Shop All Affinity Columns, Media, & Standards

CaptureSelect™ IgE Affinity Matrix (Thermo Scientific™)

Thermo Scientific CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.

Features of this affinity matrix include:
• Purification of human IgE, binding different recombinant IgE forms
• Efficient removal of host cell proteins (HCP) and IgE aggregates
• Excellent scalability
• Non–animal-derived

Free of animal components
CaptureSelect products contain affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprised of three complementarity determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose bead
Average particle size: 65 µm
Ligand: CaptureSelect IgE affinity ligand
Ligand coupling method: aldehyde coupling
Binding capacity: >5 mg/mL resin
Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
Formulation buffer: 20% (v/v) ethanol

HisPur™ Cobalt Purification Kit, 0.2 mL (Thermo Scientific™)

The Thermo Scientific HisPur Cobalt Purification Kit contains 0.2-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.

Features of HisPur Cobalt Resin:

High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
Cost effective—resin can be reused several times or discarded after single use
Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant protein that can be efficiently recovered by mild imidazole elution. Greater than 10 mg of His-tagged protein can be bound per milliliter of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate.

Various His-tagged proteins can be recovered in similar or higher purity compared to that obtained from other available nickel or cobalt IMAC resins using either a spin or gravity-flow column format. Purification performance of HisPur Cobalt Resin was benchmarked against IMAC resins from other suppliers using multiple His-tagged proteins of different sizes and expression levels. The results demonstrate that HisPur Cobalt Resin achieves maximal protein purity without sacrificing protein yield.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Cobalt Resin
HisPur™ Cobalt Spin Columns, 0.2 mL

HisPur™ Cobalt Purification Kit, 1 mL (Thermo Scientific™)

The Thermo Scientific HisPur Cobalt Purification Kit contains 1-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.

Features of HisPur Cobalt Resin:

High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
Cost effective—resin can be reused several times or discarded after single use
Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant protein that can be efficiently recovered by mild imidazole elution. Greater than 10 mg of His-tagged protein can be bound per milliliter of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate.

Various His-tagged proteins can be recovered in similar or higher purity compared to that obtained from other available nickel or cobalt IMAC resins using either a spin or gravity-flow column format. Purification performance of HisPur Cobalt Resin was benchmarked against IMAC resins from other suppliers using multiple His-tagged proteins of different sizes and expression levels. The results demonstrate that HisPur Cobalt Resin achieves maximal protein purity without sacrificing protein yield.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Cobalt Resin
HisPur™ Cobalt Spin Columns, 1 mL

CaptureSelect™ TSH Affinity Matrix (Thermo Scientific™)

CaptureSelect TSH Affinity Matrix was specifically designed for the purification of thyroid-stimulating hormone (TSH) from recombinant sources.

Main characteristics:

Matrix: agarose bead
Average particle size:
65 µm
Ligand: CaptureSelect TSH affinity ligand
Ligand coupling method: epoxide
Binding capacity: >5 mg/mL of resin
Elution conditions: 0.1 M citrate buffer, pH 3.0
Flow characteristics: 75–200 cm/h (up to 1 bar)
Formulation buffer: 20% (v/v) ethanol

Some features of CaptureSelect TSH Affinity Matrix include:
• Highly selective for thyroid-stimulating hormone with no affinity for the other gonadotropins (FSH, LH, and HCG)
• Enables efficient purification of recombinant TSH with high yields and retained activity
• Offers excellent scalability
• Non–animal-derived

The high selectivity and yields obtained using CaptureSelect TSH Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

HisPur™ Cobalt Purification Kit, 3 mL (Thermo Scientific™)

The Thermo Scientific HisPur Cobalt Purification Kit contains 3-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.

Features of HisPur Cobalt Resin:

High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
Cost effective—resin can be reused several times or discarded after single use
Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant protein that can be efficiently recovered by mild imidazole elution. Greater than 10 mg of His-tagged protein can be bound per milliliter of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate.

Various His-tagged proteins can be recovered in similar or higher purity compared to that obtained from other available nickel or cobalt IMAC resins using either a spin or gravity-flow column format. Purification performance of HisPur Cobalt Resin was benchmarked against IMAC resins from other suppliers using multiple His-tagged proteins of different sizes and expression levels. The results demonstrate that HisPur Cobalt Resin achieves maximal protein purity without sacrificing protein yield.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Cobalt Resin
HisPur™ Cobalt Spin Columns, 3 mL

AminoLink™ Coupling Resin (Thermo Scientific™)

Thermo Scientific AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

Features of AminoLink Coupling Resin:

AminoLink Coupling Resin—aldehyde-activated crosslinked, 4% beaded agarose
• Ideal for antibodies and other proteins—immobilize molecules via primary amines (-NH2)
Flexible coupling conditions—efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
Stable, permanent immobilization—Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose—bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Versatile and reusable—prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Proteins and other molecules with primary amines can be covalently attached (immobilized) to AminoLink Resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

The AminoLink Resin immobilization reaction involves spontaneous formation of Schiff base bonds between aldehydes and amines and their subsequent stabilization by incubation with a mild reductant. The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20 mg of immobilized protein per milliliter of resin.

