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Pierce™ Copper Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

These copper coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. Pierce Copper Coated Plates have high binding capacity because they are coating using an exclusive process that increases the amount of histidine-tagged protein that will bind to the plate surface. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range. The copper-coated plates are available as clear, white or black plates that can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Features of Copper Coated High Binding Capacity Plates:

• Quantitate previously undetectable histidine-tagged proteins
• Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
• Copper chelate provides greater binding capacity than nickel
• The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
• Pre-blocked for the ultimate convenience

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.

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Pierce™ Copper Coated High Capacity Plates, Clear, 96-Well
Pierce™ Copper Coated High Capacity Plates, Clear, 8-Well Strip
Pierce™ Copper Coated High Capacity Plates, Black, 96-Well

Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 384-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Coated High Capacity Clear 384-Well Plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Thermo Scientific Pierce Coated Plates:

Unique plate-coating technology—results in high loading and binding capacity
NeutrAvidin Protein—coated plates have up to twice the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 96-Well
Pierce™ NeutrAvidin™ Coated High Capacity Plates, Clear, 8-Well Strip

Pierce™ Nickel Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

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Pierce™ Nickel Coated Plates, Clear, 96-Well
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, Black, 96-Well

Complete Stainless Steel Rollout Drawer Set (Thermo Scientific™)

A convenient alternative to open-wire shelving

Pierce™ Streptavidin Coated High Capacity Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Capacity Plates, White, 96-Well are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

Features of Streptavidin Coated High Capacity Plates, White, 96-Well:

Unique plate-coating technology—results in high loading and binding capacity
Streptavidin coated option—plates have up to five times the binding capacity of typical plates
Broader dynamic range—extends the quantitative range so there is less need for dilutions
Save time—pre-blocked plates to reduce the number of assay steps
Flexible assay formats—coated plates offered in 8-well strips to reduce waste

The proprietary plate-coating technology used to manufacture these Thermo Scientific Pierce Coated Plates yields high-capacity plates with a wider detection limit than regular binding capacity plates. The standard curve exhibits greater linearity for detecting small biotinylated molecules such as peptides and oligonucleotides, resulting in greater assay precision.

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Pierce™ Streptavidin Coated High Capacity Plates, Black, 96-Well
Pierce™ Streptavidin Coated High Capacity Plates, White, 384-Well
Pierce™ Streptavidin Coated High Capacity Plates, Black, 384-Well

Pierce™ Streptavidin Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated Plates, White, 96-Well are pre-blocked and ready-to-use polystyrene 96-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of Streptavidin Coated Plates, White, 96-Well:

• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer

Thermo Scientific Pierce Streptavidin Coated Plates are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions.

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Pierce™ Streptavidin Coated Plates, Black, 96-Well
Pierce™ Streptavidin Coated Plates, Black, 384-Well

Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Black 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Black 96-Well Plates:

• Black plates for fluorescent detection methods
• Pre-blocked with Blocker BSA Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well
Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well

Pierce™ Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.

Features of Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip

Sensitive—detect down to 5 ng/mL of biotinylated IgG
Specific—low background with high signal-to-noise ratios
Versatile—use either fluorescence, chemiluminescence or colorimetric detection
Robust—broad dynamic range
Ready to use—plates are supplied pre-blocked to save time

Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low non-specific interactions.

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Pierce™ Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip

Pierce™ Protein G Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce White, Protein G Coated 96-Well Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

Features of Protein G Coated Plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

These plates are uniformly and stably coated with Protein G, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein G Coated Microplates:
• Binds strongly to IgG from many species including human, mouse, rabbit, sheep and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

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Pierce™ Protein G Coated Plates, Clear, 96-Well
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Pierce™ Protein G Coated Plates, Black, 96-Well

Pierce™ Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip (Thermo Scientific™)

Thermo Scientific Pierce Streptavidin Coated High Sensitivity Plates,Black, 8-Well Strip are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.

Features of Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip

Sensitive—detect down to 5 ng/mL of biotinylated IgG
Specific—low background with high signal-to-noise ratios
Versatile—use either fluorescence, chemiluminescence or colorimetric detection
Robust—broad dynamic range
Ready to use—plates are supplied pre-blocked to save time

Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low non-specific interactions.

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Pierce™ Streptavidin Coated High Sensitivity Plates, Clear, 8-Well Strip

Pierce™ Nickel Coated Plates, Clear, 96-Well (Thermo Scientific™)

Thermo Scientific Pierce Nickel Coated Clear 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These clear plates can be used with colorimetric detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

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Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well
Pierce™ Nickel Coated Plates, Black, 96-Well

Immuno Clear Standard Modules, Max. working volume: 330µL (Thermo Scientific™)

Immuno Clear Standard Modules feature Nunc™, Immulon™ and Microtiter™ module plates that are developed for ELISA techniques and fit standard equipment.

Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated Black 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated Black 96-Well Plates:

• Black plates for fluorescent detection methods
• Pre-blocked with SuperBlock Blocking Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well
Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well

Pierce™ NeutrAvidin™ Coated Plates, White, 96-Well (Thermo Scientific™)

Thermo Scientific NeutrAvidin Protein Coated White 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Features of NeutrAvidin Protein Coated White 96-Well Plates:

• White plates for chemiluminescent detection methods
• Pre-blocked with Blocker BSA Buffer
• Easy and gentle immobilization of biotin-containing conjugates
• Low nonspecific binding
• No denaturing of the protein component of a conjugate upon binding
• Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
• Less expensive than streptavidin coated plates
• Lowest nonspecific binding properties of all biotin-binding proteins

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

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Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well
Pierce™ NeutrAvidin™ Coated Plates, White, 384-Well
Pierce™ NeutrAvidin™ Coated Plates, Black, 384-Well

Pierce™ Biotin-Binding Plate Sample Pack (Thermo Scientific™)

The Thermo Scientific Pierce Biotin-Binding Plate Sample Pack is ideal for establishing which variety of streptavidin coated plate is best for particular assay involving biotinylated molecules.

The Biotin-Binding Plate Sample Pack helps in assay development. This sample pack contains streptavidin and NeutrAvidin Protein coated on microplates that are preblocked with either SuperBlock, Blocking Buffer or Blocker BSA. This variety allows researchers to determine which plate produces the best signal-to-noise ratio for a specific experimental system. Once optimal conditions are empirically determined, the plate that provides the best results can be purchased in bulk for the remainder of the study.