Shop All Pegylation Reagents

CA(PEG)24 Carboxy-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce CA(PEG)24 is a pegylated amino acid of the form carboxy-PEG-amine, containing 24 polyethylene glycol units, and is useful for a variety of surface- and molecule-pegylation applications.

CA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with carboxy (-COOH) and amino (-NH2) termini. The unbranched, hydrophilic, discrete-length molecules have the form Carboxy-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide specific targets for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Features of CA(PEG)n Compounds

• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling or crosslinking involving primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

Applications of PEGylation
• PEGylate carboxyl or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment
• Derivatize or crosslink carboxyls or primary amines using EDC and Sulfo-NHS
• Crosslink amino terminus to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink amino terminus to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)8 Carboxy-PEG-Amine Compound
CA(PEG)12 Carboxy-PEG-Amine Compound

MA(PEG)4 Methyl-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce MA(PEG)4 is a methyl- and amine-terminated polyethylene glycol reagent that is useful for a variety of surface-modification and molecule-pegylation applications.

Features of MA(PEG)4:

• Fully characterized PEGylation reagent with defined PEG chain length; discrete molecular weight for consistency of performance in protein-modification applications
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling of primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

MA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers and terminal methyl (-CH3) and amino (-NH2) groups. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Applications of PEGylation:
• PEGylate carboxylate or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment:
• Conjugate to carboxyl groups using EDC and Sulfo-NHS
• Crosslink to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent
• Attach to oxidized carbohydrate groups (aldehydes) by reductive amination

Why PEGylate a protein or peptide?
Methyl-capped PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in a biological application, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds:
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are a heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
MA(PEG)8 Methyl-PEG-Amine Compound
MA(PEG)12 Methyl-PEG-Amine Compound
MA(PEG)24 Methyl-PEG-Amine Compound

MT(PEG)4 Methyl-PEG-Thiol Compound (Thermo Scientific™)

Thermo Scientific Pierce MT(PEG)4, or methyl-PEG4-thiol, is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Features of MT(PEG)4:

Metal-binding—terminal monodentate thiol reacts spontaneously with gold surfaces through dative binding
Methyl—terminal methyl group is unreactive, the primary function of this compound being to coat quantum dot and other surfaces to reduce nonspecific binding in assays
Polyethylene glycol—PEG groups are flexible, non-immunogenic, hydrophilic, and significantly reduce nonspecific binding of surfaces for protein methods
Spacer arm—four-unit PEG spacer makes compound nearly 16 angstroms long

MT(PEG)4 is monodentate thiol-terminated and methyl pegylation reagent. The PEG compound has a defined molecular weight and spacer length and is useful for modifying surfaces such quantum dots, self-assembled monolayers and magnetic particles. Functionalization of solid surfaces with polyethylene glycol spacers such as this one significantly reduces nonspecific protein binding.

The use of MT(PEG)4 with CT(PEG)12 in surface modification can form a hydrophilic "lawn" of methyl ether-terminated PEGs with periodic exposed carboxylic acid-containing PEGs. The exposed carboxylic acid groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Related Products
ML(PEG)4 Methyl-PEG-Lipoamide Compound

TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent (Thermo Scientific™)

Thermo Scientific Pierce TMS(PEG)12 is a three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent pegylation of primary amines (e.g., lysines) on proteins or assay surfaces.

Features of TMS(PEG)12:

• NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome

TMS(PEG)12 is the abbreviation for a branched trimethyl (TM) and succinimide ester (S) derivative of polyethylene glycol (PEG) for efficient and specific modification of primary amines. Each methyl-terminated PEG (mPEG) branch contains 12 ethylene glycol units. The three branches are attached to a 4-unit PEG stem that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at the distal end. The NHS ester is spontaneously reactive with primary amines (—NH2), providing for efficient PEGylation of proteins, peptides and other amine-containing molecules or surfaces.

PEGylation Applications:
• PEGylate amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

TMS(PEG)12 is specially synthesized as a homogeneous compound of discrete chain length and defined molecular weight. By contrast, typical preparations of PEG compounds are heterogeneous mixtures composed of multiple chain lengths and a range of molecular weights.

