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Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 12 mL size is convenient for preparative applications and the kit format contains syringes, needles, and float buoys for convenience.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The 12 mL size is convenient for preparative applications
Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassette Kit, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 30 mL

Slide-A-Lyzer™ MINI Dialysis Device, 7K MWCO, 0.1 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 7K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >7kDa in volumes from 10 to 100 µL.

Features of Slide-A-Lyzer MINI Dialysis Device, 7K MWCO, 0.1 mL:

Flexible format - enables user to dialyze a single sample (tube) or multiple samples (25 array)
Excellent sample recoveries - low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol - does not require laborious procedures or expensive equipment
100% leak-tested - innovative design does not permit "wicking" that can occur in home-made devices
Time savings - performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

Slide-A-Lyzer MINI Dialysis Devices allow easy sample addition and removal using a standard laboratory pipette and can be used for single or arrays of samples. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL

Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 250 mL.

Features of Slide-A-Lyzer Dialysis Flasks, 2K MWCO, 250 mL:

Easy to use – Simply pipette or pour sample into flask and begin dialysis
Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
Multiple molecular-weight cut-offs – select the membrane MWCO that best suits your sample's molecular weight
Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color

Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace

Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flotation Disks

Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.1 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 10K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >10kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 10K MWCO, 0.1 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 10K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

Related Products
Float Buoys for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device Kit, 10K MWCO, 0.1 mL
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ MINI Dialysis Device, 10K MWCO, 2 mL

Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications and gamma irradiation assures sterility.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 30 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO), gamma-irradiated:

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
Gamma-irradiated—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes

10K Dialysis Membrane Specifications
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL.

Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 10K MWCO, 30 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 0.5 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 3 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 15 mL
Slide-A-Lyzer™ G2 Dialysis Cassettes, gamma-irradiated, 10K MWCO, 70 mL

Slide-A-Lyzer™ Dialysis Flasks, 3.5K MWCO, 230 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Flasks, 3.5K MWCO, 230 mL facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules in sample volumes up to 230 mL.

Features of Slide-A-Lyzer Dialysis Flasks, 3.5K MWCO, 230 mL:

Easy to use – Simply pipette or pour sample into flask and begin dialysis
Fast dialysis – flat flask chamber with two membranes provides high surface-area to volume ratio, enabling dialysis of a 230 mL sample in 8 hours to overnight
High recovery – rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
Multiple molecular-weight cuto-ffs – select the membrane MWCO that best suits your sample's molecular weight
Color-coded frames – easily identify membrane pore size (MWCO) based on the frame color

Dialysis Membrane Specifications:
• Composition: Regenerated-cellulose
• Thickness: 0.9 to 1.2mil (22.5 to 30μm); 2K MWCO = 2mil (50μm)
• Hydration Required Before Use: 30 seconds to 2 minutes
• Glycerol Content: Trace (10K MWCO content is 21%)
• Sulfur Content: 0.04 to 0.17%
• Heavy metals Content: Trace

Slide-A-Lyzer Dialysis Flasks are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units. Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to ensure maximum sample recovery and sample purity. The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: Purple (20,000 daltons), Orange (10,000 daltons), Pink (3500 daltons), and Blue (2000 daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished in 8 hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation and temperature. Minimum dialysis time must be determined empirically for each specific system.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 2K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flasks, 20K MWCO, 250 mL
Slide-A-Lyzer™ Dialysis Flotation Disks

Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 12 mL size is convenient for many preparative applications and the kit format contains syringes, needles, and float buoys for convenience.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Three cassette sizes —select the cassette that best suits your sample volume
Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane

7K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 13%
Sulfur Content: 0.1 to 0.15%
Heavy Metals Content: Trace

Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 12 mL

Slide-A-Lyzer™ G2 Dialysis Cassettes, 7K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (7K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (7K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—two cassette capacities to optimally match 0.25 to 3 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 7K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 7000 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. The membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ G2 Dialysis Cassettes, 7K MWCO, 3 mL

Slide-A-Lyzer™ MINI Dialysis Device, 20K MWCO, 0.1 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices, 20K MWCO are disposable polypropylene cups with integrated, low-binding membranes for dialysis and high recovery of proteins and macromolecules >20kDa in volumes from 10 µL to 2 mL.

