Shop All Other Reversed Phase LC Columns, Media, & Standards

Acclaim™ Phenyl-1 HPLC Columns Thermo Scientific™

For unique selectivity of aromatic compounds and superior chromatographic performance, choose Thermo Scientific™ Acclaim™ Phenyl-1 reversed-phase LC columns. The specially designed bonded phase displays greater π-π interaction than other phenyl phases and provides enhanced aromatic selectivity and higher hydrophobic retention. These columns are ideally suited for the analysis of aromatic analytes, including glucocorticosteroids, estrogens, fat-soluble vitamins, and phospholipids.

Acclaim™ Polar Advantage II HPLC Columns Thermo Scientific™

Resolve polar and nonpolar compounds in a single run with Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) reversed-phase columns. These high-efficiency, silica-based columns have a polar-embedded stationary phase that operates over a wider range of chromatographic conditions than possible with conventional reversed-phase stationary phases. Its unique chemistry provides enhanced hydrolytic stability from pH 1.5 to 10 with 100% aqueous mobile phases. The column exhibits selectivity that is complementary to conventional C18 columns and excellent peak shapes for both basic and acidic compounds.

Hypersil GOLD™ C4 HPLC Columns Thermo Scientific™

For very hydrophobic analytes, achieve faster separations with excellent peak shapes using Thermo Scientific™ Hypersil GOLD™ C4 HPLC columns. Featuring a lower hydrophobicity than C18 or C8 columns, Hypersil GOLD C4 columns provide outstanding sensitivity and high efficiency.

Hypersil GOLD™ VANQUISH™ PFP UHPLC Columns Thermo Scientific™

Achieve even greater power of separation, speed, and throughput for analyzing difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds by using Thermo Scientific™ Hypersil GOLD™ VANQUISH™ PFP (pentafluorophenyl) UHPLC columns. These columns were developed in conjunction with the Vanquish UHPLC system to take advantage of the system's extended pressure capabilities and robustness. The result is a new level of separation, speed and throughput that solve analytical challenges of analyzing complex matrices using liquid chromatography (LC) and LC-MS.

Hypersil GOLD™ Phenyl HPLC Columns Thermo Scientific™

Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns. A butyl (C4) linker in the phenyl bonded phase allows for superior alignment of the phenyl ring with aromatic molecules, thus enhancing pi-pi interactions and improving the retention of the molecules. The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity. The outstanding peak shape results in greater sensitivity.

POROS™ R2 10 µm Column, Stainless Steel, 4.6 x 100 mm, 1.7 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.

Accucore™ PFP HPLC Columns Thermo Scientific™

Improve LC separations of halogenated compounds and nonhalogenated polar analytes using Thermo Scientific™ Accucore™ PFP LC columns. Rugged 2.6µm solid-core particles enable fast, high-resolution separations at low backpressures. Fluorine groups in the PFP stationary phase provide enhanced retention and selectivity for positional isomers of halogenated compounds and for compounds with polar functional groups. Robust bonding technology and automated packing procedures ensure excellent reproducibility and long column lifetimes.

Hypersil GOLD™ PFP HPLC Columns Thermo Scientific™

Analyze difficult-to-resolve mixtures of halogenated compounds and non-halogenated polar compounds with Thermo Scientific™ Hypersil GOLD™ PFP (pentafluorophenyl) HPLC columns. These columns provide an alternative selectivity to C18 columns in reversed phase applications with excellent peak shape and sensitivity.

Hypercarb™ Porous Graphitic Carbon HPLC Columns Thermo Scientific™

Offer 100% porous graphitic carbon for extended separation capabilities with Thermo Scientific™ Hypercarb™ Porous Graphitic Carbon LC Columns. Enhanced retention of polar compounds and separation of structurally related analytes with stability at pH extremes and high temperature.

Acclaim™ Polar Advantage HPLC Columns Thermo Scientific™

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes. In addition, the mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negates the problems of dewetting common with conventional C18 reversed-phase columns. Use Acclaim PA columns for efficient separations of nucleic acid and bases or pharmaceutical compounds using highly aqueous mobile phases.

Hypersil™ Phenyl-BDS HPLC Columns Thermo Scientific™

As a base-deactivated column with unique selectivity for aromatic and slightly polar compounds, you can use Thermo Scientific™ Hypersil™ Phenyl-BDS LC Columns as an alternative selectivity to C18 or C8 phases. These economical columns feature reduced silanol activity and provide exceptional reliability for QA/QC labs. Built on the renowned Hypersil silica backbone, the base-deactivated silica is one of the most popular packing materials available.

 

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ columns as an alternative.

Hypersil™ Phenyl HPLC Columns Thermo Scientific™

Effectively separate aromatic compounds and moderately polar compounds with Thermo Scientific™ Hypersil™ Phenyl LC Columns.

Classical Hypersil stationary phases are recognized as an industry standard in HPLC. Proven as an effective analytical tool, classical Hypersil phases are well established and referenced in HPLC methods. Quality is assured by manufacture under ISO 9001-2000 quality guidelines.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ Phenyl columns as an alternative.

Hypersil™ Phenyl-2 HPLC Columns Thermo Scientific™

Separate aromatic compounds and moderately polar compounds with Thermo Scientific™ Hypersil™ Phenyl-2 LC Columns.

Classical Hypersil stationary phases are recognized as an industry standard in HPLC. Proven as an effective analytical tool, classical Hypersil phases are well established and referenced in HPLC methods. Quality is assured by manufacture under ISO 9001-2000 quality guidelines.

For more challenging separations that require improved chromatographic performance, use Thermo Scientific™ Hypersil GOLD™ Phenyl columns as an alternative.

Accucore™ RP-MS HPLC Columns Thermo Scientific™

Optimized for MS detection, Thermo Scientific™ Accucore™ RP-MS LC Columns are an excellent combination of speed and quality of separation.

Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations—without the elevated backpressures required by sub-2μm particles.

POROS™ R2 10 µm Column, 4.6 x 100 mm, 1.7 mL Thermo Scientific™

Applied Biosystems™ POROS™ Prepacked Reversed-Phase Column. POROS™ R2 is a 2000 angstrom pore size Poly[styrenevinylbenzene] particle, suitable for the reversed-phase separation of biomolecules.
10 micron particle size is used for the highest resolution chromatography, and is suitable for analytical to small scale preparative separation of biomolecules.

POROS™ High Performance Chromatography™ Media and Pre-Packed Columns

A high performance chromatography resins for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High capacity, high resolution, high speed
In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
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