Related Products
MS(PEG)24 Methyl-PEG-NHS-Ester Reagent

MM(PEG)24 Methyl-PEG-Maleimide Reagent (Thermo Scientific™)

Thermo Scientific Pierce MM(PEG)24 is a methyl-terminated, polyethylene glycol compound (24 PEG units) activated with a maleimide group for covalent pegylation of sulfhydryls on proteins (e.g., cysteines) or assay surfaces.

Features of MM(PEG)24:

• Maleimide-activated for efficient PEGylation of sulfhydryl groups at pH 6.5-7.5; reaction of maleimide group results in formation of stable, irreversible thioether bonds
• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided with 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome

PEGylation Applications:
• PEGylate amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length mPEG-NHS Ester Compounds:
These reagents are specially synthesized as homogeneous compounds of discrete chain length and defined molecular weight. As such, they enable precise control and optimization of surface protein modification experiments. By contrast, typical preparations of PEG compounds are heterogeneous mixtures composed of multiple chain lengths and a range of molecular weights.

Related Products
MM(PEG)12 Methyl-PEG-Maleimide Reagent

CA(PEG)12 Carboxy-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce CA(PEG)12 is a pegylated amino acid of the form carboxy-PEG-amine, containing 12 polyethylene glycol units, and is useful for a variety of surface- and molecule-pegylation applications.

CA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with carboxy (-COOH) and amino (-NH2) termini. The unbranched, hydrophilic, discrete-length molecules have the form Carboxy-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide specific targets for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Features of CA(PEG)n Compounds

• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling or crosslinking involving primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

Applications of PEGylation
• PEGylate carboxyl or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment
• Derivatize or crosslink carboxyls or primary amines using EDC and Sulfo-NHS
• Crosslink amino terminus to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink amino terminus to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)8 Carboxy-PEG-Amine Compound
CA(PEG)24 Carboxy-PEG-Amine Compound

MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific™)

Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) activated as NHS esters for covalent pegylation of primary amines on proteins (e.g., lysines) or assay surfaces.

Features of MS(PEG)4:

• NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome

MS(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with methyl (—CH3) and amine-reactive NHS-ester groups at opposite ends. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-NHS Ester, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The N-hydroxysuccinimide (NHS) ester is spontaneously reactive with primary amines (—NH2), providing for efficient PEGylation of proteins, peptides and other amine-containing molecules or surfaces.

PEGylation Applications:
• PEGylate amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length mPEG-NHS Ester Compounds
These reagents are specially synthesized as homogeneous compounds of discrete chain length and defined molecular weight. As such, they enable precise control and optimization of surface protein modification experiments. By contrast, typical preparations of PEG compounds are heterogeneous mixtures composed of multiple chain lengths and a range of molecular weights.

Related Products
MS(PEG)8 Methyl-PEG-NHS-Ester Reagent
MS(PEG)12 Methyl-PEG-NHS-Ester Reagent
MS(PEG)24 Methyl-PEG-NHS-Ester Reagent

MA(PEG)24 Methyl-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce MA(PEG)24 is a methyl- and amine-terminated polyethylene glycol reagent that is useful for a variety of surface-modification and molecule-pegylation applications.

Features of MA(PEG)24:

• Fully characterized PEGylation reagent with defined PEG chain length; discrete molecular weight for consistency of performance in protein-modification applications
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling of primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

MA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers and terminal methyl (-CH3) and amino (-NH2) groups. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Applications of PEGylation:
• PEGylate carboxylate or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment:
• Conjugate to carboxyl groups using EDC and Sulfo-NHS
• Crosslink to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent
• Attach to oxidized carbohydrate groups (aldehydes) by reductive amination

Why PEGylate a protein or peptide?
Methyl-capped PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in a biological application, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds:
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are a heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
MA(PEG)4 Methyl-PEG-Amine Compound
MA(PEG)8 Methyl-PEG-Amine Compound
MA(PEG)12 Methyl-PEG-Amine Compound

MA(PEG)8 Methyl-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce MA(PEG)8 is a methyl- and amine-terminated polyethylene glycol reagent that is useful for a variety of surface-modification and molecule-pegylation applications.

