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Pierce™ Thiophilic Adsorption Kit (Thermo Scientific™)

Thermo Scientific Pierce Thiophilic Adsorbent is a protein-free immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human. This Pierce Thiophilic Adsorption Kit is a purification kit containing columns pre-packed with the resin and ready-to-use buffers.

Features of Thiophilic Adsorbent:

High capacity – gently bind and purify approx. 20 mg polyclonal antibody per milliliter of adsorbent resin
Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY
Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps
Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody

Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity.

Thiophilic adsorption is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that contains a sulfone group in close proximity to a thioether. Salts that interact with water molecules, such as potassium and ammonium sulfate, promote this binding by driving the protein into close proximity with the ligand.

Related Products
Pierce™ Thiophilic Adsorbent

Glutathione Agarose, Linked Through Sulfur, sedimented bead suspension (Invitrogen™)

Glutathione agarose prepared by linking the sulfur atom of glutathione to crosslinked beaded agarose can be used for purification of GST fusion proteins. Each milliliter of gel can bind approximately 5-6 mg of boivine-liver GST. Adding excess free glutathione liberates the GST fragment from the matrix which can then be regenerated by washing with a high-salt buffer.

Pierce™ Protein G UltraLink™ Resin (Thermo Scientific™)

Thermo Scientific Pierce Protein G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein G has been immobilized for use in IgG purification methods.

Features of Protein G UltraLink Resin:

Immobilized Protein G – specifically binds IgG from several mammalian species, especially all isotypes of IgG from human, mouse and rat
Standard capacity – loading of immobilized Protein G provides a binding capacity of approx. 20 mg human IgG per milliliter of resin
UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

The Protein G UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein G is a popular antibody-binding protein that is especially useful for affinity purification of IgG from mouse, human and rat serum.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Properties of Thermo Scientific UltraLink Resin:
• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2 mL per gram of beads (> 60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000 cm per hour
• Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Products
Pierce™ Protein G Plus UltraLink™ Resin
Pierce™ Protein G UltraLink™ Columns, 2 mL
Pierce™ Protein G Agarose

Pierce™ Chicken IgY Purification Kit (Thermo Scientific™)

The Thermo Scientific Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Features of the Chicken IgY Purification Kit:

More for your money—purifies twice the amount of IgY than the leading competitor's kit with a lower cost-per-mg of IgY purified
High recovery and purity—obtain 80 to 120 mg IgY per egg yolk at 85% to 95% purity by SDS-PAGE analysis
Ease-of-use—simple precipitation method purifies chicken IgY without affinity columns
Trouble-free method—fewer steps than other IgY purification kits
Flexibility—eggs can be stored in buffer and purified at a later date
Convenience—use eggs directly out of refrigerator; no need to wait for them to warm up

Typical IgG affinity resins (Protein A or Protein G) do not bind chicken IgY and therefore are not effective for IgY purification. The Pierce Chicken IgY Purification Kit uses a delipidation-and-precipitation procedure that is optimized to recover approximately 100 mg of 90% pure IgY from fresh egg yolks of appropriately immunized chickens. The resulting polyclonal IgY is sufficiently pure for direct use in many immunodetection procedures, or it can be easily affinity-purified against specific antigen because it is dissolved in PBS and thoroughly clarified.

Affinity chromatography with Protein A or Protein G cannot be used to purify IgY from chicken serum or egg yolk because this species of immunoglobulins are not recognized by the Protein A, G or L binding sites. However, the Pierce Chicken IgY Purification Kit provides a simple and reliable method for recovering large amounts of chicken IgY from egg yolk with our unique delipidation reagent that separates the proteins from lipid. (The egg yolk can be stored in the delipidation reagent a year or longer before processing.) After lipid separation, simply mix the sample with the IgY Precipitation Reagent which causes the IgY to separate from the lipid fraction during centrifugation. The precipitated chicken IgY is then easily recovered by dissolution in phosphate-buffered saline (PBS).

Chicken IgY vs. Mammalian IgG:
The terms IgG and IgY are commonly interchanged when referring to chicken immunoglobulin. Immunoglobulins from chickens and other avian species bear some resemblance to mammalian IgG, but also display some unique structural and functional characteristics that distinguish them from IgG. IgY is the accepted/proper term for chicken antibodies. Chicken IgY is the functional equivalent to mammalian IgG. It is found in the serum of chickens and is passed from the mother chicken to the embryo via the egg yolk, imparting a high concentration of chicken IgY to developing embryo.