Features of Slide-A-Lyzer MINI Dialysis Device, 20K MWCO, 2 mL:

Three convenient sizes – dialysis devices for 0.1, 0.5 and 2 mL samples fit standard 1.5 mL-micro centrifuge, 15 mL- and 50 mL-conical tubes, respectively
Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems
One-step protocol – pipette sample into the MINI Device and place in tube containing the dialysis buffer; no laborious assembly, device preparation or expensive equipment are required
100% leak-tested – innovative design does not permit "wicking" that can occur in home-made devices
Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

These 20K MWCO dialysis devices are available in three sizes (0.1, 0.5 and 2 mL capacities), and are designed to allow easy sample addition and removal using a standard laboratory pipette. The MINI Devices are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices were developed as a convenient means for dialysing small-volume samples of nucleic acids and proteins without undue sample loss. Low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, micro conjugation reactions, and concentration can be accomplished with these devices. Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filter/clean environment and auto-bagged after assembly to ensure that they are free of contaminants and ready to use.

The 0.5 mL and 2 mL MINI Dialysis Devices fit in and are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of spills or contamination, and eliminates the need for floats or large beakers of buffer. Using these MINI Dialysis Devices, low molecular weight contaminant removal, buffer exchange and desalting, can be accomplished within 4 to 8 hours with efficiencies, rates, and recoveries very similar to conventional dialysis using a large dialysate volume (view data).

The 0.1 mL MINI Dialysis Devices are designed to be capped (caps included) and inserted into standard 1.5 mL micro centrifuge tubes. Alternatively, as many as 25 MINI Devices can be placed in a Slide-A-Lyzer MINI Dialysis Device Float for dialysis of multiple inside a large beaker. Using the 0.1 mL MINI Device, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in less than 10 minutes.

As with all dialysis applications, the observed rate of dialysis for each sample used with these devices depends on a variety of factors including membrane to sample volume, temperature and agitation rates; as well as the molecular weight, shape, degree of hydration, and charge of the molecules passing through the membranes and the pH, ionic strength, and solvent characteristic of the buffers being used.

More Product Data
New dimensions for low-volume sample dialysis
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, gamma-irradiated, 10K MWCO, 12 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 12 mL size is convenient for preparative applications and gamma irradiation assures sterility.

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 12 mL
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Slide-A-Lyzer™ Dialysis Cassettes, 7K MWCO, 0.5 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (7K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 7000 daltons.
The 0.5 mL size is convenient for low volume samples

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis —flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Three cassette sizes —select the cassette that best suits your sample volume
Color-coded —green cassette frame specifies cassettes with the 7000 MW pore size membrane

7K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 13%
Sulfur Content: 0.1 to 0.15%
Heavy Metals Content: Trace

Available in three different cassette sizes to accommodate sample volumes between 0.1 and 12 mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

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Slide-A-Lyzer™ Dialysis Cassette Kit, 7K MWCO, 12 mL

Slide-A-Lyzer™ Dialysis Cassettes, 10K MWCO, 3 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
The 3 mL size is convenient for many applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
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Slide-A-Lyzer™ G2 Dialysis Cassettes, 3.5K MWCO, 15 mL (Thermo Scientific™)

Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (3.5K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.

Features of Slide-A-Lyzer G2 Dialysis Cassettes (3.5K MWCO):

Easy-loading—pipette-accessible for easy sample loading and retrieval
Self-floating—integrated air chambers eliminate the need for float buoys
Sturdy construction—ensures the highest possible sample integrity and protection
Superior design—thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes—five cassette capacities to optimally match 0.25 to 70 mL sample volumes
Versatile—ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting

The 3.5K molecular-weight cut off (MWCO) cassettes use a cellulose membrane that is rated to completely retain proteins and other macromolecules that are larger than 3500 daltons while allowing removal of buffer salts and small contaminants. The cassette membrane is composed of low-binding regenerated cellulose. The membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free.

The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70 mL. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.

More Product Data
Separation characteristics of dialysis membranes

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