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Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 96-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well

Pierce™ Maleimide Activated Plates, White, 96-Well (Thermo Scientific™)

These Thermo Scientific Pierce Maleimide Activated White, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.

Features of Maleimide Activated White, 96-Well Plates:

• White, for chemiluminescent assays
• Pre-blocked to reduce nonspecific binding
• Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl
• Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well
• Activation Level: 100 µL

Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent).

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Pierce™ Maleimide Activated Plates, Clear, 8-Well Strip
Pierce™ Maleimide Activated Plates, Black, 96-Well

Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity White 96-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Black, 384-Well

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 384-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip

Clear Flat-Bottom Immuno Nonsterile 384-Well Plates (Thermo Scientific™)

Carry out colormetric assays with reliable and reproducible results using these clear flat-bottom immuno nonsterile 384-well plates, offered with a choice of surface.

Immuno Breakable Modules Clear (Thermo Scientific™)

Choose from four surfaces and a variety of wells and configurations. Immuno Breakable Modules offer an optimal choice of products for the specific assay.

Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, White, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, White, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well

Pierce™ Protein G Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce White, Protein G Coated 96-Well Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

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Pierce™ Protein G Coated Plates, Clear, 96-Well
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Black, 96-Well

Pierce™ Streptavidin Coated Plates, Black, 384-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Black, 384-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, Black, 384-Well:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Black are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ Streptavidin Coated Plates, White, 96-Well
Pierce™ Streptavidin Coated Plates, Black, 96-Well

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are white for chemiluminescent assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well

QuantiGene™ Plex Magnetic Separation Plates (Invitrogen™)

QuantiGene Magnetic Separation Plates are 96-well flat-bottom plates used for the branched DNA and SAPE incubations on the second day of the QuantiGene Plex Assay. The Magnetic Separation Plates are compatible with the QuantiGene Handheld Magnetic Plate Washer (Cat. No. QP0702) and QuantiGene Plex Plate Seals (Cat. No. QG0524). These plates are one component of several included in the QuantiGene Plex Assay kit, and are only intended to supplement the assay kit when more plates are needed.

Pierce™ Protein L Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein L, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein L Coated Microplates:
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

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Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A/G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip

Pierce™ Nickel Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated Black 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These black plates can be used with fluorescent detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

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Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well

Pierce™ Streptavidin Coated High Capacity Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Clear, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Clear, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ Streptavidin Coated High Capacity Plates, Clear, 384-Well

Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, Black, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, Black, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

Related Products
Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well