Advantages of Chicken IgY:
Chicken IgY immunoglobulins can be harvested from the egg yolk as well as serum and blood. There are many advantages to using these antibodies:

• Unlike mice, rabbits or other mammals, hens elicit a strong antibody response against highly conserved mammalian protein sequences
• Chicken antibodies can be obtained without sacrificing or bleeding the animal—simply collect the eggs and use the Pierce Chicken IgY Purification Kit
• Each yolk can yield 100-150 mg of antibody.
• Chicken IgY has less cross-reactivity toward mammalian proteins—does not bind Fc receptors!

CaptureSelect™ Apolipoprotein H Affinity Matrix (Thermo Scientific™)

CaptureSelect™ Apolipoprotein H Affinity Matrix is one of a group of CaptureSelect™ protein purification affinity resins developed for the simple, single-step purification of variety human plasma and serum proteins. This unique selection of affinity purification ligands can capture proteins from either recombinant or native sources with a high degree of affinity and specificity and are immobilized on a high-capacity agarose support.

POROS™ CaptureSelect™ AAV8 Affinity Resin (Thermo Scientific™)

POROS™ CaptureSelect™ AAV8 high performance affinity resin is engineered to address the high selectivity and capacity requirements needed for the purification of viral vectors used for gene therapy applications and is specifically intended for the purification of adeno-associated virus type 8 (AAV8).

Features of POROS CaptureSelect AAV8 resin include:
• One-step AAV8 purification from crude material with high purity and yield
• High specificity and capacity, which helps reduce the process volume significantly for subsequent steps and maximizes yield
• Low operating back pressure and linear pressure versus flow responses driving flexible scalability
• Superior capacity maintained at high flow rates
• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, with superior physical and chemical stability enabling a more robust downstream purification process
• Non-animal derived—the ligand is a 13-kDa single-domain fragment that comprises the 3 CDRs that form the antigen-binding domain and is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

POROS CaptureSelect AAV8 affinity resin specifically binds AAV8 and enables a robust, fast, and efficient purification process with excellent purity and yield obtained in one step. The use of this affinity resin will enable significant improvement to downstream processing by reducing the purification steps and maximizing productivity, for scalability and processing consistency.

Main characteristics:

Matrix: cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect AAV8 affinity ligand
Ligand coupling method: carbonyldiimidazole (CDI)
Binding capacity: >1x1013 genome copies/mL of resin
Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Formulation buffer: 18% (v/v) ethanol

CaptureSelect™ Factor X Affinity Matrix (Thermo Scientific™)

CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

Features of the CaptureSelect Factor X Affinity Matrix include:
• Highly selective for human Factor X with no affinity for other human plasma proteins
• Binds both Factor X and Factor Xa in the presence of calcium
• Enables efficient purification of Factor X with high yields and retained activity
• Mild elution at neutral pH using EDTA
• Offers excellent scalability
• Non-animal-derived

Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.

View other biosimilars and recombinant protein affinity matrices ›

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 14-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect Factor X affinity ligand
Ligand coupling method: epoxide
Binding capacity: >10 mg/mL resin
Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
Flow characteristics: 50–150 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

POROS™ A 20 µm Column, 4.6 x 50 mm, 0.8 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Protein A, Affinity Column. POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

SulfoLink™ Coupling Resin (Thermo Scientific™)

Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

Features of SulfoLink Coupling Resin:

Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
Fast—spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
High capacity—Immobilize 1 to 2 mg of peptide or 2 to 20 mg of protein per 2 mL column of SulfoLink Coupling Resin

When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest. Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Related Products
SulfoLink™ Immobilization Kit for Peptides, 2 mL
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL

HiPPR™ Detergent Removal Spin Column Kit (Thermo Scientific™)

Thermo Scientific HiPPR Detergent Removal Spin Column Kit is used to improve mass spectrometry results by efficiently removing detergents from 25 to 200 µL samples with low protein or peptide concentrations.

