Shop All Enzymes

Sphingosine Kinase 1a Recombinant Protein (Invitrogen™)

Mouse SPHK1a Recombinant Protein for Ctrl

Human MRP3 Vesicles (Gibco™)

Human MRP3 Vesicles are insect derived purified plasma membranes with inserted MRP3 transport protein (Multidrug Resistance-associated Protein 3, ABCC3). Our Human MRP3 Vesicles can be used to evaluate test compounds and drug interactions with MRP3 in in vitro assays. MRP3 is an efflux ATP-binding cassette (ABC) transporter that carries glucuronide conjugates and organic anions, such as methotrexate and leukotriene C4.

Use Human MRP3 Vesicles to:

• Investigate the transporter interactions of your drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high quality results with a large signal to noise ratios

Concept Behind ABC Transporter Vesicles
ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.

Clear and Reliable Results
Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these "inside-out" vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details
Human MRP3 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP3. MRP3 is is expressed on the basolateral side of small intestinal epithelial cells, where it plays a role in drug and bile acid absorption from the small intestine. It is also present on the basolateral side (vascular side) of hepatocytes, where it plays a role in xenobiotic excretion.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Fish (rainbow trout) S9 Fractions (Gibco™)

Liver S9 fractions prepared from a pool of rainbow trout (male or female, depending on lot).

Total protein concentration: 20 mg/ml

We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Custom species are available upon request.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our microsomes and subcellular fractions

BACE1 (β-Secretase) Human Protein

BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimer's disease (AD). In some cases early onset familial AD can be attributed to a "Swedish" mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1. This and other genetic and pathological evidence has led to therapeutic approaches that have focused on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase stuff.

Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit uses immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.

Features of the Mouse IgG1 Fab and F(ab')2 Preparation Kit:

High capacity—kit provides for fragmenting 0.25 to 4 mg IgG.
Complete—kit includes all reagents needed to prepare and purify antibody fragments
Enzyme-free digestion products—Immobilized Ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the proteolytic enzyme
Optimized for mouse IgG1—the kit procedure is optimized for mouse IgG1; the only effective method for this IgG species and subclass (see

Related Products
Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Pierce™ F(ab')2 Preparation Kit
Pierce™ Fab Preparation Kit

Trypsin, TPCK Treated (Thermo Scientific™)

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Features of Thermo Scientific Pierce TPCK Trypsin

• Cleaves at carboxyl side of arginine and lysine residues
• Digestion conditions: pH 7.5-9.0, 37°

Trypsin has a wide range of applications including amino acid analysis and protein sequencing, mapping and structural studies. Enzymes such as trypsin and chymotrypsin have become important tools in sequencing studies since they are highly selective in their cleavage of peptide bonds. Trypsin cleaves only those peptide bonds in which the carboxyl group is contributed by a lysine or an arginine residue, regardless of the length or amino acid sequence of the chain. Our Immobilized TPCK Trypsin can be substituted for free trypsin in many application and is advantageous because it minimizes autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the trypsin. Immobilized trypsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.

Applications:
• Removal of adherent cells from tissue culture flasks
• Preparing tryptic fragments for Edman degradation sequencing
• Immobilized trypsin can be used to purify soybean trypsin inhibitor
• Use our Pierce Trypsin, MS Grade (Part No. 90057) for mass spectrometry workflows

Trypsin is a 23.8kDa pancreatic serine endoprotease derived from trypsinogen, an inactive precursor zymogen, after enzymatic removal of an n-terminal leader sequence by enterokinase. Once some trypsin has been formed it can catalyze the conversion of more trypsinogen into its catalytically-active form. Trypsin is treated with L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotrypsin activity without affecting trypsin activity.

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Immobilized Trypsin, TPCK Treated (Agarose Resin)

Monkey (cynomolgus) Cytosol (Gibco™)

Liver cytosolic fractions prepared from a pool of male Cynomologous Monkeys.

Total protein concentration: 20 mg/ml

We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Custom species are available upon request.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our microsomes and subcellular fractions

Vivid™ Regeneration System, 100X

Vivid® Regeneration System, 100X, is a Vivid® CYP450 Screening Kit component consisting of glucose-6-phosphate and glucose-6-phosphate dehydrogenase, which converts NADP+ into NADPH, which is required to start the CYP450 reaction. Vivid® Regeneration System, 100X, is included in the Vivid® Screening Kits, but may also be purchased separately.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

CYP2E1 BACULOSOMES™ Plus Reagent, rHuman

Cytochrome P450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells infected with recombinant baculovirus containing a human CYP450 isozyme, as well as human cytochrome P450 reductase. For this particular isozyme (CYP2E1), human cytochrome b5 is also included.
• Single human P450 isozyme for detailed drug metabolism studies
• High specific activity increases dynamic range and sensitivity
• No background metabolism for clear results

Single Overexpressed Human CYP450 Isozyme
CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over Human Liver Microsomes (HLMs) in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s or other classes of drug metabolizing enzymes.

High Activity Results in Increased Sensitivity
Compound rankings based on metabolism and inhibition profiles observed with CYP450 BACULOSOMES® Plus Reagents are very similar to those seen with HLMs for most compounds tested. However, CYP450 BACULOSOMES® Plus Reagents activity and metabolic rates with most substrates is significantly higher than those seen with HLMs. This results in a broad dynamic range and high sensitivity in assays utilizing CYP450 BACULOSOMES® Plus Reagents. The high level of activity and reproducibility of the enzyme component observed in the reaction with most probe substrates makes CYP450 BACULOSOMES® Plus Reagents well suited to high-throughput screening formats.

High Signal to Noise Ratio
No background metabolism by endogenous insect CYP450s has been detected with any of the Vivid® probe substrates tested so far.

Applications: isozyme identification, DMPK analysis, isozyme-specific metabolism, and inhibition screening

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Rat (Sprague Dawley) S9 Fractions (Gibco™)

Liver S9 fractions prepared from a pool of male Sprague Dawley rats.

Total protein concentration: 20 mg/ml

We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Custom species are available upon request.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our microsomes and subcellular fractions

MEG-1 Recombinant Protein (Invitrogen™)

Human PTPN4 Recombinant Protein for Ctrl

Rat (Sprague Dawley) Cytosol (Gibco™)

Liver cytosolic fractions prepared from a pool of male Sprague Dawley rats.

Total protein concentration: 20 mg/ml

We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Custom species are available upon request.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our microsomes and subcellular fractions

PRL-1 Recombinant Protein (Invitrogen™)

Human PRL1 Recombinant Protein for Ctrl

Monkey (cynomolgus) Microsomes (Gibco™)

Liver microsomes prepared from a pool of male Cynomologous Monkeys.

Total protein concentration: 20 mg/ml

We provide liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1 and Balb-c), and trout (Oncorhynchus mykiss). Custom species are available upon request.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our microsomes and subcellular fractions

Vivid™ NADP+ , 10mM

Vivid® NADP+, 10 mM, is a Vivid® CYP450 Screening Kit component consisting of 10 mM NADP+ in 100 mM potassium phosphate, pH 8.0. Vivid® NADP+, 10 mM, is included in the Vivid® screening kits, but may also be purchased separately.

More About Vivid® CYP450 Screening Kits
Vivid® CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid® CYP450 Screening Kits include Vivid® Substrate, Vivid® Fluorescent Standard, reaction buffer, Vivid® Regeneration System, NADP+, and CYP450 BACULOSOMES® Plus Reagent. Vivid® Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES® Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES® Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

>> View all Vivid® CYP450 Screening Kits

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.