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Vacuum Hydrolysis Tube, 1 mL Thermo Scientific™

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 1 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 1 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 6 mL
Vacuum Hydrolysis Tube, 18 mL

Vacuum Hydrolysis Tube, 6 mL Thermo Scientific™

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 6 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 6 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 18 mL

Samco™ 16 × 125mm Disposable Culture Tubes (DCTs) Thermo Scientific™

Choose the material that best suits your needs with Thermo Scientific™ Samco™ 16 × 125mm Disposable culture tubes, available in clarified polypropylene for strength and resistance, or polystyrene for superior clarity. Designed for most laboratory applications, these plastic tubes are chemical free and biologically inert. Each tube is precision molded and manufactured from premium grade materials. Our processes are monitored with quality checks which provide continued lot-to-lot uniformity and reliable performance. 

Sterilin™ Borosilicate Glass Culture Tubes Thermo Scientific™

Rely on chemical and temperature resistant borosilicate glass culture tubes in your tough applications. Thermo Scientific™ Sterilin™ Borosilicate Glass Culture Tubes are available with flat or round bottom, threaded to accept a screw cap.
NOT AVAILABLE IN AMERICA

Nunc™ Cell Culture Tubes Thermo Scientific™

Perform adherent cell cultures in biotechnology, pharmaceutical and research laboratories, as well as in the production of vaccines and diagnostic kits with Thermo Scientific™ Nunc™ Cell Culture Tubes.

Vacuum Hydrolysis Tube, 18 mL Thermo Scientific™

Thermo Scientific Pierce Vacuum Hydrolysis Tube, 18 mL is a high-quality glass tube designed for heated, acid-based, vacuum hydrolysis of proteins and peptides for amino acid analysis.

Features of Vacuum Hydrolysis Tube, 18 mL:

• Saves 10-18 hours on hydrolysis procedures when combined with 6N HCl (Part No. 24308)
• Provides for hydrolysis in 6 hours when heated at 150°C in a dry block heater (Note: the upper temperature limit of Vacuum Hydrolysis Tubes is 260°C in a block heater but only 110°C in an oven)
• Optimal hydrolysis control when used with Reacti-Therm Dry Block Heating System
• Compatible-fitting are available for use with Thermo Scientific Reacti-Therm Heating Modules

Applications of Vacuum Hydrolysis Tube:
• Protein/peptide hydrolysis
• Sample concentration
• Lyophilization
• Hydrazinolysis

Vacuum Hydrolysis Tubes are precision-designed for use in protein hydrolysis and subsequent analysis of protein and peptide amino acid compositions. The heavy-walled borosilicate glass construction ensures complete compatibility with all commonly used acid hydrolysis reagents. Acid hydrolysis at elevated temperatures, in vacuo, will hydrolyze most proteins and peptides to their amino acid components without significant decomposition.

Vacuum hydrolysis methods commonly use 6N constant boiling hydrochloric acid (HCl), a mixture of HCl and propionic acid, 3N mercaptoethanesulfonic acid or 4N methanesulfonic acid. The constant boiling HCl method uses standard protein hydrolysis at 105 to 110°C for 16 to 24 hours. Using the HCl/propionic acid method, hydrolysis times can be shortened to 15 minutes at 160°C. Tryptophan is easily degraded by acid hydrolysis in 6N HCl. The presence of oxygen, heavy metals and carbohydrates are contributing factors to this degradation. A 22-hour hydrolysis method was developed to improve tryptophan recovery from protein hydrolysis samples by using 3N mercaptoethanesulfonic acid. A second method to improve tryptophan recovery involves hydrolysis at 115°C using 4N methanesulfonic acid.

Related Products
Vacuum Hydrolysis Tube, 1 mL
Vacuum Hydrolysis Tube, 6 mL

Samco™ 17 × 100mm Disposable Culture Tubes (DCTs) Thermo Scientific™

Perform most lab applications with Thermo Scientific™ Samco™ 17 × 100mm Disposable Culture Tubes, which deliver lot-to-lot uniformity and reliable performance. These chemical-free and biologically inert tubes are offered in clarified polypropylene for strength and resistance, or polystyrene for superior clarity. Each tube is precision molded and manufactured from premium grade materials by processes monitored with quality checks.

Immuno Tubes and Stoppers Thermo Scientific™

Reproduce results time after time with Thermo Scientific™ Immuno Tubes and Stoppers, ideal for ELISA, solid phase RIA and liquid phase RIA assays. These products are backed by industry-leading quality standards and certifications.

