Shop All LC Reversed-Phase Columns, Media, & Standards

Syncronis™ C18 Columns Thermo Scientific™

Achieve repeatable and consistent liquid chromatography (LC) separations using Thermo Scientific™ Syncronis™ C18 columns. Highly pure, high surface area silica and dense stationary phase coverage minimize secondary interactions and ensure robust, high-resolution separations. Double end-capping provides extra surface coverage and inertness toward basic compounds. Manufactured and rigorously tested in ISO 9000 facilities, these columns are designed to meet a range of analytical needs including fast method development, quantitative analysis, and quality control.

Acclaim™ Phenyl-1 HPLC Columns Thermo Scientific™

For unique selectivity of aromatic compounds and superior chromatographic performance, choose Thermo Scientific™ Acclaim™ Phenyl-1 reversed-phase LC columns. The specially designed bonded phase displays greater π-π interaction than other phenyl phases and provides enhanced aromatic selectivity and higher hydrophobic retention. These columns are ideally suited for the analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

EASY-Spray™ HPLC Columns Thermo Scientific™

Ensure robust nano and capillary flow LC-MS with Thermo Scientific™ EASY-Spray™ LC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigourously tested to ensure maximum quality, these columns are designed for maximum simplicity and ease-of-use.

Acclaim™ Polar Advantage II HPLC Columns Thermo Scientific™

Resolve polar and nonpolar compounds in a single run with Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) reversed-phase columns. These high-efficiency, silica-based columns have a polar-embedded stationary phase that operates over a wider range of chromatographic conditions than possible with conventional reversed-phase stationary phases. Its unique chemistry provides enhanced hydrolytic stability from pH 1.5 to 10 with 100% aqueous mobile phases. The column exhibits selectivity that is complementary to conventional C18 columns and excellent peak shapes for both basic and acidic compounds.

BETASIL™ C18 Preparative HPLC Columns Thermo Scientific™

Deliver the high surface area with high bonded phase coverage for your scale up purification needs with Thermo Scientific™ BETASIL™ C18 preparative HPLC columns. Our columns come in a wide range of dimensions to suit your application needs.

Hypersil™ BDS C18 Columns Thermo Scientific™

For a robust, general-purpose column to use in a wide range of applications, choose Thermo Scientific™ Hypersil™ BDS C18 HPLC Columns. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ columns as an alternative.

Hypersil™ BDS C8 HPLC Columns Thermo Scientific™

For a high-quality, base-deactivated, fully endcapped phase with similar selectivity to C18 but slightly less retention, use Thermo Scientific™ Hypersil™ BDS C8 HPLC Columns. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ C8 columns as an alternative.

Hypersil GOLD™ aQ C18 Polar Endcapped HPLC Columns Thermo Scientific™

Retain and resolve polar analytes using Thermo Scientific™ Hypersil GOLD™ aQ Polar Endcapped C18 columns. These columns provide excellent peak shape, even with challenging reversed phase separations using highly aqueous mobile phases.

Hypersil GOLD™ C4 HPLC Columns Thermo Scientific™

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.

Hypersil GOLD™ VANQUISH™ PFP UHPLC Columns Thermo Scientific™

Achieve even greater power of separation, speed, and throughput for analyzing difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds by using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ PFP (pentafluorophenyl) UHPLC columns. These columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges of analyzing complex matrices using liquid chromatography (LC) and LC-MS.

Hypersil GOLD™ C18 Selectivity HPLC Columns Thermo Scientific™

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.

Hypersil GOLD™ Phenyl HPLC Columns Thermo Scientific™

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns. A butyl (C4) linker in the phenyl bonded phase allows for superior alignment of the phenyl ring with aromatic molecules, thus enhancing pi-pi interactions and improving the retention of the molecules. The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. The outstanding peak shape results in greater sensitivity.

Accucore™ XL C18 HPLC Columns Thermo Scientific™

Optimize conventional HPLC methods with Thermo Scientific™ Accucore™ XL C18 LC Columns. Rugged 4µm solid-core particles ensure very high efficiencies within standard HPLC pressure limits. The high bonded phase coverage provides optimal retention of a broad range of nonpolar analytes across multiple applications. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.

POROS™ R2 10 µm Column, Stainless Steel, 4.6 x 100 mm, 1.7 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Accucore™ PFP HPLC Columns Thermo Scientific™

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. Rugged 2.6µm solid-core particles enable fast, high-resolution separations at low backpressures. Fluorine groups in the PFP stationary phase provide enhanced retention and selectivity for positional isomers of halogenated compounds and for compounds with polar functional groups. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.
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