Shop All Immunoassay Microplates

Immuno Clear Standard Modules (Thermo Scientific™)

Perform applications which are delivering well-to-well reproducibility with Immuno Clear Standard Modules. Manufactured to fit standard equipment.

Microlite™ White Microtiter™ Plates (Thermo Scientific™)

Experience consistent binding, high reflectivity, and low crosstalk for use in luminescent immunoassays and related techniques using Microlite Microtiter Plates.

Clear C-Shaped Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Perform quantitative and qualitative solid phase immunoassays with these nonsterile 96-well plates, available in a variety of binding surfaces.

ProcartaPlex™ 96 well Filter Plate (Invitrogen™)

The 96 well Filter Plate is ideal for use with a vacuum system.

*Manuals for use for ProcartaPlex products are available upon request. Please contact LuminexFAS@thermofisher.com for more information.

Reactivity/Species
Canine, Human, Mouse, Non-human primate, Pig, Rat

Reported Application
Multiplex Immunoassay

ProcartaPlex™ 384 well Filter Plate (Invitrogen™)

The 384 well Filter Plate is ideal for use with a vacuum system.

Manuals for use for ProcartaPlex products are available upon request. Please contact LuminexFAS@thermofisher.com for more information.

Reactivity/Species
Canine, Human, Mouse, Non-human primate, Pig, Rat

Reported Application
Multiplex Immunoassay

White 96-Well Immuno Plates (Thermo Scientific™)

Conduct quantitative and qualitative immunoassays like ELISA and binding assays with Thermo Scientific™ White 96-Well Immuno Plates

Clear Flat-Bottom Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Choose from four binding surfaces for reliable and reproducible results in colormetric assays.

ProcartaPlex™ 96 well Flat Bottom Plate, black (Invitrogen™)

The black Greiner 96 well flat bottom plate is ideal for use with all ProcartaPlex Luminex Assys.The plate is included in all ProcartaPlex Multiplex and Basic Kits.

*If you would like to place a bulk order please contact your sales representative.

Reactivity/Species
Canine, Human, Mouse, Non-human primate, Pig, Rat

Reported Application
Multiplex Immunoassay

Black 96-Well Immuno Plates (Thermo Scientific™)

Perform quantitative and qualitative immunoassays like ELISA and binding assays with a choice of three surface types.

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Black 96-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 384-Well

Pierce™ Protein A/G Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Clear, Protein A/G Coated 8-Well Strip Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein A/G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein A/G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein A/G Coated Microplates:
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Related Products
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein L Coated Plates, Clear, 96-Well

Pierce™ Protein A Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A Coated Plates, White, 96-Well

Nunc™ TopYield™ Strips (Thermo Scientific™)

Designed for liquid phase PCR or Immuno-PCR, these microplate strips offer error-free flexibility, well lock into frame for accurate reading and efficient washing.

Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 8-Well Strip are pre-blocked and ready-to-use polystyrene for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Clear, 8-Well Strip:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear, 8-Well Strip are pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

Related Products
Pierce™ Streptavidin Coated Plates, Clear, 96-Well
Pierce™ Streptavidin Coated Plates, Clear, 384-Well

Immuno Breakable Modules in White and Black (Thermo Scientific™)

Keep wells in place with Thermo Scientific™ Breakable Modules in White and Black, ensuring fluorescence and luminescence assays are easy to handle.

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 96-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well

Pierce™ Maleimide Activated Plates, White, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated White, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated White, 96-Well Plates:

• White, for chemiluminescent assays
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

Related Products
Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip
Pierce™ Maleimide Activated Plates, Black, 96-Well

Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity White 96-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 384-Well

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 384-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip

Clear Flat-Bottom Immuno Nonsterile 384-Well Plates (Thermo Scientific™)

Carry out colormetric assays with reliable and reproducible results using these clear flat-bottom immuno nonsterile 384-well plates, offered with a choice of surface.

