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Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade Thermo Scientific™

Thermo Scientific Pierce Trifluoroacetic Acid (TFA), Sequencing grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Features of Trifluoroacetic Acid (TFA), Sequencing grade:

High purity and exceptional clarity—allows sensitive, nondestructive peptide detection at low UV wavelengths in reverse-phase HPLC protein and peptide separation systems
High-performance packaging—TFA packaged under nitrogen in amber glass ampules or bottles with protective PTFE-lined fluorocarbon caps to ensure TFA integrity
Economical convenience—Choose the TFA format that works best for your application. In just a few seconds, 1 ml ampules can be used to prepare 1 liter of fresh 0.1% v/v trifluoroacetic acid solution for the mobile phase in reverse-phase chromatography

Applications of Trifluoroacetic Acid (TFA), Sequencing grade:
• Ion pair reagent for reverse-phase HPLC
• Protein/peptide sequencing
• Protein/peptide solubilizing agent
• Solid-phase peptide synthesis
• Amino acid analysis
• Making 0.1% solutions of trifluoroacetic acid (w/v vs. v/v)

Trifluoroacetic acid (TFA) is the most commonly used ion pairing agent for use in reverse-phase HPLC peptide separations because it sharpens peaks and improves resolution, is volatile and easily removed, has low absorption within detection wavelengths. It is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Making 0.1% Trifluoracetic Acid Solutions:
For complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptides that would otherwise overlap. Trifluoroacetic acid is the most frequently used modifier for peptide separations in reverse-phase HPLC. The TFA concentration usually specified is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.

Trifluoroacetic acid concentration can and should be specified as either 'w/v' (weight/volume), or as 'v/v' (volume/volume). The w/v specification designates that the TFA is to be weighed and added to a volume of mobile phase (e.g. 0.1% TFA w/v requires one gram of TFA per liter). The v/v specification designates that the TFA is to be measured by volume (e.g. 0.1% TFA v/v requires one mL of TFA per liter).

Because the density of trifluoroacetic acid is 1.53 g/ml the difference between 0.1% TFA (w/v) and 0.1% TFA (v/v) is more than 50%. For the sake of reproducibility, it is essential for authors of a method to specify, and for users of a method to know, whether the TFA concentration is given as 'w/v' or 'v/v'.

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Pierce™ Trifluoroacetic Acid (TFA), Sequencing grade

Pierce™Pyridine, LC-MS Grade Thermo Scientific™

Perform liquid chromatography (HPLC) and mass spectrometry (LC-MS) applications with 99.5%-pure sequencing-grade pyridine solvent with low UV-absorptivity.

Pierce™ Triethylamine (TEA), Sequencing Grade Thermo Scientific™

Thermo Scientific Pierce Triethylamine (TEA) is a high-purity solvent that is used in certain HPLC protocols, such as weak anion exchange, to resolve certain tryptic peptides on a reverse-phase column.

Features of Thermo Scientific Pierce Triethylamine (TEA):

• Greater than 99.5% triethylamine purity, allowing sensitive peptide detection at low UV wavelengths in reverse-phase HPLC peptide separation systems
• Packaged in amber glass bottles with protective PTFE-lined fluorocarbon caps for reagent integrity

Triethylamine is an ion-pairing reagent that alters selectivity in reverse-phase HPLC separations. By pairing with peptides, it effectively sharpens peaks, resulting in improved peak resolution. Pierce Triethylamine has low UV absorbance properties to provide sensitive detection across all wavelengths when used as an ion-pair reagent in HPLC separations. The reagent is designed and tested to meet the special requirements for peptide sequencing and analysis.

Pierce Triethylamine is specially purified and each lot is tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Pierce™ 0.1% Trifluoroacetic Acid (v/v) in Water, LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce 0.1% Trifluoroacetic Acid (v/v) in Water, LC-MS Grade is a preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.