Related Products
AminoLink™ Immobilization Kit
AminoLink™ Reductant

Pierce™ Dye Removal Columns (Thermo Scientific™)

Thermo Scientific Pierce Dye Removal Columns effectively bind to unconjugated fluorescent dye molecules from protein solutions to rapidly purify fluorescent conjugated antibodies and other proteins after labeling reactions.

These Fluorescent Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions. Removing excess dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The dye removal resin in this kit is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated dye. Using the appropriate amount of resin and buffer conditions, almost any fluorescent dye can be removed with this kit.

Features of Dye Removal Columns:

Fast—removes free, unconjugated dye from labeled protein solutions in less than a minute
Specific—purification resin provides outstanding conjugate recovery (75 to 95%)
Customizable—separate resin and microcentrifuge columns allow optimization for different dyes and concentrations of sample
Sample-friendly—processing results in minimal sample dilution

This product has been used successfully to remove excess, unconjugated fluorescein, rhodamine and DyLight Dyes from antibodies following labeling reactions. Samples with dyes in the green spectrum, such as Dylight 549 and rhodamine, might require double processing because they require a high molar excesses for labeling. When using fluorescent dyes other than those described in the product instructions, the ratio of resin to protein/dye mixture will require optimization.

POROS™ MC 20 µm Column, 2.1 x 30 mm, 0.1 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Immobilized Metal Affinity Column. POROS™ MC is based on a Imido-diacetate functional group designed for the purifiction of proteins that form co-ordination complexes via metal sites and specific amino acids, such as histidine tagged fusion proteins.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

CaptureSelect™ Kappa XL Pre-packed Column (Thermo Scientific™)

CaptureSelect™ KappaXL Affinity Matrix, available here in a pre-packed column format, has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.

Features of this affinity matrix include:
• Mild elution for Fab fragments and antibodies
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ KappaXL affinity ligand
Coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~30 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid, pH 3.5
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

HisPur™ Cobalt Chromatography Cartridges, 1 mL (Thermo Scientific™)

Thermo Scientific HisPur Cobalt Chromatography Cartridges contain a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.

Features of HisPur Cobalt Resin:

High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
Cost effective—resin can be reused several times or discarded after single use
Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates

Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.

HisPur Cobalt IMAC Resin specifically binds His-tagged recombinant protein that can be efficiently recovered by mild imidazole elution. Greater than 10 mg of His-tagged protein can be bound per milliliter of resin using a 28 kDa fusion protein expressed and purified from an E. coli lysate.

Various His-tagged proteins can be recovered in similar or higher purity compared to that obtained from other available nickel or cobalt IMAC resins using either a spin or gravity-flow column format. Purification performance of HisPur Cobalt Resin was benchmarked against IMAC resins from other suppliers using multiple His-tagged proteins of different sizes and expression levels. The results demonstrate that HisPur Cobalt Resin achieves maximal protein purity without sacrificing protein yield.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, and 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Cobalt Chromatography Cartridges, 5 mL
HisPur™ Cobalt Spin Columns, 1 mL
HisPur™ Cobalt Resin

POROS™ 20 G Self-Pack™ Protein G Affinity Resin (Thermo Scientific™)

Applied Biosystems™ POROS™ Protein G Affinity Media. Contains enough packing for three 4.6 mm x 100 mm columns or six 4.6 mm x 50 mm columns. POROS™ Protein G is based on an immobilized recombinant Protein G functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ Self Pack™ Column Packing Technology

Self Pack Technology allows you to pack your own high performance POROS columns at a fraction of the cost of prepacked columns. As such, it represents the most cost-effective way to screen the range of POROS resins for the one best suited for the separation problem at hand. The Self Pack Technology consists of four elements (each sold separately): a packing device, an empty column, POROS resins and an appropriate test standard.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Pierce™ GST Spin Purification Kit, 3 mL (Thermo Scientific™)

This Thermo Scientific Pierce GST Spin Purification Kit contains Pierce Glutathione Spin Columns and buffer components to provide a fast, easy-to-use format for purifying GST-fusion proteins from cellular lysates.

