Shop All Primary Cell Isolation Kits & Reagents

Pierce™ Mouse Embryonic Fibroblast Isolation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

Features of the Mouse Embryonic Fibroblast Isolation Kit:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
Functional – cultured fibroblasts express the appropriate biochemical markers

Includes:
• Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation

The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure. The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts. In addition, MEF isolated and cultured with the Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability.

Applications:
• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• IHC and IF studies

Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods. Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function. After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines. They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].

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An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Related Products
Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

Pierce™ Primary Neuron Isolation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons from embryonic cortical and hippocampal tissue from mouse and rat.

Features of the Primary Neuron Isolation Kit:

Optimized—procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield—provides a 2-fold increase in yield compared to do-it-yourself methods
Viability—greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
Functional—cultured neurons contain processes indicative of complete differentiation and functionality

The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.

Includes:
• Complete kit includes Neuronal Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), Neuronal Culture Medium, Neuronal Culture Media Supplement, Glutamine Supplement, and Neuronal Growth Supplement

Requires:
• Fresh embryonic mouse or rat tissue sample, heat-inactivated fetal bovine serum (FBS), poly-D-lysine treated culture dishes

Applications:
• Neuronal cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery, neurotoxicity testing, and predictive disease modeling

The Pierce Primary Neuron Isolation Kit has been optimized to provide excellent cell yield and to sustain cells at greater than 95% viability. The neurons are appropriately polarized, develop extensive axonal and dendritic arbors, express neuronal and synaptic markers, and form numerous, functional synaptic connections with one another. They can be used as a model system for molecular and cellular biology studies of neuronal development and function, especially for visualizing the subcellular localization of endogenous or expressed proteins, imaging protein, neuronal polarity, and dendritic growth and synapse formation [1].

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

Neuronal Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for the isolation of primary cortical and hippocampal neurons from mouse/rat embryonic brain tissue and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. When used with Cardiomyocyte Isolation Enzyme 2 (with thermolysin), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Papain Information:

Source:Papaya latex
Molecular mass: 23kDa
Optimal pH: 6.0-7.0

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Pierce™ Primary Cardiomyocyte Isolation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of the Primary Cardiomyocyte Isolation Kit:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

Cardiomyocyte Isolation Enzyme 2 (with thermolysin) is a tissue-specific dissociation reagent for the isolation of primary cardiomyocytes from neonatal heart tissue from mouse/rat and is a component of the Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit (Product No. 88281).

Thermolysin is a thermostable extracellular metalloendopeptidase. When used with Cardiomyocyte Isolation Enzyme 1 (with papain), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Thermolysin Information:
Source: Bacillus thermoproteolyticus rokko
Molecular mass: 34.6kDa

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

Neuronal Culture Media Supplement is a proprietary reagent that has been formulated for optimal growth of primary mouse and rat neurons. The supplement has been formulated for use with Neuronal Culture Medium (Product No. 88283) and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for isolation of MEFs from mouse embryonic tissue and is a component of the Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit.

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. At its optimal concentration, this reagent ensures a high yield of viable and functional fibroblasts.

More Product Data
An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Related Products
Pierce™ Mouse Embryonic Fibroblast Isolation Kit

Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

The Thermo Scientific Pierce Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ is ideal for gently washing primary cells after digestion of the connective tissue. The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of the Thermo Scientific Pierce Hanks' Balanced Salt Solution (HBSS):

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
DMEM for Pierce™ Primary Cell Isolation Kits

DMEM for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

The Thermo Scientific Pierce DMEM for Primary Cell Isolation is classical Dulbecco's Modified Eagle's Medium - High Glucose, which has been shown to be ideal for the isolation of primary cells, including Cardiomyocytes. The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat.

Features of Thermo Scientific Pierce DMEM for Primary Cell Isolation:

Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use
Time-saving – isolation protocol requires less than 2 hours compared to up to 21 hours by other methods
Yield – provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits
Viability – provides higher viability than do-it-yourself methods and other commercial kits
Functional – cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity

Includes:
• Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement

Applications:
• Cardiomyocyte cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery and predictive disease modeling

The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes. The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods. Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components.

Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells. The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes. The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours.

Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods. The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function (beating activity, see video), thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds.

