Shop All Other Reversed Phase LC Columns, Media, & Standards

Acclaim™ Polar Advantage II LC Columns (Thermo Scientific™)

Resolve polar and nonpolar compounds in a single run with these enhanced hydrolytic stabile (pH 1.5–10) Thermo Scientific™ Acclaim™ PolarAdvantage II reversed-phase columns.

OLIGO™ R3 Column;, 4.6 mm x 100mm, 1.7 ml (Applied Biosystems™)

Thermo Scientific™ Oligo™ R3 bulk resin is a rigid polymeric bead designed for reversed-phase chromatography of polynucleotides and other biomolecules. The combination of 30-μm particle size, resistance to high pH, and high-phase ratio, makes Oligo R3 resin particularly suited to preparative purification of synthetic oligonucleotides and peptides. The packing consists of cross-linked poly-divinylbenzene particles. Oligo R3 bulk resin differs somewhat in selectivity from conventional silica-based, reversed-phase resins and is similar to high-carbon-loading through C18 supports. In addition, the complete lack of residual silanol activity may modify the selectivity of ionic species.

Features of Oligo R3 resin and pre-packed columns include:
• High performance chromatography resin for analytical to process scale separations
• High resolution preparative purification at high flow rates
• Chemical stability—allows aggressive cleaning and sanitization
• Poly-divinylbenzene particles—yield a robust, easily packable matrix
• Combines low back pressure, high pH stability, and high capacity

ProSwift™ C4 RP-5H Capillary LC Columns (Thermo Scientific™)

Improve speed and resolution of LC-MS protein identification and biomarker discovery using these C4 monolithic HPLC columns.

Hypersil GOLD™ Phenyl LC Columns (Thermo Scientific™)

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns.

POROS™ R2 10 µm Column, Stainless Steel, 2.1 x 100 mm, 0.3 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

POROS™ R1 20 µm Column, Stainless Steel, 4.6 x 100 mm, 1.7 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R1 is a 4000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Hypercarb™ Porous Graphitic Carbon LC Columns (Thermo Scientific™)

Offer 100% porous graphitic carbon for extended separation capabilities with Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon LC Columns.

Accucore™ RP-MS LC Columns (Thermo Scientific™)

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.

Hypersil GOLD™ VANQUISH™ PFP UHPLC Columns (Thermo Scientific™)

Use these columns for better, faster, easier separation of halogenated and non-halogenated polar compounds in complex mixtures.

POROS™ R2 10 µm Column, Stainless Steel, 4.6 x 50 mm, 0.8 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

OLIGO™ R3 Column, Stainless Steel, 4.6 mm x 100mm, 1.7 ml (Applied Biosystems™)

Thermo Scientific™ Oligo™ R3 bulk resin is a rigid polymeric bead designed for reversed-phase chromatography of polynucleotides and other biomolecules. The combination of 30-μm particle size, resistance to high pH, and high-phase ratio, makes Oligo R3 resin particularly suited to preparative purification of synthetic oligonucleotides and peptides. The packing consists of cross-linked poly-divinylbenzene particles. Oligo R3 bulk resin differs somewhat in selectivity from conventional silica-based, reversed-phase resins and is similar to high-carbon-loading through C18 supports. In addition, the complete lack of residual silanol activity may modify the selectivity of ionic species.

Features of Oligo R3 resin and pre-packed columns include:
• High performance chromatography resin for analytical to process scale separations
• High resolution preparative purification at high flow rates
• Chemical stability—allows aggressive cleaning and sanitization
• Poly-divinylbenzene particles—yield a robust, easily packable matrix
• Combines low back pressure, high pH stability, and high capacity

Syncronis™ Phenyl LC Columns (Thermo Scientific™)

Maximize liquid chromatography reproducibility and laboratory productivity using Thermo Scientific™ Syncronis™ Phenyl LC Columns.

Hypersil GOLD™ PFP LC Columns (Thermo Scientific™)

Analyze difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds with Thermo Scientific™ Hypersil GOLD™ PFP (pentafluorophenyl) HPLC columns.

Hypersil GOLD™ C4 LC Columns (Thermo Scientific™)

Featuring a lower hydrophobicity than C18 or C8 columns, Thermo Scientific™ Hypersil GOLD™ C4 columns provide outstanding sensitivity and high efficiency.

POROS™ R2 10 µm Column, Stainless Steel, 4.6 x 100 mm, 1.7 mL (Thermo Scientific™)

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.