Features of 0.1% Trifluoroacetic Acid (v/v) in Water, LC-MS Grade:

Validated—tested more than 20 ways to ensure maximal LC-MS sensitivity
Purified—processed, filtered and sealed to extend LC-MS column life
Convenient—packaged to eliminate variability and reduce handling

0.1% Trifluoroacetic Acid (TFA) in water is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance, providing the most sensitive detection across wavelengths and prolonging equipment life. The chromatography- and spectrometry-grade 0.1% TFA in water is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen with PTFE-lined fluorocarbon caps for ultimate protection.

For complex separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve separations that would otherwise overlap. TFA is the most frequently used modifier for separations in reverse-phase HPLC. The usual specified TFA concentration is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.

Pierce™ Dimethylsulfoxide (DMSO), Sequencing Grade Thermo Scientific™

Thermo Scientific Pierce Dimethylsulfoxide (DMSO) is a sequencing-grade preparation with high purity for use in traditional amino acid analyzers and other high-volume applications.

This sequencing-grade dimethylsulfoxide is >99.5% pure DMSO. The economically priced 950 mL bottles are an excellent choice for protein and peptide applications that requiring large volumes of the water-miscible solvent. (For small-volume needs, consider the related 50 mL bottles of DMSO, Cat. No. 85190.) The purity test ensures that preparation is essentially devoid of amines. The formulation was originally developed for peptide sequencing applications with amino acid analyzers, which required high purity solvent and an affordable price. This DMSO is packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps for ultimate protection.

Features of DMSO:

• High-purity (low amine) solvent for various protein, peptide and amino acid applications
• Packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps
• Suitable for HPLC detection, although it is not specifically tested for low UV-absorptivity

General properties of DMSO
• Alternative names: Methylsulfinylmethane, methyl sulfoxide
• Molecular formula: C2H6SO
• Molecular weight: 78.13
• Density: 1.101 g/mL
• CAS number: 67-68-5

Specifications of Thermo Scientific Pierce DMSO
• Particulates: Free from particulate matter
• Purity: > 99.5%
• Water content: < 0.2%

This DMSO is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

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Pierce™ Dimethylsulfoxide (DMSO), LC-MS Grade

Pierce™ Formic Acid Thermo Scientific™

Thermo Scientific Pierce Formic Acid is high-purity solvent supplied in bottles as a convenient, contamination-free alternative for preparing elution solvents for HPLC separations of protein and peptides.

General properties of formic acid
• Molecular formula: HCOOH (CH2O2)
• Molecular weight: 46.02
• Density: 1.22 g/mL
• CAS number: 64-18-6
• Refractive index: 1.3701–1.3721(20°C)
• Flash point: 69°C
• Freezing point: 8°C

Specifications of Thermo Scientific Pierce Formic Acid
• Visual: Clear liquid, free of particulate matter
• Identity (IR): Must show only peaks characteristic for the compound
• Purity: > 99%
• Refractive index: 1.3701 to 1.3721 (20°C, 589 nm)

Pierce Formic Acid is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Formic acid is a common component of reverse-phase mobile phases that provide protons for LC/MS analysis. The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation. Unlike trifluoroacetic acid (TFA), formic acid is not an ion-pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile-phase component.

Pierce™ Acetonitrile (ACN), LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce Acetonitrile (ACN) is an LC/MS-grade preparation with high purity and low UV-absorptivity that makes it suitable for HPLC and mass spectrometry applications.

Features of Acetonitrile (ACN), LC-MS Grade:

• 37 quality tests ensure low, stable baselines and lot-to-lot consistency
• Low UV absorbance yields low baselines and high detection sensitivity
• Low impurities protects columns and simplifies analysis by eliminating extraneous peaks

Thermo Scientific Pierce LC/MS Grade Acetonitrile is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance to provide you with the most sensitive detection across all wavelengths. Pierce Acetonitrile is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen atmosphere with PTFE-lined fluorocarbon caps for ultimate protection.