Features of Glutathione Agarose:

High capacity—binds at least 40 mg of purified recombinant GST protein per milliliter of resin
High yield and purity—consistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity
Cost-effective—resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance
Versatile—works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions
Compatible—validated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures

The glutathione is immobilized through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification. Whether the purpose is to purify large amounts of recombinant protein from over-expressing E. coli lysates or to investigate protein interactions involving GST-tagged bait proteins, Pierce Glutathione Agarose is suitable for the task.

Thermo Scientific Pierce Glutathione Agarose is also offered in three volumes of resin slurry, complete GST purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs.

Pierce Glutathione Agarose effectively purifies high levels of overexpressed GST-tagged fusion proteins from bacterial lysates, such as those that are obtained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents.

Pierce Glutathione Agarose performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular GSH resins from other suppliers.

Pierce Glutathione Agarose is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or ligand leaching during normal use.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags or small proteins, such as glutathione S-transferase (GST). This protein binds specifically to reduced glutathione (GSH) in near-neutral, nondenaturing conditions (e.g., Tris buffer). Bound protein is easily dissociated (eluted) by competitive displacement with buffer containing free, reduced GSH (oxidized glutathione, GSSH is not effective for this purpose). When proteins of interest are expressed as fusions with GST and glutathione is immobilized to an solid support, this protein-substrate system enables affinity purification of recombinant proteins, as well as various other experiments with those proteins.

Related Products
Pierce™ Glutathione Agarose
Pierce™ Glutathione Spin Columns, 3 mL

POROS™ MC 20 µm Column, 4.6 x 50 mm, 0.8 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Immobilized Metal Affinity Column. POROS™ MC is based on a Imido-diacetate functional group designed for the purifiction of proteins that form co-ordination complexes via metal sites and specific amino acids, such as histidine tagged fusion proteins.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

CaptureSelect™ KappaXP Pre-packed Column (Thermo Scientific™)

CaptureSelect KappaXP Affinity Matrix is offered here in a pre-packed format as a set of 5 x 1 mL columns or as a 1x 5 mL column, with connectors that are compatible with standard chromatography equipment.

CaptureSelect KappaXP Affinity Matrix is an improved version of CaptureSelect KappaXL Affinity Matrix, designed for higher binding capacity. The selectivity is the same; the ligand binds the constant domain of the human kappa light chain, but it features an increased binding capacity (up to 45 g IgG/L) and a wider elution range (full elution up to pH 6.0) compared to other affinity resins that bind the kappa light chain.

Features of this affinity matrix include:
• Single-step purification of Ig’s, Fab, and Fab2 fragments directly from complex source materials
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect KappaXP Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

POROS™ MabCapture™ A Affinity Chromatography Resin (Thermo Scientific™)

Applied Biosystems™ POROS™ MabCapture™ A Media is a new design in Protein A chromatography resins technology, combining improvements made in bead and chemistry technology. These advances provide a monoclonal antibody purification resins that not only demonstrates the highest dynamic binding performance at low flow rates but also one that can maintain superior binding capacity at high linear velocities where other chromatography resins products cannot operate.
With POROS™ MabCapture™ affinity chromatography resins you can:
• Obtain the highest dynamic binding capacity for monoclonal antibodies vs. conventional agarose or silica based Protein A medias
• Maintain high dynamic binding capacity at flow rates over 700cm⁄hr
• Design processes with greater flexibility (shorter bed heights, faster flow rates or both) due to the improvements made to the antibody binding efficiency
• Clean and sanitize with standard sodium hydroxide agents utilized for other chromatography products
• Recognize major increases to process productivity and reduce the downstream bottleneckThe development of humanized monoclonal antibodies has continued to fuel the growth of the biotherapeutic market. Addressing the therapeutic demand for monoclonal antibodies has lead to major improvements to the cell based expression systems that are used to manufacture these proteins. Consequently, these improvements have moved the processing bottleneck from upstream cell expression to the downstream purification process, as the purification operations struggle to keep up with the large quantities of antibodies that need to be manufactured. Protein A chromatography, which is used as the primary capture chromatography step in most antibody process platforms, is generally regarded as the major process bottleneck due to the slow flow rates and long residence times required for the effective operation of conventional Protein A chromatography medias.The introduction of POROS™ MabCapture™ chromatography resins is aimed at cracking open the downstream bottleneck and providing the capacity, efficiency and flexibility needed to make improvements to the downstream purification platform resulting in major increases to process productivity and antibody manufacturing.
Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.