Shop All Immunoassay Microplates

Plates and Modules with Affinity Binding Surfaces (Thermo Scientific™)

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Immuno Clear Standard Modules (Thermo Scientific™)

Perform applications which are delivering well-to-well reproducibility with Immuno Clear Standard Modules. Manufactured to fit standard equipment.

Nunc™ NucleoLink™ Strips (Thermo Scientific™)

Ideal for hybridization assays due to the heat resistant resin, the unique breakable module strip design offers error-free flexibility, accurate reading and efficient washing.

Pierce™ Maleimide Activated Plates, Black, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated Black, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated Black, 96-Well Plates:

• Black, for fluorescent assays
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

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Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip
Pierce™ Maleimide Activated Plates, White, 96-Well

White 96-Well Immuno Plates (Thermo Scientific™)

Conduct quantitative and qualitative immunoassays like ELISA and binding assays with Thermo Scientific™ White 96-Well Immuno Plates

Clear Round-Bottom Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Carry out colormetric assays with reliable and reproducible results using these clear round-bottom immuno nonsterile 96-well plates, offered with a choice of surface.

ProcartaPlex™ 96 well Flat Bottom Plate, black (Invitrogen™)

The black Greiner 96 well flat bottom plate is ideal for use with all ProcartaPlex Luminex Assys.The plate is included in all ProcartaPlex Multiplex and Basic Kits.

*If you would like to place a bulk order please contact your sales representative.

Reactivity/Species
Canine, Human, Mouse, Non-human primate, Pig, Rat

Reported Application
Multiplex Immunoassay

Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Black,384-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Black, 384-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well

Black 96-Well Immuno Plates (Thermo Scientific™)

Perform quantitative and qualitative immunoassays like ELISA and binding assays with a choice of three surface types.

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 8-Well Strip Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well

Strip Assembly, 1 x 12, EB (Thermo Scientific™)

Perform applications which are delivering well-to-well reproducibility with Immuno Clear Standard Modules. Manufactured to fit standard equipment.

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are black for fluorescent assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well

Pierce™ Protein A Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

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Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A Coated Plates, Black, 96-Well

Pierce™ Glutathione Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

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Pierce™ Glutathione Coated Plates, Clear, 8-Well Strip
Pierce™ Glutathione Coated Plates, Black, 96-Well

Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Mouse IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are black for fluorescent assays.

Features of Goat Anti-Mouse IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~7 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

Related Products
Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well