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Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 30 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 20,000 daltons.
The 30 mL size is convenient for larger-scale applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 20K MWCO, 12 mL

Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 12 mL size is convenient for preparative applications and the kit format contains syringes, needles, and float buoys for convenience.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 22 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 3.5K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 3.5K MWCO, 22mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

3.5K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: Trace
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 3.5K MWCO, is composed of regenerated-cellulose dialysis tubing and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

Related Products
SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 16 mm
SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 35 mm
SnakeSkin™ Dialysis Clips

Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The 12 mL size is convenient for preparative applications
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ MINI Dialysis Device, 7K MWCO, 0.1 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >7kDa in volumes from 10 to 100 µL.

Features of Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1 mL:

Flexible format - enables user to dialyze a single sample (tube) or multiple samples (25 array)
Excellent sample recoveries - low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol - does not require laborious procedures or expensive equipment
100% leak-tested - innovative design does not permit "wicking" that can occur in home-made devices
Time savings - performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

Slide-A-Lyzer MINI Dialysis Devices allow easy sample addition and removal using a standard laboratory pipette and can be used for single or arrays of samples. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL

Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 0.5 mL size is convenient for low volume samples and gamma irradiation assures sterility.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30 mL

SnakeSkin™ Dialysis Tubing, 10K MWCO, 22 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 10K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 10K MWCO, 22mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

10K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 21%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat-width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

Related Products
SnakeSkin™ Dialysis Tubing, 10K MWCO, 16 mm
SnakeSkin™ Dialysis Tubing, 10K MWCO, 35 mm
SnakeSkin™ Dialysis Clips

SnakeSkin™ Dialysis Tubing, 10K MWCO, 16 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 10K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 10K MWCO, 16mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

10K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: 21%
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 10K MWCO, is composed of regenerated-cellulose and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat-width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

Related Products
SnakeSkin™ Dialysis Tubing, 10K MWCO, 22 mm
SnakeSkin™ Dialysis Tubing, 10K MWCO, 35 mm
SnakeSkin™ Dialysis Clips

Slide-A-Lyzer™ Dialysis Flotation Disks (Thermo Scientific™)

Handy floatation disks are for use with each Thermo Scientific Slide-A-Lyzer Dialysis Flask. When attached to the neck of the flask, the floatation disk allows the flask to float in a vertical position for simple and safe handling.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
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Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL

Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 3.5K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >3.5kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 3.5K MWCO, 0.5 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 3.5K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated to 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 3.5K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 3.5K MWCO, 2 mL

Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 3 mL size is convenient for many applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Three cassette sizes —select the cassette that best suits your sample volume
Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane

7K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 13%
Sulfur Content: 0.1 to 0.15%
Heavy Metals Content: Trace

Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL

SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 35 mm (Thermo Scientific™)

Thermo Scientific SnakeSkin Dialysis Tubing, 3.5K MWCO, is an easy and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100 mL samples.

Features of SnakeSkin Dialysis Tubing, 3.5K MWCO, 35mm:

Convenient—ready to use, pre-wetted, pleated tube
High recovery—greater than 90% protein recovery
Speed—dialysis is generally completed in 4 to 6 hours
Stability—compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

3.5K MWCO Tubing Specifications:
Membrane Type: Regenerated cellulose
Tubing Nominal Dry Thickness: 0.9 to 1.2mil (22 to 30 µM)
Glycerol Content: Trace
Sulfur Content: 0.1% to 0.15%
Heavy Metals Content: Trace

SnakeSkin Dialysis Tubing, 3.5K MWCO, is composed of regenerated-cellulose dialysis tubing and supplied as an open, pleated (telescoped) tube. SnakeSkin Dialysis Tubing is supplied in eight inch (20 cm) sticks containing 35 feet of dialysis tubing having a 16, 22, or 35mm circular internal diameter (I.D.). With similar capacity to 10.5 meters of 23 to 50 diameter dry flat width tubing, the hydrated SnakeSkin Dialysis Tubing holds ~2 to 10 mL of sample per centimeter of length.