Features of MA(PEG)8:

• Fully characterized PEGylation reagent with defined PEG chain length; discrete molecular weight for consistency of performance in protein-modification applications
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling of primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

MA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers and terminal methyl (-CH3) and amino (-NH2) groups. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Applications of PEGylation:
• PEGylate carboxylate or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment:
• Conjugate to carboxyl groups using EDC and Sulfo-NHS
• Crosslink to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent
• Attach to oxidized carbohydrate groups (aldehydes) by reductive amination

Why PEGylate a protein or peptide?
Methyl-capped PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in a biological application, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds:
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are a heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
MA(PEG)4 Methyl-PEG-Amine Compound
MA(PEG)12 Methyl-PEG-Amine Compound
MA(PEG)24 Methyl-PEG-Amine Compound

CT(PEG)12 Carboxy-PEG-Thiol Compound (Thermo Scientific™)

Thermo Scientific Pierce CT(PEG)12, or carboxy-PEG12-thiol, is a carboxyl- and sulfhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

CT(PEG)12 is monodentate thiol-terminated and carboxylic acid pegylation reagent. The PEG compound has a defined molecular weight and spacer length and is useful for modifying surfaces such quantum dots, self-assembled monolayers and magnetic particles. Functionalization of solid surfaces with this polyethylene glycol reagent provides carboxylate "handles" for protein immobilization via EDC crosslinking and significantly reduces nonspecific protein binding and provides.

Features of carboxy-PEG12-thiol:

Metal-binding—terminal monodentate thiol reacts spontaneously with gold surfaces through dative binding
Carboxylate—terminal carboxylic acid group allows conjugation to amine-containing affinity ligands using carbodiimide (EDC) and Sulfo-NHS crosslinking chemistry
Polyethylene glycol—PEG groups are flexible, non-immunogenic, hydrophilic, and significantly reduce nonspecific binding of surfaces for protein methods
Spacer arm—12-unit PEG spacer is nearly 48 angstroms long, minimizing steric hindrance for protein immobilization and binding assays

The use of MT(PEG)4 with CT(PEG)12 in surface modification can form a hydrophilic "lawn" of methyl ether-terminated PEGs with periodic exposed carboxylic acid-containing PEGs. The exposed carboxylic acid groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulfo-NHS.

Related Products
CL(PEG)12 Carboxy-PEG-Lipoamide Compound

CA(PEG)4 Carboxy-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce CA(PEG)4 is a pegylated amino acid of the form carboxy-PEG-amine, containing 4 polyethylene glycol units, and is useful for a variety of surface- and molecule-pegylation applications.

CA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with carboxy (-COOH) and amino (-NH2) termini. The unbranched, hydrophilic, discrete-length molecules have the form Carboxy-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide specific targets for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Features of CA(PEG)n Compounds

• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling or crosslinking involving primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

Applications of PEGylation
• PEGylate carboxyl or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment
• Derivatize or crosslink carboxyls or primary amines using EDC and Sulfo-NHS
• Crosslink amino terminus to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink amino terminus to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
CA(PEG)8 Carboxy-PEG-Amine Compound
CA(PEG)12 Carboxy-PEG-Amine Compound
CA(PEG)24 Carboxy-PEG-Amine Compound

MA(PEG)12 Methyl-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce MA(PEG)12 is a methyl- and amine-terminated polyethylene glycol reagent that is useful for a variety of surface-modification and molecule-pegylation applications.

Features of MA(PEG)12:

• Fully characterized PEGylation reagent with defined PEG chain length; discrete molecular weight for consistency of performance in protein-modification applications
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling of primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

MA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers and terminal methyl (-CH3) and amino (-NH2) groups. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Applications of PEGylation:
• PEGylate carboxylate or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment:
• Conjugate to carboxyl groups using EDC and Sulfo-NHS
• Crosslink to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent
• Attach to oxidized carbohydrate groups (aldehydes) by reductive amination

Why PEGylate a protein or peptide?
Methyl-capped PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in a biological application, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds:
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are a heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
MA(PEG)4 Methyl-PEG-Amine Compound
MA(PEG)8 Methyl-PEG-Amine Compound
MA(PEG)24 Methyl-PEG-Amine Compound

MM(PEG)12 Methyl-PEG-Maleimide Reagent (Thermo Scientific™)

Thermo Scientific Pierce MM(PEG)12 is a methyl-terminated, polyethylene glycol compound (12 PEG units) activated with a maleimide group for covalent pegylation of sulfhydryls on proteins (e.g., cysteines) or assay surfaces.