Immuno Breakable Modules Clear (Thermo Scientific™)

Choose from four surfaces and a variety of wells and configurations. Immuno Breakable Modules offer an optimal choice of products for the specific assay.

Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, White, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, White, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well

Pierce™ Protein G Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce White, Protein G Coated 96-Well Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce™ Protein G Coated Plates, Clear, 96-Well
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Black, 96-Well

Pierce™ Streptavidin Coated Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Black, 384-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Black, 384-Well:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Black are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

Related Products
Pierce™ Streptavidin Coated Plates, White, 96-Well
Pierce™ Streptavidin Coated Plates, Black, 96-Well

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are white for chemiluminescent assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

Related Products
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well

QuantiGene™ Plex Magnetic Separation Plates (Invitrogen™)

QuantiGene Magnetic Separation Plates are 96-well flat-bottom plates used for the branched DNA and SAPE incubations on the second day of the QuantiGene Plex Assay. The Magnetic Separation Plates are compatible with the QuantiGene Handheld Magnetic Plate Washer (Cat. No. QP0702) and QuantiGene Plex Plate Seals (Cat. No. QG0524). These plates are one component of several included in the QuantiGene Plex Assay kit, and are only intended to supplement the assay kit when more plates are needed.

Pierce™ Protein L Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein L, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein L Coated Microplates:
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Related Products
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A/G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip

Pierce™ Nickel Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated Black 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These black plates can be used with fluorescent detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Related Products
Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well

Pierce™ Streptavidin Coated High Capacity Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Clear, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Clear, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ Streptavidin Coated High Capacity Plates, Clear, 384-Well

Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Black, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Black, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well

Pierce™ Protein G Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Clear, Protein G Coated 8-Well Strip Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Related Products
Pierce™ Protein G Coated Plates, Clear, 96-Well
Pierce™ Protein G Coated Plates, White, 96-Well
Pierce™ Protein G Coated Plates, Black, 96-Well

Immuno Standard Modules White and Black (Thermo Scientific™)

Conduct fluorescence- and luminescence-based immunoassays and binding assays using Thermo Scientific™ Immuno Standard Modules White and Black.

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Black 384-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 384-Well

Plates and Modules with Affinity Binding Surfaces (Thermo Scientific™)

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Pierce™ NeutrAvidin™ Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Clear 8-Well Strip Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Clear 8-Well Strip Plates:

• Clear plates for colorimetric detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

Related Products
Pierce™ NeutrAvidin™ Coated Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated Plates, Clear, 384-Well

Pierce™ Maleimide Activated Plates, Black, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated Black, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated Black, 96-Well Plates:

• Black, for fluorescent assays
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

Related Products
Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip
Pierce™ Maleimide Activated Plates, White, 96-Well

Pierce™ Streptavidin Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 96-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Clear, 96-Well:

• Approximate binding capacity is ~10 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

Related Products
Pierce™ Streptavidin Coated Plates, Clear, 8-Well Strip
Pierce™ Streptavidin Coated Plates, Clear, 384-Well

Pierce™ Glutathione Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

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Pierce™ NeutrAvidin™ Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Clear 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Clear 96-Well Plates:

• Clear plates for colorimetric detection methods
• Pre-blocked with Blocker BSA Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, Clear, 8-Well Strip
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Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated White 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated White 96-Well Plates:

• White plates for chemiluminescent detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well
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Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well

Pierce™ Maleic Anhydride Activated Plates, Clear, 8-Well Strip (Thermo Scientific™)

These Thermo Scientific Pierce Maleic Anhydride Activated 8-Well Strip Plates are clear, 96-well strip plates that allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Features of Maleic Anhydride Activated Plates:

• Spontaneously react with primary amines
• A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

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Pierce™ Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.

Features of Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip

Sensitive—detect down to 5 ng/mL of biotinylated IgG
Specific—low background with high signal-to-noise ratios
Versatile—use either fluorescence, chemiluminescence or colorimetric detection
Robust—broad dynamic range
Ready to use—plates are supplied pre-blocked to save time

Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low non-specific interactions.

