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Pierce™ Nickel Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated Black 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These black plates can be used with fluorescent detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

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Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well

Pierce™ Protein A Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein A Coated Microplates:
• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b and IgG3
• Binds to Fc region of antibodies for optimal orientation

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Pierce™ Protein A Coated Plates, Clear, 96-Well
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A Coated Plates, White, 96-Well

QuantiGene™ Plex Magnetic Separation Plates (Invitrogen™)

QuantiGene Magnetic Separation Plates are 96-well flat-bottom plates used for the branched DNA and SAPE incubations on the second day of the QuantiGene Plex Assay. The Magnetic Separation Plates are compatible with the QuantiGene Handheld Magnetic Plate Washer (Cat. No. QP0702) and QuantiGene Plex Plate Seals (Cat. No. QG0524). These plates are one component of several included in the QuantiGene Plex Assay kit, and are only intended to supplement the assay kit when more plates are needed.

Pierce™ Glutathione Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

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Pierce™ Glutathione Coated Plates, Clear, 8-Well Strip
Pierce™ Glutathione Coated Plates, White, 96-Well

Pierce™ Glutathione Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well microplates coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins.

Features of these Glutathione Coated Plates:

Selective—specifically binds fusion proteins containing glutathione S-transferase
Capacity—binds approx. 10 ng GST protein per well
Versatile—ready-to-bind plate format is useful for protein interaction studies
Compatible—detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
Ready to use—Pre-blocked with BSA and SuperBlock Blocking Buffer to reduce nonspecific binding

Thermo Scientific Pierce Glutathione Coated Plates are standard 96-well polystyrene microplates whose wells have been coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These glutathione coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Alternatively, the plates can be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein (either protein interactions or a specific antibody). Because the plates are pre-blocked, initial purification of the cell lysates is not necessary.

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Pierce™ Glutathione Coated Plates, Clear, 8-Well Strip
Pierce™ Glutathione Coated Plates, Black, 96-Well

Pierce™ 8-Well Polystyrene Strip Plates, Corner Notch (Thermo Scientific™)

Thermo Scientific Pierce 8-Well Strip Plates are optically clear, polystyrene plates that have a standard 96-well microplate footprint but also allow individual 8-well strips (i.e., plate columns) to be removed from the rigid frame.

Features of 8-Well Strip Plates:

• Special optically clear polystyrene formulation
• High antibody-binding surface improves the overall performance in immunoassay applications
• Plates are certified for performance, having consistently low coefficients of variation among wells
• Eight-well strips fit snugly in the plate frame, virtually eliminating strip loss while in use—even if turned upside down
• Strip guides help to avoid misorientation of the strip when returned to the holder
• Strips are gently removed from the frame with a strip-well ejector
• Wells can be easily separated from the strips with a gentle twist, allowing you to work with individual wells or strips of less than eight wells

The format provides convenience and quality-assurance when performing partial-plate assays because unused wells can be removed to prevent confusion and cross-contamination.

Plate Properties:
• Antibody binding capacity: High, 400ng IgG/cm2 (1 cm2 = 100 µL volume)
• Binding interaction: hydrophobic-ionic
• Well-to-well coefficient of variation: CV ≤3%
• Well shape (bottom): Flat
• Maximum well volume: 360 µL
• Packaging: 5 plates per sealed tray

Immuno Breakable Modules Clear (Thermo Scientific™)

Choose from four surfaces and a variety of wells and configurations. Immuno Breakable Modules offer an optimal choice of products for the specific assay.

Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Mouse IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are white for chemiluminescent assays.

Features of Goat Anti-Mouse IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~7 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well

Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Rabbit IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are clear for colorimetric assays.

Features of Goat Anti-Rabbit IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~12 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, White, 96-Well
Pierce™ Goat Anti-Rabbit IgG Antibody Coated Plates, Black, 96-Well

Pierce™ Protein A/G Coated Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Clear, Protein A/G Coated 8-Well Strip Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein A/G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein A/G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein A/G Coated Microplates:
• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

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Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein L Coated Plates, Clear, 96-Well

Immuno Standard Modules, EB, black (Thermo Scientific™)

Conduct fluorescence- and luminescence-based immunoassays and binding assays using Thermo Scientific™ Immuno Standard Modules White and Black.

Pierce™ Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Sensitivity Plates,Black, 8-Well Strip are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.

Features of Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip

Sensitive—detect down to 5 ng/mL of biotinylated IgG
Specific—low background with high signal-to-noise ratios
Versatile—use either fluorescence, chemiluminescence or colorimetric detection
Robust—broad dynamic range
Ready to use—plates are supplied pre-blocked to save time

Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low non-specific interactions.

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Pierce™ Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip

Microlite™ White Microtiter™ Plates (Thermo Scientific™)

Experience consistent binding, high reflectivity, and low crosstalk for use in luminescent immunoassays and related techniques using Microlite Microtiter Plates.

Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Goat Anti-Mouse IgG Antibody Coated Plates are ideal for binding assays when available antibodies are in low quantities or they denature and become inactive upon direct adsorption to polystyrene plates. These 96-well plates are black for fluorescent assays.

Features of Goat Anti-Mouse IgG Antibody Coated Plates:

• Prevents antibody denaturation as a result of direct binding to polystyrene
• Unlike Protein A or Protein G Plates, these plates bind only to target species
• Antibody-binding capacity is higher than direct adsorption onto polystyrene
• Pre-blocked to reduce nonspecific binding
• Binding Capacity: ~7 pmol IgG/well
• Activation level: 100 µL/well

Because these plates are IgG-specific, purified antibodies are not required. These plates may be used for direct, indirect, competitive or sandwich assays.

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Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, Clear, 96-Well
Pierce™ Goat Anti-Mouse IgG Antibody Coated Plates, White, 96-Well

Pierce™ Streptavidin Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, Clear, 96-Well are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, BlackS, 96-Well:

• Approximate binding capacity is ~5 pmol biotin/well
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Streptavidin Coated Plates, Clear are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ Streptavidin Coated Plates, White, 96-Well
Pierce™ Streptavidin Coated Plates, Black, 384-Well