Features of MM(PEG)12:

• Maleimide-activated for efficient PEGylation of sulfhydryl groups at pH 6.5-7.5; reaction of maleimide group results in formation of stable, irreversible thioether bonds
• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided with 12 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome

PEGylation Applications:
• PEGylate amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length mPEG-NHS Ester Compounds:
These reagents are specially synthesized as homogeneous compounds of discrete chain length and defined molecular weight. As such, they enable precise control and optimization of surface protein modification experiments. By contrast, typical preparations of PEG compounds are heterogeneous mixtures composed of multiple chain lengths and a range of molecular weights.

Related Products
MM(PEG)24 Methyl-PEG-Maleimide Reagent

MS(PEG)8 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific™)

Thermo Scientific Pierce MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) activated as NHS esters for covalent pegylation of primary amines on proteins (e.g., lysines) or assay surfaces.

Features of MS(PEG)8:

• NHS-activated for efficient PEGylation of primary amines at pH 7-9; reaction of NHS-ester group results in formation of stable, irreversible amide bonds
• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Easy-to-follow instructions increase the likelihood of a successful outcome

MS(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with methyl (—CH3) and amine-reactive NHS-ester groups at opposite ends. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-NHS Ester, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The N-hydroxysuccinimide (NHS) ester is spontaneously reactive with primary amines (—NH2), providing for efficient PEGylation of proteins, peptides and other amine-containing molecules or surfaces.

PEGylation Applications:
• PEGylate amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length mPEG-NHS Ester Compounds
These reagents are specially synthesized as homogeneous compounds of discrete chain length and defined molecular weight. As such, they enable precise control and optimization of surface protein modification experiments. By contrast, typical preparations of PEG compounds are heterogeneous mixtures composed of multiple chain lengths and a range of molecular weights.

Related Products
MS(PEG)4 Methyl-PEG-NHS-Ester Reagent
MS(PEG)12 Methyl-PEG-NHS-Ester Reagent
MS(PEG)24 Methyl-PEG-NHS-Ester Reagent

CA(PEG)8 Carboxy-PEG-Amine Compound (Thermo Scientific™)

Thermo Scientific Pierce CA(PEG)8 is a pegylated amino acid of the form carboxy-PEG-amine, containing 8 polyethylene glycol units, and is useful for a variety of surface- and molecule-pegylation applications.

CA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers with carboxy (-COOH) and amino (-NH2) termini. The unbranched, hydrophilic, discrete-length molecules have the form Carboxy-PEGn-Amine, where the subscript "n" denotes 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide specific targets for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.

Features of CA(PEG)n Compounds

• Fully characterized PEGylation reagents with defined PEG chain lengths; molecules of discrete molecular weight for consistency of performance in protein-modification applications
• Provided as a series of 4, 8, 12 and 24 ethylene glycol units, enabling modification procedures to be optimized for a specific application while retaining all the benefits associated with protein PEGylation
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling or crosslinking involving primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcome

Applications of PEGylation
• PEGylate carboxyl or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysis

Methods of Covalent Attachment
• Derivatize or crosslink carboxyls or primary amines using EDC and Sulfo-NHS
• Crosslink amino terminus to primary amines using DSS, BS(PEG)5/BS(PEG)9 or other NHS-ester reagent
• Crosslink amino terminus to sulfhydryl groups using Sulfo-SMCC or SM(PEG)n reagent

Why PEGylate a protein or peptide?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in biological applications, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for both in vitro and in vivo applications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.

Advantages of Discrete-length Polyethylene Glycol Compounds
These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.

Related Products
CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)12 Carboxy-PEG-Amine Compound
CA(PEG)24 Carboxy-PEG-Amine Compound