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Pierce™ Nickel Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated Clear 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These clear plates can be used with colorimetric detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

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Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Black 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Black 96-Well Plates:

• Black plates for fluorescent detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well
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Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well

Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated White 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated White 96-Well Plates:

• White plates for chemiluminescent detection methods
• Pre-blocked with Blocker BSA Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Immuno Clear Standard Modules, Framed (Thermo Scientific™)

Immuno Clear Standard Modules feature Nunc™, Immulon™ and Microtiter™ module plates that are developed for ELISA techniques and fit standard equipment.

Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated Clear, 8-Well Strip Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated Clear, 8-Well Strip Plates:

• Clear, for colorimetric assays
• Convenient 8-well strip format
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

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Pierce™ NeutrAvidin™ Coated Plates, Clear, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Clear 384-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Clear 384-Well Plates:

• Clear plates for colorimetric detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ Protein G Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Clear 96-Well Protein G Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

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Clear Round-Bottom Immuno Nonsterile 96-Well Plates (Thermo Scientific™)

Carry out colormetric assays with reliable and reproducible results using these clear round-bottom immuno nonsterile 96-well plates, offered with a choice of surface.

Plates and Modules with Covalent Binding Surfaces (Thermo Scientific™)

Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces

Immuno Clear Standard Modules (Thermo Scientific™)

This product has been discontinued. Please consider Cat. No. 467679 as a replacement product.

Pierce™ Streptavidin Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 96-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, BlackS, 96-Well:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ 96-Well Polystyrene Plates, Corner Notch (Thermo Scientific™)

Thermo Scientific Pierce Clear 96-Well Microplates are made of specially formulated polystyrene and treated to produce high-capacity antibody-binding characteristics. These microplates have a corner notch for use in standard plate readers.

Features of Clear 96-Well Microplates:

• Clear plates for colorimetric ELISA and other assays
• Specially formulated optically clear polystyrene and treated to provide protein coating capacity
• High antibody-binding surface improves the overall performance in immunoassay applications
• Plates are certified for performance, having consistently low coefficients of variation among wells

Plate Properties:
• Antibody binding capacity: High, 400ng IgG/cm2 (1 cm2 = 100 µL volume)
• Binding interaction: hydrophobic-ionic
• Well-to-well coefficient of variation: CV ≤3%
• Well shape (bottom): Flat
• Maximum well volume: 360 µL

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Pierce™ 8-Well Polystyrene Strip Plates, Corner Notch (Thermo Scientific™)

Thermo Scientific Pierce 8-Well Strip Plates are optically clear, polystyrene plates that have a standard 96-well microplate footprint but also allow individual 8-well strips (i.e., plate columns) to be removed from the rigid frame.

Features of 8-Well Strip Plates:

• Special optically clear polystyrene formulation
• High antibody-binding surface improves the overall performance in immunoassay applications
• Plates are certified for performance, having consistently low coefficients of variation among wells
• Eight-well strips fit snugly in the plate frame, virtually eliminating strip loss while in use—even if turned upside down
• Strip guides help to avoid misorientation of the strip when returned to the holder
• Strips are gently removed from the frame with a strip-well ejector
• Wells can be easily separated from the strips with a gentle twist, allowing you to work with individual wells or strips of less than eight wells

The format provides convenience and quality-assurance when performing partial-plate assays because unused wells can be removed to prevent confusion and cross-contamination.

Plate Properties:
• Antibody binding capacity: High, 400ng IgG/cm2 (1 cm2 = 100 µL volume)
• Binding interaction: hydrophobic-ionic
• Well-to-well coefficient of variation: CV ≤3%
• Well shape (bottom): Flat
• Maximum well volume: 360 µL
• Packaging: 5 plates per sealed tray

Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates (Thermo Scientific™)

Perform automated applications more precisely with these round-bottom plates, which are available with and without cell culture treatment.

Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated White 384-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated White 384-Well Plates:

• White plates for chemiluminescent detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Black,384-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Black, 384-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 8-Well Strip Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ Protein A Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

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