Samco™ 13 × 100mm Disposable Culture Tubes (DCTs) Thermo Scientific™

Choose the material that best suits your needs with Thermo Scientific™ Samco™ 13 × 100mm Disposable Culture Tubes, available in clarified polypropylene for strength and resistance, or polystyrene for superior clarity. Designed for most laboratory applications, these DCTs are chemical free and biologically inert. Each tube is precision molded and manufactured from premium grade materials. Our processes are monitored with quality checks which provide continued lot-to-lot uniformity and reliable performance. All sterile tubes are gamma-irradiated and ready to use.

Samco™ 12 × 75mm Disposable Culture Tubes (DCTs) Thermo Scientific™

Perform most laboratory applications with chemical-free, biologically inert polystyrene Thermo Scientific™ Samco™ 12 × 75mm Disposable Culture Tubes, which feature superior clarity. Each tube is precision molded and manufactured from premium grade materials. Our processes are monitored with quality checks which provide continued lot-to-lot uniformity and reliable performance. All sterile tubes are gamma-irradiated and ready to use.

Low Protein Binding Collection Tubes (1.5 mL) Thermo Scientific™

Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL) help minimize protein sample loss during sample manipulation and are ideal in applications where processing steps are extensive.

Durable—able to tolerate centrifuge speeds up to 20,000 RCF
Compatible—suitable for IP and mass spectrometry applications
Nuclease-free—certified RNase/DNase-free

The Pierce Low Protein Binding Microcentrifuge Tubes help minimize sample loss in mass spectrometry and immunoprecipitation/co-immunoprecipitation applications. The polypropylene tubes are certified as DNase/RNase-free and are conveniently packaged (250 tubes per package) to minimize contamination.

Covaris™ M220 miniTUBE, Red Ion Torrent™

The Covaris® M220 Red miniTUBE is specifically engineered to shear genomic DNA into fragment size distributions centered on 5 kb. Fragment distributions are tight and precisely centered on the targeted size. Each red miniTUBE can process 200 µl of sample. Each package contains 25 tubes.

Phasemaker™ Tubes Invitrogen™

Invitrogen™ Phasemaker™ Tubes contain a thick liquid polymer that separates and completely isolates the upper aqueous phase of a TRIzol® Reagent mix from the organic phase underneath, greatly simplifying removal of the upper phase containing the RNA.

• Pre-dispensed, 2 mL tubes
• Exclusively for RNA extraction
• For use with TRIzol Reagent, TRIzol® LS Reagent, and TRIzol® Plus Purification Kit only
• Simplifies removal of aqueous phase
• Helps increase RNA yield up to 30%
• Does not interfere with downstream experiments

The polymer in Phasemaker Tubes is heavier than the aqueous phase of the TRIzol Reagent mix, but lighter than the organic phase, so after centrifugation it positions itself between these two layers. As a thick polymer, it creates a tight seal between the two phases, easing removal of the aqueous phase and helping to reduce the risk of carrying over contaminants from the organic layer, thereby increasing RNA recovery up to 30%.

The polymer in the Phasemaker Tubes is inert and does not interfere with standard RNA applications. Isolated RNA can be used in RT-PCR, Northern blot analysis, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.

Phasemaker Tubes accommodate RNA extraction using TRIzol Reagent, TRIzol LS Reagent, and TRIzol Plus Purification Kit only. They are not recommended for use with other organic reagents as the density of those reagents might not be suitable. Phasemaker Tubes are not for use in DNA or protein isolation, but are intended for RNA extraction only.

Phasemaker Tubes work with fresh or frozen samples, including tissue and blood. They also work with samples previously treated with RNAlater™ RNA stabilization solution. Phasemaker Tubes are already pre-dispensed; the tube size is 2 mL.

Genetic Analyzer Sample Tubes, 0.5 mL Applied Biosystems™

These are sample tubes for use in the 48-sample tray of the 310 Genetic Analyzer.

Use with: 1.0 mL Glass Syringe, 310 Genetic Analyzer Capillary (47 cm, 36 cm wtr), 310 Running Buffer (10X), BigDye® Terminator v1.1 Cycle Sequencing Kit, GeneScan™ 500 ROX™ Size Standard, Hi-Di™ Formamide, MicroAmp® Reaction Tube with Cap (0.2 ml), POP-4™ Polymer for the 310 Genetic Analyzer, POP-6™ Polymer for the 310 Genetic Analyzer, Septa for 0.5 ml Sample Tubes

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Sterilin™ Round Base Rimless Borosilicate Glass Rimless Tubes Thermo Scientific™

Trust these round-base borosilicate glass tubes in high temperature, aggressive chemical applications. Thermo Scientific™ Sterilin™ Round Base Rimless Borosilicate Glass Tubes are ideal for use with samples that are sensitive to plastic tube leachables.
NOT AVAILABLE IN AMERICA
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