provide maximum capability and reliability. Powerful and user-friendly, the 7300 Sequence Detection Software includes:
• Simple plate setup wizard is streamlined for each application – option to setup before or after the completing the run
• Correct plate-setup mistakes after the run without losing collected data.
• One-click graphical export to PowerPoint or JPG for top notch presentations
• Flexible system can be used as a plate reader or a regular thermal cycler if needed
• Real-time amplification monitoring and flexibility to add additional PCR cycles during a run
• Lamp-lifetime monitoring ensures highest possible instrument performance

For Research Use Only. Not for use in diagnostics procedures.

HRM Software Module for ViiA™ 7 System Applied Biosystems™

Expands the functionality of the ViiA7 system, allowing High Resolution Melting (HRM) analysis to be performed on PCR amplicons. HRM analysis can discriminate genetic variation by amplicon length, GC content, sequence variability or strand complementarity.

GenEx Software USB Key Applied Biosystems™

GenEx by Multid Analyses is software for the processing and analysis of real-time qPCR data. Its capabilities range from batch read of data files, basic data pre-processing and management to advanced cutting-edge expression profiling analyses. GenEx software provides methods to select and validate reference genes, classify samples, group genes, monitor time-dependent processes, and much more.

Basic data editing and management
GenEx software v6.1 enables the pre-processing of raw data with consistence, in the correct order, and with confidence. The pre-processing features include interplate calibration, PCR efficiency correction, optimal normalization, genomic DNA background subtraction, outlier detection, and more.

Advanced data analysis
GenEx software offers advanced statistical solutions for post-normalization data analysis. Powerful interactive presentation tools facilitate the generation of professional illustrations of even the most complex experimental designs. Current features include standard curve analyses with estimation of limit of detection (sensitivity), limit of quantification, dynamic range, repeatability and reproducibility, t-test, ANOVA and non-parametric tests with appropriate corrections and estimates of precision, and multivariate statistical tests including Hierarchical Clustering Analysis (HCA), Heatmaps, Principal Component Analysis (PCA), and Artificial Neural Networks (ANN).

GenEx Professional vs. Enterprise
GenEx Professional software provides an extensive set of pre-processing modules together with data visualization and a set of analyses including a larger number statistical analysis modules, suitable for standard curve-based analysis as well as gene expression profiling. GenEx Professional software also contains wizards for direct data import from different instruments.

GenEx Enterprise software contains all modules of GenEx Professional plus the most advanced data analyses, including Dynamic PCA, ROC curve, ANN, and Support Vector Machine (SVM). GenEx Enterprise software also provides modules for data analysis conforming to the Clinical and Laboratory Standards Institute (CLSI) standard documents.

For customers who would like to use GenEx software on multiple PCs, one at a time, GenEx licenses can be pre-installed on a USB key. Install the software on several computers and connect the USB key to the one you want to use at the time. A valid license for GenEx Professional or GenEx Enterprise software is required for purchase of a USB key.

Learn more about the features of version 6.1 at www.multid.se.
Download the user guide from www.multid.se.

AccuSEQ™ Real-Time PCR Detection Software, version 3.0 Applied Biosystems™

AccuSEQ Real-Time PCR Detection Software, version 3.0, is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument with 0.1-mL, 96-well plates for either Fast or Standard cycling.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Automated presence/absence calls for Mycoplasma detection
• Accurate quantitation of host cell line residual DNA
• Non-linear curve fitting of ProteinSEQ assay data for Protein A
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Protein quantitation
AccuSEQ software helps enable rapid and easy analysis of ProteinSEQ protein quantitation system data without the hassle of multiple exporting steps and reliance on third party software.

High Resolution Melt (HRM) Software v2.0 Applied Biosystems™

HRM Software is now available on the Applied Biosystems 7500 Fast System and the 7900HT Fast Real-Time PCR System. The 7500 Fast Real-Time PCR System delivers precise results with fast thermal cycling in a standard 96-well format. Achieve high-throughput HRM analysis with the 384-well 7900HT, the gold standard high throughput system.

