Shop All Immunoassay Microplates

Plates and Modules with Affinity Binding Surfaces (Thermo Scientific™)

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Immuno Clear Standard Modules (Thermo Scientific™)

Perform applications which are delivering well-to-well reproducibility with Immuno Clear Standard Modules. Manufactured to fit standard equipment.

Nunc™ NucleoLink™ Strips (Thermo Scientific™)

Ideal for hybridization assays due to the heat resistant resin, the unique breakable module strip design offers error-free flexibility, accurate reading and efficient washing.

Pierce™ Maleimide Activated Plates, Black, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated Black, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated Black, 96-Well Plates:

• Black, for fluorescent assays
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

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Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip
Pierce™ Maleimide Activated Plates, White, 96-Well

White 96-Well Immuno Plates (Thermo Scientific™)

Conduct quantitative and qualitative immunoassays like ELISA and binding assays with Thermo Scientific™ White 96-Well Immuno Plates

Clear Round-Bottom Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Carry out colormetric assays with reliable and reproducible results using these clear round-bottom immuno nonsterile 96-well plates, offered with a choice of surface.

ProcartaPlex™ 96 well Flat Bottom Plate, black (Invitrogen™)

The black Greiner 96 well flat bottom plate is ideal for use with all ProcartaPlex Luminex Assys.The plate is included in all ProcartaPlex Multiplex and Basic Kits.

*If you would like to place a bulk order please contact your sales representative.

Reactivity/Species
Canine, Human, Mouse, Non-human primate, Pig, Rat

Reported Application
Multiplex Immunoassay

Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Black,384-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Black, 384-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well

Black 96-Well Immuno Plates (Thermo Scientific™)

Perform quantitative and qualitative immunoassays like ELISA and binding assays with a choice of three surface types.

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 8-Well Strip Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well

Strip Assembly, 1 x 12, EB (Thermo Scientific™)

Perform applications which are delivering well-to-well reproducibility with Immuno Clear Standard Modules. Manufactured to fit standard equipment.

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are black for fluorescent assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well

Pierce™ Protein A Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

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Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A Coated Plates, Black, 96-Well

Pierce™ Glutathione Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

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Pierce™ Glutathione Coated Plates, Clear, 8-Well Strip
Pierce™ Glutathione Coated Plates, Black, 96-Well

Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Mouse IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are black for fluorescent assays.

Features of Goat Anti-Mouse IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~7 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well

White and Black 384-Well Immuno Plates (Thermo Scientific™)

Conduct quantitative and qualitative immunoassays with Thermo Scientific™ White and Black 384-Well Immuno Plates.

Plates and Modules with Covalent Binding Surfaces (Thermo Scientific™)

Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces

Pierce™ Biotin Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Biotin Coated Plates can be used in any immunoassay with Thermo Scientific NeutrAvidin Biotin-Binding Protein, streptavidin, avidin, or other biotin-binding proteins.

Features of Biotin Coated Plates:

• Biotin group accessible for binding avidin or streptavidin
• Pre-blocked to reduce nonspecific binding
• Strip well plate format for the ultimate in convenience
• Coat volume: 200 µL
• Blocking volume: 300 µL

Biotin coated plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with streptavidin, avidin, or other biotin-binding proteins.

Immuno Breakable Modules Clear (Thermo Scientific™)

Choose from four surfaces and a variety of wells and configurations. Immuno Breakable Modules offer an optimal choice of products for the specific assay.

Clear C-Shaped Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Perform quantitative and qualitative solid phase immunoassays with these nonsterile 96-well plates, available in a variety of binding surfaces.

Pierce™ Streptavidin Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 96-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Clear, 96-Well:

• Approximate binding capacity is ~10 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip
Pierce™ Streptavidin Coated Plates, Clear, 384-Well

Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 8-Well Strip are pre-blocked and ready-to-use polystyrene for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Clear, 8-Well Strip:

• Approximate binding capacity is ~10 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear, 8-Well Strip are pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip (with SuperBlock™ Buffer)
Pierce™ Streptavidin Coated Plates, Clear, 96-Well
Pierce™ Streptavidin Coated Plates, Clear, 384-Well

Nunc MaxiSorp™ flat-bottom (Invitrogen™)

The Nunc MaxiSorp™ high protein-binding capacity 96 well ELISA plates are recommended for use in capturing immunoglobulins found in eBioscience Ready-SET-Go! ELISA kits, as well as other matched antibody pairs for ELISA. Maxisorp plates have a protein-binding capacity of approximately 600-650 ng IgG/cm2.

Reported Application
ELISA

Pierce™ Streptavidin Coated Plates, Clear, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 384-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Clear, 384-Well:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

Related Products
Pierce™ Streptavidin Coated Plates, Clear, 96-Well
Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip

Clear Flat-Bottom Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Choose from four binding surfaces for reliable and reproducible results in colormetric assays.

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are clear for colorimetric assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

Related Products
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well

Immuno Breakable Modules Clear (Thermo Scientific™)

Choose from four surfaces and a variety of wells and configurations. Immuno Breakable Modules offer an optimal choice of products for the specific assay.

Pierce™ Maleic Anhydride Activated Plates, Clear, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleic Anhydride Activated 96-Well Plates are clear, 96-well plates that allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Features of Maleic Anhydride Activated Plates:

• Spontaneously react with primary amines
• A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

Related Products
Pierce™ Maleic Anhydride Activated Plates, Clear, 8-Well Strip
Pierce™ Maleic Anhydride Activated Plates, White, 96-Well

Pierce™ Protein A Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, White, 96-Well
Pierce™ Protein A Coated Plates, Black, 96-Well

Pierce™ Copper Coated High Capacity Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

These copper coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. Pierce Copper Coated Plates have high binding capacity because they are coating using an exclusive process that increases the amount of histidine-tagged protein that will bind to the plate surface. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range. The copper-coated plates are available as clear, white or black plates that can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Features of Copper Coated High Binding Capacity Plates:

• Quantitate previously undetectable histidine-tagged proteins
• Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
• Copper chelate provides greater binding capacity than nickel
• The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
• Pre-blocked for the ultimate convenience

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.

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Pierce™ Copper Coated High Capacity Plates, Clear, 96-Well
Pierce™ Copper Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ Copper Coated High Capacity Plates, White, 96-Well