The easy-open, pleated format of SnakeSkin Dialysis Tubing was developed to simplify large volume sample dialysis. To use, simply pull out and cut off the required length of tubing, fold over one end of tubing and apply a dialysis clip to close it, add sample at the open end and use a second clip to close the remaining end. Clips are sold separately. In comparison, many traditional dialysis tubing membranes that are supplied as dry, flattened rolls that are difficult to open and often require extensive soaking before they can be used.

The SnakeSkin membrane-pleating process does not change the tubing's MWCO. Also, any low MW contaminants present are removed during the dialysis process. Because SnakeSkin Dialysis Tubing is made from the same type of regenerated cellulose as flat tubing, its dialysis performance matches that of conventional tubing.

More Product Data
Separation characteristics of dialysis membranes

Related Products
SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 22 mm
SnakeSkin™ Dialysis Tubing, 3.5K MWCO, 16 mm
SnakeSkin™ Dialysis Clips

Competition Rapid Equilibrium Dialysis (RED) Inserts (Thermo Scientific™)

Thermo Scientific Pierce Competition RED Device Inserts and Base Plates comprise a specially designed system for evaluating competitive binding interactions using rapid equilibrium dialysis (RED).

The Competition RED Device was developed in association with pharmaceutical laboratories to more accurately model in vivo drug interactions by enabling equilibrium dialysis experiments to be performed in a multiplexed format involving competition for binding among different tissues. The Competition RED Device System consists of disposable dialysis tube inserts and a reusable Base Plate made of high-grade PTFE. The Base Plate is much like our regular RED Device Plate except that it is divided into different size chambers (wells) for positioning 2-8 RED Device Inserts per well. The format enables competitive dialysis experiments involving 2-15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes a selection of both types). The Competition RED Device requires no extensive assembly or specialized equipment, and each chamber/well is easily accessible from the top of the device.

Features of the Competition Rapid Equilibrium Dialysis (RED) Device:

Easy to use—disposable tubes require no presoaking step, assembly, or specialized equipment
Short incubation time—design provides high surface-to-volume ratio of membrane to sample, enabling equilibrium to be reached within 2-4 hours
Flexible format—base plate contains several different chamber sizes, enabling small molecule partitioning studies involving 2-16 tissue or protein samples without waste
Robust—compartmentalized design eliminates potential for cross talk or leakage
Reproducible and accurate—perform controlled experiments with multiple tissues to obtain screening results that have high predictability for in vivo studies with animal models
Validated quality—base plate is composed of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination; each lot is functionally tested in a protein binding assay for guaranteed performance

Applications:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Aids in determining formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

Determining the extent to which a molecule partitions between plasma and specific tissues in the body is a critical phase of drug development because it determines compound dosing, efficacy, clearance rate, and the potential for drug interactions or tissue damage. Although final assessment of these variables invariably requires animal dosing and analysis, in vitro screening systems that are rapid and have good predictability are highly desirable for ADME-Tox studies. Equilibrium dialysis is among the most successful of in vitro screening method for evaluating drug binding and dosage requirements. Equilibrium dialysis is used to estimate the non-bound drug fraction in plasma or drug partitioning between red blood cell and plasma, plasma and liver microsomes, or plasma and tissue homogenates. However, many tissue-partitioning studies indicate that certain drugs show significant binding to tissues tested individually but have considerably different binding preferences when placed in competition between multiple tissues. The Competition RED Device extends the power of equilibrium dialysis by facilitating analysis of simultaneous drug interactions and partitioning among multiple tissues.

Posters:
• (Xia, C. et al.)
• (Qian, M.G., et al.)
• (Matuszak, K. and Custer, C.)

Related Products
Competition Rapid Equilibrium Dialysis (RED) Base Plate

Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 15 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO), gamma-irradiated:

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Gamma-irradiated—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 70 mL

Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (2K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 2,000 daltons.
The 3 mL size is convenient for many applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO, 30 mL