Features of HiPPR Detergent Removal Spin Column Kit:

Optimized—removes >95% of detergent from samples with low-concentrations (1 to 100 µg/mL) of proteins or peptides
Fast—sample processing takes less than 15 minutes
Effective—eliminates detergent-interference in downstream applications like ELISA, isoelectric focusing and mass spectrometry

Available Formats:
Resin Slurries—bulk resin supplied with empty 0.8 mL spin columns
Spin Columns—0.1 mL micro-centrifuge columns
96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.

Optimized for sample concentrations of 1 to 100 µg/mL,the HiPPR (High Protein and Peptide Recovery) Detergent Removal Resin removes >95% of detergents used in protein extraction and biological sample preparation with minimal loss of protein and peptide. The Detergent Removal Resin is ideal for removing commonly used detergents, including SDS, Triton™ X-100, NP-40 and CHAPS, at concentrations of 0.5-1% and is available in pre-filled spin columns and 96-well filter spin plates for sample volumes up to 100 µL. For other sample sizes, the detergent removal resin slurry is available with empty spin columns that can be used to make custom spin columns for processing sample volumes of 25 to 200 µL.

The detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis because even low concentrations of detergents will contaminate instruments and interfere with column binding, elution and peptide ionization. The HiPPR Detergent Removal Resin is ideal for rapid detergent removal from tryptic digests to improve the results of LC-MS/MS and MALDI-MS analysis as to help maintain column and instrument performance over time.

Related Products
HiPPR™ Detergent Removal Spin Columns, 0.1 mL
HiPPR™ Detergent Removal 96-well Spin Plates, 0.1 mL
Pierce™ Detergent Removal Resin

Pierce™ NHS-Activated Agarose, Dry (Thermo Scientific™)

Thermo Scientific Pierce NHS-Activated Agarose is a high-quality, amine-reactive, beaded-agarose resin for rapid and stable immobilization of proteins, peptides and other ligands via primary amines.

Features of NHS-Activated Agarose:

Easy to use—immobilize in a simple one-step reaction with minimal hands-on time
Rapid and efficient—greater than 85% coupling for most proteins within 30 minutes
Innovative format—the dry agarose concentrates the sample, making it ideal for immobilizing dilute proteins
Safe—No hazardous chemicals needed (e.g., sodium cyanoborohydride, cyanogen bromide)
Versatile—affinity resin is adaptable to column and batch affinity chromatography techniques and FPLC applications
Compatible—use with any primary amine-containing compound
Reusable—the leak-resistant chemistry means you can reuse the affinity resin
High binding capacity—coupling capacity of >25 mg/mL (dry resin)

NHS-activated agarose is crosslinked, 6% beaded agarose resin that contains N-hydroxysuccinimide (NHS) functional groups. The activated resin reacts with primary amines to form stable amide linkages that covalently immobilize antibodies or other proteins for use in affinity purification procedures. Pierce NHS-Activated Agarose has a coupling capacity greater than 25 mg/mL for the reconstituted dry resin.

Applications:
• Rapid Immobilization of goat anti-mouse and anti-rabbit antibodies in order to purify IgG produced in animals or hybridomas.
• Bulk immobilization of Protein A for purification of monoclonal antibodies
• Immobilization of ligands for purification of recombinant proteins

Pierce NHS-Activated Agarose resin uses reliable NHS-ester Chemistry and does not require hazardous chemicals for immobilization. Other amine-reactive supports, such as periodate-oxidized resins, use toxic sodium cyanoborohydride to stabilize the reaction linkage to primary amines and take 4 to 6 hours to complete. Traditional methods such as cyanogen bromide-activated supports also couple amines; however, this chemistry results in nonspecific binding and constant slow leakage of the coupled ligand. Reactions with Pierce NHS-Activated Agarose are complete in less than one hour and yield much more stable linkages.

The NHS-Activated Agarose coupling reaction is performed in an amine-free buffer at pH 7-9. Protein coupling efficiency is typically greater than 80%, regardless of the ligand's molecular weight or pI. Once a ligand is immobilized, the prepared resin can be used for multiple affinity purification procedures. The crosslinked beaded agarose has fast linear flow potential, making it useful for gravity-flow and low- to medium-pressure applications.

The unique dry form does not require storage in or removal and disposal of the acetone solvent. In addition, the dry resin is ideal for coupling reactions with dilute samples because it concentrates the sample as the resin swells, reducing the volume of the starting material and resulting in highly efficient ligand immobilization.

Related Products
Pierce™ NHS-Activated Agarose Slurry
Pierce™ NHS-Activated Agarose Spin Columns, 2 mL
Pierce™ NHS-Activated Agarose Spin Columns, 0.2 mL

CaptureSelect™ tPA Affinity Matrix (Thermo Scientific™)

CaptureSelect tPA Affinity Matrix has been specifically designed for the purification of recombinant human tissue plasminogen activator (tPA). This matrix can be used for the purification of recombinant human tPA from cell culture harvests with high purity and yield in a single step. The design of the tPA affinity matrix allows elution of the tPA protein at a neutral pH in the presence of magnesium chloride, but standard low-pH elution buffers like 0.1 M glycine, pH 3.0, can also be used.

Features of this affinity matrix include:
• Neutral pH elution with 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0
• Caustic cleaning with 50 mM NaOH
• Excellent scalability
• Non-animal-derived

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa fragment comprising the 3 complementarity-determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect tPA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 8-10 g/L depending on flow rate, column height, and contact time
Elution conditions: Neutral pH: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ C-tagXL Pre-packed Column (Thermo Scientific™)

CaptureSelect C-tagXL Affinity Matrix is an improved version of CaptureSelect C-tag Affinity Matrix with a significantly increased binding capacity, offered here in pre-packed format.

Like C-tag Affinity Matrix, C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

The affinity matrix is offered in a pre-packed format as a set of 5 x 1 mL columns or as one 5 mL column, with connectors that are compatible with standard chromatography equipment.

POROS™ CaptureSelect™ CH1-XL Affinity HPLC Column, 4.6 x 50 mm (Thermo Scientific™)

POROS CaptureSelect CH1-XL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands. CaptureSelect CH1-XL Affinity HPLC columns recognize the CH1 domain of human IgG antibodies, enabling quantitation and small-scale purification of all IgG subclasses (IgG1, IgG2, IgG3, and IgG4), as well as Fab fragments and fusion proteins containing the intact CH1 domain, independent of light-chain iso-type and source material.Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.

Features of POROS CaptureSelect CH1-XL Affinity HPLC columns include:
Versatile—applicable for IgG Fab fragments, all human IgG subclasses, and IgG fusion proteins with intact CH1 domain
Specific—no cross-binding to bovine IgG
Fast—POROS resins enable quantitation or purification in minutes
Hands-off operation—HPLC method can be automated for a streamlined operation
Robust—designed to deliver high-performance purification over hundreds of chromatography cycles

High speed with high specificity
POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS Affinity HPLC columns are for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligand enables one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regimen results in strong re-use performance. POROS Affinity HPLC columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

Main characteristics

Protein binding capacity: 26.2 mg/mL for human IgG; 8.8 mg/ml human Fab fragment
Species specificity: human
Column material: PEEK polymer
Frit: 2 µm, titanium
POROS resin: cross-linked poly(styrene-divinylbenzene)
CaptureSelect ligands: single-domain monospecific antibody fragment produced in Saccharomyces cerevisiae

CaptureSelect™ hCG Affinity Matrix (Thermo Scientific™)

The CaptureSelect™ hCG Affinity Matrix was specifically designed for the purification of human chorionic gonadotropin (hCG) from recombinant sources.

Some features of the CaptureSelect hCG Affinity Matrix include:
• Selective for hCG, with cross-binding to LH, FSH, and TSH
• Enables efficient purification of recombinant gonadotropins with high yields and purity
• Offers excellent scalability
• Non–animal-derived

Human gonadotropins (hCG, LH, FSH, TSH) are glycoprotein hormones secreted by gonadotrope cells of the pituitary gland that are often produced as recombinant proteins. They have an identical alpha chain subunit that is recognized by the ligand. Consequently, the CaptureSelect hCG Affinity Matrix shows binding to all human gonadotropins. The high selectivity and yields obtained using CaptureSelect hCG Affinity Matrix enable a robust and efficient purification process for hCG with excellent purity obtained in one step.

Free of animal components
CaptureSelect affinity ligands are created with a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect hCG affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: approx. 4 mg/mL resin (determined with HCG)
Elution conditions: 20 mM citric acid, pH 3.0
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20% (v/v) ethanol