High Resolution Melt (HRM) analysis is an alternative to dHPLC sequencing screening of new gene variants. The AB HRM application does not require temperature shifting, which results in a greater likelihood of identifying new homozygous mutations than methods that require temperature shifting.

• Shorten analysis time by auto-calling genotypes and automatically omitting the no template controls
• Minimize subject analysis by automatically grouping unknown variant clusters
• Enable easy data review with customizable plot views, expandable windows, and one-click color assignment to highlight curves of interest.
• Ability to analyze multiple targets (assays) on one plate

Identify more new variants, quickly and accurately
The ability to easily identify new variants is key for successful HRM applications. By eliminating the temperature shift step, the Applied Biosystems HRM solution was able to clearly distinguish homozygous variant samples from homozygous wildtype samples in 97.5% of the population, whereas the other HRM system from Competitor R was only able to distinguish them in 10% of the population. All genotypes were auto-called by the respective software packages and were not altered by the operator. Class 1 SNP (A/G), multiple technical replicates of nine DNA samples representing three genotypes: homozygous wildtype (G/G), homozygous mutant (A/A) and heterozygous (A/G).

HRM Software Module for QuantStudio™ 12K Flex System Applied Biosystems™

This software module expands the functionality of the QuantStudio™ 12K Flex software, allowing high resolution melting (HRM) analysis to be performed on PCR amplicons.

High Resolution Melt (HRM) analysis is a new, post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. Simple and fast, this method is based on PCR melt (dissociation) curve techniques and is enabled by the recent availability of improved double-stranded DNA (dsDNA)-binding dyes along with next-generation real-time PCR instrumentation and analysis software. HRM analysis can discriminate DNA sequences based on their composition, length, GC content, or strand complementarity.

GenEx Enterprise Software Applied Biosystems™

GenEx by Multid Analyses is software for the processing and analysis of real-time qPCR data. Its capabilities range from batch read of data files, basic data pre-processing and management to advanced cutting-edge expression profiling analyses. GenEx software provides methods to select and validate reference genes, classify samples, group genes, monitor time-dependent processes, and much more.

Basic data editing and management
GenEx software v6.1 enables the pre-processing of raw data with consistence, in the correct order, and with confidence. The pre-processing features include interplate calibration, PCR efficiency correction, optimal normalization, genomic DNA background subtraction, outlier detection, and more.

Advanced data analysis
GenEx software offers advanced statistical solutions for post-normalization data analysis. Powerful interactive presentation tools facilitate the generation of professional illustrations of even the most complex experimental designs. Current features include standard curve analyses with estimation of limit of detection (sensitivity), limit of quantification, dynamic range, repeatability and reproducibility, t-test, ANOVA and non-parametric tests with appropriate corrections and estimates of precision, and multivariate statistical tests including Hierarchical Clustering Analysis (HCA), Heatmaps, Principal Component Analysis (PCA), and Artificial Neural Networks (ANN).

GenEx Professional vs. Enterprise
GenEx Professional software provides an extensive set of pre-processing modules together with data visualization and a set of analyses including a larger number statistical analysis modules, suitable for standard curve-based analysis as well as gene expression profiling. GenEx Professional software also contains wizards for direct data import from different instruments.

GenEx Enterprise software contains all modules of GenEx Professional plus the most advanced data analyses, including Dynamic PCA, ROC curve, ANN, and Support Vector Machine (SVM). GenEx Enterprise software also provides modules for data analysis conforming to the Clinical and Laboratory Standards Institute (CLSI) standard documents.

For customers who would like to use GenEx software on multiple PCs, one at a time, GenEx licenses can be pre-installed on a USB key. Install the software on several computers and connect the USB key to the one you want to use at the time. A valid license for GenEx Professional or GenEx Enterprise software is required for purchase of a USB key (Cat. No. A33924).

Learn more about the features of version 6.1 at www.multid.se.
Download the user guide from www.multid.se.

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