Pierce LC/MS Grade Acetonitrile is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment. These Acetonitrile specifications also meet ACS standards.

Pierce™Water, LC-MS Grade Thermo Scientific™

Thermo Scientific™ Pierce™ Water, LC-MS Grade is an ultrapure, LC-MS grade preparation with low UV-absorptivity that makes it suitable and reliable for use in HPLC and mass spectrometry applications.

Pierce™ Water, LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce Water, LC-MS Grade is an ultrapure, LC-MS grade preparation with low UV-absorptivity that makes it suitable and reliable for use in HPLC and mass spectrometry applications.

Features of Water, LC-MS Grade:

• Purity and quality validated with 30 tests for use in LC-MS applications
• 0.2-micron filtered, packed in solvent-rinsed amber glass bottles with Teflon TFE-lined fluorocarbon caps for ultimate protection.
• Sealed under nitrogen atmosphere to eliminate absorption of unknown atmospheric gases
• High lot-to-lot consistency and reliability

Pierce™ Heptafluorobutyric Acid (HFBA), HPLC Grade Thermo Scientific™

Thermo Scientific Pierce Heptafluorobutyric Acid (HFBA), HPLC Grade is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

Features of Heptafluorobutyric Acid (HFBA), HPLC Grade:

Greater than 99.5% purity—allows sensitive, nondestructive peptide detection at low UV wavelengths in reverse-phase HPLC protein and peptide separation systems
High-performance packaging—HFBA packaged under nitrogen in amber glass ampules or bottles
Economical convenience—in just a few seconds, a 1 mL ampule can be used to prepare 1 liter of fresh 0.1% heptafluorobutyric acid solution for the mobile phase in reverse-phase chromatography

Applications:
• Ion pair reagent for reverse-phase HPLC
• Protein/peptide sequencing
• Protein/peptide solubilizing agent
• Solid-phase peptide synthesis
• Amino acid analysis

Pierce HFBA is manufactured to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment. The solution is tested for overall purity, chain-length purity, water content, sulfate content and absorptivity at three different UV-wavelengths. The liquid reagent is offered in convenient 1 mL ampules and 100 mL bottles.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Pierce™ Heptafluorobutyric Acid (HFBA), Sequencing Grade

Pierce™ 0.1% Formic Acid (v/v) in Acetonitrile, LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce 0.1% Formic Acid (v/v) in Acetonitrile is an LC/MS-grade preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.

Features of 0.1% Formic Acid (v/v) in Acetonitrile:

Validated—tested more than 20 ways to ensure maximal LC/MS sensitivity
Purified—processed, filtered and sealed to extend LC/MS column life
Convenient—packaged to eliminate variability and reduce handling

This 0.1% Formic Acid in Acetonitrile is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance, providing the most sensitive detection across wavelengths and prolonging equipment life. The chromatography- and spectrometry-grade 0.1% FA in acetonitrile is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen with PTFE-lined fluorocarbon caps for ultimate protection.

This preparation of Thermo Scientific 0.1% Formic Acid (v/v) in Acetonitrile is specially purified and tested to the highest specifications to ensure the integrity of chromatography data, maximize assay sensitivity and prolong the life of equipment.

Formic acid is a common component of reverse-phase mobile phases that provide protons for LC/MS analysis. The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation. Unlike trifluoroacetic acid (TFA), formic acid is not an ion-pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile-phase component.

Pierce™ Dimethylsulfoxide (DMSO), LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce Dimethylsulfoxide (DMSO) is a sequencing-grade preparation with high purity for use in traditional amino acid analyzers and other high-volume applications.

This sequencing-grade dimethylsulfoxide is >99.5% pure DMSO. The 50 mL bottles provide convenience and minimize waste for small dissolution and reaction volumes. (For protein and peptide applications that require large volumes of the water-miscible solvent, consider the related, economically-priced 950 mL bottles of DMSO, Cat. No. 20688.) The purity test ensures that preparation is essentially devoid of amines. The formulation was originally developed for peptide sequencing applications with amino acid analyzers, which required high purity solvent and an affordable price. This DMSO is packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps for ultimate protection.