   

More Product Data
One-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts

Related Products
Pierce™ Primary Cardiomyocyte Isolation Kit
Cardiomyocyte Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits
Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X) for Pierce™ Primary Cell Isolation Kits
Hanks' Balanced Salt Solution (HBSS) without Ca2+, Mg2+ for Pierce™ Primary Cell Isolation Kits

Cancer Cell Isolation Kit (Invitrogen™)

The Cancer Cell Isolation Kit provides a simple, cost-effective method for isolating cancer cells from biopsy samples. Cancer cells purified using this technique can be used immediately for a wide range of applications, including biomarker identification, microarray hybridization, and genetic analysis. Moreover, the whole cell isolation procedure is so gentle that cells can be used to establish primary cell lines.

• Purify cancer cells from fresh biopsy samples
• Establish primary cell lines or use for biochemical assays
• Ideal for proteomics applications

Purify any population of cancer cells
The ability to obtain uncontaminated cancer cell samples is one of the major bottlenecks in the study of tumor development and cancer biology. Tumor biopsies from cancer patients and animal tumor models often contain a heterogeneous population of cells that include normal tissue, blood, and cancer cells. This mixed population makes diagnosis and valid experimental conclusions difficult to obtain and interpret.

Typically, cancer cells are isolated from biopsy samples via microdissection, a time-consuming technique that requires costly equipment and surgical precision. Another conventional method is immunoprecipitation with antibodies specific to each cancer cell type. Unlike these techniques, the USB Cancer Cell Isolation Kit requires no specialized skill set and no expensive equipment, and can be used to isolate many cancer cell types.

Simple, cost-effective technique
The cancer cell isolation method only takes a couple of hours, and involves just a few rounds of incubation, washing, and centrifugation. Using the same amount of starting biopsy material required for conventional methods, this technique can be used to isolate a much greater quantity of cancer cells.

Cancer cells purified using the Cancer Cell Isolation Kit can be used directly for proteomics applications such as identification of biomarkers or for RNAbased applications such as microarray hybridization and genetic analysis. Cells can also be used to purify proteins to test antibody-based cancer therapies. Alternatively, cells can be used to establish primary cell lines.

To prepare nuclear extracts from isolated cancer cells, please see the Nuclear Extraction Kit, PN AY2002.

Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits (Thermo Scientific™)

The Thermo Scientific Pierce Primary Neuron Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary neurons form embryonic cortical and hippocampal tissue from mouse and rat.

Features of The Thermo Scientific Pierce Primary Neuron Isolation Kit:

Optimized—procedure and reagents optimized for viability, yield, purity, and ease-of-use
Yield—provides a 2-fold increase in yield compared to do-it-yourself methods
Viability—greater than 95% of isolated cells remain viable in culture from Day 1 to Week 4
Functional—cultured neurons contain processes indicative of complete differentiation and functionality

Includes:
• Complete kit includes Neuronal Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), Neuronal Culture Medium, Neuronal Culture Media Supplement, Glutamine Supplement, and Neuronal Growth Supplement

Requires:
• Fresh embryonic mouse or rat tissue sample, heat-inactivated fetal bovine serum (FBS), poly-D-lysine treated culture dishes

Applications:
• Neuronal cell differentiation
• Immunohistochemistry (IHC)
• Functional and biochemical assays
• Biologically relevant system for preclinical drug discovery, neurotoxicity testing, and predictive disease modeling

The Primary Neuron Isolation Kit has been designed to obtain high viability and purity of primary embryonic neurons using validated reagents and an optimized procedure for enzymatic digestion and culturing. The kit contains tissue-specific dissociation enzymes, media formulated specifically for neuronal cell culture, and cell culture supplements that promote the isolation and growth of functional neurons. The validated protocol and reagents produce highly pure primary neurons for either short- or long-term culture, and the kit is designed for use by both experienced and non-experienced users.

The Pierce Primary Neuron Isolation Kit has been optimized to provide excellent cell yield and to sustain cells at greater than 95% viability. The neurons are appropriately polarized, develop extensive axonal and dendritic arbors, express neuronal and synaptic markers, and form numerous, functional synaptic connections with one another. They can be used as a model system for molecular and cellular biology studies of neuronal development and function, especially for visualizing the subcellular localization of endogenous or expressed proteins, imaging protein, neuronal polarity, and dendritic growth and synapse formation [1].

More Product Data
An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuron Isolation Enzyme (with papain) for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent