Shop All Affinity Columns, Media, & Standards

Pierce™ Protein G Plus Agarose Thermo Scientific™

Thermo Scientific Pierce Protein G Plus Agarose is a high-capacity Protein G beaded agarose resin for use in a variety of antibody affinity purification methods.

Features of Protein G Plus Agarose:

Protein G – immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein G by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – this “Plus” variety of Pierce Protein G Agarose has a dense load of immobilized Protein G, providing a binding capacity greater than 20 mg human IgG/mL resin

Pierce Protein G Plus Agarose consists of recombinant Protein G that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). The resin is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G is especially well suited for use with mouse antibodies (including mouse IgG1) in addition to most IgG isotypes from human, goat and sheep samples.

Protein G is a bacterial cell wall protein original from group G Streptococcus and now produced as a recombinant in E. coli. Like Protein A, Protein G binds to most mammalian immunoglobulins primarily through their Fc regions. Native Protein G contains two immunoglobulin binding sites, as well as albumin and cell surface binding sites. In the recombinant form of Protein G, these albumin and cell surface binding sites have been eliminated to reduce nonspecific binding when purifying immunoglobulins. Recombinant Protein G has a mass of approximately 21.6kDa, but its apparent size by SDS-PAGE is 31 to 34kDa. IgG-binding function is optimal at pH 5 but also occurs efficiently in near-neutral conditions (pH 7.0 to 7.2).

Compared to Protein A, Protein G binds a broader spectrum of IgG subclasses from human, mouse and rat serum. In particular, besides binding other isotypes just as well as Protein A, Protein G exhibits stronger binding to human IgG3, mouse IgG1 and all three isotypes of Rat IgG. Thus, Protein G is generally recommended for applications involving these species and isotypes of antibody. With regard to other species, Protein G provides higher-capacity binding for IgG from cow, goat and sheep, but it binds more weakly than Protein A to pig, guinea pig, dog and cat IgG.

Pierce Protein G Plus Agarose is prepared using Thermo Scientific AminoLink* Coupling Chemistry, which provides several advantages compared to traditional methods of ligand immobilization. AminoLink Immobilization results in conjugation between sugar monomers of the agarose beads and native lysine residues on the Protein A via simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization, the AminoLink Method does not introduce any novel chemical groups that could cause undesired nonspecific binding and produces a stable, essentially irreversible bond. The result is a high-binding-capacity resin that retains functional immobilized Protein G through multiple rounds of antibody purification.

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Related Products
Pierce™ Protein G Agarose

Pierce™ Dye Removal Columns Thermo Scientific™

Thermo Scientific Pierce Dye Removal Columns effectively bind to unconjugated fluorescent dye molecules from protein solutions to rapidly purify fluorescent conjugated antibodies and other proteins after labeling reactions.

These Fluorescent Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions. Removing excess dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The dye removal resin in this kit is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated dye. Using the appropriate amount of resin and buffer conditions, almost any fluorescent dye can be removed with this kit.

Features of Dye Removal Columns:

Fast—removes free, unconjugated dye from labeled protein solutions in less than a minute
Specific—purification resin provides outstanding conjugate recovery (75 to 95%)
Customizable—separate resin and microcentrifuge columns allow optimization for different dyes and concentrations of sample
Sample-friendly—processing results in minimal sample dilution

This product has been used successfully to remove excess, unconjugated fluorescein, rhodamine and DyLight Dyes from antibodies following labeling reactions. Samples with dyes in the green spectrum, such as Dylight 549 and rhodamine, might require double processing because they require a high molar excesses for labeling. When using fluorescent dyes other than those described in the product instructions, the ratio of resin to protein/dye mixture will require optimization.

POROS™ CaptureSelect™ FSH Affinity Column, 2.1 x 30 mm Thermo Scientific™

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ FSH HPLC columns incorporate the advantages of high-speed POROS™ chromatography resins with the unique selectivity of CaptureSelect™ ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

• Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ FSH affinity ligand with POROS™ 20 µm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

CaptureSelect™ KappaXL Affinity Matrix Thermo Scientific™

The CaptureSelect™ KappaXL Affinity Matrix has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments.

Features of this affinity matrix include:

• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies, for example
• Excellent scalability
• Non-animal-derived

CaptureSelect™ KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ KappaXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 29–38 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric acid, pH 3.5
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

POROS™ CaptureSelect™ FSH Affinity Column, 4.6 x 50 mm Thermo Scientific™

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ FSH HPLC columns incorporate the advantages of high-speed POROS™ chromatography resins with the unique selectivity of CaptureSelect™ ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

• Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ FSH affinity ligand with POROS™ 20 µm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

POROS™ Heparin 50 µm Column, 4.6 x 100 mm, 1.7 mL Thermo Scientific™

Applied Biosystems™ POROS™ 50 micron Heparin, Affinity media. POROS™ Heparin is based on an immobilized Heparin functional group designed for high throughput purification of proteins or viruses with specific Heparin affinity.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

CaptureSelect™ IgG-Fc (ms) Affinity Matrix Thermo Scientific™

CaptureSelect™ IgG-Fc (ms) Affinity Matrix has been specifically designed to purify IgG from multiple species by high affinity binding to the Fc region of IgGs . Unlike protein G, gentle elution conditions can be used to recover the product. This affinity matrix can be used for purification of IgG from a broad range of species, such as human, mouse, rat, rabbit, cow, horse, and sheep. A complete list of species can be found in the product information sheet.

POROS™ 50 OH Hydroxyl Activated Resin Thermo Scientific™

Applied Biosystems™ POROS™ Immobilized Hydroxyl Affinity Bulk Media. POROS™ OH is based on an hydroxylated functional group designed for the immobilization of proteins through activation with tresyl-chloride, divinyl-sulphone or cyanogen bromide. 50 micron bulk resins is available for low pressure applications, including large scale downstream bioprocessing.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ CaptureSelect™ FSH Affinity Column, 4.6 x 100 mm Thermo Scientific™

Analytical teams that support biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS™ CaptureSelect™ FSH HPLC columns incorporate the advantages of high-speed POROS™ chromatography resins with the unique selectivity of CaptureSelect™ ligands, enabling 3-minute quantitation of human follicle stimulating hormone (FSH).

Features of this class of chromotography columns:

• Broad utility—POROS™ A, G, and CaptureSelect™ affinity ligands address most biological drug classes
• Rapid—POROS™ resins offer fast quantitation or purification of immunoglobulins and fusion proteins
• Hands-off operation—HPLC method can be automated for streamlined operation
• Robust—delivers high-performance purification over hundreds of chromatography cycles

High Speed with High Specificity
The combination of CaptureSelect™ FSH affinity ligand with POROS™ 20 µm resin provides a high flow-rate, high performance chromatography solution for the rapid quantitation and small-scale purification of FSH.

POROS™ high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in less than 3 minutes. Moreover, only minimal sample preparation (0.2 µm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.

Automation
POROS™ affinity chromatography columns are suitable for use with HPLC systems, allowing for the automation of chromatography, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.

Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime results in strong re-use performance. POROS™ affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.

CaptureSelect™ C-tagXL Affinity Matrix Thermo Scientific™

CaptureSelect C-tagXL Affinity Matrix combines a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine) with the benefits of a robust and high quality affinity matrix. It purifies C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmatic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high activity recoveries of pH-sensitive target proteins. C-tagXL Affinity Matrix recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

Features of C-tagXL Affinity Matrix include:
• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect C-tagXL Affinity Matrix purifies C-terminal tagged E-P-E-A proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling of the ligand
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, and contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4, or 0.1 M glycine pH 3.0; Neutral: 20 mM Tris, 2.0 M MgCl2 pH 7; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris 2 mM S-E-P-E-A.
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol

CaptureSelect™ KappaXP RoboColumn Thermo Scientific™

CaptureSelect RoboColumns™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect KappaXP matrix recognizes the kappa light-chain of human antibodies, enabling purification of all Kappa containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include
:• Purification of Kappa monoclonal antibodies, Fab, and Fab2 fragments in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human-specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH (limited exposure)
• Non-animal derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created using a proprietary technology based on camelid-derived single-domain antibody fragments. The KappaXP ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics

Column size: 200 µL, 1 strip, 8 columns (8-row)
Column dimension: 5-mm diameter, 10-mm bed height
Matrix: agarose based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide coupling
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH6
Formulation buffer: 20% (v/v) ethanol
Max operating pressure: 2 bar
Flow rate: 16–150 cm/hr

RoboColumn is a trademark of Repligen.

CaptureSelect™ KappaXP Affinity Matrix Thermo Scientific™

CaptureSelect KappaXP Affinity Matrix is the improved version of CaptureSelect KappaXL Affinity Matrix, with a significant increase in binding capacity for both whole kappa monoclonal antibodies and kappa Fab's. It enables efficient elution, even at pH 6.0, and demonstrates an improved stability for caustic cleaning agents. It is specific for human antibodies or antibody fragments, without cross-specificity to bovine antibody.

CaptureSelect KappaXP matrix recognizes the kappa light chain of human antibodies, enabling purification of all kappa-containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.

CaptureSelect KappaXP Affinity Matrix features include:
• Purification of kappa monoclonal antibodies, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Non–animal-derived

The high selectivity and high yields obtained using CaptureSelect KappaXP Affinity Matrix enable a robust and efficient purification process with excellent purity in one step.

View other antibody therapeutic affinity matrix formats ›

Free of animal components
CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single domain antibody fragments. The KappaXP ligand is a 13-kDa single-domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: epoxide immobilization of the ligand
Fab binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and residence time
IgG binding capacity: 35–45 g IgG/liter resin depending on flow rate, column height, and residence time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2, pH 6
Flow characteristics: 150–300 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

HisPur™ Cobalt Superflow Agarose Thermo Scientific™

Thermo Scientific HisPur Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

Features of HisPur Cobalt Superflow Agarose:

Robust—highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200 cm/hour with minimal leaching of cobalt due to a tetradentate chelator
High yields—binds 20 mg of 6xHis-GFP per mL of resin at a linear flow rate of 150 cm/hour, can bind greater than 30 mg per mL of resin depending on flow rate
High purity—specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
Compatible—maintains function after exposure to a wide variety of chemicals and pH values
Cost effective—resin is stable through multiple cycles of cleaning and reuse

HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps.

Applications:
• Large scale FPLC purification of polyhistidine tagged proteins

Affinity chromatography is often used as a quick and easy approach for the purification of recombinant proteins. One such method of purification involves the binding of a polyhistidine tag to a divalent metal cation that has been coordinated by a chelator immobilized on a beaded support. For immobilized metal affinity chromatography (IMAC) purification of His-tagged proteins, the type of bead, chelator, and metal immobilized influences purity, yield, and flow rate performance of these purifications.

The highly crosslinked 6% agarose Superflow beaded support tolerates larger linear flow rates than agarose resins that have lower amounts of crosslinking. The HisPur Cobalt Superflow Agarose protocol utilizes gentle wash and elution conditions and typically produces greater than 90% pure target protein. Protein purities achieved with HisPur Cobalt Superflow Agarose are generally higher than those achieved with nickel IMAC resins making HisPur Cobalt Superflow Agarose a valuable tool for users interested in one-step purifications. Additionally, HisPur Cobalt Superflow Agarose is perfect for larger scale purifications that require the resin to withstand medium pressures without compressing.

More Product Data
Performance characterization of HisPur Cobalt Superflow Agarose

Related Products
HisPur™ Cobalt Resin

CaptureSelect™ IgA Affinity Matrix Thermo Scientific™

The CaptureSelect™ IgA Affinity Matrix has specifically been designed for the purification of human IgA from recombinant and plasma sources.

Features of this affinity matrix include:

• Platform for all human IgA, binding to IgA1, IgA2 , dimeric IgA, and S-IgA (secretory IgA)
• Excellent scalability
• Non-animal-derived

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ IgA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~8 g/L resin
Elution conditions: 0.1 M glycine, pH 3.0
Flow characteristics: 200–400 cm/h
Formulation buffer: 20%(v/v) ethanol

CaptureSelect™ Human Growth Hormone Affinity Matrix Thermo Scientific™

CaptureSelect Human Growth Hormone (hGH) Affinity Matrix has been specifically designed for the purification of human growth hormone from recombinant sources.

Features of this affinity matrix include:
• Highly selective for recombinant hGH
• Efficient purification of recombinant hGH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Recombinant hGH, also known as somatotropin or somatropin, is a peptide hormone that stimulates growth, cell reproduction, and regeneration in humans and other animals. The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

View other biosimilar and recombinant protein affinity matrix formats ›

Free of animal components
Our CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect hGH affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~3 mg/mL resin
Elution conditions: 20 mM citric acid, pH 3.0
Flow characteristics: 50–150 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol

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