Features of DMSO:

• High-purity (low amine) solvent for various protein, peptide and amino acid applications
• Packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps
• Suitable for HPLC detection, although it is not specifically tested for low UV-absorptivity

General properties of DMSO
• Alternative names: Methylsulfinylmethane, methyl sulfoxide
• Molecular formula: C2H6SO
• Molecular weight: 78.13
• Density: 1.101 g/mL
• CAS number: 67-68-5

Specifications of Thermo Scientific Pierce DMSO
• Particulates: Free from particulate matter
• Purity: > 99.5%
• Water content: < 0.2%

This DMSO is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Related Products
Pierce™ Dimethylsulfoxide (DMSO), Sequencing Grade

Pierce™ 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade is a preparation with high purity and low UV-absorptivity that is ideal for HPLC and mass spectrometry applications.

Features of 0.1% Trifluoroacetic Acid (v/v) in Acetonitrile, LC-MS Grade:

Validated—tested more than 20 ways to ensure maximal LC-MS sensitivity
Purified—processed, filtered and sealed to extend LC-MS column life
Convenient—packaged to eliminate variability and reduce handling

0.1% Trifluoroacetic Acid (TFA) in Acetonitrile is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance, providing the most sensitive detection across wavelengths and prolonging equipment life. The chromatography- and spectrometry-grade 0.1% TFA in acetonitrile is 0.2-micron filtered, packaged in solvent-rinsed amber glass bottles and sealed under a nitrogen with PTFE-lined fluorocarbon caps for ultimate protection.

For complex separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve separations that would otherwise overlap. TFA is the most frequently used modifier for separations in reverse-phase HPLC. The usual specified TFA concentration is 0.1%. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make TFA concentrations the same.

Pierce™ Dimethylformamide (DMF), Sequencing grade Thermo Scientific™

Thermo Scientific Pierce Dimethylformamide (DMF) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods.

This sequencing-grade dimethylformamide is specially purified by a proprietary method and tested to ensure lot-to-lot consistency with a low UV absorbance to provide you with the most sensitive detection across all wavelengths. DMF is packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps for ultimate protection.

Features of dimethylformamide:

• Highly purified and tested to ensure reliable performance in HPLC, mass spectrometry and sequencing applications
• Packaged in solvent-rinsed amber glass bottles and sealed with PTFE-lined fluorocarbon caps

General properties of DMF
• Alternative names: N,N-Dimethylformamide, N-Formyldimethylamine
• Molecular formula: C3H7ON
• Molecular weight: 73.09
• Density: 0.945 g/mL
• CAS number: 68-12-2

Specifications of Thermo Scientific Pierce DMF
• Particulates: Free from particulate matter
• Purity: >99.5%
• Water content: < 0.1%
• Peroxides: No peroxides detected
• Tollen's test: No aldehydes detected
• Ninhydrin positives: A570 < 0.05 above blank
• UV cutoff: < 270 nm

Pierce sequencing-grade DMF is specially purified and tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.

Pierce™ Pyridine, LC-MS Grade Thermo Scientific™

Thermo Scientific Pierce Pyridine (PY) is a sequencing-grade preparation with high purity and consistent absorptivity properties for use in HPLC and mass spectrometry methods.

Features of Pyridine (PY) :

• Water-miscible organic solvent for a variety of molecular and protein biology methods
• High purity, low water, and no peroxides or aldehydes
• Ninhydrin test ensures low amines
• UV-absorption test confirms suitability for HPLC detection
• Liquid reagent packaged by mass in amber glass bottles

Pierce pyridine is specially purified and each lot is tested to the highest specifications to ensure the integrity of your data, maximize sensitivity in your assay and to prolong the life of your equipment.
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